Sensitivity comparison of Beckman ASQ HIV Recombinant ELISA and Organon Teknika Western Blot.

Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

Sensitivity comparison of Beckman ASQ HIV Recombinant ELISA and Organon Teknika Western Blot.

Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:343 (abstract no. T-41). Unique Identifier : AIDSLINE ASM90/905038
Condie T; Mottice S; Bureau of Microbiology, Utah State Health Laboratory, 44 Medical; Drive S.L.C., UT, 84113

Abstract: A comparison of sensitivity was made between the Beckman ASQ HIV Recombinant ELISA assay, which tests for a panel of the six viral peptides, p24, kp55, kp41, kp66/31, kp120N and kp 120CC, and the Organon Teknika HIV Western Blot. Serum specimens showing strong antibody reaction to viral proteins p17, p24, gp41, p51, p55, p66, gp120, and gp 160 on Organon Teknika Western Blot strips were diluted and tested in a DuPont EIA. Thirty-eight serum dilutions having an O.D. reading +/- 20% of DuPonts' EIA reactive threshold value were assayed using the Beckman ASQ Recombinant ELISA and the Organon Teknika Western Blot. Results show equal sensitivity between the recombinant ELISA and the Western Blot for antibodies to p24 and p66. The recombinant ELISA has greater sensitivity over the Western Blot for p31, gp41, and p55 with 92%, 82%, and 40% detection rate of the total number of positive samples, respectively, compared to 58%, 76%, and 30% for the Western Blot. The recombinant ELISA has very low sensitivity for the 120 envelope structural proteins with 8% detection rate of the total number of positives for 120N and 5% for 120CC compared to 100% detection rate with the Western Blot. The current criteria for interpretation of HIV positivity from the Centers for Disease Control is evidence of antibodies to any two of: p24, gp41, and gp120/gp160 antigens. The low sensitivity of the ASQ Recombinant ELISA for antibodies to the 120 class of viral proteins limits a positive interpretation to the presence of p24 and gp41. We believe the quantity and quality of information provided by the Organon Teknika Western Blot is superior to that of the Beckman ASQ Recombinant ELISA.

Keywords: *Blotting, Western Centers for Disease Control and Prevention (U.S.) Comparative Study *Enzyme-Linked Immunosorbent Assay Human *HIV/IMMUNOLOGY HIV Antibodies/IMMUNOLOGY HIV Infections/*DIAGNOSIS Recombinant Proteins/IMMUNOLOGY Reproducibility of Results *Sensitivity and Specificity Spectrophotometry United States Viral Envelope Proteins/IMMUNOLOGY ABSTRACT
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