Extrachromosomal HIV-1 DNA in persistently infected U937 cells. NLM AIDSLINE Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

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Extrachromosomal HIV-1 DNA in persistently infected U937 cells.

AIDS Res Hum Retroviruses. 1990 Aug;6(8):1027-30. Unique Identifier : AIDSLINE MED/91026267
Pauza CD; Singh MK; Developmental Biology Laboratory, Salk Institute for Biological; Studies, San Diego, CA 92138-9216.

Abstract: Persistent human immunodeficiency virus type 1 (HIV-1) infection of U937 monocytic cells resulted in the accumulation of novel forms of extrachromosomal viral DNA. These DNA species are larger than the genome size of HIV-1 and persist indefinitely. The extrachromosomal viral DNA species (E-DNA) were shown to be structurally stable by subcloning of infected cell lines and restriction fragment analysis. Similar E-DNA structures were observed in independent infections. Persistently infected monocytic cells had low levels of viral antigens, reflecting the low levels of viral RNA that were detected. These results support a role for E-DNA in persistent HIV-1 infection of monocytic cells.
Keywords: Cell Line DNA, Viral/*METABOLISM *Extrachromosomal Inheritance Human HIV-1/*GENETICS Monocytes/*MICROBIOLOGY RNA, Messenger/METABOLISM RNA, Viral/*BIOSYNTHESIS Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. JOURNAL ARTICLEKWDcelllinedna,viral/KWDmetabolismKWDextrachromosomalinheritancehumanhiv-1/KWDgeneticsmonocytes/KWDmicrobiologyrna,messenger/metabolismrna,viral/KWDbiosynthesissupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDjournalarticle
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Copyright © 1991 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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