ORGAN DISTRIBUTION OF FOLATES IN TRANSGENIC MICE WITH TUMORS AND IN NONTRANSGENIC LITTERMATES (MEETING ABSTRACT)

Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

ORGAN DISTRIBUTION OF FOLATES IN TRANSGENIC MICE WITH TUMORS AND IN NONTRANSGENIC LITTERMATES (MEETING ABSTRACT)

FASEB J; 5(6):A1639 1991. Unique Identifier : AIDSLINE ICDB/91676495
Bills ND; Hinrichs SH; Clifford AJ; Dept. of Nutrition, Univ. of California, Davis, CA 95616

Abstract: Transgenic mice (TM) with known predisposition to develop neoplasia (Science 237:1340-3, 1987) were fed a cereal-based diet since weaning and used to evaluate the effects of cancer on distribution of total body folate among major host organs and tumors. TM have germ line transmission of a transgene incorporating the long terminal repeats from human T-cell leukemia virus that control expression of the tax gene whose expression leads to characteristic mesenchymal tumors arising from the peripheral nerve sheath. A key morphologic feature is early infiltration of tumors by neutrophils and spleen enlargement. Mice were genotyped at 10 days of age; all TM have tumors at 90-130 days of age, and nontransgenic littermates are controls. When tumor burden of TM was approx 5%, mice were bled for hematology, whole blood and serum folate measurements. Liver, spleen, kidneys, brain and tumors were excised and analyzed for folate. Liver and spleen folate levels were similar between TM and controls. TM liver weights were approx 80% those of controls; TM spleen weights were approx twice those of controls. Total liver folate in TM was approx 80%, while total spleen folate was 240% that of controls. Data show organ redistribution of folate in TM with tumors. TM offer valuable opportunities to study nutrition and cancer.

Keywords: Animal Cell Transformation, Neoplastic/*GENETICS Folic Acid/*METABOLISM Gene Expression Regulation, Neoplastic/*PHYSIOLOGY Genes, pX/*GENETICS *Genotype Mice Mice, Transgenic Neoplasms, Experimental/*GENETICS/METABOLISM Tissue Distribution ABSTRACT
912130
M91C4077

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