1991

AIDS. [videorecording]: Houston, Tex. : Gulf Pub. Co. Video, c1988 1 videocassette : sd., col. ; 1/2 in (Clinical issues in medicine) Unique Identifier : AIDSLINE AV/9101381A; Gotto AM; Greenberg SB
AIDS, hepatitis B : what's your risk. [videorecording]: Houston, Tex. : The College, c1988 1 videocassette (22 min.) : sd., col. ; 1/2 in Unique Identifier : AIDSLINE AV/9101382A
Teen AIDS in focus. [videorecording]: [San Francisco, Calif.] : City and County of San Francisco, San Francisco Dept. of Public Health, San Francisco Unified School District, c1989 1 videocassette (16 min., 30 sec.) : sd., col. with b&w ; 1/2 in Unique Identifier : AIDSLINE AV/9101321A; (Producer) This video is an approach to personalizing acquired immunodeficiency syndrome (AIDS) for teens, young adults, their educators, parents, and mentors. The video introduces viewers to three young people with human immunodeficiency virus (HIV) infection who talk openly about their lives, relationships, and pe
AIDS and the second sex ; What is a family ; A Question of civil rights. [videorecording]: [Boston, Mass.] : WGBH Educational Foundation, c1989 1 videocassette (58 min.) : sd., col. with b&w ; 1/2 in Unique Identifier : AIDSLINE AV/9101325A; Jennings P; (Producer) Among minorities, new cases of AIDS are reported at a rate six times greater than among whites. At the same time, minority access to health care is limited, at best, and federal money earmarked for the AIDS programs is not reaching minority communities devastated by the disease.
The:Education of Admiral Watkins ; AIDS update ; A Death in the family. [videorecording]: [Boston, Mass.] : WGBH Educational Foundation, c1989 1 videocassette (59 min.) : sd., col. with b&w ; 1/2 in Unique Identifier : AIDSLINE AV/9101339A; Jennings P; (Producer) Retired Admiral James Watkins, a former Chief of United States Naval Operations with no health policy experience, was appointed chairman of the Presidential Commission on the Human Immunodeficiency Virus (HIV) Epidemic by President Ronald Reagan in late 1987. Within eight
Techniques for the prevention of HIV and hepatitis infection in health care workers. Rev. [videorecording]: Research Triangle Park, NC : Glaxo, 1989 1 videocassette (11 min., 45 sec.) : sd., col. ; 1/2 in (Masters of medicine) Unique Identifier : AIDSLINE AV/9101416A; Mills J; Luce JM; Gerberding J
The:Psychology of treating patients with HIV disease. [videorecording]: [Research Triangle Park, N.C.] : Burroughs Wellcome Co., c1989 1 videocassette : sd., col. ; 1/2 in. + 1 guide Unique Identifier : AIDSLINE AV/9101455A; McKusick L
2nd Pan American Teleconference on AIDS. [videorecording]: Washington, D.C. : PAHO, c1989 5 videocassettes (6 hr., 6 min., 25 sec.) : sd., col. ; 1/2 in Unique Identifier : AIDSLINE AV/9101465A; St. John RK
Stopping AIDS, stopping drugs ; The Art of medicine. [videorecording]: [Boston, Mass.] : WGBH Educational Foundation, [1989] 1 videocassette (58 min.) : sd., col. ; 1/2 in Unique Identifier : AIDSLINE AV/9101367A; Jennings P; (Producer) In this episode, the news about AIDS began to contain a clear reason for hope. Research scientists predicted that within several years, AIDS would become a chronic and serious but manageable illness. While scientists searched for an effective treatment, AIDS spread to new populations. In one segment, th
The:Crisis in Poland ; To live with AIDS. [videorecording]: [Boston, Mass.] : WGBH Educational Foundation, c1990 1 videocassette (58 min.) : sd., col. with b&w ; 1/2 in Unique Identifier : AIDSLINE AV/9101331A; Jennings P; (Producer) ABC news anchor Peter Jennings takes a journey across Poland to probe some of the reasons behind this country s vulnerability to AIDS including the fact that in this predominantly Roman Catholic country, condoms are not
The:Crisis in Texas ; Prisoners of AIDS. [videorecording]: [Boston, Mass.] : WGBH Educational Foundation, c1990 1 videocassette (57 min.) : sd., col. ; 1/2 in Unique Identifier : AIDSLINE AV/9101332A; Jennings P; (Producer) Provincial morals and a scarcity of health care professionals, medicines and support groups make living with AIDS in a small community particularly difficult. The program also delves into the impact of AIDS on prison populations.
The:Trial of compound Q ; Money and morals. [videorecording]: [Boston, Mass.] : WGBH Educational Foundation, c1990 1 videocassette (59 min.) : sd., col. with b&w ; 1/2 in Unique Identifier : AIDSLINE AV/9101372A; Jennings P; (Producer) After years of criticizing the Food and Drug Administration for its lengthy testing policies, AIDS activists decided to test the experimental Compound Q on themselves. Without FDA sanction or oversight, the activists set out to prove that drug trials could be conducted mor
AIDS in otolaryngology : head and neck surgery. [videorecording]: Washington, D.C. : Health Video Dynamics, c1990 1 videocassette : sd., col. with b&w ; 1/2 in (New perspectives in otolaryngology--head and neck surgery) Unique Identifier : AIDSLINE AV/9101421A; Lucente FE
Changing focus : women, children, and AIDS in the 90's. [videorecording]: Green Bay, WI : NEWIST/CESA no. 7, c1990 1 videocassette (20 min.) : sd., col. ; 1/2 in. + 1 guide Unique Identifier : AIDSLINE AV/9101425A; Littig D; of Wisconsin--Green Bay)
The:Science of hope with Jonas Salk. [videorecording]: [New York, N.Y.] : Public Affairs Television, c1990 1 videocassette (28 min.) : sd., col. ; 1/2 in Unique Identifier : AIDSLINE AV/9101460A; Salk J; Moyers BD; (Producer) Jonas Salk is the developer of the polio vaccine and founder of the Salk Institute in La Jolla, California. He talks about AIDS research and how the technique of negotiating with the AIDS virus offers a way of thinking about human beings as part of, not apart from nature. Salk feels the development of an
Protecting yourself from AIDS for health care workers. [videorecording]: Fullerton, CA : SAVANT ; [United States] : Prudential Insurance Company of America : Midnight Media Group, c1991 2 videocassettes (36 min.) : sd., col. ; 1/2 in. + 1 guide Unique Identifier : AIDSLINE AV/9101360A
Protecting yourself from AIDS for laboratory workers. [videorecording]: Fullerton, CA : SAVANT ; [United States] : Prudential Insurance Company of America : Midnight Media Group, c1991 2 videocassettes (39 min.) : sd., col. ; 1/2 in. + 1 guide Unique Identifier : AIDSLINE AV/9101361A
The:Gospel alive : caring for persons with AIDS and related illnesses : a pastoral document.: the United States St. Louis, MO : Catholic Health Association of the United States ; Silver Spring, MD : Conference of Major Religious Superiors of Men's Institutes of the United States, 1988 xii, 128 p Unique Identifier : AIDSLINE CAT/9113738
[L'ARCAT-SIDA : recherche, information, formation, soutien aux malades.]: sa therapeutique Paris : Association pour la recherche clinique contre l'AIDS-SIDA et pour sa therapeutique, [1988] [16] leaves : ill Unique Identifier : AIDSLINE CAT/9113350
[SPID : sindrom priobretennogo immunnogo defitsita.]: Minsk : Belarus', 1988 125 p., [4] p. of plates : ill., ports Unique Identifier : AIDSLINE CAT/9113115; Rytik PG
AIDS update.: Reston, Va. : Cancer Letter, Inc., v Unique Identifier : AIDSLINE CAT/9113945
1989 Bragg mortality/AIDS report.: Atlanta, Ga. (28 Lenox Pointe, N.E., Atlanta 30324) : Bragg Associates, c1989 61 leaves Unique Identifier : AIDSLINE CAT/9113872
[Oeil et SIDA.]: Paris : Doin, c1989 vii, 92 p., 16 p. of plates : ill (Monographie de la Societe francaise d'ophtalmologie) Unique Identifier : AIDSLINE CAT/9113409; Le Hoang P
[Le:SIDA : un nouveau defi medical.]: [Montreal] : Association des medecins de langue francaise du Canada, 1989 printing 99 p. : ill Unique Identifier : AIDSLINE CAT/9113470; Olivier C
Sex education and AIDS education in the schools : a survey of state policies, curricula, and program activities.: New York, N.Y. (111 Fifth Ave., New York 10003) : Alan Guttmacher Institute, [1989?] 1 v. (various pagings) Unique Identifier : AIDSLINE CAT/9114362; Kenney A
Epidemiologists, social scientists, and the structure of medical research on AIDS in Africa.: Boston, MA (270 Bay State Rd., Boston 02215) : African Studies Center, Boston University, 1989 19 leaves : ill (Working papers in African studies ; no. 137) Unique Identifier : AIDSLINE CAT/9112867; Packard RM
AIDS : the African perspective of the killer disease.: Accra : Ghana Universities Press, 1989 xiii, 206 p. : ill Unique Identifier : AIDSLINE CAT/9113201; Agadzi VK
[SIDA : mitos y realidades.]: San Jose, Costa Rica : Euroamericana de Ediciones, C.R., 1990 printing xi, 117 p Unique Identifier : AIDSLINE CAT/9113469; Frajman M
[Un:defi mondial : le SIDA.]: Aubervilliers : Documentation francaise, [1990] 64 p (Problemes politiques et sociaux, ISSN 0015-9743 ; no 625) Unique Identifier : AIDSLINE CAT/9113156; Pierret J
Community-based care of persons with AIDS : developing a research agenda : AHCPR conference proceedings, Minneapolis, Minnesota, August 15-16, 1989.: Rockville, MD : U.S. Dept. of Health and Human Services, Public Health Service, Agency for Health Care Policy and Research, [1990] viii, 181 p. : ill (DHHS publication ; no. (PHS) 90-3456) Unique Identifier : AIDSLINE CAT/9113699
Abstracts of papers presented at the 1990 meeting on modern approaches to new vaccines, including prevention of AIDS : September 12-September 16, 1990.: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory, [1990] xxv, 106 p Unique Identifier : AIDSLINE CAT/9113910; Brown F
AIDS and intravenous drug use : community intervention and prevention.: New York : Hemisphere Pub. Corp., c1990 xiv, 299 p. : ill Unique Identifier : AIDSLINE CAT/9113944; Leukefeld CG; Battjes R; Amsel Z
Identification and management of asymptomatic HIV-infected persons in New Jersey : a practical protocol for New Jersey clinicians.: Lawrenceville, NJ, USA : Academy of Medicine of New Jersey ; [Trenton, NJ] : New Jersey State Dept. of Health, c1990 1 v. (loose-leaf) Unique Identifier : AIDSLINE CAT/9114139
Inventing AIDS.: New York : Routledge, 1990 ix, 176 p Unique Identifier : AIDSLINE CAT/9114150; Patton C
Pediatric HIV infection : selected research issues.: [Washington, D.C.] : Congressional Research Service, the Library of Congress, [1990] 14 p (CRS issue brief) Unique Identifier : AIDSLINE CAT/9113803; Johnson JA
Clinical manual for care of the adult patient with HIV infection.: [Boston] : The Hospital, 1990 274, 34 p. : ill Unique Identifier : AIDSLINE CAT/9114001; Libman H; Witzburg RA
Proceedings : AIDS Prevention and Services Workshop, February 15-16, 1990, Washington D.C.: Princeton, N.J. : Robert Wood Johnson Foundation Communications Office, [1990] 192 p. : ill Unique Identifier : AIDSLINE CAT/9114321; Fransen VE; Van Wert J; Weisfeld VD
BETAbulletin of experimental treatments for AIDS : a publication of the San Francisco AIDS foundation.: [San Francisco, Calif.] : The Foundation, v. : ill., ports Unique Identifier : AIDSLINE CAT/9113964
AIDS--responses, interventions, and care.: London ; New York : Falmer Press, 1991 vii, 286 p. : ill (Social aspects of AIDS) Unique Identifier : AIDSLINE CAT/9112808; Aggleton P; Hart G; Davies P
State of the state of AIDS in New York : leadership, housing, health care, education, insurance.: New York, NY (129 W. 20th St., NY 10011) : Gay Men's Health Crisis, [1991] 12 p Unique Identifier : AIDSLINE CAT/9113838
Counseling chemically dependent people with HIV illness.: New York : Harringon Park Press, c1991 Unique Identifier : AIDSLINE CAT/9114014; Shernoff M
In the midst of winter : systemic therapy with families, couples, and individuals with AIDS infection. 1st ed.: New York : Norton, c1991 xviii, 362 p Unique Identifier : AIDSLINE CAT/9114144; Walker G
National AIDS Information Clearinghouse conference calendar. Jan. 1991-Apr. 1991.: Rockville, MD : The Clearinghouse, 1991 2 v Unique Identifier : AIDSLINE CAT/9113786
AIDS and human reproduction.: Basel ; New York : Karger, 1992 xi, 195 p. : ill Unique Identifier : AIDSLINE CAT/9113943; Melica F
The:Time of AIDS : social analysis, theory, and method.: Newbury Park : Sage Publications, c1992 ix, 341 p Unique Identifier : AIDSLINE CAT/9113850; Herdt GH; Lindenbaum S
Science challenging AIDS : proceedings based on the VIIth International Conference on AIDS, Florence, June 16-21, 1991.: Basel ; New York : Karger, 1992 xviii, 300 p. : ill Unique Identifier : AIDSLINE CAT/9113089; Giraldo G; Rossi GB
IMMUNOPATHOLOGIC DISEASES: Diagnostic Immunopathology. Colvin RB et al, eds. New York, Raven, p. 41-197, 1988.. Unique Identifier : AIDSLINE ICDB/90658754; Anonymous; No affiliation given; Most of the major forms of glomerulonephritis are characterized by conspicuous glomerular accumulation of immunoglobulins, complement components, or both, often in distinctive patterns or with predominance of a particular immunoglobulin class. The use of immunofluorescence in the diagnosis of the following renal disea
TUMOR MARKERS IN DIAGNOSIS AND SCREENING: Int Congr Ser; 807:3-16 1989. Unique Identifier : AIDSLINE ICDB/90657991; Schwartz MK; Dept. of Clinical Chemistry, Memorial Sloan-Kettering Cancer; Center, 1275 York Ave., New York, NY 10021; A wide variety of substances can be identified as tumor markers. These include tumor-associated antigens, enzymes, specific proteins, and metabolites. Tumor markers employed in screening for cancer in general and in diagnosis and management of germ cell tumors and ovarian, colorectal, lung, breast, and prostate cancer
AIDS: AN EXPLANATION FOR ITS OCCURRENCE AMONG HOMOSEXUAL MEN: Aids and Infections of Homosexual Men. Second Edition. Ma P and Armstrong D, eds. Boston, Butterworths, p. 449-70 1989.. Unique Identifier : AIDSLINE ICDB/90659654; Sonnabend JA; Dept. of Pediatrics, St. Luke's-Roosevelt Medical Center, New; York, NY; A model is presented to explain the pathogenesis of AIDS in homosexual men; it does not require the participation of HIV or any other novel agent. The essential element of the model is that AIDS pathogenesis is an interactive, multifactorial process resulting from repeated exposures, particularly rectally, to large in
EPSTEIN-BARR VIRUS AND ACQUIRED IMMUNODEFICIENCY SYNDROME: Adv Viral Oncol; 8:203-17 1989. Unique Identifier : AIDSLINE ICDB/90659838; Ernberg I; Dept. of Tumor Biology, Karolinska Inst., Stockholm, Sweden; Next to T-helper lymphocytes, the Epstein-Barr virus (EBV)-carrying lymphoblasts were the second cell type shown to support HIV replication. The appearance of the oral hairy leukoplakias (OHLs), which can support EBV replication, is considered a bad prognostic sign in otherwise symptom-free HIV carriers. Approx one-fo
RECOMBINANT ANTIGENS AS DIAGNOSTIC AND SCREENING REAGENTS FOR HIV INFECTIONS: EXPERIENCE WITH A COMMERCIAL TEST: HIV Detection by Genetic Engineering Methods. Luciw PA and Steimer KS, eds. New York, Marcel Dekker, p. 77-98, 1989.. Unique Identifier : AIDSLINE ICDB/90660256; Decker RH; Dawson GJ; Abbott Laboratories, North Chicago, IL; Specificity and sensitivity of antibody-capture ELISAs are influenced significantly by the character and purity of the target antigens. Viral antigens generated by recombinant techniques appear to offer several advantages in new immunoassays for anti-HIV. Since most patients with AIDS and other HIV-infected subjects h
OPTIMAL USE OF DIAGNOSTIC LABORATORIES FOR EVALUATION OF PATIENTS WITH THE ACQUIRED IMMUNODEFICIENCY SYNDROME: Infect Dis Ther; 3:401-35 1989. Unique Identifier : AIDSLINE ICDB/90665456; Ng VL; Hadley WK; Dept. of Lab. Medicine, Univ. of California, San Francisco, CA; The diagnosis of AIDS depends on laboratory identification of the opportunistic infecting organism(s) as well as on the detection of HIV antibodies, the presence of which are an essential part of an AIDS diagnosis in a patient infected with a less-common opportunistic pathogen. This demand for the services of patholog
THE BIOLOGY OF HUMAN IMMUNODEFICIENCY VIRUSES: The Epidemiology of AIDS: Expression, Occurrence, and Control of Human Immunodeficiency Virus Type 1 Infection. Kaslow RA and Francis DP, eds. New York, Oxford University Press, p. 3-17, 1989.. Unique Identifier : AIDSLINE ICDB/90668108; Fultz P; Dept. of Pathology, Sch. of Medicine, Emory Univ., Atlanta, GA; 30322; The genomes of several isolates of HIV from different areas of the world have been sequenced, proteins encoded in the viral gene have been identified, and their individual functions have been studied. Epidemiologic data and clinical observations on immunologic abnormalities in AIDS patients helped to identify the prim
THE IMMUNE SYSTEM: CLINICAL MANIFESTATIONS: The Epidemiology of AIDS: Expression, Occurrence, and Control of Human Immunodeficiency Virus Type 1 Infection. Kaslow RA and Francis DP, eds. New York, Oxford University Press, p. 48-67, 1989.. Unique Identifier : AIDSLINE ICDB/90668111; Rogers P; Masur H; Presbyterian Univ. Hosp., DeSoto at O'Hara St., Rm. 1304,; Pittsburgh, PA 15213; Since the isolation of HIV-1 and the development of accurate serologic tests, clinical studies have demonstrated that HIV-1 infection is associated with a much broader spectrum of clinical manifestations than those included in the Centers for Disease Control s original definition of AIDS. Clinical manifestations of HI
ANTIVIRAL AGENTS AND VIRAL DISEASES OF MAN. THIRD EDITION: Antiviral Agents and Viral Diseases of Man. Third Edition. Galasso GJ et al, eds. New York, Raven, 786 p., 1990.. Unique Identifier : AIDSLINE ICDB/91676214; Anonymous; No affiliation given; As late as 1979, antiviral agents were somewhat of a novelty in clinical medicine. Their systematic discovery and development had not progressed much beyond research applications. Because of the research efforts stimulated in response to the AIDS epidemic, new antiviral agents have been developed recently. These inclu
HIV AND OTHER HUMAN RETROVIRUSES: Antiviral Agents and Viral Diseases in Man. Third Edition. Galasso GJ et al, eds. New York, Raven, p. 581-646, 1990.. Unique Identifier : AIDSLINE ICDB/91676216; Richman DD; Dept. of Pathology, Univ. of California at San Diego, San Diego,; CA 92161; The isolation and characterization of HIV, the human retrovirus that is the etiologic agent of AIDS, has become the most intensively investigated subject in the history of medicine. The Retroviridae consist of three subfamilies, the Oncovirinae, the Spumavirinae, and the Lentivirinae. The Oncovirinae consist primarily
EPSTEIN-BARR VIRUS AND HUMAN HERPESVIRUS TYPE 6: Antiviral Agents and Viral Diseases in Man. Third Edition. Galasso GJ et al, eds. New York, Raven, p. 647-68, 1990.. Unique Identifier : AIDSLINE ICDB/91676217; Straus SE; Medical Virology Section, Lab. of Clinical Investigation, Natl.; Inst. of Allergy and Infectious Diseases, Bethesda, MD 20892; All human herpesviruses infect and replicate within lymphocytes to some extent, but Epstein-Barr virus (EBV) and human herpesvirus type 6 (HHV-6) do so efficiently. The pathogenesis and host responses to infection with EBV (and perhaps with HHV-6) depend more on lymphocytic infection than is evident with other human h
BIOLOGY OF FELINE RETROVIRUSES: Retrovirus Biology and Human Disease. Gallo RC and Wong-Staal F, eds. New York, Marcel Dekker, p. 33-86, 1990.. Unique Identifier : AIDSLINE ICDB/91676220; Hardy WD Jr; Memorial Sloan-Kettering Cancer Center, New York, NY; There are three subfamilies of retroviruses: (1) Oncovirinae, which include such cancer-inducing viruses as the feline leukemia viruses (FeLVs), feline sarcoma viruses (FeSVs), avian leukosis viruses, and murine leukemia viruses; (2) Lentivirinae, which include HIV-1 and HIV-2; and (3) Spumavirinae, which infect many
GROWTH FACTORS AND RECEPTORS: Molecular Genetics in Cancer Diagnosis. Cossman J, ed. New York, Elsevier, p. 49-72, 1990.. Unique Identifier : AIDSLINE ICDB/91676245; Leonard WJ; Sharon M; Natl. Inst. of Child Health and Human Development, Bethesda, MD; In contrast to the carefully regulated growth control of normal diploid cells, transformed or malignant cells can grow in the absence of exogenous growth factors. The identification of oncogenes and the recognition that some of these oncogenes encode protein with significant homology to cellular growth factor receptor
HUMAN T-CELL LEUKEMIA VIRUS, TYPE 1, AND HUMAN LEUKEMIA AND LYMPHOMA: Molecular Genetics in Cancer Diagnosis. Cossman J, ed. New York, Elsevier, p. 163-78, 1990.. Unique Identifier : AIDSLINE ICDB/91676250; Reitz MS; Lab. of Tumor Cell Biology, NCI, Bethesda, MD; Human T-cell leukemia virus, type 1 (HTLV-1), was the first human retrovirus discovered. It is the etiologic agent of adult T-cell leukemia/lymphoma (ATL), a distinct form of leukemia/lymphoma recognized first in Japan . Reagents from HTLV-1 have aided in the diagnosis of this diseas
LYMPHOID PROLIFERATIONS ASSOCIATED WITH THE ACQUIRED IMMUNODEFICIENCY SYNDROME: Molecular Genetics in Cancer Diagnosis. Cossman J, ed. New York, Elsevier, p. 249-72, 1990.. Unique Identifier : AIDSLINE ICDB/91676255; Knowles DM; Dalla-Favera R; Coll. of Physicians and Surgeons of Columbia Univ., New York, NY; Malignant lymphomas occurring in association with AIDS are a significant and challenging clinical problem, primarily because of their distinctive clinicopathologic features. AIDS-associated lymphoid neoplasia was investigated in New York City. Medical records were screened to identify all patients (pts) seen between J
ERTHYROPOIETIN THERAPY FOR THE ANEMIA ASSOCIATED WITH AIDS AND AIDS THERAPY AND CANCER: Erythropoietin in Clinical Applications: An International Perspective. Garnick MB, ed. New York, Marcel Dekker, p. 301-24, 1990.. Unique Identifier : AIDSLINE ICDB/91676844; Danna RP; Rudnick SA; Abels RI; The R.W. Johnson Pharmaceutical Res. Inst., Raritan, NJ; AIDS, like other long-term illnesses such as chronic infections, inflammatory diseases, and cancer, frequently is associated with hypoplastic anemia. Often this anemia resembles the anemia of chronic disease. Therapeutic use of recombinant human erythropoietin (rHuEPO) in anemic patients (pts) seeks to replace or augm
INTERACTION OF HIV WITH ITS CELLULAR RECEPTOR, CD4: PROSPECTS FOR IMMUNOLOGIC INTERVENTION: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 63-92, 1990.. Unique Identifier : AIDSLINE ICDB/91676942; Orloff GM; McDougal JS; Center for Infectious Diseases, Centers for Disease Control,; Atlanta, GA; Because the initial interaction between HIV and host cells involves binding of the viral outer envelope glycoprotein gp120 to the CD4 molecule, much attention has been focused on the envelope and its binding site. Definition of the molecular nature of the CD4 receptor-HIV envelope interaction is not only a key to unde
STRUCTURE AND FUNCTION OF THE HIV ENVELOPE: IMPLICATIONS FOR VACCINE DEVELOPMENT: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 93-106, 1990.. Unique Identifier : AIDSLINE ICDB/91676943; Sodroski J; Kowalski M; Haseltine W; Lab. of Human Retrovirology, Dana-Farber Cancer Inst., Boston, MA; The HIV is surrounded by an envelope composed of a lipid bilayer, derived from the host cell as the virus buds, and envelope glycoproteins encoded by the virus. Cleavage of a precursor 160-kD glycoprotein by a cellular protease yields the exterior glycoprotein, gp120, and the transmembrane glycoprotein, gp41. Both pro
CONSERVED AND DIVERGENT FEATURES OF HUMAN AND SIMIAN IMMUNODEFICIENCY VIRUSES: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 107-19, 1990.. Unique Identifier : AIDSLINE ICDB/91676944; Franchini G; Wong-Staal F; Lab. of Tumor Cell Biology, NCI, Bethesda, MD; The simian immunodeficiency virus (SIVmac) has been isolated from captive rhesus macaques with clinical signs of immunodeficiency and chronic degenerative brain disorders resembling human AIDS. Experimental inoculation of SIV in macaques induces immunodeficiency at high frequency, providing an excellent animal model t
GENETIC VARIABILITY IN HUMAN IMMUNODEFICIENCY VIRUSES: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 121-35, 1990.. Unique Identifier : AIDSLINE ICDB/91676945; Hahn BH; Shaw GM; Dept. of Medicine, Univ. of Alabama, Birmingham, AL; Genetic diversity is a hallmark of HIV and simian immunodeficiency virus (SIV). Current knowledge of HIV/SIV genetic variation and how it is generated is reviewed, including polymorphism of HIV isolates and the rapidly evolving mixture of genetic variants, genotypic variation as a result of errors in HIV reverse trans
T-CELL IMMUNITY AND VACCINE ENGINEERING: APPLICATION TO THE AIDS VIRUS: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 139-56, 1990.. Unique Identifier : AIDSLINE ICDB/91676946; Cease KB; Berzofsky JA; Dept. of Internal Medicine, Univ. of Michigan Medical Sch., Ann; Arbor, MI; Protective immunity against the AIDS virus is the goal of all current AIDS vaccine research. However, such an immunity has not been demonstrated in man under any circumstances. The development of a safe and effective vaccine for AIDS clearly is one of the most difficult vaccine challenges in history. The role of T-cel
ANTI-HIV-1 ADCC: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 157-77, 1990.. Unique Identifier : AIDSLINE ICDB/91676947; Lyerly HK; Tyler DS; Nastala CL; Weinhold KJ; Dept. of Surgery, Duke Univ. Medical Center, Durham, NC; Several virus-specific cytotoxic activities are capable of destroying HIV-1-infected cells. One of these--antibody-dependent cellular cytotoxicity (ADCC)--is reviewed with respect to HIV-1-specific protective and pathogenic responses, with particular emphasis on recent work by the authors. Topics include target antige
CYTOTOXIC T-LYMPHOCYTE RESPONSES TO HIV-1 IN INFECTED INDIVIDUALS: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 179-94, 1990.. Unique Identifier : AIDSLINE ICDB/91676948; Walker BD; Dept. of Medicine, Harvard Medical Sch., Boston, MA; AIDS is associated with a persistent viremia, with virus readily cultured from the peripheral blood of infected individuals. Despite this, most individuals remain asymptomatic for years after infection, suggesting that a virus-specific host immune response might play a role in keeping these people healthy in the face
BIOCHEMICAL AND IMMUNOLOGIC CHARACTERIZATIONS OF HIV ENVELOPE GLYCOPROTEINS EXPRESSED BY RECOMBINANT VACCINIA VIRUS: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 197-217, 1990.. Unique Identifier : AIDSLINE ICDB/91676949; Hu SL; Oncogen, Seattle, WA; Vaccinia virus has been used successfully for centuries as the vaccine against smallpox. Recombinant vaccinia viruses have been constructed to express the envelope glycoproteins of HIV-1. Biochemical and immunologic characterization of these proteins is reviewed, and preliminary results of the evaluation of these reco
PROPHYLACTIC AND THERAPEUTIC IMMUNIZATION AGAINST AIDS: THEORETICAL CONSIDERATIONS UNDERLYING A MODIFIED NONINFECTIOUS WHOLE VIRUS APPROACH: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 265-75, 1990.. Unique Identifier : AIDSLINE ICDB/91676951; Salk J; Gersten MJ; The Salk Inst. for Biological Studies, San Diego, CA; Post-HIV infection events may be modulated by (1) prepriming the immune system by active immunization of HIV seronegatives, to respond anamnestically to subsequent HIV exposure, to prevent establishment of infection and/or disease or (2) through active and/or passive immunization of HIV seropositives, to prevent, arre
INDUCTION OF PROTECTIVE IMMUNITY AGAINST AN IMMUNOSUPPRESSIVE MOUSE RETROVIRUS: POSSIBLE PARALLELS WITH HIV: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 301-18, 1990.. Unique Identifier : AIDSLINE ICDB/91676952; Chesebro B; Rocky Mountain Lab., Natl. Inst. of Allergy and Infectious; Diseases, Hamilton, MT; Friend virus (FV) maintains a chronic persistent infection of many cell types in vivo. Effective protective immunity should be capable of recognizing both cell-free virions and infected cells. The FV mouse model may be appropriate for studying problems of development of protective immunity that might be relevant to HI
INFECTION OF NONHUMAN PRIMATES WITH HUMAN AND SIMIAN IMMUNODEFICIENCY VIRUSES: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 339-49, 1990.. Unique Identifier : AIDSLINE ICDB/91676953; Fultz PN; McClure HM; Anderson DC; Yerkes Regional Primate Res. Center, Emory Univ., Atlanta, GA; For an animal model to be useful in vaccine efficacy studies, it must meet the following requirements: (1) essentially all animals become infected following inoculation of virus; (2) infection can be detected easily by isolation of virus, which can be quantified; (3) seroconversion occurs; and (4) infection elicits di
RELEVANT ASPECTS OF HIV-RELATED VIRUSES TO VACCINE DEVELOPMENT: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 351-9, 1990.. Unique Identifier : AIDSLINE ICDB/91676954; Kanki PJ; Eichberg JW; Essex M; Harvard AIDS Inst., Harvard Univ., Boston, MA; Monkeys and apes have sometimes been the only animal species other than man infected with important human viruses such as polio, hepatitis B virus, measles, yellow fever, and Marburg virus. HIV-related viruses in primates are discussed in relation to their role in vaccine development. Topics include discovery of simia
USE OF SIMIAN IMMUNODEFICIENCY VIRUS FOR AIDS VACCINE RESEARCH: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 361-7, 1990.. Unique Identifier : AIDSLINE ICDB/91676955; Desrosiers RC; Li Y; Daniel MD; New England Regional Primate Res. Center, Southborough, MA; The development of most viral vaccines for use in humans relied heavily on animal models for safety and efficacy testing. It seems likely that animal model testing will play an important role in the development of the best possible AIDS vaccines. The role of simian immunodeficiency viruses (SIVs) in AIDS vaccine resea
A SPECIFIC ANTIBODY ACTIVITY THAT NEUTRALIZES HIV INFECTIVITY IN VIVO: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 369-78, 1990.. Unique Identifier : AIDSLINE ICDB/91676956; Emini EA; Dept. of Virus and Cell Biology, Merck Sharp and Dohme Res.; Laboratories, West Point, PA; The task of developing a vaccine against HIV is complicated by a lack of fundamental knowledge concerning vaccine-relevant immune responses against the virus. The significance of the general anti-HIV responses elicited during persistent HIV infection remains a question. In vitro HIV-neutralizing antibody and favorable
RECOMBINANT-DERIVED HEPATITIS B VACCINE: A PARADIGM FOR OTHER SUBUNIT VACCINES: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 381-407, 1990.. Unique Identifier : AIDSLINE ICDB/91676957; Ellis RW; Dept. of Cellular and Molecular Biology, Merck Sharp and Dohme; Res. Lab., West Point, PA; The yeast-derived hepatitis B vaccine is the first recombinant-derived vaccine approved for human use. It serves as a paradigm for the development of future recombinant subunit vaccines. The molecular biology and expression system, preclinical biological analysis of vaccine antigen, and clinical testing of this virus
CLINICAL TRIALS OF AIDS VACCINES: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 425-38, 1990.. Unique Identifier : AIDSLINE ICDB/91676958; Koff WC; Wescott SL; Hoth DF; Vaccine Res. and Development Branch, Natl. Inst. of Allergy and; Infectious Diseases, Bethesda, MD; Concomitant with the efforts in preclinical vaccine development, preliminary clinical trials of experimental AIDS vaccines have been initiated in the absence of demonstrable protective immunity in animal models. Major issues associated with clinical trials of AIDS vaccines are discussed, including design of Phase 1, 2
ONCOGENESIS: ONCOGENES IN SIGNAL TRANSDUCTION AND CELL PROLIFERATION: Adv Applied Biotechnol Ser; 6:1-343 1990. Unique Identifier : AIDSLINE ICDB/91677155; Anonymous; No affiliation given; So far about 55 oncogenes have been identified. The products of many of these genes have been identified in cells and great efforts are now being made to understand the biology and role of these oncogene products in cell development and growth. Just how these gene products interact and affect the initiation of cell gr
DOMINANT NEGATIVE MUTATIONS OF RAS ONCOGENES: Adv Applied Biotechnol Ser; 6:77-91 1990. Unique Identifier : AIDSLINE ICDB/91677161; Shih TY; Ogiso Y; Gutierrez L; Wrathall LS; Hwang YW; Lab. of Molecular Oncology, NCI, Frederick, MD 21701-1013; A general approach to elucidate the cellular function of a molecularly cloned regulatory gene is to construct dominant negative mutants capable of functional inactivation of the resident wild type gene when introduced into a recipient cell. Regulatory molecules in general possess at least two functional domains for re
FACTORS INVOLVED IN IMMUNE MODULATION OF BOVINE LEUKEMIA VIRUS INFECTION: Diss Abstr Int [B]; 51(9):4223 1991. Unique Identifier : AIDSLINE ICDB/91676122; Pool WV; Louisiana State Univ. and Agricultural and Mechanical Coll.; Bovine leukemia virus (BLV) is an exogenous type-C retrovirus which can induce an aleukemic (AL) stage, a persistent lymphocytotic (PL) stage, or a lymphosarcomatous stage in infected animals. After experimental infection with BLV, whole blood and serum samples were obtained from 12 holstein heifers for 103 wk and use
BOVINE LEUKEMIA VIRUS (BLV): A MODEL VIRUS FOR THE HUMAN T LYMPHOCYTOTROPHIC RETROVIRUSES: Diss Abstr Int [B]; 51(9):4222 1991. Unique Identifier : AIDSLINE ICDB/91676137; Johnson R; Michigan State Univ.; The objective of these studies was to investigate the potential of BLV infection in sheep as a model system for studying the biology of leukemogenic retroviruses of animals and humans. A prospective study of the serologic, hematologic, and histologic changes of sheep infected with BLV was done. Whole blood from a BLV
KAPOSI'S SARCOMA: PATHOGENESIS, ULTRASTRUCTURE AND EPIDEMIOLOGY: Diss Abstr Int [C]; 52(1):99 1991. Unique Identifier : AIDSLINE ICDB/91676166; Dictor MR; Lunds Universitet, Sweden; Kaposi s sarcoma is an abnormal proliferation of lymphatic and venous endothelium beginning at the capillary level. Coupling between lymphatics and veins is suggested histologically by glomeruloid structures at the capillary level and radial venolymphatics during orthograde progression of the lesion along veins. Abnor
HUMAN PAPILLOMAVIRUS AND HUMAN IMMUNODEFICIENCY VIRUS INFECTION IN RELATION TO CERVICAL CANCER: STUDIES AND OBSERVATIONS OF BASIC CLINICAL AND EPIDEMIOLOGICAL ASPECTS OF CANCER OF THE CERVIX WITH SPECIAL REFERENCE TO KENYA, AFRICA: Diss Abstr Int [C]; 52(1):91 1991. Unique Identifier : AIDSLINE ICDB/91676168; Rogo KO; Umea Universitet, Sweden; Carcinoma of the cervix is the most common female malignancy in Kenya . No screening program has been started in the country and, with only one public hospital with a high-energy radiation facility, few women receive optimal care. Little is known about the epidemiology of the disease
ORGAN DISTRIBUTION OF FOLATES IN TRANSGENIC MICE WITH TUMORS AND IN NONTRANSGENIC LITTERMATES (MEETING ABSTRACT): FASEB J; 5(6):A1639 1991. Unique Identifier : AIDSLINE ICDB/91676495; Bills ND; Hinrichs SH; Clifford AJ; Dept. of Nutrition, Univ. of California, Davis, CA 95616; Transgenic mice (TM) with known predisposition to develop neoplasia (Science 237:1340-3, 1987) were fed a cereal-based diet since weaning and used to evaluate the effects of cancer on distribution of total body folate among major host organs and tumors. TM have germ line transmission of a transgene incorporating the l
IN VITRO EFFECT OF ANTISENSE OLIGOMERS ON INFECTION WITH AND PRODUCTION OF MOUSE MAMMARY TUMOR VIRUS (MMTV) (MEETING ABSTRACT): FASEB J; 5(6):A1705 1991. Unique Identifier : AIDSLINE ICDB/91676500; Rucker P; Myers WL; Southern Illinois Univ. Sch. of Medicine, Springfield, IL; 62794-9230; The use of antisense oligomers has been shown to be effective in inhibiting replication of another retrovirus, human immunodeficiency virus (HIV). The purpose of this study was to investigate antisense oligomers which may be useful in inhibiting MMTV infection and/or replication. We have synthesized five 20-mer oligom
ENHANCEMENT OF LOW-DOSE MELPHALAN-INDUCED TUMOR ERADICATING IMMUNITY BY DEPLETION OF CD4+ T-CELLS (MEETING ABSTRACT): FASEB J; 5(6):A1773 1991. Unique Identifier : AIDSLINE ICDB/91676525; Weiskirch LM; Mokyr MB; Univ. of Illinois, Chicago, IL 60612; We have previously shown that a low dose (2.5 mg/kg) of melphalan (L-phenylalanine mustard; L-PAM) is curative for approx 90% of BALB/c mice bearing a large sc MOPC-315 tumor and extensive metastases if CD8+ T-cell-dependent antitumor immunity aids in tumor eradication. The CD8+ T-cells were shown to eradicate at leas
CONCEPTS OF CANCER BIOLOGY: TUMOR IMMUNOLOGY: Comprehensive Textbook of Oncology. Second Edition. Moossa AR et al, eds. Baltimore, Williams and Wilkins, p. 101-19, 1991.. Unique Identifier : AIDSLINE ICDB/91676538; Anonymous; No affiliation given; There is justifiable optimism for a renewed intensification of efforts to translate modern immunology and molecular technology into tumor immunotherapeutic protocols. Activating the immune system can cause a response against quiescent tumor cells and lymphocytes can retain long-term memory for tumor-specific reactivit
APPROACHES TO SPECIFIC NEOPLASMS: ACQUIRED IMMUNE DEFICIENCY SYNDROME: Comprehensive Textbook of Oncology. Second Edition. Moossa AR et al, eds. Baltimore, Williams and Wilkins, p. 1313-36, 1991.. Unique Identifier : AIDSLINE ICDB/91676558; Anonymous; No affiliation given; Since 1981, when the first cases of AIDS were reported by the Centers for Disease Control, much has been learned about this disorder. Dramatic scientific achievements have occurred nearly every year since AIDS was first defined; these include discovery of the etiologic agent (HIV), development of a blood test for anti
APPROACHES TO SPECIFIC NEOPLASMS: CHILDHOOD CANCER: Comprehensive Textbook of Oncology. Second Edition. Moossa AR et al, eds. Baltimore, Williams and Wilkins, p. 1469-614, 1991.. Unique Identifier : AIDSLINE ICDB/91676563; Anonymous; No affiliation given; Only 6% of cancer deaths occur under 45 yr of age and less than 1% occur under 14 yr of age. Impressive progress has been made in the treatment of most childhood malignancies, but it is estimated that of the approx 6000 children under 14 yr of age who develop cancer each year in the United
AIDS VACCINE RESEARCH AND CLINICAL TRIALS: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, 480 p., 1990.. Unique Identifier : AIDSLINE ICDB/91676940; Anonymous; No affiliation given; Recently, impressive strides have been made in the development of vaccines against complex viruses such as hepatitis B. However, HIV has several characteristics that make it a more formidable challenge: it is a retrovirus; it varies the amino-acid sequence of the envelope and other immune targets; readily available an
FEATURES OF THE HIV ENVELOPE AND DEVELOPMENT OF A SUBUNIT VACCINE: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 3-61, 1990.. Unique Identifier : AIDSLINE ICDB/91676941; Putney SD; Javaherian K; Rusche J; Matthews T; Bolognesi DP; Repligen Corporation, Cambridge, MA; A major question in the development of an HIV vaccine is whether the body can mount an immune response capable of preventing initial infection and, if so, whether cell-mediated or antibody-mediated immunity is required. The characteristics of the HIV envelope, mapping of determinants that are targets for immune respon
CD4-GP120 INTERACTION: IDIOTYPE MIMICRY AS PUTATIVE VACCINES AND THERAPEUTICS AGAINST HIV INFECTION: AIDS Vaccine Research and Clinical Trials. Putney SD and Bolognesi DP, eds. New York, Marcel Dekker, p. 241-64, 1990.. Unique Identifier : AIDSLINE ICDB/91676950; Kennedy RC; Chanh TC; Maino VC; Warner NL; Koff WC; Dept. of Virology, and Immunology, Southwest Foundation for; Biomedical Res., San Antonio, TX; The network theory of immune regulation was proposed to explain how idiotype anti-idiotype interactions may be involved in regulating the immune response to an antigen. An idiotype (Id) defines the variable (V) region of the antibody molecule and often serves as a V region phenotypic marker. Even within a single antib
Risk reduction and stabilization of HIV seroprevalence among drug injectors in New York City and Bangkok, Thailand.: Int Conf AIDS. 1991 Jun 16-21;7(1):20 (abstract no. M.C.1). Unique Identifier : AIDSLINE ICA7/1000191; DesJarlais DC; Choopanya K; Wenston J; Vanichseni S; Sotheran JL; Plangsringarm K; Beth Israel Medical Center, New York, New York, USA; OBJECTIVE: To compare long term and recent trends in HIV seroprevalence and AIDS risk reduction among drug injectors in New York City (NY) and Bangkok (B). METHODS: Data on seroprevalence and risk behavior have been collected by the participating researchers since early in the epidemic in both cities. New subjects wer
The impact of STD control and condom promotion on the incidence of HIV in Kinshasa prostitutes.: Int Conf AIDS. 1991 Jun 16-21;7(1):20 (abstract no. M.C.2). Unique Identifier : AIDSLINE ICA7/1000291; Mulivanda T; Manoka AT; Nzila N; Way W; St Louis M; Piot P; Laga M; Projet SIDA, Kinshasa, Zaire; OBJECTIVE: To determine the impact of an intervention programme, including condom distribution and treatment for STD, on the incidence of HIV and other STD among female prostitutes in Kinshasa. METHODS: Prostitutes were asked to return monthly to a women s clinic where they were interviewed about their sexual exposure
Perinatal HIV-1 transmission in Nairobi, Kenya: 5 year follow-up.: Int Conf AIDS. 1991 Jun 16-21;7(1):20 (abstract no. M.C.3). Unique Identifier : AIDSLINE ICA7/1000391; Datta P; Embree J; Ndinya-Achola JO; Kreiss J; Plummer FA; University of Nairobi, Nairobi, Kenya; OBJECTIVE: To determine the perinatal HIV-1 transmission rate and evaluate the associated child morbidity and mortality. METHODS: Prospective cohort study of infants born to HIV-1 seropositive mothers (case children) and those born to HIV-1 seronegative mothers (control children). HIV infection in a case child was def
Assessment of incidence and risk factors for male to female HIV transmission.: Int Conf AIDS. 1991 Jun 16-21;7(1):20 (abstract no. M.C.4). Unique Identifier : AIDSLINE ICA7/1000491; Musicco M; Saracco A; Nicolosi A; Gervasoni C; Arici C; Angarano G; Vaccher E; Quirino T; Sinicco A; Turbessi G; et al; National Research Council of Italy, ITBA - Department of; Epidemiology, Milan, Italy; OBJECTIVE: This cross-sectional and prospective study of 524 women who were steady partners of HIV-infected men was carried out in order to assess the incidence and risk factors of man-to-woman sexual HIV. METHODS: The survey considered women, with no risk factors other than that of exposure to their sexual partner, r
Small regions of the env and tat genes control cellular tropism, cytopathology and replicative properties of HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):21 (abstract no. M.A.5). Unique Identifier : AIDSLINE ICA7/1000591; Cheng-Mayer C; Shioda T; Levy JA; Cancer Research Institute, University of California, School of; Medicine, San Francisco, California 94143-0128; OBJECTIVE: To identify genetic regions of HIV-1 that control the pathogenic properties of the virus. METHODS: Recombinant viruses were generated between two sequential isolates (HIV-1SF2, HIV-1SF13) that are highly related at the genomic level and yet display distinct in vitro biological properties. RESULTS: The seque
Factors governing HIV-1 persistence in vivo.: Int Conf AIDS. 1991 Jun 16-21;7(1):21 (abstract no. M.A.6). Unique Identifier : AIDSLINE ICA7/1000691; Stevenson M; Bukrinsky MI; Stanwick TL; Dempsey MP; Department of Pathology and Microbiology, University of Nebraska; Medical Center, Omaha, NE USA; The underlying basis for HIV-1 persistence and latency and the nature of the virus reservoir in the course of AIDS remains unresolved. The large number of cells in infected individuals which harbor proviral DNA is difficult to reconcile with low numbers of productively infected cells suggesting a restricted reservoir
Mutation in T cell epitopes of HIV gag provides a mechanism for escape from immune control.: Int Conf AIDS. 1991 Jun 16-21;7(1):21 (abstract no. M.A.7). Unique Identifier : AIDSLINE ICA7/1000791; Phillips R; Rowland-Jones S; Gotch F; Nixon DF; Ogunlesi A; Edwards J; McMichael AJ; Institute of Molecular Medicine, John Radcliffe Hospital, Oxford,; UK; OBJECTIVE: In longitudinal studies of HIV positive haemophiliacs, three distinct cytotoxic T cell (CTL) epitopes have been identified in Gag. To investigate the mechanism of fluctuation in CTL recognition of these epitopes and to search for escape mutants, we have determined the nucleotide sequence of HLA-B8 restricte
Genomic diversity and antigenic variation of the V3 domain as determining factors of HIV-1 pathogenesis.: Int Conf AIDS. 1991 Jun 16-21;7(1):21 (abstract no. M.A.8). Unique Identifier : AIDSLINE ICA7/1000891; Goudsmit J; Wolfs T; Kuiken C; Zwart G; Tersmette T; Human Retrovirus Laboratory, University of Amsterdam, Amsterdam,; the Netherlands; OBJECTIVE: To determine the relationship between the diversity of the viral population, the emergence of HIV-1 variants escaping V3 specific antibody, the switch in virus phenotype from non-syncytium inducing (NSI) to syncytium inducing (SI), and progression to AIDS. METHODS: Sequential serum samples were used as sour
Role of N-linked glycans in the folding of HIV-1 envelope glycoproteins.: Int Conf AIDS. 1991 Jun 16-21;7(1):24 (abstract no. M.A.9). Unique Identifier : AIDSLINE ICA7/1000991; Fenouillet E; Gluckman JC; CERVI, Hopital Pitie-Salpetriere, Paris - France; OBJECTIVE: Our recent results indicate that carbohydrates (CHO) present on mature env glycoproteins (gp) do not play a major role for HIV-1 infectivity (J. Exp. Med. 1989, March; J. Virol. 1990, June). However, sugar analogs like 1 deoxynojirimycin (dNM) that interfere with the glycosylation process of nascent gp160 m
Quantitative analysis of the binding kinetics and thermodynamics of gp120-CD4 interaction reveals a three step process and a new mode of allosteric inhibition of gp120 binding.: Int Conf AIDS. 1991 Jun 16-21;7(1):24 (abstract no. M.A.10). Unique Identifier : AIDSLINE ICA7/1001091; Repke H; Shin J; Haseltine WA; Division of Human Retrovirology, Dana-Farber Cancer Institute and; Harvard Medical School, Boston, USA; OBJECTIVE: To understand the conformational pertubations of CD4 and gp120 during the binding process and to find alternative ways of inhibiting binding, syncytia formation and infectivity. METHODS: gp120 (HIV-1 and HIV-2), soluble and membrane bound CD4/CD4-mutants and immunoadhesins were used to study association/dis
Recovery of C8166 cells from HIV infection is due to reversibility of provirus integration.: Int Conf AIDS. 1991 Jun 16-21;7(1):24 (abstract no. M.A.11). Unique Identifier : AIDSLINE ICA7/1001191; Benedetto A; Di Caro A; Elia G; Garbuglia AR; Delfini C; Center Virology, S. Camillo Hosp; Rome; OBJECTIVE: Investigation of CD4 receptor, citopathic effect, viral expression, and integration in C8166 cells and in derived clones, surviving to HIV (HTLV-IIIB) infection. METHODS AND RESULTS: C8166 cells show maximal susceptibility to HIV, resulting in a near-synchronous infection cycle and cell death, within 4-5 da
Function of the HIV-1 envelope carboxyl terminus.: Int Conf AIDS. 1991 Jun 16-21;7(1):24 (abstract no. M.A.12). Unique Identifier : AIDSLINE ICA7/1001291; Gabuzda D; Lever A; Haseltine W; Sodroski J; Dana-Farber Cancer Institute and Harvard Medical School, Boston,; MA, USA; OBJECTIVE: The HIV-1 envelope glycoprotein carboxyl terminus contains sequences which are essential for efficient viral replication. To determine the function of this domain in the virus life cycle, the effect of mutations in the carboxy terminal 160 amino acids of gp41 on viral replication, envelope glycoprotein synt
Metabolic and structural effects of the interaction of HIV with PBL and lymphoblastoid cells can be monitored with 1H NMR.: Int Conf AIDS. 1991 Jun 16-21;7(1):25 (abstract no. M.A.13). Unique Identifier : AIDSLINE ICA7/1001391; Federico M; Maggiorella MT; Sulli N; Guidoni L; Luciani AM; Rosi A; Viti V; Verani P; Rossi GB; Lab.di Fisica e INFN sez. Sanita; OBJECTIVE: To elucidate changes induced in cell membrane organization and in cellular lipid metabolism following HIV infection of susceptible cells, taking advantage of NMR spectroscopy that can be performed on intact viable cells. METHODS: PHA stimulated human peripheral blood lymphocytes (PBL), like other proliferat
Noncompetitive inactivation of HIV-1 by rCD4 and CD4-Ig.: Int Conf AIDS. 1991 Jun 16-21;7(1):25 (abstract no. M.A.14). Unique Identifier : AIDSLINE ICA7/1001491; Berkower I; Murphy D; Smith GE; CBER FDA, Bethesda, MD, USA; OBJECTIVE: To determine how many recombinant CD4 (rCD4) or CD4-immunoglobulin hybrid molecules must bind each virus to cause inactivation. METHODS: HIV-1 inactivation by rCD4 and CD4-Ig were measured in a sensitive plaque-forming assay, which gave a quantitative measure of virus survival as a fraction of input virus.
HIV envelope contains a structural MHC class II homology consistent with an alloepitope.: Int Conf AIDS. 1991 Jun 16-21;7(1):25 (abstract no. M.A.15). Unique Identifier : AIDSLINE ICA7/1001591; Dalgleish A; Habeshaw J; Manca F; Hounsell E; MRC Clinical Research Centre Harrow HA1 3UJ UK; OBJECTIVE: 1) To show that gp120 contains an alloepitope which would be available to interact with the T cell receptor (TCR), and to explore if this interaction is with a specific V-alpha/beta region of the TCR. 2) To determine if T cell responses against HIV are T cell restricted at the V-alpha/beta level. METHODS: M
Molecular mimicry between HIV-1 gp41 and HLA class II: correlation between the generation of crossreactive antibodies, T cell function, and disease progression in infected individuals.: Int Conf AIDS. 1991 Jun 16-21;7(1):25 (abstract no. M.A.16). Unique Identifier : AIDSLINE ICA7/1001691; Golding H; Clerici M; Mann D; Shearer GM; Blackburn R; Division of Virology, FDA, Bethesda, MD, USA; Two highly homologous sequences were found in conserved regions of HIV-1 gp41 (aa837-844), and human HLA class II beta chains (aa19-25). Early study demonstrated that 35% of HIV-1 infected individuals produce antibodies against the gp41 sequence which crossreact with HLA class II bearing cells (CRAb). Such CRAb could
Role of the LFA-1/ICAM-1/2 pathway of cell adhesion in HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):26 (abstract no. M.A.17). Unique Identifier : AIDSLINE ICA7/1001791; Butini L; Pantaleo G; Poli G; Dayton A; Fauci AS; LIR, NIAID, NIH, Bethesda, MD, USA; OBJECTIVE: To determine the role of the LFA-1/ICAM-1/2 pathway of cell adhesion in the process of HIV-mediated cell fusion and HIV spreading. METHODS: Jurkat cells were transfected with HXB2, a plasmid that contains a full length, infectious human T lymphotropic virus (HTLV)-IIIB. PHA-activated LFA-1+ and LFA-1(-) [ob
Research for the effect of gp120/CD4 interactions in vivo.: Int Conf AIDS. 1991 Jun 16-21;7(1):26 (abstract no. M.A.18). Unique Identifier : AIDSLINE ICA7/1001891; Amadori A; Zamarchi R; Veronese ML; DeSilvestro G; Salmaso L; Barelli A; Borri A; Chieco-Bianchi L; Institute of Oncology, Padova, ITALY; PREMISS: Much in vitro data indicate the effects of gp120/CD4 interactions, but no evidence that this phenomenon occurs in vivo has been provided so far. METHODS: PBL were analyzed cytofluorographically with different mAb against CD4; CD4 expression and proliferative response to anti-CD3 was studied before and after i
The role of IL-6 and TNF-alpha production by B-lymphocytes in HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):26 (abstract no. M.A.19). Unique Identifier : AIDSLINE ICA7/1001991; Rieckmann P; Poli G; Fox CH; Kehrl JH; Fauci AS; Laboratory of Immunoregulation, National Institute of Allergy and; Infectious Diseases, National Institutes of Health, Bethesda, MD,; USA; OBJECTIVE: To investigate the role of IL-6 and TNF-alpha produced by activated B cells in HIV infected individuals for their potential to support B cell activation and HIV expression. METHODS: B cells were isolated from the blood or lymph node, cultured in the presence or absence of polyclonal B cell activators or rec
Effect of in vitro infection with HIV-1 on the function of CD4+ antigen-specific human T cell clones.: Int Conf AIDS. 1991 Jun 16-21;7(1):26 (abstract no. M.A.20). Unique Identifier : AIDSLINE ICA7/1002091; Romagnani S; Macchia D; Parronchi P; Piccinni MP; Simonelli C; Ravina A; Mazzetti M; Santoni F; Maggi E; Istituto di Clinica Medica 3, Universita di Firenze (Italia); OBJECTIVE: To examine the alterations induced by HIV-1 infection on the function of helper T cells with particular regard to their involvement in the polyclonal B cell response. METHODS: In vitro infection with HIV-1 of PPD- or tetanus toxoid-specific CD4+ human T cell clones (TCC) derived from uninfected individuals.
Prevention of HIV-2 and SIVsm infection by passive immunization in cynomolgus monkeys.: Int Conf AIDS. 1991 Jun 16-21;7(1):27 (abstract no. M.A.21). Unique Identifier : AIDSLINE ICA7/1002191; Putkonen P; Thorstensson R; Albert J; Hild K; Biberfeld G; Norrby E; Dept. of Immunology, National Bact. Lab.; OBJECTIVE: To evaluate the efficacy of passive immunization against HIV-2 and SIVsm infection in cynomolgus monkeys (Macaca Fascicularis). METHODS: Sera with high antibody titers were heat treated and injected intravenously (iv) into naive animals six hours before iv challenge with live homologous cell-free virus. In
HIV2 ROD infection of macaques resulted in U3 deletion in vivo.: Int Conf AIDS. 1991 Jun 16-21;7(1):27 (abstract no. M.A.22). Unique Identifier : AIDSLINE ICA7/1002291; Nicol I; Boussin F; Vogt G; LeGrand R; Montagnier L; Dormont D; CEA, CRSSA, DSV/DPTE/SSA, Fontenay aux Roses, France; OBJECTIVES: AIDS-like clinical manifestations were observed in an HIV2-ROD infected macaque (33215). This isolate was reinoculated into macaques to obtain an animal model for AIDS disease. Here, we report the in vivo HIV2-ROD genomic variations in the monkey 33215 and the results of the second inoculation. METHODS: In
Generation of high amounts of replicative defective infectious SIV by transcomplementation of a mutated rev with HTLV-1 rex.: Int Conf AIDS. 1991 Jun 16-21;7(1):27 (abstract no. M.A.23). Unique Identifier : AIDSLINE ICA7/1002391; Krohn K; Hakkarainen K; Aavik E; Dewhurst S; Sadaie R; Mullins J; University of Tampere, Tampere, Finland; OBJECTIVE: Genetically engineered replication deficient viruses can theoretically be used as attenuated vaccines. In HIV and SIV, the rev protein is known to be necessary for expression of viral structural proteins and for the production of infectious virus. We have determined the ability of HTLV-1 rex protein to comp
Vaccination against SIV in macaques.: Int Conf AIDS. 1991 Jun 16-21;7(1):27 (abstract no. M.A.24). Unique Identifier : AIDSLINE ICA7/1002491; Mills KH; Stott EJ; Chan WL; Taffs LF; Cranage M; Greenaway P; Thiriart C; DeWilde M; Bruck C; Page M; et al; NIBSC, Potters Bar, Herts., UK; OBJECTIVE: To assess the ability of SIV vaccines to generate humoral and cell-mediated immunity and to protect against challenge in an SIV macaque model. METHODS: Macaques were immunized with 4 doses of inactivated whole virus or recombinant SIV proteins. Humoral immune responses were tested by ELISA, immunoblots, RIP
Mapping of neutralizing antibodies on the SIV env glycoprotein.: Int Conf AIDS. 1991 Jun 16-21;7(1):28 (abstract no. M.A.25). Unique Identifier : AIDSLINE ICA7/1002591; Collignon C; Thiriart C; Langedijk H; Meloen R; Desrosiers R; DeWilde M; Bruck C; SmithKline Beecham Biologicals, Rixensart, Belgium; Several monoclonal antibodies were derived from Balb/c mice immunized with vaccinia expressed SIV gp150. These reagents were characterized for reactivity to different forms of SIV envelope protein and to different SIV isolates using radioimmunoprecipitation and Western Blot (WB). Among these, 2 showed a neutralizing a
Changes in the transmembrane protein are associated with cytopathic effects of SIV.: Int Conf AIDS. 1991 Jun 16-21;7(1):28 (abstract no. M.A.26). Unique Identifier : AIDSLINE ICA7/1002691; LaBranche CC; Bugelski PJ; Hart TK; Hoxie JA; Univ. of Pennsylvania, Philadelphia, PA; OBJECTIVE: To identify the determinants of cytopathogenesis in a cytopathic variant of molecularly-derived SIVmac251. METHODS: SIVmac251 has been shown to infect SUP-T1 cells without killing, fusion, or CD4 down-modulation. A cytopathic variant of this virus arose spontaneously during long-term culture. The parental a
c-sis/PDGFB is transcribed in primary lymphocytes exposed to HTLV-1: a possible role for the PDGF autocrine circuit in the control of proliferation in infected cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):28 (abstract no. M.A.27). Unique Identifier : AIDSLINE ICA7/1002791; D'Onofrio C; Faraoni I; Macchi B; Bonmassar E; Dept. Exp. Med. Biochem. Sci., II University of Rome, Rome, Italy; OBJECTIVE: PDGF has been described not to be expressed in T lymphocytes under physiological conditions. More recently, PDGFB and its receptor have been found to be expressed in T cell lines infected with HTLV-I (D Onofrio et al., J. IFN Res.9(2s):S82, 1989; Goustin et al., Growth Factors 2:189, 1990). These data sugge
Comparative analysis of HTLV-I envelope sequences.: Int Conf AIDS. 1991 Jun 16-21;7(1):28 (abstract no. M.A.28). Unique Identifier : AIDSLINE ICA7/1002891; Calabro ML; Hoad JH; Matutes E; Catovsky D; Weiss RA; Schulz TF; Institute of Cancer Research, Chester Beatty Labs., London,; England; OBJECTIVE: Comparative analysis of HTLV-I envelope sequences derived from ATL cases of different countries was performed in order to evaluate their genetic distance from prototype sequences and to identify amino acid changes localized in immunogenic regions of the envelope. METHODS: PCR-amplified envelope regions of 5
Comparison of HTLV-I/II infection by serology & PCR.: Int Conf AIDS. 1991 Jun 16-21;7(1):29 (abstract no. M.A.29). Unique Identifier : AIDSLINE ICA7/1002991; Smith D; Mann T; Anderson E; Canavaggio M; Lee H; Abbott Laboratories, North Chicago, IL; OBJECTIVE: HTLV-I EIA screening assays and the confirmatory procedures of Western blot (WB) and radioimmunoprecipitation (RIPA) show reactivity with antibodies to HTLV-I and the closely related HTLV-II. Patterns of serologic reactivity were compared to HTLV-I or II PCR results. METHODS: 181 serologically confirmed sam
Loss of antibody reactivity against cytomegalovirus during asymptomatic and late phases of HIV-1 infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):29 (abstract no. M.A.30). Unique Identifier : AIDSLINE ICA7/1003091; Fehniger TE; Converse P; Strannegard O; Ehrnst E; Maasho K; Sonnerborg A; Britton S; Karolinska Institute, Dept. of Infectious Diseases; OBJECTIVE: To define the patterns of antibody reactivity against CMV in HIV-1 infected subjects. METHODS: The study group of CMV seropositive subjects included HIV+ clinically asymptomatic (asym: n=13), ARC/AIDS (n=7), and HIV- homosexual and non-homosexual adul
Interactions of HIV and HPV in the development of penile cancers.: Int Conf AIDS. 1991 Jun 16-21;7(1):29 (abstract no. M.A.31). Unique Identifier : AIDSLINE ICA7/1003191; Tornesello ML; Beth-Giraldo E; Galloway DA; McDougall JK; Buonaguro FM; Giraldo G; Natl. Cancer Institute Fond. Pascale, Naples, Italy; OBJECTIVE: Analysis of the interactions and the role of HIVs and HPVs in the etiopathogenesis of penile cancers from patients of African regions with high prevalence of HIV infections and genital neoplasms. METHODS: To test the prevalence of HIVs and HPVs, DNAs extracted from Penile Cancers (PC) biopsies were tested b
HHV-6 as a potential cofactor in AIDS: transcriptional activation of CD4 and induction of susceptibility to HIV-1 infection in CD8+ T lymphocytes.: Int Conf AIDS. 1991 Jun 16-21;7(1):29 (abstract no. M.A.32). Unique Identifier : AIDSLINE ICA7/1003291; Lusso P; Malnati M; DeMaria A; Lori F; Garzino-Demo A; Arya S; Gallo RC; LTCB, NCI, Bethesda, MD; OBJECTIVE: To investigate the possible role of HHV-6 as a cofactor in AIDS and the interactions between HHV-6 and HIV-1. METHODS: Limiting dilution cell cloning, magnetic bead separation, fluorocytometry, Northern blot, PCR, p24 Ag capture, PFA inhibition, DEAE-Dextran transfection. RESULTS: Productive HHV-6 infection
Identification of a 90K tumor associated antigen in HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):30 (abstract no. M.B.33). Unique Identifier : AIDSLINE ICA7/1003391; Iacobelli S; Natoli C; Tinari N; Ghinelli F; Sighinolfi L; Bicocchi R; Ortona L; Antinori A; Tamburrini E; Cattedra di Oncologia Medica, Universita di Chieti; A 90.000 molecular weight protein (90K), detected by a monoclonal antibody has been identified in the sera of patients with various malignancies. We recently observed 3 cases of Kaposi s sarcomas who had the highest 90K levels yet recorded in our laboratory. This prompted us to investigate this antigen in HIV infectio
Acid hydrolysis increases the sensitivity of p24 antigen detection for the evaluation of antiviral therapy in asymptomatic HIV-1 infected individuals.: Int Conf AIDS. 1991 Jun 16-21;7(1):30 (abstract no. M.B.34). Unique Identifier : AIDSLINE ICA7/1003491; Bollinger RC; Kline R; Francis H; Moss M; Bartlett JG; Quinn T; Johns Hopkins University, Baltimore, MD; OBJECTIVE: The sensitivity of p24 antigen detection in asymptomatic HIV infected individuals is decreased by the presence of HIV-specific antibody. We examined the effect of acid pretreatment on the sensitivity of antigen detection in asymptomatic HIV infected patients in order to evaluate the effect of
Serial CD4 counts in relation to the decision to treat asymptomatic HIV-1 infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):30 (abstract no. M.B.35). Unique Identifier : AIDSLINE ICA7/1003591; Aledort LM; Mount Sinai Medical Center, New York City, NY; A rapid, steady decline in CD4 usually precedes the onset of AIDS. This suggests that a large change between visits may indicate imminent progression, and identify the asymptomatic persons most likely to benefit from anti-retroviral therapy. We tested the hypothesis by determining the individual and combined predictiv
Human herpes virus 6 and markers of disease progression in HIV-positive males.: Int Conf AIDS. 1991 Jun 16-21;7(1):30 (abstract no. M.B.36). Unique Identifier : AIDSLINE ICA7/1003691; Fairfax M; Schacker T; Cone R; Collier A; Corey L; Departments of Laboratory Medicine and Medicine, University of; Washington School of Medicine, Seattle, Washington, USA; OBJECTIVE: To investigate the natural history of human herpes virus 6 (HHV-6) in HIV-infected males by comparing the amount of HHV-6 DNA in blood and saliva with stage of disease, T cell subsets, anti-viral therapy, and serology. METHODS: Blood and saliva were collected from 32 HIV-positive males. Semi-quantitative po
Nearly all deaths in patients (pts) on anti-HIV therapy occurred in individuals with less than 50 CD4 cells/mm(3) in the adult NCI experience: implications for the development of CD4 as a mortality risk indicator.: Int Conf AIDS. 1991 Jun 16-21;7(1):31 (abstract no. M.B.37). Unique Identifier : AIDSLINE ICA7/1003791; Yarchoan R; Venzon D; Lietzau J; Pluda JM; Wyvill KM; Steinberg S; Broder S; National Cancer Institute (NCI), Bethesda, MD, USA; OBJECTIVE: Depletion of CD4 cells is central in the pathogenesis of AIDS. However, there is relatively little information available on the relationship between CD4 cells and the immediate hazard of dying. METHODS: Records of 55 consecutive pts with AIDS or severe ARC on AZT-based regimens treated between July 1985 and
A sensitive method for monitoring efficacy of anti-retroviral therapy in HIV-infected individuals: a highly sensitive p24 antigen assay.: Int Conf AIDS. 1991 Jun 16-21;7(1):31 (abstract no. M.B.38). Unique Identifier : AIDSLINE ICA7/1003891; Winger EE; Reddy MM; Hargrove D; McHugh T; McKinley GF; Grieco MH; Immunodiagnostic Laboratories, Inc., San Francisco, California,; USA; OBJECTIVE: Current methods for monitoring of HIV viral burden include quantitative PCR, quantitative plasma viral culture and HIV p24 antigen assay. The first two techniques are currently impractical while the latter is relatively insensitive detecting antigen in only a fraction of infected individuals. METHODS: A hig
Estimate of HIV-1 infection among U.S. hospital patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):32 (abstract no. M.C.39). Unique Identifier : AIDSLINE ICA7/1003991; Stafford RS; Janssen RS; St Louis ME; Petersen LR; Dondero TJ; the Sentinel Hospital Surveillance Group, Centers for Disease; Control, Atlanta, GA, USA; OBJECTIVE: To estimate the annual number of HIV-1 infected patients admitted to U.S. hospitals. METHODS: As a modified part of CDC s Sentinel Hospital Survey, from 7/89 to 6/90 a systematic sample of blood specimens left over after clinical testing were anonymously tested for antibodies to HIV-1. Sera from 52,500 pati
Intervillage variations in HIV seroprevalence in a rural Ugandan community.: Int Conf AIDS. 1991 Jun 16-21;7(1):32 (abstract no. M.C.40). Unique Identifier : AIDSLINE ICA7/1004091; Kengeya-Kayondo JF; Kamali A; Nunn AJ; Mulder DW; MRC (UK) Programme on AIDS in Uganda; AIMS: The present study examines between-village variation in HIV seroprevalence and explanatory variables for the observed variation. The study is part of the MRC Programe on AIDS which aims, among other objectives, at elucidating the population dynamics of HIV infection. STUDY METHODS: The population cohort covers a
Direct measurement of incidence of HIV-1 and HIV-2 in female prostitutes in Senegal.: Int Conf AIDS. 1991 Jun 16-21;7(1):32 (abstract no. M.C.41). Unique Identifier : AIDSLINE ICA7/1004191; Kanki P; M'Boup S; Marlink R; NDoye I; Gueye A; Siby T; Thior I; Dia M; Travers K; Essex M; Harvard School of Public Health, Boston, MA, USA; OBJECTIVE: We have followed registered female prostitutes in urban centers in Senegal to better understand the epidemiology of HIV-2 infection. The direct measurement of HIV-2 and HIV-1 incidence allows for a better understanding of HIV population dynamics in this endemic area. ME
Trends in HIV-1 and HIV-2 infections in Abidjan, Cote D'Ivoire, 1987-1990.: Int Conf AIDS. 1991 Jun 16-21;7(1):32 (abstract no. M.C.42). Unique Identifier : AIDSLINE ICA7/1004291; Doorly R; Kadio A; Brattegaard K; Gnaore E; Coulibaly IM; DeCock KM; Projet RETRO-CI, Abidjan; OBJECTIVE: To examine trends in HIV-1 and HIV-2 infections in Abidjan between 1987 and 1990. METHODS: Serum specimens collected between 1987-1990 from 18,045 persons in Abidjan were tested for HIV-1 and HIV-2 antibodies (ELISA; Western blot and synthetic peptide suppemental tests). Groups tested included women of repr
The magnitude of HIV/AIDS epidemic in eastern part of Europe.: Int Conf AIDS. 1991 Jun 16-21;7(1):33 (abstract no. M.C.43). Unique Identifier : AIDSLINE ICA7/1004391; Gromyko A; World Health Organization, Regional Office for Europe; By the end of 1990, a total of 41 564 cases of AIDS were reported to the World Health Organization from 31 European countries. Of these only 1466 AIDS cases were reported from the eastern part of Europe, which includes Albania ,
United States Army: 1985-90.: Int Conf AIDS. 1991 Jun 16-21;7(1):33 (abstract no. M.C.44). Unique Identifier : AIDSLINE ICA7/1004491; McNeil J; Brundage J; Gardner L; Wann F; Renzullo P; Burke D; Walter Reed Army Institute of Research, Washington, DC, USA; OBJECTIVE: To describe secular trends and demographic correlates of an evolving HIV infection epidemic among soldiers in the United States Army. METHODS: Because soldiers in the US Army are recurrently tested for the presence of HIV antibody, HIV seroconversion rates can be directly me
Risk of developing AIDS after HIV seroconversion in injecting drug users: analysis of early markers of disease evolution.: Int Conf AIDS. 1991 Jun 16-21;7(1):33 (abstract no. M.C.45). Unique Identifier : AIDSLINE ICA7/1004591; Rezza G; Pezzotti P; Lazzarin A; Angarano G; Sinicco A; Aiuti F; Pristera R; Salassa B; Ortona L; Castelli F; et al; IMCSA, AIDS Unit, Istituto Superiore di Sanita-Roma (Italy); OBJECTIVE: To examine the risk of developing AIDS, the incubation period to full-blown disease, and risk factors for disease progression among a cohort of Italian seropositive injecting drug users (IDUs). METHODS: A longitudinal study was conducted among IDUs, enrolled in 15 clinical centres in
Sex trade activity among female injection drug users in San Francisco.: Int Conf AIDS. 1991 Jun 16-21;7(1):33 (abstract no. M.C.46). Unique Identifier : AIDSLINE ICA7/1004691; Lorvick J; Watters J; Shade S; Cheng YT; University of California, San Francisco; OBJECTIVE: To measure the frequency of sex-for-money activity among a sample of female injection drug users (IDUs) and examine its relationship to condom use, history of sexually transmitted diseases and rate of HIV infection. METHODS: Using a targeted sampling method, two cross-sections of female IDUs were recruited
HIV wasting syndrome in the United States.: Int Conf AIDS. 1991 Jun 16-21;7(1):34 (abstract no. M.C.47). Unique Identifier : AIDSLINE ICA7/1004791; Nahlen B; Nwanyanwu O; Chu S; Berkelman R; Centers for Disease Control (CDC), Atlanta, Georgia, USA; OBJECTIVE: To describe the characteristics of AIDS patients reported with HIV wasting syndrome (WS) in the United States . METHODS: Adult/adolescent AIDS patients (greater than 13 years) reported with WS to CDC from 9/87 through 12/90 were compared to those without WS (non-WS). RESULTS:
Prevalence of HIV infection among injecting drug users in London.: Int Conf AIDS. 1991 Jun 16-21;7(1):34 (abstract no. M.C.48). Unique Identifier : AIDSLINE ICA7/1004891; Crosier AN; Stimson GV; Ettorre EM; Stephens SF; Centre for Research on Drugs and Health Behaviour, Charing Cross,; England; OBJECTIVE: To determine the prevalence of HIV infection and describe associated risk behaviours among injecting drug users (IDUs) in London, England, in collaboration with the WHO Study on Drug Injecting and Risk of HIV Infection. METHODS: 534 IDUs were recruited, 117 (22%) from 13 treatment sites and 417 (88%) from o
Bleach disinfection of needles by intravenous drug users: association with HIV seroconversion.: Int Conf AIDS. 1991 Jun 16-21;7(1):34 (abstract no. M.C.49). Unique Identifier : AIDSLINE ICA7/1004991; Vlahov D; Celentano DD; Munoz A; Cohn S; Anthony JC; Nelson KE; ALIVE Study, Johns Hopkins School of Hygiene and Public Health,; Baltimore, MD, USA; OBJECTIVE: To examine association between use of bleach disinfection of needles and HIV seroconversion (SC) among intravenous drug users (IVDUs). METHODS: IVDUs initially screened in 1988-89 were scheduled to return for serological rescreens and risk behavior interviews at 6 mos. intervals. The sample for analysis was
The risk that HIV-seropositive drug users (DUs) transmit their HIV-infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):34 (abstract no. M.C.50). Unique Identifier : AIDSLINE ICA7/1005091; Hartgers C; Krijnen P; VandenHoek JA; Coutinho RA; VanderPligt J; Municipal Health Service, Amsterdam, The Netherlands; OBJECTIVE: To determine rates and potential predictors of HIV-transmission risk behavior in HIV-seropositive (HIV+) DUs (n=122) enrolled in a longitudinal Amsterdam AIDS-study. METHODS: Data on current (i.e. in the previous 4-6 months) sexual and injecting behavior, perception of HIV-transmission risk, self- and respo
African AIDS policies and legal traditions.: Int Conf AIDS. 1991 Jun 16-21;7(1):35 (abstract no. M.D.51). Unique Identifier : AIDSLINE ICA7/1005191; Shandera WX; Dept of Medicine, Baylor College of Medicine, Houston, TX USA; OBJECTIVE: To assess current African AIDS policies and determine if policies correlate with legal traditions of responding countries. METHODS: African ministries of health were contacted twice in 1990 regarding HIV/AIDs policies dealing with notification of health authorities and sexual contacts, anti-discrimination,
The Food and Drug Administration's community based research efforts.: Int Conf AIDS. 1991 Jun 16-21;7(1):35 (abstract no. M.D.52). Unique Identifier : AIDSLINE ICA7/1005291; Couig MP; U.S. Food and Drug Administration, Rockville, MD, USA; OBJECTIVE: To expedite access to new therapies for HIV infection and AIDS-related illnesses by expanding access to investigational therapies and by increasing the size of the available data base. Community-based health professionals receive education, training and technical assistance. Information is provided on the
Balancing fear and responsibility: professional ethics in AIDS care.: Int Conf AIDS. 1991 Jun 16-21;7(1):35 (abstract no. M.D.53). Unique Identifier : AIDSLINE ICA7/1005391; Koenig BA; Cooke M; Beery N; Folkman S; University of California, San Francisco, CA, USA; OBJECTIVE: To assess the relationship between fear of AIDS and perceived professional responsibility among U.S. internal medicine residents, and to describe the process of accommodation to AIDS fear while caring for HIV-infected patients. METHODS: A self-administered survey was completed by 40% of medical trainees at
Increasing dentists' willingness to treat HIV+ patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):35 (abstract no. M.D.54). Unique Identifier : AIDSLINE ICA7/1005491; Sadowsky D; Kunzel C; Albert Einstein College of Medicine, Bronx, New York, USA; OBJECTIVE: What predicts dentists willingness to treat HIV+ patients (PHIV+)? A model has been developed to answer the question and is described below. METHODS: A disproportionately stratified (high AIDS prevalence urban, other urban, and rural areas) random sample of American general practitioner dentists was drawn
AIDS, euthanasia and grief.: Int Conf AIDS. 1991 Jun 16-21;7(1):36 (abstract no. M.D.55). Unique Identifier : AIDSLINE ICA7/1005591; VandenBoom FM; Mead C; Gremmen T; Roozenburg H; Netherlands Institute of Mental Health, Utrecht, The Netherlands; OBJECTIVE: Gain insight in 1) the occurrence of euthanasia among Aids patients within the context of Dutch euthanasia policy; 2) the grieving process and its complications. METHODS: In-depth structured interviews were held with 59 relatives of deceased Aids patients. RESULTS: Euthanasia was discussed by 60% of all pat
Factors effecting the decision and intention to seek an HIV Ab test.: Int Conf AIDS. 1991 Jun 16-21;7(1):36 (abstract no. M.D.56). Unique Identifier : AIDSLINE ICA7/1005691; Myers T; Orr K; Locker D; Jackson E; University of Toronto, Toronto, Ont., Canada; OBJECTIVE: To identify among gay and bisexual men the socio-demographic and behavioural variables related to a previous decision or future intention to be tested and to reasons for not being tested. METHODS: A convenience sample of 1295 men who have sex with men was systematically recruited in Toronto s gay identified
Teleconferencing: how 8000 physicians can gain access to the latest medical education on HIV care and treatment.: Int Conf AIDS. 1991 Jun 16-21;7(1):36 (abstract no. M.D.57). Unique Identifier : AIDSLINE ICA7/1005791; Allerton M; Wolfe E; Wibbelsman C; McNalty K; Gordon N; Fuller J; Casal T; Kaiser Permanente Medical Care Program (KPMCP), Northern; California Region, Oakland, CA. USA; OBJECTIVE: To increase the number of physicians in a large group practice (n=3000) willing and able to provide primary medical care for HIV infected patients and their families in Northern California KPMCP, a Health Maintenance Organization with 2.4 million members (estimated HIV sero-prevelence of 0.5%) and to make H
Observed and self-reported compliance with universal precautions among emergency department personnel at two suburban community hospitals.: Int Conf AIDS. 1991 Jun 16-21;7(1):36 (abstract no. M.D.58). Unique Identifier : AIDSLINE ICA7/1005891; Henry K; Collier P; O'Boyle-Williams C; Campbell S; University of Minnesota Medical School, Minneapolis, MN USA; OBJECTIVE: To examine the compliance of Emergency Department (ED) personnel with the Universal Precautions (UP) recommendations derived from CDC guidelines. METHODS: From 6/90-8/90, ED personnel were observed by 7 RNs at 2 medical centers in suburban Minneapolis. In 8/90, a survey was sent to the same personnel to det
Risk of endemic HIV and hepatitis B virus (HBV) transmission to patients during invasive procedures.: Int Conf AIDS. 1991 Jun 16-21;7(1):37 (abstract no. M.D.59). Unique Identifier : AIDSLINE ICA7/1005991; Bell DM; Martone WJ; Culver DH; Margolis HS; Shapiro CN; Tokars JI; Marcus R; Furman LJ; Jaffe HW; Curran JW; et al; Centers for Disease Control, Atlanta, GA, USA; OBJECTIVE: To estimate 1) the probability (Pr) of endemic HIV and HBV transmission to patients from an infected surgeon or dentist due to percutaneous injury during an invasive procedure; 2) the number of patients infected by this route in the USA between 1980-1990. METHODS: Risk was estimated using data from prospect
Transmission of HIV infection from surgeon to patient: estimating the risk.: Int Conf AIDS. 1991 Jun 16-21;7(1):37 (abstract no. M.D.60). Unique Identifier : AIDSLINE ICA7/1006091; Lowenfels AB; Wormser GP; Departments of Surgery and Division of Infectious Disease,; Department of Medicine, NY Medical College, Valhalla NY. 10595; OBJECTIVE: What is the likelihood of a patient becoming HIV+ as a result of a puncture wound to the surgeon during a typical one hour operative procedure? METHODS: We have used published reports to calculate the overall probability of such an event which depends upon: 1) the probability of a puncture injury during sur
Efficacy of HIV transmission after at-risk exposures in health care settings: the Italian multicentric study.: Int Conf AIDS. 1991 Jun 16-21;7(1):37 (abstract no. M.D.61). Unique Identifier : AIDSLINE ICA7/1006191; Ippolito G; Puro V; Angarno G; Arici C; Bertucci R; Bonazzi L; Cadrobbi P; Cestrone A; Chiodera A; Chiriann A; et al; Coordinating Center; AIDS Unit at L. Spallanzani Hospital, Rome,; Italy; OBJECTIVE: To evaluate efficacy of HIV transmission after exposures to at/risk body fluids in health care workers (HCW), and to study incidence and type of exposures. METHODS: Data were collected prospectively by 30 hospital occupational health services. All exposed HCW were tested for HIV/Ab at the moment of the acci
Low rate of HIV-1 infection among health-care workers who donate blood.: Int Conf AIDS. 1991 Jun 16-21;7(1):37 (abstract no. M.D.62). Unique Identifier : AIDSLINE ICA7/1006291; Chamberland M; Petersen L; Munn V; White C; Busch M; Grindon A; Kamel H; Ness P; Zeger G; Centers for Disease Control, Atlanta, GA; OBJECTIVE: To estimate the rate of occupationally-acquired HIV-1 infection among health-care workers (HCWs) who are blood donors (BDs). METHODS: In an ongoing study, HIV-1 seropositive BDs at 20 U.S. blood centers (BCs) are interviewed using a standard protocol to assess HIV risk factors. In these 20 BCs, 1.7% of bloo
Influence of carbohydrate moieties on the immunogenicity of HIV-1 recombinant GP160 (rgp160).: Int Conf AIDS. 1991 Jun 16-21;7(1):38 (abstract no. M.A.63). Unique Identifier : AIDSLINE ICA7/1006391; Benjouad A; Gluckman JC; Rochat H; Montagnier L; Bahraoui E; CERVI Pitie-Salpetriere Medical School, Paris, France; OBJECTIVE: To investigate the specificity and neutralizing capacity of antibodies raised against native, or partially or totally deglycosylated rgp160 of HIV-1 Bru. METHODS: Rabbits were immunized with rgp160 or deglycosylated derivatives obtained by either neuraminidase, or mannosidase or Endo F-N glycanase. Specific
Development of an AIDS vaccine candidate: recombinant BCG vaccine vehicles induce humoral and cell-mediated immune responses to HIV antigens.: Int Conf AIDS. 1991 Jun 16-21;7(1):38 (abstract no. M.A.64). Unique Identifier : AIDSLINE ICA7/1006491; Aldovini A; Young R; Whitehead Institute for Biomedical Research, Department of; Biology, Massachusetts Institute of Technology, Cambridge, MA USA; We have used the potent immunological properties of the human tuberculosis vaccine Mycobacterium bovis BCG to develop a live recombinant vaccine vehicle that can produce selected pathogen proteins and stimulate the mammalian immune system to respond to them. BCG has a number of features that make it a particularly att
Promiscuity of cytotoxic T-cell epitopes from the HIV-1 envelope.: Int Conf AIDS. 1991 Jun 16-21;7(1):38 (abstract no. M.A.65). Unique Identifier : AIDSLINE ICA7/1006591; Berzofsky JA; Shirai M; Metabolism Branch, NCI, NIH, Bethesda, MD, USA; OBJECTIVE: We observed that an immunodominant cytotoxic T lymphocyte (CTL) epitope of HIV-1 gp160, P18 (residues 315-329), is presented by class II major histocompatibility complex (MHC) molecules to helper T cells as well as by class I MHC molecules to CTL, and that several peptides of gp160 identified as helper epit
Induction of anti HIV antibodies by immunization with monoclonal anti-idiotypes.: Int Conf AIDS. 1991 Jun 16-21;7(1):38 (abstract no. M.A.66). Unique Identifier : AIDSLINE ICA7/1006691; Zaghouani H; Bona C; Department of Microbiology, Mount Sinai School of Medicine, New; York USA; Anti-idiotypes which possess the internal image of an antigen can induce protective humoral immunity toward bacterial, viral and parasitic infections. Herein, we demonstrate antigen mimicry by monoclonal anti-idiotypes to a distinct epitope of the HIV envelope protein which is defined by a synthetic peptide. This pept
Progress report IV on AIDS vaccine in human: phase I clinical trial in HIV infected patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):39 (abstract no. M.A.67). Unique Identifier : AIDSLINE ICA7/1006791; Zagury D; Picard O; Halbreich A; Bernard J; Carelli C; Bizinni B; Salaun JJ; Lurhuma Z; Mbayo K; Burny A; et al; Universite Pierre et Marie Curie, Paris, France; Vaccine trial phase I performed in AIDS patients, using autologous cells expressing HIV1 antigens was performed at the University Clinic of Kinshasa and at Hospital Saint Antoine. Clinical and biological benefit was observed(a,b), but no repair of specific cellular immunity was apparent as if 1) the benefit was due to
Construction of immunogenic synthetic HIV vaccine candidates.: Int Conf AIDS. 1991 Jun 16-21;7(1):39 (abstract no. M.A.68). Unique Identifier : AIDSLINE ICA7/1006891; Charles S; Matthews T; Chong P; Haynes J; Rovinski B; Cao SX; Bolognesi D; Klein M; Connaught Centre for Biotechnology Research, 1755 Steeles Avenue; West, Willowdale, Ont. M2R 3T4, Canada; OBJECTIVE: To construct synthetic HIV vaccine candidates using defined T- and B-cell epitopes of HIV structural proteins. METHODS: Chimeric oligopeptides containing either a gp120 or a gp41 B-cell epitope from the MN and LAV isolates, respectively, linked in tandem to either the N- or C-terminus of a defined p24 T-cel
Characterization of human antibody binding sites on the HIV-2 external envelope.: Int Conf AIDS. 1991 Jun 16-21;7(1):39 (abstract no. M.A.69). Unique Identifier : AIDSLINE ICA7/1006991; deWolf F; Meloen RH; Bakker M; Barin F; Goudsmit J; Human Retrovirus Laboratory, University of Amsterdam, The; Netherlands; OBJECTIVE: To map linear antibody binding sites on the HIV-2 external glycoprotein (EGP) gp125 and to study antibody reactivity to the most antigenic epitopes in sera of HIV-1- and HIV-2-infected individuals. METHODS: HIV-2 immunoblot positive sera from 8 patients were used for antibody-reactive peptide scanning (PEPS
Human monoclonal antibodies against the putative CD4 binding site and the V3 loop of HIV gp120 act in concert to neutralize virus.: Int Conf AIDS. 1991 Jun 16-21;7(1):39 (abstract no. M.A.70). Unique Identifier : AIDSLINE ICA7/1007091; Tilley SA; Honnen WJ; Racho ME; Hilgartner M; Pinter A; Public Health Research Institute, New York, NY; OBJECTIVE: To develop human monoclonal antibodies (HuMAbs) against HIV with potent neutralizing and/or other anti-viral activities. METHODS: HuMAbs were derived by EBV transformation of PBMC from asymtomatic, seropositive hemophiliacs. The specificity of HuMAbs was determined by ELISAs, Western blot and RIPA analyses,
HIV-1 load in the blood is inversely related to the level of reverse transcriptase inhibiting (RTI) antibody.: Int Conf AIDS. 1991 Jun 16-21;7(1):40 (abstract no. M.A.71). Unique Identifier : AIDSLINE ICA7/1007191; Imagawa DT; Kobata H; Lee M; Ueda M; Javier B; Russo F; Aldrovandi G; Detels R; Harbor-UCLA Medical Center, Torrance, CA; OBJECTIVE: Infectious HIV-1 load in the peripheral blood mononuclear cells (PBMC) and titer of RTI in the corresponding plasma were evaluated in 54 asymptomatic seropositive homosexual men. METHODS: Limiting dilutions of PBMC were cocultured with PHA- stimulated donor cells in a microculture procedure to quantitate th
Mouse monoclonal antibody (G3-4) defines a novel conformation-dependent epitope on gp120 important for neutralization of divergent HIV-1 isolates.: Int Conf AIDS. 1991 Jun 16-21;7(1):40 (abstract no. M.A.72). Unique Identifier : AIDSLINE ICA7/1007291; Ho DD; Fund MS; Li XL; Sun C; Chang NT; Chang TW; Sun NC; The Aaron Diamond AIDS Research Center and New York University; School of Medicine, New York, New York, USA; OBJECTIVE: To define domains in gp120 important for antibody neutralization of divergent HIV-1 isolates. METHODS: G3-4 monoclonal antibody was generated by immunizing mice with purified gp120 of HIV-1 (IIIB), and it has been extensively characterized in radioimmunoprecipitation, neutralization, and gp120-CD4-binding i
V3 epitopes can mediate antibody neutralization and enhancement of HIV-1 infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):40 (abstract no. M.A.73). Unique Identifier : AIDSLINE ICA7/1007391; Kliks SC; Wu WW; Levy JA; University of California, School of Medicine, San Francisco, CA,; USA; OBJECTIVE: To investigate the mechanism of strain-specific antibody neutralization and enhancement of infection by HIV-1 strains. The studies were undertaken because sera from humans and primates immunized with HIV-1 envelope glycoproteins have been found to have dual properties. METHODS: Human anti V3 monoclonal anti
Inhibition of HIV-1 infection by immunological reagents.: Int Conf AIDS. 1991 Jun 16-21;7(1):40 (abstract no. M.A.74). Unique Identifier : AIDSLINE ICA7/1007491; Ugen KE; Goedert J; Kieber-Emmons T; Merva M; Boyer J; Williams WV; Weiner DB; The Wistar Institute, Department of Medicine, University of; Pennsylvania, Philadelphia, PA; OBJECTIVE: Understanding the contribution of the humoral immune response to the functions of the HIV envelope in HIV mediated viral entry processes. Anti-syncytial and group specific neutralization activity are observed most frequently in the asymptomatic patient population. High immune responses to the V3 loop region
Persistent inapparent HIV-1 infection of human neuroblastoma cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):41 (abstract no. M.A.75). Unique Identifier : AIDSLINE ICA7/1007591; Vesanen M; Linna T; Vaheri A; Department of Virology University of Helsinki, Helsinki, Finland; We have studied human immunodeficiency virus type 1 (HIV-1) infection in three different human neuroblastoma cell lines; SK-N-MC, IMR-32 and SH-SY5Y. In all of these cell lines the infection became productive. However, the virus expression was different as determined by the p24 antigen capture assays from culture supe
Neutralization of HIV-1 infection by anti-V3 loop antibody is dependent upon the host cell of the viral inoculum.: Int Conf AIDS. 1991 Jun 16-21;7(1):41 (abstract no. M.A.76). Unique Identifier : AIDSLINE ICA7/1007691; Ohashi K; Popovic M; Liu Y; Hunter E; Javaherian K; Eddy G; Gartner S; Fujisaki Institute, Okayama, Japan; OBJECTIVE: We sought to determine whether antibodies capable of neutralizing virus infection of T cells can also influence infection of mononuclear phagocytes. METHODS: HIV-1LW-PBM, an isolate from a lab worker infected with HTLV-IIIB was used. To generate viral stocks, early passage virus was transmitted to monocyte/
Synthetic peptides derived from the principal neutralizing domain (PND) of human immunodeficiency virus type 1 (HIV-1) enhance HIV-1 infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):41 (abstract no. M.A.77). Unique Identifier : AIDSLINE ICA7/1007791; DeRossi A; Pasti M; Mammano F; Panozzo M; Dettin M; DiBello C; Chieco-Bianchi L; Institute of Oncology, Institute of Industrial Chemistry,; University of Padova, Italy; OBJECTIVE: To investigate biological properties of PND from different HIV-1 strains. METHODS: 24-mer peptides derived from PND (301-324 aminoacid sequence) of different HIV-1 strains were synthesized on solid support using a peptide synthetizer (Applied Biosystems 431A). Target cell (MOLT-3 and H938) infection with MN
CD4 independent fusion and infection by HIV-2 strains: enhancement by soluble CD4.: Int Conf AIDS. 1991 Jun 16-21;7(1):41 (abstract no. M.A.78). Unique Identifier : AIDSLINE ICA7/1007891; Clapham P; McKnight A; Weiss R; Chester Beatty Laboratories, Institute of Cancer Research, Fulham; Road, London SW3 6JB, UK; OBJECTIVE: We have characterized HIV-2 fusion and infection of CD4-negative human cell lines. METHODS: HIV-2 strains used were LAV-2ROD, SBL6669, CBL-20, CBL-21, CBL-22 and CBL-23. Human cells included RD/TE671 rhabdomyosarcoma, U87 glioma, HeLa carcinoma, Raji and Daudi B cell lines. HIV-2 fusion was tested by overni
Maternal-fetal HIV-transmission: placental cells CD4 expression and permissivity to infection with HIV.: Int Conf AIDS. 1991 Jun 16-21;7(1):42 (abstract no. M.A.79). Unique Identifier : AIDSLINE ICA7/1007991; David FJ; Tran HC; Autran B; Raphael M; Menu E; Hsi BL; Barre-Sinoussi F; Chaouat G; INSERM U 262, Clin. Univ. Baudelocque, Paris; OBJECTIVE: To investigate the molecular mechanisms allowing the transplacental passage of HIV, we studied: A- The placental Fc-Receptors (Fc-R), possibly involved in the placental passage of IgG-complexed viral particles. B- The expression of CD4 and the permissivity of placental cells to infection with HIV. METHODS:
Regulation of silent and productive HIV-1 infection in primary monocytes by two distinct genetic determinants.: Int Conf AIDS. 1991 Jun 16-21;7(1):42 (abstract no. M.A.80). Unique Identifier : AIDSLINE ICA7/1008091; Westervelt P; Trowbridge DB; Gendelman HE; Ratner L; Departments of Medicine & Molecular Microbiology, Washington; University School of Medicine, St. Louis, MO; OBJECTIVE: Determination of the molecular basis for HIV-1 tropism for primary human monocytes. METHODS: Recombinant proviral clones were constructed by reciprocal DNA fragment exchange between a monocyte-tropic clone (ADA) and two full-length molecular clones (HXB2 and NL4-3) incapable of infection or replication in p
Clinical and autoptical diagnosis in AIDS: autopsy warns the clinical approach.: Int Conf AIDS. 1991 Jun 16-21;7(1):43 (abstract no. M.B.81). Unique Identifier : AIDSLINE ICA7/1008191; d'Arminio MA; Vago L; Lazzarin A; Formenti T; Rizzardi GP; Guzzetti S; Musicco M; Costanzi G; Moroni M; Clin Inf Dis, Univ Milan, Milan, Italy; OBJECTIVE: To relate clinical and autoptical observations in AIDS patients, stratified according to the indicator diseases and the length of survival. Patients: 533 patients with AIDS since 1984 to Dec 31th, 1990 were included. 341 (64%) were PDA or ex-PDA, 150 (28.1%) homosexual men, 33 (6.2%) heterosexual and 9 (1.7
Bacteremia complicating HIV-infection: frequency, spectrum and outcome.: Int Conf AIDS. 1991 Jun 16-21;7(1):43 (abstract no. M.B.82). Unique Identifier : AIDSLINE ICA7/1008291; Phillips P; Le A; Duncan J; Oh H; Schechter MT; Montaner JS; AIDS Research Programme, St Paul's Hospital, University of; British Columbia, Vancouver, B.C., Canada; OBJECTIVE: To determine the frequency, spectrum, and outcome of bacteremia in HIV-infected individuals admitted to St. Paul s hospital in the 5 year period ending December, 1990. METHODS: Retrospective review of all hospital admissions of HIV-infected individuals. Bacteremia was defined as 2 or more separate blood cul
Toxoplasmic ocular infection in patients with AIDS.: Int Conf AIDS. 1991 Jun 16-21;7(1):43 (abstract no. M.B.83). Unique Identifier : AIDSLINE ICA7/1008391; Accorinti M; Pivetti-Pezzi P; Tamburi S; Ciapparoni V; D'Offizi GP; Tilia G; Catania S; Institute of Ophthalmology, University La Sapienza, Rome, Italy; OBJECTIVE: To define the characteristic clinical features, the therapeutical regimens and a possible neurological involvement in ocular toxoplasmosis. METHODS: 152 AIDS patients were submitted to an ophthalmological examination. All the subjects with ocular toxoplasmosis underwent also a neurological evaluation includ
Mortality in HIV-1 positive tuberculosis (TB) cases, Nairobi, Kenya.: Int Conf AIDS. 1991 Jun 16-21;7(1):43 (abstract no. M.B.84). Unique Identifier : AIDSLINE ICA7/1008491; Nunn P; Odhiambo J; Brindle R; Carpenter L; Gathua S; Wasunna K; Were J; McAdam K; Kenya Medical Research Institute, Nairobi, Kenya; OBJECTIVE: To compare mortality rates and causes among HIV+ and HIV- TB cases (PTBC and NTBC respectively). METHODS: 107 HIV PTBC and 174 HIV NTBC were enrolled in a longitudinal follow up study. Kaplan-Meier survival curves were estimated for both groups. Risk factors for mortality by 6 months were determined by prop
ACTG 095: a randomized comparison of foscarnet (FOS) vs. Vidarabine (ARA-A) for treatment of acyclovir (ACV)-resistant mucocutaneous herpes simplex virus (HSV) infection in patients with AIDS.: Int Conf AIDS. 1991 Jun 16-21;7(1):44 (abstract no. M.B.85). Unique Identifier : AIDSLINE ICA7/1008591; Safrin S; Crumpacker C; Chatis P; Davis R; Hafner R; Rush J; Kessler H; Landry B; Mills J; University of California, San Francisco CA; OBJECTIVE: The therapy of ACV-resistant HSV has not been studied in a controlled fashion. We compared FOS to ARA-A in a randomized, multicenter trial. METHODS: Patients with HSV lesions unresponsive to therapy with IV ACV for a minimum of 10 d and which showed in vitro resistance to ACV were randomized to receive FOS
A randomized, controlled study of immediate vs deferred ganciclovir therapy in AIDS patients with cytomegalovirus peripheral retinitis.: Int Conf AIDS. 1991 Jun 16-21;7(1):44 (abstract no. M.B.86). Unique Identifier : AIDSLINE ICA7/1008691; Spector SA; Barker C; Buhles W; Feinberg J; Montague P; Weingeist T; DeArmond B; U.C. San Diego; OBJECTIVE: To evaluate the efficacy of intravenous ganciclovir (GCV) in treating peripheral CMV retinitis in AIDS patients. METHODS: A randomized, multicenter, controlled trial was conducted in 42 subjects (39 males and 3 females) with CMV peripheral retinitis.
A single, rapid peptide-based immunoassay for the simultaneous detection of antibodies to HIV-1 and HIV-2.: Int Conf AIDS. 1991 Jun 16-21;7(1):45 (abstract no. M.C.87). Unique Identifier : AIDSLINE ICA7/1008791; Montana JP; Gosting L; Cole CA; Monji N; Su P; Ferrera C; Coleman PF; Genetic Systems Corporation, Redmond, WA., USA; OBJECTIVE: To develop a rapid (10 min.), synthetic peptide-based immunoassay for the separate and simultaneous detection of antibodies to HIV-1 and HIV-2 for diagnostic purposes. METHODS: Synthetic peptides representing highly conserved, immunodominant regions of HIV-1 gp41 and HIV-2 gp 36 were adsorbed to latex beads
Alternative screening and supplemental testing strategies for HIV-1 and HIV-2 infections: Int Conf AIDS. 1991 Jun 16-21;7(1):45 (abstract no. M.C.88). Unique Identifier : AIDSLINE ICA7/1008891; Brattegaard K; Doorly R; Kouadio J; Maran M; George R; Gayle H; De Cock KM; Projet RETRO-CI, Abidjan, Cote d'Ivoire; OBJECTIVE: (1) To evaluate rapid tests for HIV-1 and HIV-2 antibodies; (2) to evaluate use of synthetic peptide-based tests (SPT) as supplemental tests instead of Western blot (WB). METHODS: 1000 consecutive pregnant women in Abidjan were screened for HIV-1 and HIV-2 by reference strategy (ELISA/WB/SPT). All specimens
The use of saliva and urine for HIV surveillance in children.: Int Conf AIDS. 1991 Jun 16-21;7(1):45 (abstract no. M.C.89). Unique Identifier : AIDSLINE ICA7/1008991; Burns SM; Robertson P; Yap PL; Mok JQ; Parry J; Regional Virus Laboratory; OBJECTIVE: Children born to HIV infected mothers require follow-up until their HIV status is determined, and this may take up to 18 months. A study was carried out on a group of known infected and noninfected children to examine the feasibility of using saliva and urine samples as an alternative to blood. Subjects: Fo
A WHO International Quality Assessment Scheme for HIV antibody testing; results from the second distribution of sera.: Int Conf AIDS. 1991 Jun 16-21;7(1):45 (abstract no. M.C.90). Unique Identifier : AIDSLINE ICA7/1009091; Fauquex A; Snell JJ; Supran EM; Tamashiro H; Global Programme on AIDS, World Health Organization, Geneva,; Switzerland; OBJECTIVE: To evaluate WHO International Quality Assessment Scheme for HIV-1 antibody testing which was established to monitor the quality of laboratory performance in HIV testing in member states. METHODS: Twenty serum specimens, 10 of which contained antibody to HIV-1, were sent to 103 laboratories in six WHO Region
Proposed WHO criteria for interpreting results from western blot assays for HIV-1, HIV-2, HTLV-I/HTLV-II: Int Conf AIDS. 1991 Jun 16-21;7(1):46 (abstract no. M.C.91). Unique Identifier : AIDSLINE ICA7/1009191; Tamashiro H; Karam M; Esparza J; Global Programme on AIDS, World Health Organization, Geneva,; Switzerland; OBJECTIVE: To assess the WHO revised Western blot (WB) criteria for HIV-1, HIV-2, and HTLV-I/HTLV-II using sera collected from HIV-1 or HIV-2 endemic countries. METHODS: 136 serum samples considered WB-positive for HIV-2 by national criteria were collected from 14 countries with endemic HIV-2 and retested blinded in 2
Reliable diagnosis of pediatric HIV infection within four months of life by combined use of polymerase chain reaction and virus culture.: Int Conf AIDS. 1991 Jun 16-21;7(1):46 (abstract no. M.C.92). Unique Identifier : AIDSLINE ICA7/1009291; Boni J; Kind C; Jendis J; Schupbach J; Swiss National Center for Retroviruses, Swiss Federal Office of; Health and University of Zurich, Zurich; OBJECTIVE: To assess the value of PCR and virus culture in the early diagnosis of pediatric HIV-infection. METHODS: Virus culture (VC) [Ho et al. NEJM 1989;321:1621-5], PCR screening with gag, and PCR confirmation with LTR and env primers (all PCR blind and in duplicate) were done with PBMC of so far 33 children born
Maternal HIV infection and low CD4/CD8 ratio as risk factors for prematurity and stillbirth.: Int Conf AIDS. 1991 Jun 16-21;7(1):46 (abstract no. M.C.93). Unique Identifier : AIDSLINE ICA7/1009391; Temmerman M; Hawala D; Ndinya-Achola JO; Plummer F; Piot P; University of Nairobi, Kenya; OBJECTIVE: To determine whether maternal HIV infection is a risk factor for poor obstetrical outcome (POO) defined as prematurity and stillbirth, and is so, to define risk factors for POO in HIV(+) women. METHODS: Pregnant women less than 28 weeks pregnant were screened for HIV-antibody and syphilis seroreactivity at
Mortality among 2419 HIV-positive U.S. military service applicants: an update.: Int Conf AIDS. 1991 Jun 16-21;7(1):46 (abstract no. M.C.94). Unique Identifier : AIDSLINE ICA7/1009491; Dannenberg A; McNeil J; Brundage J; Johns Hopkins School of Public Health, Baltimore, MD, USA; OBJECTIVE: Long term follow-up is ongoing to assess cause-specific mortality among civilian applicants excluded from military service due to HIV infection compared to applicants excluded due to other unrelated medical conditions. METHODS: The 1985-1988 files of the National Death Index (NDI) were searched for 2419 HIV
AIDS within five years of seroconversion.: Int Conf AIDS. 1991 Jun 16-21;7(1):47 (abstract no. M.C.95). Unique Identifier : AIDSLINE ICA7/1009591; Phair J; Detels R; Jacobson L; Rinaldo C; Saah A; Munoz A; Multicenter AIDS Cohort Study, Chicago, IL; OBJECTIVE: Describe homosexual men with incident HIV-1 infection who develop AIDS within five years and compare behavioral, clinical and immunologic characteristics of those progressing to AIDS (grp A) with those seroconverters remaining AIDS-free (grp B). METHODS: Between entry into the study (4/84 - 3/85) and 7/89,
Preliminary results of the natural history of HIV infection in Ugandan infants.: Int Conf AIDS. 1991 Jun 16-21;7(1):47 (abstract no. M.C.96). Unique Identifier : AIDSLINE ICA7/1009691; Guay L; Ball P; Ndugwa C; Kenya-Mugisha; Hom D; Kataaha P; Goldfarb J; Vjecha M; Mmiro F; Olness K; Case Western Reserve University, Cleveland, Oh USA; OBJECTIVE: To determine the natural history of infants born to HIV infected women. METHODS: Selected pregnant women presenting in Kampala, Uganda were enrolled in a prospective study of HIV infected women and their infants. The HIV serostatus of the maternal study group (70% HIV+,
Cervical disease among HIV-infected women by immune status.: Int Conf AIDS. 1991 Jun 16-21;7(1):47 (abstract no. M.C.97). Unique Identifier : AIDSLINE ICA7/1009791; LaGuardia K; McGuinness K; Hunter D; The New York Hospital-Cornell Medical Center, New York, NY, USA; OBJECTIVE: To examine the relationship between T-helper cell (CD4) status and the prevalence of cervical intraepithelial neoplasia (CIN) and human papilloma virus (HPV) in HIV-infected women. METHODS: In order to more closely examine the relationship between cervical disease and HIV infection, a sample of HIV positive
Cervical squamous intraepithelial lesions (CSIL) and HIV-1 infection in Malawian women.: Int Conf AIDS. 1991 Jun 16-21;7(1):47 (abstract no. M.C.98). Unique Identifier : AIDSLINE ICA7/1009891; Chiphangwi J; Dallabetta G; Miotti P; Liomba G; Wangel AM; Saah A; Johns Hopkins University, Baltimore, USA; OBJECTIVE: To assess the association between CSIL and HIV-1 infection in Malawian women with heterosexually acquired HIV-1 infection. METHODS: 159 post-partum women (95 seronegative (SN) and 54 seropositive (SP)) followed in a longitudinal infant transmission study of HIV-1 were given a questionnaire and had a physica
Bisexual men with AIDS in the United States: epidemiology and their role in the AIDS epidemic in women.: Int Conf AIDS. 1991 Jun 16-21;7(1):48 (abstract no. M.C.99). Unique Identifier : AIDSLINE ICA7/1009991; Chu SY; Peterman T; Doll L; Buehler J; Centers for Disease Control (CDC), Atlanta, Georgia, USA; OBJECTIVE: To compare bisexual men and homosexual men with AIDS and to assess the relative importance of bisexual men in the sexual transmission of HIV to women. METHODS: AIDS cases reported to the CDC through June 1990 were examined. RESULTS: Among 65,389 men with AIDS who reported having had sex with men, 16,793 (26
HIV-1 infection rates in three hepatitis B (HB) vaccine cohorts-Amsterdam(AM), New York(NY) and San Francisco(SF), 1978-90: Int Conf AIDS. 1991 Jun 16-21;7(1):48 (abstract no. M.C.100). Unique Identifier : AIDSLINE ICA7/1010091; Koblin B; Van Griensven GJ; Hessol NA; Byers RH; Stevens CE; Coutinho RA; The NY Blood Center, NY, USA; OBJECTIVE: To compare baseline characteristics and incidence of HIV infection from 1978-90 among homosexual men in placebo-controlled HB vaccine trials carried out in AM, NY and SF from 1978-82. METHODS: Study subjects were all men who participated in HB vaccine trials (N) and subsequently enrolled in AIDS follow-up s
Understanding the high rates of HIV risk-taking among young gay and bisexual men: the young men's survey.: Int Conf AIDS. 1991 Jun 16-21;7(1):48 (abstract no. M.C.101). Unique Identifier : AIDSLINE ICA7/1010191; Hays RB; Kegeles S; Coates T; Center for AIDS Prevention Studies, University of California, San; Francisco, California USA; OBJECTIVE: To examine factors associated with unsafe sex among young gay and bisexual men and generate ideas for effective prevention approaches with this population. METHODS: The Young Men s Survey is a longitudinal survey of 400 gay/bisexual men aged 18 to 27, recruited by peers (through social networks, bars, unive
Sexual relationships with women in homosexually identified men. The probability of HIV transmission to other populations.: Int Conf AIDS. 1991 Jun 16-21;7(1):48 (abstract no. M.C.102). Unique Identifier : AIDSLINE ICA7/1010291; Izazola JA; Gortmaker S; Basanez R; Sepulveda J; Mexico Ministry of Health; OBJECTIVE: To discuss the main characteristics of homosexually identified men who also have sex with women, in order to describe the risk of HIV transmission to other populations (e.g. women). METHODS: A survey in homosexual gathering places like gay bars and discotheques was conducted in 6 mexican cities. 715 men wer
Effect of HIV post-test counseling on sexually transmitted disease (STD) reinfection for patients attending a STD clinic.: Int Conf AIDS. 1991 Jun 16-21;7(1):49 (abstract no. M.C.103). Unique Identifier : AIDSLINE ICA7/1010391; Otten M; Zaidi A; Wroten J; Witte J; Peterman T; Division of STD/HIV Prevention, Centers for Disease Control; (CDC), Atlanta, GA, USA; Background: STD patients are often tested for HIV infection and counseled about the results. In 1989, over 300,000 STD patients were tested for HIV using public support. Yet, the effect of this intervention is uncertain. OBJECTIVE: Measure the effect of post-test counseling (PTC) for HIV on acquisition of a new STD. M
Ethnographic and quantitative research to design an intervention for truckers in Zimbabwe.: Int Conf AIDS. 1991 Jun 16-21;7(1):49 (abstract no. M.C.104). Unique Identifier : AIDSLINE ICA7/1010491; Wilson D; Lamson N; Nyathi B; Sibanda A; Sibanda T; U. of Zimbabwe, Zimbabwe; OBJECTIVE: To combine survey and ethnographic research methods to design an intervention for long-distance truckers in Zimbabwe . METHODS: Semi-structured interview of 74 truckers, key informant discussions, focus groups and participant observations by ethnographer who accompanied
Suicidality and psychological outlook in long term survivors of AIDS.: Int Conf AIDS. 1991 Jun 16-21;7(1):50 (abstract no. M.D.105). Unique Identifier : AIDSLINE ICA7/1010591; Remien RH; Rabkin J; Katoff L; Williams J; HIV Center for Clinical & Behavioral Studies-NY State Psychiatric; Institute & Columbia University, NY, NY, USA; OBJECTIVE: To assess past and current thoughts about death and suicide, history of suicide attempts, current mood disorders and feelings of hopelessness in a population of gay men, unique in having lived with AIDS for at least three years (N=53). METHODS: Subjects were clients at Gay Men s Health Crisis (GMHC), the la
High socio-economic status (HSES) is a risk factor for HIV-1 infection but not for sexually transmitted diseases (STD) in Malawian women.: Int Conf AIDS. 1991 Jun 16-21;7(1):50 (abstract no. M.D.106). Unique Identifier : AIDSLINE ICA7/1010691; Dallabetta G; Odaka N; Hoover D; Chiphangwi J; Liomba G; Miotti P; Saah A; Johns Hopkins University, Baltimore, USA; OBJECTIVE: To examine the relationship between STD and HIV-1 infection in urban pregnant women in Malawi . METHODS: 5376 pregnant women presenting for prenatal care to a large urban hospital in Malawi were interviewed about demographics, medical and sexual history, had a physical/pe
Sociodemographic predictors of AIDS related knowledge and practice in Uganda: Int Conf AIDS. 1991 Jun 16-21;7(1):50 (abstract no. M.D.107). Unique Identifier : AIDSLINE ICA7/1010791; McCombie S; Rwakagiri F; Bukombi S; Cohn P; AIDSCOM, University of Pennsylvania, Philadelphia, PA USA; OBJECTIVE: To collect baseline data to plan and evaluate an AIDS in the Workplace Training program that uses a peer educator model. METHODS: Anonymous interviews on knowledge, attitudes, and practices related to AIDS were conducted with 623 randomly selected individuals (457 men, 166 women) in five organizations. A mu
A study of the psycho-social determinants of risk-taking and preventive behavior related to HIV and AIDS.: Int Conf AIDS. 1991 Jun 16-21;7(1):50 (abstract no. M.D.108). Unique Identifier : AIDSLINE ICA7/1010891; Anonymous; A study of the psycho-social determinants of risk-taking and preventive behavior related to HIV and AIDS has been undertaken in Metro-Manila. The sample included: 1. the general population consisting of 711 randomly selected households with 1,600 respondents 15-59 years old, using a panel from the National Statistics
Psychosocial correlates of HIV risk taking behavior.: Int Conf AIDS. 1991 Jun 16-21;7(1):51 (abstract no. M.D.109). Unique Identifier : AIDSLINE ICA7/1010991; Leserman J; Perkins DO; Boucvalt J; Evans DL; Department of Psychiatry, University of North Carolina, Chapel; Hill, NC, USA; OBJECTIVE: The goals of this study were twofold. We examined the relationship between psychosocial factors and 1) risk taking sexual activity within HIV negative men; and 2) sexual activity that exposes others to risk within HIV positive men. METHODS: As part of the Coping in Health and Illness project (CHIP), we stud
Who participates in surveys of HIV-exposing behaviors?: Int Conf AIDS. 1991 Jun 16-21;7(1):51 (abstract no. M.D.110). Unique Identifier : AIDSLINE ICA7/1011091; Biggar RJ; Melbye M; NCI, Bethesda, MD, USA; Typically, about 50% of potential participants in surveys of HIV-exposing behaviors will respond to mailed, self-administered questionnaires. The behaviors of those not responding introduce uncertainty about how well the results can be generalized to the whole population. In a random, stratified survey of Danes 18-59
The merging of public and academic institutions: a model of care for the HIV-infected child and mother.: Int Conf AIDS. 1991 Jun 16-21;7(1):51 (abstract no. M.D.111). Unique Identifier : AIDSLINE ICA7/1011191; Gath EC; Weber K; Martin A; Anderson D; Yogev R; Cook County Hospital (CCH), Chicago, Illinois, USA; OBJECTIVE: The establishment of a pediatric AIDS Clinical Trial Subunit (ACTU) of CMH at CCH, the only public hospital in Chicago primarily serving indigent poor, minority, and substance using populations. This Subunit provides investigational therapeutics to HIV-infected pregnant women and their children, currently u
Monitoring hospital resource use and cost: a three-and-a-half year follow-up.: Int Conf AIDS. 1991 Jun 16-21;7(1):51 (abstract no. M.D.112). Unique Identifier : AIDSLINE ICA7/1011291; Dijkgraaf MG; Borleffs JC; Poos MJ; Jager JC; Schrijvers AJ; Utrecht University Hospital, The Netherlands; OBJECTIVE: To determine trends in hospital resource use and cost related to treatment of HIV infected patients in a university hospital between January 1987 and June 1990. METHODS: Detailed data concerning hospital treatment of 126 HIV infected patients have been recorded prospectively over a three-and-a-half year per
Patients with AIDS and other HIV infections: examining and estimating the total burden of hospital care.: Int Conf AIDS. 1991 Jun 16-21;7(1):52 (abstract no. M.D.113). Unique Identifier : AIDSLINE ICA7/1011391; Andrulis D; Weslowski V; Hintz E; The National Public Health and Hospital Institute, Washington,; D.C., USA; OBJECTIVE: To assess the effect of the total HIV-infected patient caseload (i.e., AIDS and other HIV-related conditions) on hospitals; to document similarities and differences in utilization, demographics, financing and other factors between AIDS patients and individuals with other diagnoses related to HIV infection f
Predictors of AIDS rehospitalization in San Francisco (SF).: Int Conf AIDS. 1991 Jun 16-21;7(1):52 (abstract no. M.D.114). Unique Identifier : AIDSLINE ICA7/1011491; Chang SW; Arthur J; Hirozawa A; Franks D; Lemp G; Department of Public Health, San Francisco, CA, USA; OBJECTIVE: To determine the rate and predictors of rehospitalization (rehosp) among persons with AIDS (PWAs) discharged from SF hospitals. METHODS: Discharge information from nine San Francisco acute care hospitals was linked with AIDS surveillance data to evaluate hospitalizations (hosp) occurring from 4/1/90 through
Influence of severity of AIDS complications on health care.: Int Conf AIDS. 1991 Jun 16-21;7(1):52 (abstract no. M.D.115). Unique Identifier : AIDSLINE ICA7/1011591; Markson LE; McKee L; Fanning T; Turner BJ; Center for Research in Medical Education and Health Care,; Jefferson Medical College, Philadelphia, PA, USA; OBJECTIVE: To determine whether AIDS severity assessed in the first 3 months (mos) of AIDS onset is predictive of subsequent health care expenditures. METHODS: The Severity Index for AIDS Patients (SIAP) is based on a model derived from expert opinion and groups patients into four categories by expected survival accor
1987 volume-caseload relationship for HIV-related Pneumocystis carinii pneumonia (PCP) in New York City (NYC).: Int Conf AIDS. 1991 Jun 16-21;7(1):52 (abstract no. M.D.116). Unique Identifier : AIDSLINE ICA7/1011691; Bennett CL; Adams J; Gertler P; Gilman S; Keesey J; Shapiro M; Brook RH; Park RE; UCLA Medical School; OBJECTIVE: Policymakers are debating whether hospitals with large numbers of patients with AIDS provide better care. Mortality rates decline with caseload for PCP patients in one California study, as well as in many studies of surgical procedures. To extend these results and assess their generalizability, we analyzed
In vitro HIV-1 infection of human bone marrow eosinophils: Int Conf AIDS. 1991 Jun 16-21;7(1):92 (abstract no. M.A.1000). Unique Identifier : AIDSLINE ICA7/1100091; Freedman A; Gibson F; Fleming S; Spry C; Griffin G; St. George's Hospital Medical School, London, UK; OBJECTIVE: To investigate the potential of HIV-1 to infect human eosinophils in vitro. METHODS: Bone marrow aspirates from healthy human volunteers were cultured in medium supplemented with recombinant human Interleukin-5 (IL-5) to promote eosinophil differentiation. After 1-3 weeks cultures were inoculated with cell-
Dual tropism of HIV-1 in chimpanzee lymphocytes and monocytes.: Int Conf AIDS. 1991 Jun 16-21;7(1):92 (abstract no. M.A.1001). Unique Identifier : AIDSLINE ICA7/1100191; Kupcu Z; Mannhalter JW; Schaff S; Eder G; Eibl MM; Immuno AG, Vienna, Austria; OBJECTIVE: To study the cellular tropism of HIV-1/IIIB in the chimpanzee. METHODS: Monocytes were prepared from peripheral blood of chimpanzees by adherence to plastic. Prior to infection with HIV-1 strain IIIB the adherent cells were cultured for 7 days in medium containing M-CSF (100 U/ml) and GM-CSF (40 U/ml). Nona
FcRII also mediates HIV entry into CD4 negative macrophages.: Int Conf AIDS. 1991 Jun 16-21;7(1):92 (abstract no. M.A.1002). Unique Identifier : AIDSLINE ICA7/1100291; Nagy K; Dobronyi I; Timar I; Institute of Isotopes, Hungarian Academy of Sciences and; Department of Pathology, Semmelweis Medical University, Budapest,; Hungary; OBJECTIVE: To evaluate the role of FcRII(CD32) of CD4 negative human machrophage cells in receptor mediated enhancement of HIV infection. METHODS: Membrane markers characterisation of the machrophes cell line DD were done by immunofluorescence (IF), DD cells infected with HIV directly or complexed with HIV-specific an
Role of the CDR3 region of CD4 in HIV-1 infection and cytopathogenesis defined by human- and chimpanzee-based CD4 synthetic peptides.: Int Conf AIDS. 1991 Jun 16-21;7(1):92 (abstract no. M.A.1003). Unique Identifier : AIDSLINE ICA7/1100391; Eiden LE; Rausch DM; Kalyanaraman VS; Hwang KM; Berger EA; Lifson JD; NIMH, Bethesda, Maryland, USA; OBJECTIVE: Peptide derivatives of CD4 (81-92), a region which includes a portion of the CDR3 domain important in gp 120 binding to CD4, were synthesized, based on either the chimpanzee or human sequence. Differential ability of the two peptides to inhibit gp120 binding to CD4, HIV infection, or cell fusion could help
Association of a kinase activity with the CD4 molecule expressed by human myeloid cell lines and macrophages, and modulation of CD4 expression and the CD4-kinase association by cytokines.: Int Conf AIDS. 1991 Jun 16-21;7(1):93 (abstract no. M.A.1004). Unique Identifier : AIDSLINE ICA7/1100491; Barton G; Sorio C; Cassatella MA; Rossi F; Institute of General Pathology, University of Verona, Italy; OBJECTIVE: Since the significance of CD4 expression by human mononuclear phagocytes is largely unknown, we initiated studies aimed to establish whether CD4 expressed by human myeloid cell lines and macrophages is able to deliver a signal. Furthermore we have investigated regulation of CD4 expression on human myeloid c
Binding of HIV-1 gp120 protein to CD4-negative colon cancer cell lines.: Int Conf AIDS. 1991 Jun 16-21;7(1):93 (abstract no. M.A.1005). Unique Identifier : AIDSLINE ICA7/1100591; Heyworth MF; Sullivan KT; Liu GH; Kim YS; VA Medical Center and University of California, San Francisco,; CA, USA; OBJECTIVE: The aims of this work were (1) to label recombinant gp120 (surface glycoprotein of HIV-1) with biotin, and (2) to compare the ability of biotinylated gp120 to bind to human lymphocytes and colon cancer cell lines. METHODS: Recombinant glycosylated gp120 was labeled with biotin, by using sulfosuccinimidyl 6-
Dual tropism for lymphocytes and macrophages is a common feature of all HIV isolates.: Int Conf AIDS. 1991 Jun 16-21;7(1):93 (abstract no. M.A.1006). Unique Identifier : AIDSLINE ICA7/1100691; Valentin A; Fredriksson R; Albert J; Fenyo EM; Asjo B; Depts. of Virology, Karolinska Institute and National; Bacteriological Laboratory, Stockholm, Sweden; AIM OF THE STUDY: The aim of the study was to investigate whether HIV isolates show any difference in the capacity to infect and replicate in peripheral blood leukocytes (PBL) and blood derived macrophages (BDM). MATERIAL AND METHODS: PBL and BDM obtained from HIV seronegative healthy blood donors were infected with 5
HIV-1 gp120 and full length (FL) gp160 can bind to cells by different mechanisms.: Int Conf AIDS. 1991 Jun 16-21;7(1):93 (abstract no. M.A.1007). Unique Identifier : AIDSLINE ICA7/1100791; Kelker HC; Georgescu R; Valentine FT; NYU Medical Center, New York, NY 10016; OBJECTIVE: To compare the binding of several recombinant gp120s and gp160s to CD4 in an ELISA and a cellular system. METHODS: We have examined the binding of env proteins to: 1) solid phase CD4 (Biogen) using an ELISA assay which we have developed, and 2) CD4+ cells (flow cytometry). Binding of gp120 produced by Ameri
Antibody dependent enhancement of HIV-1 infection requires CD4 receptor and FcR or II.: Int Conf AIDS. 1991 Jun 16-21;7(1):94 (abstract no. M.A.1008). Unique Identifier : AIDSLINE ICA7/1100891; Kobayashi K; Takeda A; Fanger MW; Ennis FA; Division of Infectious Diseases and Immunology, University of; Massachusetts, Worcester, MA; OBJECTIVE: We previously reported that the sera of HIV-1 infected patients have antibodies which enhance infection by HIV-1 of U937 cells and human monocytes (Takeda, A., et al, Science, 1988). Antibody dependent enhancement (ADE) required interaction with both CD4 and Fcalpha receptor (FcalphaR) I (Takeda, A. et al,
Analysis of HIV infections in CD4 mutants of human macrophage-like cell lines.: Int Conf AIDS. 1991 Jun 16-21;7(1):94 (abstract no. M.A.1009). Unique Identifier : AIDSLINE ICA7/1100991; Brys L; Verhaegen S; Saman E; Fransen L; van der Groen G; Van Heuverswyn H; De Baetselier P; Institute of Tropical Medicine - Antwerpen - Belgium; OBJECTIVE: To analyze the role of CD4 in the infection and production of HTLVIIIB in human macrophage-like cell lines, CD4 mutants were generated and tested for their susceptibility towards productive HTLVIIIB infections. METHODS: U937 and THP-1 cells were treated with mutagens and selected for the absence of CD4 via
Expression and localization of CD4 in human fetal neurons and glial cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):94 (abstract no. M.A.1010). Unique Identifier : AIDSLINE ICA7/1101091; Torelli S; Ennas MG; Sogos V; Cocchia D; Gremo F; School of Medicine, Cagliari, Italy; Infants with AIDS present severe neurological symptoms, which could be either a consequence of the general disease or the product of a direct infection of the brain occurring during pregnancy. So far, no direct evidence has been provided about the presence of the cellular receptor for HIV, CD4, in human fetal brain. W
Lectin-carbohydrate interactions and infectivity of HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):94 (abstract no. M.A.1011). Unique Identifier : AIDSLINE ICA7/1101191; Gattegno L; Ramdani A; Jouault T; Saffar L; Gluckman JC; Faculte de Medecine, Paris-Nord, France; OBJECTIVE: To examine whether mannosyl-specific lectins intervene in rgp160 binding to the cell membrane in a CD4-dependent or- independent manner, and to what extent does lectin-mediated enhanced binding of env glycoprotein correspond to modified susceptibility to HIV-1 infection of monocytic cells as compared with l
HIV infection of bone marrow-derived dendritic cells (DC).: Int Conf AIDS. 1991 Jun 16-21;7(1):95 (abstract no. M.A.1012). Unique Identifier : AIDSLINE ICA7/1101291; Patterson S; Gross J; Bedford P; Knight SC; Clinical Research Centre, Harrow, Middlesex, UK; OBJECTIVE: To characterize different populations of peripheral blood DC and investigate their susceptibility to HIV infection. METHODS: The presence of DR, DQ, CD4, CD3, CD19 and CD14 antigens on DC was assessed by immunogold electron microscopy (EM). Susceptibility to in vitro HIV infection was examined by EM and in
Decreased HIV-1 infectivity after in vitro down-regulation of CD4 with phorbol 12-myristate 13-acetate (PMA).: Int Conf AIDS. 1991 Jun 16-21;7(1):95 (abstract no. M.A.1013). Unique Identifier : AIDSLINE ICA7/1101391; Sanhadji K; Benard I; Touraine JL; Faculte de Medecine A. Carrel, INSERM U.80-CNRS URA 1177, Rue; Guillaume Paradin, 69008 Lyon, France; OBJECTIVE: To analyze the variation of HIV infectivity of lymphocytes when CD4 expression is modulated by in vitro pre-treatment with PMA. METHODS: The MT4 cell line (3x10(5) cells/ml) was pre-treated with 1.6x10(-8) M of PMA (or with control solution) for 0.5, 1, 5 or 20 hrs, then washed and assayed for CD4 expressio
A non-immunoglobulin, normal serum protein binds specifically to the gp41 binding domain of HIV gp120.: Int Conf AIDS. 1991 Jun 16-21;7(1):95 (abstract no. M.A.1014). Unique Identifier : AIDSLINE ICA7/1101491; Pinter C; Siccardi AG; Clivio A; Dipartimento di Biologia e Genetica per le Scienze Mediche,; University of Milan, Italy; OBJECTIVE: To search, in normal human sera, HIV binding proteins possibly involved in HIV spreading strategies such as dissemination, alternative tropism or masking of functionally relevant epitopes at the virion surface. METHODS: Normal human sera were pooled and fractionated by high resolution anion exchange HPLC (H
HIV-1 gp120 mimicks a HLA class I hidden epitope.: Int Conf AIDS. 1991 Jun 16-21;7(1):95 (abstract no. M.A.1015). Unique Identifier : AIDSLINE ICA7/1101591; Meneveri R; Grassi F; Marozzi A; Agresti A; Siccardi A; Ginelli E; Dipartimento Biologia e Genetica per li Scienze Mediche,; Universita di Milano, Italy; OBJECTIVE: To characterize a 45 kD human protein which shares an epitope with HIV-1 gp120. The protein, present at high concentrations on the surface of activated immunocytes, is defined by two Mabs (M38 and L31) one of which (M38) is cross-reactive with the virus. METHODS: Immunoscreening of an expression cDNA librar
Binding of CD4 to synthetic peptides patterned on the principal neutralizing domain of gp120.: Int Conf AIDS. 1991 Jun 16-21;7(1):96 (abstract no. M.A.1016). Unique Identifier : AIDSLINE ICA7/1101691; Autiero M; Abrescia P; Dettin M; DiBello C; Guardiola J; CNR, Naples, Italy; OBJECTIVE: Studies on the binding of gp120 and gp120- derived peptides to Sepharose 4B-linked Soluble CD4 were performed. The synthetic peptides corresponding to the PND of gp120 (aa. 308-331) are able to bind CD4 both as an immobilized recombinant molecule and as a membrane-bound antigen and the interaction is specif
The growth pattern of laboratory HIV-1 strains does not reflect the biological behaviour of the virus in vivo.: Int Conf AIDS. 1991 Jun 16-21;7(1):96 (abstract no. M.A.1017). Unique Identifier : AIDSLINE ICA7/1101791; Lu W; Ferreira N; Israel-Biet D; Andrieu JM; Laboratory of Tumor Immunology, Laennec Hospital, Universite de; Paris V, Paris, France; OBJECTIVE: Given the importance of a precise knowledge of HIV tropism and growth characteristics for a better understanding of the HIV disease, we have undertaken a study of the infectivity, growth kinetics, and cytopathogenic effect of laboratory adapted HIV-1 strains, and compared them with those of fresh primary HI
TGF-beta overcomes the lymphocytotropic restriction of HIV-1: Int Conf AIDS. 1991 Jun 16-21;7(1):96 (abstract no. M.A.1018). Unique Identifier : AIDSLINE ICA7/1101891; Lazdins J; Alteri E; Woods-Cook K; Klimkait T; Cox D; Bilbe G; Cerletti N; McMaster G; CIBA-GEIGY Ltd. Basel CH 4002; OBJECTIVE: Evaluation of the role of TGF-Beta on HIV-1 replication in blood derived macrophages (M0). METHODS: Monocytes were isolated by leukapheresis and purified by elutriation. After differentiation M0 were infected with HIV-1 ADA (monocytotropic) IIIB, LAV or Z-84 (lymphocytotropic). Infection was evaluated by su
Interaction of HIV-1 with human endothelial cells expressing a transfected CD4 gene.: Int Conf AIDS. 1991 Jun 16-21;7(1):96 (abstract no. M.A.1019). Unique Identifier : AIDSLINE ICA7/1101991; Hurwitz AA; Hatch WC; Lyman WD; Hatcher VB; Berman JW; Albert Einstein Coll. Med., NY, NY, USA; OBJECTIVE: To test the hypothesis that endothelial cells can be infected by HIV-1. METHODS: Early passage human umbilical vein endothelial (HUVE) cells were transfected with a full-length CD4 cDNA and a selectable neomycin-resistance marker (CD4+EC). CD4+EC and nontransfected control HUVE were tested for CD4 expressio
HIV-1 infection of human fetal macrophages in vivo and in vitro: kinetics of viral gene expression in culture.: Int Conf AIDS. 1991 Jun 16-21;7(1):97 (abstract no. M.A.1020). Unique Identifier : AIDSLINE ICA7/1102091; Hatch WC; Burstein Y; Calvelli TA; Rashbaum WK; Lyman WD; Albert Einstein Coll. Med., NY, NY, USA; OBJECTIVE: To compare the kinetics of HIV-1 gene expression in human fetal macrophages (HFM) infected in vivo with the kinetics of infection of normal HFM in vitro using different HIV-1 isolates. METHODS: HFM from fetal livers were obtained from abortuses of HIV-1 seropositive and seronegative women. The cells were cu
Potential role for complement receptor 2 (CR2) in complement (C) mediated enhancement of HIV in vitro and in vivo.: Int Conf AIDS. 1991 Jun 16-21;7(1):97 (abstract no. M.A.1021). Unique Identifier : AIDSLINE ICA7/1102191; June R; Spear G; Stefanik K; Lint T; Landay AL; Rush Presbyterian St. Luke's Medical Center, Chicago, Illinois,; USA; OBJECTIVE: This study was designed to determine the mechanism of complement mediated enhancement of HIV-1 infection by monitoring provirus formation and virus binding. It also characterized a population of T lymphocytes in vivo which expressed the receptors required for enhancement. METHODS: Supernatants containing HI
Kinetics of HIV-1 fusion with liposomes and erythrocyte ghosts.: Int Conf AIDS. 1991 Jun 16-21;7(1):97 (abstract no. M.A.1022). Unique Identifier : AIDSLINE ICA7/1102291; Larsen CE; Nir S; Alford DR; Jennings M; Lee KD; Duzgunes N; University of California, San Francisco, CA, USA; OBJECTIVE: We investigated the kinetics and extent of membrane fusion between HIV-1 and phospholipid vesicles and erythrocyte ghost membranes and studied the effects of pH, divalent cation, target membrane composition, temperature and viral trypsinization on this process. METHODS: Purified HIV-1 was labeled with octad
Use of a rapid CD4-based ELISA to measure binding of variant gp120 molecules and to screen for inhibitors of binding.: Int Conf AIDS. 1991 Jun 16-21;7(1):97 (abstract no. M.A.1023). Unique Identifier : AIDSLINE ICA7/1102391; Brigido LF; Gilbert M; Culp J; Mills J; SFGH-University of California, San Francisco, CA; OBJECTIVE: To develop a simple, rapid and economical system to measure the binding of variant gp120 molecules to rsCD4, and to screen for inhibitors of binding potentially suitable as antivirals. METHODS: An ELISA previously described (Gilbert et al, J. Clin. Micro 29:142-147;1991) was modified for these purposes. 10
Complement mediates HIV-1 infection of human cells in an antibody-independent fashion.: Int Conf AIDS. 1991 Jun 16-21;7(1):98 (abstract no. M.A.1024). Unique Identifier : AIDSLINE ICA7/1102491; Boyer V; Trabaud MA; Noraz N; Fischer E; Kazatchkine M; Desgranges C; Unite de recherche sur les Hepatites, la SIDA, et les; retrovirus humains. INSERM U271.151, Cours A. Thomas, Lyon.; OBJECTIVE: To investigate the role of complement in HIV-1 infection of different types of cells independently of the HIV specific antibodies. METHODS: To determine the role of complement in HIV-1 infection we incubated the HTLV-IIIB and HTLV-RF strains of HIV-1 with seronegative normal human serum under conditions whi
Genomic variability of HTLV-1 according to geographical areas and associated pathology.: Int Conf AIDS. 1991 Jun 16-21;7(1):98 (abstract no. M.A.1025). Unique Identifier : AIDSLINE ICA7/1102591; Komurian F; Pelloquin F; Giordano C; Gessain A; Sonoda S; de-The G; Merieux Institute, Marcy l'Etoile, France; In order to study the geographical and disease-associated variations of the HTLV-I genome, the ex-vivo DNA from nine patients were studied by PCR, followed by direct DNA sequencing. For each viral strain, about 2,000 bp were thus sequenced, including LTR, env gene, px II, px III and px IV coding frames of the px regio
HIV envelope chimeric proteins for retroviral targeting.: Int Conf AIDS. 1991 Jun 16-21;7(1):98 (abstract no. M.A.1026). Unique Identifier : AIDSLINE ICA7/1102691; Levy DN; Williams WV; Weiner DB; University of Pennsylvania, Philadelphia, Pennsylvania, USA; OBJECTIVE: The cellular tropism of HIV is determined by the envelope proteins. The precursor glycoprotein gp160 is post-translationally cleaved to yield gp120 and gp41. The external glycoprotein gp120 is noncovalently attached to gp41 and mediates virus binding to the T cell antigen CD4. The transmembrane glycoprotein
The establishment of a rat cell line continuously producing HIV1.: Int Conf AIDS. 1991 Jun 16-21;7(1):98 (abstract no. M.A.1027). Unique Identifier : AIDSLINE ICA7/1102791; Mizrachi Y; Sternas L; Volsky DJ; St. Luke's/Roosevelt Hospital Center, Columbia University, New; York, N.Y.; OBJECTIVE: To establish a rodent cell line stably carrying HIV-1 genome and replicating HIV-1 over extended periods of time, as a model system for studying HIV-1 replication in the absence of HIV-1 receptors. METHODS: A vector carrying an infectious clone of HIV-1 and the neomycin resistance gene was electroporated in
Complement activation by gp160 and complement-mediated enhancement of HIV infection of monocytic cell lines.: Int Conf AIDS. 1991 Jun 16-21;7(1):99 (abstract no. M.A.1028). Unique Identifier : AIDSLINE ICA7/1102891; Haeffner-Cavaillon N; Thieblemont N; Weiss L; Ziegler-Heitbrock HW; Kazatchkine MD; Hopital Brousais, Paris, France; OBJECTIVE: To study the role of complement in the facilitation of viral entry into monocyte/macrophages. METHODS: Glycosylated recombinant gp160 of HIV-1; monocytic cell lines Mono Mac 6 (CD4 antigen-negative) and THP1 (low expression of CD4 antigen); HTLV IIIB strain of HIV-1; LAV II strain of HIV-2. RESULTS: Incubat
Monoclonal antibodies directed against synthetic CD4 peptides.: Int Conf AIDS. 1991 Jun 16-21;7(1):99 (abstract no. M.A.1029). Unique Identifier : AIDSLINE ICA7/1102991; Maino V; Singer R; Buck D; Buchanan T; Becton Dickinson Immunocytometry Systems, San Jose, CA; OBJECTIVE: Synthetic peptides derived from CD4 sequences of the putative binding domain of gp120 were employed in an effort to further characterize the gp120 binding site on CD4. METHODS: Monoclonal antibodies (MAb) raised against synthetic peptides derived from sequences of the V1 domain were evaluated for their abil
Development of an HIV-1 infection system for studies on viral life cycle in neural cells and isolation of neurotropic HIV-1 strains.: Int Conf AIDS. 1991 Jun 16-21;7(1):99 (abstract no. M.A.1030). Unique Identifier : AIDSLINE ICA7/1103091; Volsky B; Sakai K; Shahabuddin M; Volsky DJ; Molecular Virology Laboratory, St.Luke's/Roosevelt Hospital; Center, Columbia University, New York, N.Y.; OBJECTIVE: HIV-1 infects neural cells but major differences exist compared to its infection of T cells. The route of HIV-1 entry into neural cells is unknown, the infection is small in extent and non-cytopathic, and virus can persist in a dormant/inducible state. Our objective was to develop an in vitro neural cell sy
Correlation between fusion, entry and expression of HIV-1 in human and mouse cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):99 (abstract no. M.A.1031). Unique Identifier : AIDSLINE ICA7/1103191; Zeira M; Littman D; Volsky DJ; Molecular Virology Laboratory, St. Luke's/Roosevelt Hospital; Center, Columbia University, New York, N.Y.; OBJECTIVE: To establish parameters of HIV-1 entry into cells by measuring fusion between viral envelopes and CD4+ T cell lines or freshly isolated PBLs, and to correlate between viral entry and expression in these cells and in mouse cells expressing human CD4 receptors. METHODS: Sucrose gradient purified HIV-1 CAT vir
The comparative toxicokinetics of 3' -deoxy 3' flourothymidine (FLT) in laboratory animals.: Int Conf AIDS. 1991 Jun 16-21;7(1):100 (abstract no. M.A.1032). Unique Identifier : AIDSLINE ICA7/1103291; Johnson D; Dougherty WJ; Pool W; Riley MG; Yacobi A; American Cyanamid Company, Pearl River, New York, USA; OBJECTIVE: To investigate interspecies differences in the toxicokinetics of FLT. METHODS: The comparative toxicokinetics of FLT in the rat and dog was evaluated in studies supported by pharmacokinetics, hematology and pathology. In monkeys, a study focusing on hematology and pharmacokinetics was conducted. RESULTS: In
Viral and cellular requirements for replication of HIV in primary monocytes.: Int Conf AIDS. 1991 Jun 16-21;7(1):100 (abstract no. M.A.1033). Unique Identifier : AIDSLINE ICA7/1103391; Schuitemaker H; Kootstra NA; Groenink M; Meyaard L; de Goede RE; Huisman H; Miedema F; Tersmette M; Central Lab. Neth. Red Cross Blood Transf. Serv. and Lab. of Exp.; and Clin. Immunology of the Univ. of Amsterdam, Amsterdam, The; Netherlands; The biological phenotype and the prevalence of monocytotropic HIV-1 isolates was studied by cell-free infection and primary isolation studies on sequential blood samples. In cell-free infection studies on primary monocyte derived macrophages (MDM) with 17 non-syncytium-inducing (NSI) and 14 syncytium-inducing (SI) iso
A pre-clinical in vitro toxicological model using human lymphocytes.: Int Conf AIDS. 1991 Jun 16-21;7(1):100 (abstract no. M.A.1034). Unique Identifier : AIDSLINE ICA7/1103491; Foster BC; Wilson DL; Tryphonas H; Whitehouse LW; Khan SR; Health & Welfare Canada, Health Protection Branch, Ottawa,; Ontario, Canada; OBJECTIVE: The purpose of this study was to determine whether transformed and peripheral blood leukocytes could be used in a pre-clinical in vitro lymphocyte proliferation assay (3H-thymidine uptake) to determine the potential toxicity of AIDS drugs and whether there would be drug interactions. METHODS: Epstein-Barr v
Preferential susceptibilities of adherent cells derived from human brains to an HIV-1 mutant and HIV-2 and SIV isolates.: Int Conf AIDS. 1991 Jun 16-21;7(1):100 (abstract no. M.A.1035). Unique Identifier : AIDSLINE ICA7/1103591; Takeuchi Y; Hoshino H; Miura T; Hayami M; Clapham PR; Weiss RA; Gunma University, Maebashi, Gunma, Japan; We have been studying a mutant HIV-1, which is named GUN-1V and can productively infect fibroblastoid cells (BT cells) derived from human brains by a CD4-mediated mechanism. BT cells seem to originate from normal blood vessel cells. Proto-type HIV-1 isolates cannot replicate well in BT cells. The genetic difference in
Induction of cytotoxic T lymphocytes against HIV-2 in mice immunized with recombinant salmonella typhimurium.: Int Conf AIDS. 1991 Jun 16-21;7(1):101 (abstract no. M.A.1036). Unique Identifier : AIDSLINE ICA7/1103691; Aggarwal A; Sadoff J; Markham P; Gallo R; Franchini G; PRI/DynCorp, NCI-FCRDC, Frederick, MD, USA; OBJECTIVE: Salmonella-based oral vaccines have shown to be safe, highly immunogenic, and cost effective. Recombinant Salmonella typhimurium (WR4024) expressing HIV-2 antigens could induce viral-specific cytotoxic T lymphocytes (CTLs) in mice. METHODS: PCR-amplified HIV-2 gag (p17 and p24) and env (amino and carboxy ha
HIV envelope glycoprotein gp120 interacts with astroglial beta-adrenergic receptors.: Int Conf AIDS. 1991 Jun 16-21;7(1):101 (abstract no. M.A.1037). Unique Identifier : AIDSLINE ICA7/1103791; Giulio L; Petrucci T; Patrizio M; Bernardo A; Istituto Superiore di Santia, Pathophysiol, and Cell Biol. Labs,; Roma, Italy; OBJECTIVE: It has been hypothesized that neural cell damage responsible for the AIDS dementia complex may result from CD4-independent infection, from release of neurotoxic viral-coded products (e.g. gp120) or from exposure to products (cytokines or others) derived from infected or
Protein gp120 enhances glutamate toxicity in cultures of rat cerebellar interneurons.: Int Conf AIDS. 1991 Jun 16-21;7(1):101 (abstract no. M.A.1038). Unique Identifier : AIDSLINE ICA7/1103891; Tiziana S; Levi G; Instituto Superiore di Sanita, Laboratory of Pathophysiology,; Roma, Italy; OBJECTIVE: It has been suggested that neural cell damage frequently observed in AIDS patients may be partly related to the neurotoxicity of HIV-coded products, such as the envelope protein gp120. Picomolar concentrations of gp120 cause neuronal death in various non-human neural cell culture systems, and this death has
Cytotoxic activity and HIV sensitivity of intestinal lamina propria lymphocytes.: Int Conf AIDS. 1991 Jun 16-21;7(1):101 (abstract no. M.A.1039). Unique Identifier : AIDSLINE ICA7/1103991; DiMassimo AM; Placido R; Bach S; Mastino AS; Capobianchi M; Fais S; Pallone F; Colizzi Y; Department of Biology and of Experimental Medicines, II; University of Rome, Italy; OBJECTIVE: The role of mucosal immunity in HIV infection has been analyzed at the level of lamina propria lymphocytes (LPL). METHODS: LPL were isolated from the normal colon-rectal mucosa by enzymatic treatment, and phenotypically characterized by flow cytometry. T cell mitogen (PHA, staphylococcus toxin,
Human diploid fibroblasts and epithelial cells pre-treated with interferon gamma present increased HIV adsorption.: Int Conf AIDS. 1991 Jun 16-21;7(1):102 (abstract no. M.A.1040). Unique Identifier : AIDSLINE ICA7/1104091; Dolei A; Serra C; Arca MV; Toniolo A; Institute of Microbiology and Virology, University of Sassari,; Italy; OBJECTIVE: To correlate the permissiveness to HIV of adherent cells to the expression of surface molecules. METHODS: HIV-1 (strain HTLV-IIIB) was given to human diploid lung embryo fibroblasts and to other adherent human cell lines, either untreated or pre-treated with recombinant interferon (IFN) gamma. Modulation oft
In vitro bone marrow colony assays and long-term bone marrow culture in HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):102 (abstract no. M.A.1041). Unique Identifier : AIDSLINE ICA7/1104191; Kaczmarski RS; Sutherland S; McManus T; Moxham J; Moran S; Mufti GJ; King's College Hospital School of Medicine and Dentistry, London,; England; OBJECTIVES: The pathogenesis of peripheral blood cytopenias in HIV infection is likely to be multifactorial. Proposed mechanisms include direct infection of haemopoietic cells or progenitors, stromal dysregulation of haemopoiesis, immune-mediated destruction and the myelosuppressive effects of drugs. Bone marrow abnort
A cell line susceptible to a selectively macrophage-tropic HIV-1 strain (BaL0): lack of cytophaticity, CD4 downregulation and receptor interference.: Int Conf AIDS. 1991 Jun 16-21;7(1):102 (abstract no. M.A.1042). Unique Identifier : AIDSLINE ICA7/1104291; Balotta C; Veronese F; Lori F; Pal R; Gallo RC; Lusso P; Laboratory of Tumor Cell Biology, NCI, Bethesda, MD 20892; OBJECTIVE: To establish a unified model for the study of the biology of divergent HIV isolates. METHODS: Syncytia assay, p24 Ag capture, flow cytometry, sCD4 competiton assay. RESULTS: From the HIV-1BaL isolate, we obtained a biologically pure macrophage-tropic substrain, HIV-1BaL0, which is unable to penetrate PHA-act
Mechanisms of ganglioside-induced modulation of CD4 molecule: Int Conf AIDS. 1991 Jun 16-21;7(1):102 (abstract no. M.A.1043). Unique Identifier : AIDSLINE ICA7/1104391; Saggioro D; Panozzo M; Calabro ML; Callegaro L; Chieco-Bianchi L; University of Padova, Italy; OBJECTIVE: To study the mechanism of monoganglioside GM1 induced CD4 down regulation in CD4 positive cells. METHODS: 1) CD4 down-modulation was measured by flow cytometric analysis in T cell lines and in HeLa cells expressing a CD4 molecule defective for the cytoplasmic region; 2) HIV-infection in GM1-treated cells wat
HIV infection of a cell line derived from novel gastrointestinal T-lymphocyte population (CD4-, CD8-, TCR alpha beta +).: Int Conf AIDS. 1991 Jun 16-21;7(1):103 (abstract no. M.A.1045). Unique Identifier : AIDSLINE ICA7/1104591; Toshio H; Maeda Y; Maeda M; Takatsuki K; Kumamoto University Medical School, Kumamoto, Japan; OBJECTIVE: Dysfunctions of local immunity are presumed to be prerequisite for systemic immunodeficiency. To obtain clues for the mechanisms of immunodeficient states in HIV infected individuals, we have asked whether a T cell line derived from novel T-lymphocyte population in the gastrointestinal tract could be infectt
Production of adherent cell lines susceptible to lymphotropic viruses.: Int Conf AIDS. 1991 Jun 16-21;7(1):103 (abstract no. M.A.1046). Unique Identifier : AIDSLINE ICA7/1104691; Toniolo A; Serra C; Arca MV; Babudieri S; Dolei A; Dpt. of Biomedicine, University of Pisa, Italy; OBJECTIVE: Adherent cells capable of expressing selected leukocyte markers were produced to analyze the relationships between surface receptor molecules and susceptibility to some lymphotropic agents. METHODS: Somatic hybridization of human leukocytes with human epithelial cell lines (together with immunoselection) alt
Ability of antisera raised to synthetic peptides representing the V3 region of different HIV-1 isolates to neutralize infection of both primary monocytes/macrophages and T-cell lines.: Int Conf AIDS. 1991 Jun 16-21;7(1):103 (abstract no. M.A.1047). Unique Identifier : AIDSLINE ICA7/1104791; Butto S; McDanal CB; Greenwell TK; Streilein AF; Langlois AJ; Bolognesi DP; Matthews TJ; Department of Surgery, Duke University Medical Center, Durham, NC; 22710; The pathogenesis of AIDS remains incompletely understood. Although HIV causes a cytocidal infection of CD-4 bearing cells in vitro, this phenomenon alone is insufficient to fully explain the immunologic dysfunction associated with AIDS. In particular, the role of monocytes/macrophages is poorly understood. OBJECTIVE: t
Persistent infection of a human neuronal cell line by HIV-1: virus-cell interaction.: Int Conf AIDS. 1991 Jun 16-21;7(1):104 (abstract no. M.A.1048). Unique Identifier : AIDSLINE ICA7/1104891; Orecchia A; Equestre M; Carlini F; Novello F; Federico M; Santoro R; Genovese D; Lab. Virology, Istituto Superiore di Sanita, Roma, Italy; OBJECTIVE: To study the mechanism of interaction between HIV-1 and Central Nervous System (CNS) cells. Dysfunction of the CNS is a prominent feature of the AIDS syndrome. The precise role of the HIV in CNS dysfunction remains to be determined. For this purpose an in vitro model was established to study the mechanism ot
Soluble CD4 (sCD4) suppress the antigen-driven proliferative response in certain BCG specific T-cell clones.: Int Conf AIDS. 1991 Jun 16-21;7(1):104 (abstract no. M.A.1049). Unique Identifier : AIDSLINE ICA7/1104991; Magnus G; Halapi E; Wigzell H; Kiessling R; Department of Immunology, Karolinska Institute, Stockholm, Sweden; We have investigated the effect of soluble recombinant CD4 (sCD4) on the antigen (BCG) specific response of polyclonal T-cell lines or on T-cell clones derived from these. Some of the antigen specific clones could be suppressed in their antigen driven response by the addition of sCD4, while others, including the parent
HIV infection of monocytes self-perpetuates HIV enhancer activity through NF-kB induction.: Int Conf AIDS. 1991 Jun 16-21;7(1):104 (abstract no. M.A.1050). Unique Identifier : AIDSLINE ICA7/1105091; Alcami J; Bachelerie F; Arenzana-Seisdedos F; Virelizier JL; Unite d'Immunologie Virale, Institut Pasteur, Paris; OBJECTIVE: Tissue macrophages are probably the main reservoir for HIV in vivo. Chronic HIV replication in the macrophage lineage would be expected to result from the constitutive presence of nuclear NF-kB, the transcriptional activator of the HIV enhancer. However, in U937 cells, a myelomonocytic line highly permissivt
Differential HIV-1 replication in naive and memory CD4 T-cells from HIV-patients according to activation pathway employed.: Int Conf AIDS. 1991 Jun 16-21;7(1):104 (abstract no. M.A.1051). Unique Identifier : AIDSLINE ICA7/1105191; Cayota A; Vuillier F; Scott-Algara D; Feuillie V; Dighiero G; Unite d'Immunohematologie et d'Immunopathologie, Institut; Pasteur, Paris, France; To study HIV replication in naive and memory CD4 T-cells, as well as, the role of different stimulatory pathways on viral replication, peripheral blood mononuclear cells (PBMCs) from 8 HIV-1-seropositive carriers (SPC) and 4 AIDS patients were positively selected into CD4+CD45RA+ and CD4+CD45RA- subsets using an EPICSt
Control of viral replication in mononuclear cells of HIV-antibody-positive and HIV-antibody-negative hemophiliacs.: Int Conf AIDS. 1991 Jun 16-21;7(1):105 (abstract no. M.A.1052). Unique Identifier : AIDSLINE ICA7/1105291; Mihoczy E; Mannhalter JW; Schramm W; Eibl MM; Immuno AG, Vienna, Austria; OBJECTIVE: To compare viral replication in mononuclear cells (MNC) of HIV-antibody-positive (HIV+) and HIV-antibody-negative (HIV-) hemophilia patients (HP) after in vitro infection with HIV-1 IIIB. METHODS: Long-term cultures of HIV-1 IIIB-infected PHA blasts were set up in IL-2-containing medium. Virus released intot
Production of HIV-1 protein but not infectious virus in G1 arrested MT-2 cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):105 (abstract no. M.A.1053). Unique Identifier : AIDSLINE ICA7/1105391; Kiernan R; Doherty RR; McPhee D; Macfarlane Burnet Centre for Medical Research, Fairfield; Hospital, Victoria, Australia; OBJECTIVE: To investigate the mechanism of inhibition of HIV replication in G1 (stationary phase) arrested HTLV-1 transformed MT-2 cells. METHODS: Cells were arrested in G1 phase using 5mM hydroxyurea, shown by FACS analysis of DNA content & greater than 90% reduction of (3)H-TTP uptake. After 18 h pretreatment wit
Inhibition of HIV-reverse transcriptase by synthetic oligonucleotides.: Int Conf AIDS. 1991 Jun 16-21;7(1):105 (abstract no. M.A.1054). Unique Identifier : AIDSLINE ICA7/1105491; Idriss H; Stammers DK; Wellcome Research Laboratories, Dept. of Molecular Sciences,; South Eden Park Road, Beckenham, Kent, England; OBJECTIVE: The reverse transcriptase (RT) of the HIV virus is an important target for anti-HIV drugs such as azidothymidine (Zidovudine). In addition to the RNA dependent DNA polymerase activity, RT also catalyse a DNA dependent DNA polymerase activity as a step in the formation of pro-viral DNA. The substrate activit
Characterization of a eucaryotic form of the HIV-1 reverse transcriptase.: Int Conf AIDS. 1991 Jun 16-21;7(1):105 (abstract no. M.A.1055). Unique Identifier : AIDSLINE ICA7/1105591; West A; Roberts TM; Kolodner RD; Harvard Medical School, Dana-Farber Cancer Institute, Boston,; Massachusetts, USA; OBJECTIVE: Examination of the HIV reverse transcriptase with respect to subunit structure, kinetics and processivity. METHODS: The baculoviral expression system was used to make large quantities of the products of the pol open reading frame. Monoclonal antibodies were made against the reverse transcriptase and an immu
Activation of Human Immunodeficiency Virus-1 (HIV-1) by DNA demethylation.: Int Conf AIDS. 1991 Jun 16-21;7(1):106 (abstract no. M.A.1056). Unique Identifier : AIDSLINE ICA7/1105691; O'Brien MC; Mitsuya H; The Clinical Oncology Program, National Cancer Institute,; National Institutes of Health, Bethesda, Maryland, USA; OBJECTIVES: To study the effect of DNA demethylation on HIV expression in various infected cells. METHODS: 5-Azacytidine (AZC) and its analog (dihydro-5-azacytidine, DHAC) were employed as agents that induced DNA demethylation. An HIV-infected T-cell line, ACH2, that produces a low level of HIV-1; a persistently HIV-1
Characterization of HIV-1 reverse transcriptase using a set of 23 murine monoclonal antibodies.: Int Conf AIDS. 1991 Jun 16-21;7(1):106 (abstract no. M.A.1057). Unique Identifier : AIDSLINE ICA7/1105791; Restle T; Sczakiel G; Muller B; Pawlita M; Goody RS; Max Planck Institut fur med. Forschung, Abtellung Biophysik,; Heidelberg, FRG; OBJECTIVE: Reverse transcriptases (RT) are interesting targets for chemotherapeutic treatment of retroviral diseases. A detailed knowledge of the correlation between structure and function of this enzyme family is the prerequisite for a rational drug design. Due to the lack of suitable crystals for X-ray diffraction a
Expression of HIV-2 reverse transcriptase in E. coli and characterization of the recombinant enzyme.: Int Conf AIDS. 1991 Jun 16-21;7(1):106 (abstract no. M.A.1058). Unique Identifier : AIDSLINE ICA7/1105891; Muller B; Restle H; Kuhnel H; Goody RS; Abt. Biophysik, Max-Planck-Institut fur med. Forschung,; Heidelberg, FRG; OBJECTIVE: When studying the structure and function of HIV reverse transcriptase (RT), it is of special interest to compare the enzymes from HIV-1 and HIV-2, which are functionally closely related but show considerable differences in amino acid sequence. Besides comparison of structural and biochemical properties, we
Retinoids stimulate HIV-1 expression in monocytes.: Int Conf AIDS. 1991 Jun 16-21;7(1):106 (abstract no. M.A.1059). Unique Identifier : AIDSLINE ICA7/1105991; Turpin JA; Meltzer MS; Walter Reed Army Institute of Research, Washington, DC, USA; OBJECTIVE: Retinoids are potent mediators of mononuclear phagocyte differentiation. Since vitamin A is a dietary component, the effect of this compound on HIV replication is of interest. METHODS: Blood monocytes were purified from PBMC by countercurrent centrifugal elutriation and cultured in medium with 10% human ser
Differential effects of L-thyroxine and prostaglandins on the tat-mediated transactivation of HIV-1 LTR in CD4 cells and macrophages.: Int Conf AIDS. 1991 Jun 16-21;7(1):107 (abstract no. M.A.1060). Unique Identifier : AIDSLINE ICA7/1106091; Demirhan I; Chandra A; Hofmann D; Chandra P; Laboratory of Molecular Biology (ZBC) Frankfurt University; Medical School, FRG; OBJECTIVE: Transactivation of HIV-1 LTR is the most important event in the expression of structural genes. Our aim is to look for endogenous factors which affect this process in target cells (CD4 cells and macrophages) modulating the pathophysiological state of the disease. METHODS: Jurkat cells or U937 cells were tra
Investigation of the role of DNA methylation in the maintenance of HIV latency in monocytes.: Int Conf AIDS. 1991 Jun 16-21;7(1):107 (abstract no. M.A.1061). Unique Identifier : AIDSLINE ICA7/1106191; Walker MC; Bouchard J; Leclerc JM; Varin M; Patel PC; Institut Armand-Frappier, Laval, Que., Canada; OBJECTIVE: The objective of the study was to examine the possible role of DNA methylation in the maintenance of HIV latency in cells of mononuclear phagocyte lineage. METHODS: U1.1 cells, a clone of the human promonocyte cell line U937, which is chronically infected with the LAV strain of HIV-1, were obtained from T.M
Characterization of RT inhibitory antibodies in sera of HIV-1 infected individuals.: Int Conf AIDS. 1991 Jun 16-21;7(1):107 (abstract no. M.A.1062). Unique Identifier : AIDSLINE ICA7/1106291; DeVico A; Rahman R; Gallo R; Sarngadharan M; di Marzo Veronese F; Dept. of Cell Biology, Advanced BioScience Laboratories, Inc.,; Kensington, MD, USA; OBJECTIVE: We became interested in the identification of the antibody binding domain related to the induction of RT inhibitory antibodies and in the characterization of the mechanism involved in this inhibition as a means of identifying RT domains important for enzymatic activity. METHODS: RT specific immunoglobulins
Determinants of the HIV-1 transmembrane protein necessary for virus entry and syncytia formation.: Int Conf AIDS. 1991 Jun 16-21;7(1):107 (abstract no. M.A.1063). Unique Identifier : AIDSLINE ICA7/1106391; Dedera D; Ratner L; Washington University School of Medicine, St. Louis, MO, USA; OBJECTIVE: This study was performed to define regions of the transmembrane protein (TM) involved in virus entry and syncytia formation. METHODS: Site-directed mutations were made which altered the N-terminal fusion domain (F3, F6), conserved potential N-linked glycosylation sites and cysteine residues. Infectivity, re
A novel, dipyridodiazepinone inhibitor of HIV-1 reverse transcriptase acts through a non-substrate binding site.: Int Conf AIDS. 1991 Jun 16-21;7(1):108 (abstract no. M.A.1064). Unique Identifier : AIDSLINE ICA7/1106491; Wu JC; Warren TC; Adams J; Proudfoot J; Skiles J; Raghavan P; Perry C; Potocki I; Farina PR; Grob PM; Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, CT, USA; OBJECTIVE: A novel, non-nucleoside inhibitor BI-RG-587 inhibits HIV-1 RT (RT-1) with an IC50 = 85 nM. The interaction of this inhibitor with RT-1 was investigated. METHODS: A tritiated azido-analog of BI-RG-587 was synthesized (3[H]-BI-RJ-70) and used to label RT-1 in the presence or absence of dGTP, template-primer,
Cellular DNA synthesis and mitosis are not required for the establishment of infection of T cell line with HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):108 (abstract no. M.A.1065). Unique Identifier : AIDSLINE ICA7/1106591; Handa A; Takeuchi Y; Hoshino H; Gunma University School of Medicine, Maebashi, Gunma, Japan; OBJECTIVE: Upon de novo infection of cells with retroviruses such as murine leukemia viruses or arian sarcoma virus, production of progeny viruses have been detected after cellular DNA synthesis and mitosis. On the other hand, in humans infected with HIV-1, syntheses of viral mRNA or viral proteins have been observed
Differentiation-inducing agents of leukemic cells enhance infection of T-cells with HIV-1 in vitro.: Int Conf AIDS. 1991 Jun 16-21;7(1):108 (abstract no. M.A.1066). Unique Identifier : AIDSLINE ICA7/1106691; Seki J; Yamamoto A; Takeuchi Y; Hoshino H; Gunma University School of Medicine, Department of Hygiene, Japan; OBJECTIVE: Dimethyl sulfoxide (DMSO) has often been used as a solvent of many chemicals to be assayed for anti-HIV-1 activities. As we noticed that DMSO enhanced HIV-1 infection in vitro, we examined effects of DMSO and other so called differentiation-inducing agents on HIV-1 infection of T cells. METHODS: MTT assay w
DNA-binding-capacity of the HIV-1 integrase expressed in the baculovirus-system.: Int Conf AIDS. 1991 Jun 16-21;7(1):108 (abstract no. M.A.1067). Unique Identifier : AIDSLINE ICA7/1106791; Rodner B; Vinga-Martins C; Muller-Lantzsch N; University of Saarland, Dept. Virology, Homburg, FRG; OBJECTIVE: Expression and functional characterisation of different forms of the HIV-1 integrase in the Baculovirus-system. METHODS: The complete gag and pol reading frames and a clone representing the integrase of HIV-1, which is encoded at the 3 part of the pol gene, were expressed in the eucaryotic Baculovirus-syst
Characterization of cell-to-cell spread of HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):109 (abstract no. M.A.1068). Unique Identifier : AIDSLINE ICA7/1106891; Sato H; Martin A; Laboratory of Molecular Microbiology, NIAID, NIH, Bethesda, USA; OBJECTIVE: To compare HIV infections mediated by cell-free virus or cell-to-cell spread. METHODS: We have compared the kinetics of virus replication following infections mediated by cell-free virus or virus producing cells, using Southern, Northern, and Western analyses. We also examined the effects of metabolic inhib
A rapid test system for evaluating effectors of HIV-1 transmission.: Int Conf AIDS. 1991 Jun 16-21;7(1):109 (abstract no. M.A.1069). Unique Identifier : AIDSLINE ICA7/1106991; Geyer S; Epstein J; Food and Drug Administration, Center for Biologics, USA; OBJECTIVE: The purpose of this study was to establish a quantitative in vitro test system for rapidly evaluating the effects of antibodies, drugs and cytokines on transmission of HIV-1. METHODS: Indicator T-cell (H938) and monocyte (U38) lines (Felber and Pavlakis) were used to develop an in vitro test system. These c
Interactions between MAB and some epitopes of HLA-classi-beta2m antigen complex prevent HIV production in PBMC and MT4 cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):109 (abstract no. M.A.1070). Unique Identifier : AIDSLINE ICA7/1107091; Corbeau P; Emiliani S; Olive D; Favero J; Dornand J; Devaux C; Centre de tri des molecules anti-HIV, CRBM, CNRS-INSERM,; Montpellier, France; OBJECTIVE: We have previously shown (1, 2) that monoclonal antibodies (mAb) specific for beta2 microglobulin, can delay HIV cytopathic effect in MT4, an HTLV1 immortalized T cell line, and, viral production by Peripheral Blood Mononuclear Cells (PBMC). The main purpose of the present study was to investigate the effec
In HIV-1-infected cell clones the replication of superinfecting HIV may be blocked at the retrotranscription step.: Int Conf AIDS. 1991 Jun 16-21;7(1):109 (abstract no. M.A.1071). Unique Identifier : AIDSLINE ICA7/1107191; Taddeo B; Federico M; Titti F; Orecchia A; Saggio I; Carlini F; Verani P; Rossi B; Lab. Virology, Istituto Superiore di Sanita, Rome, Italy; OBJECTIVE: To define at which step superinfecting HIV replication is blocked in both producer (D10) and non producer (F12) HIV-1-infected Hut-78 cell clones exhibiting homologous viral interference (Federico et al., AIDS Res Hum Retr, 1989, 5: 385). METHODS: D10 and F12 CD4-down-regulated HIV-1-infected Hut-78 clones
Comparative investigations on HIV-1 ribosomal frameshifting in vivo and in vitro.: Int Conf AIDS. 1991 Jun 16-21;7(1):110 (abstract no. M.A.1072). Unique Identifier : AIDSLINE ICA7/1107291; Reil H; Steinhaus H; Hoosmayer D; Hauser H; Gesellschaft fur Biotechnologische Forschung, Braunschweig, FRG; Ribosomal frameshifting is essential for the expression of the catalytic proteins (Pro, RT, Int) of HIV-1. A defined sequence of the gag-pol overlapping region has been shown to be necessary for this specific process. We have developed an expression system which allows the quantification of frameshifting in vivo and i
HIV-1 replication in HTLV-1 transformed cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):110 (abstract no. M.A.1073). Unique Identifier : AIDSLINE ICA7/1107391; Antonelli G; Turriziani O; Capobianchi MR; Amicucci P; Di Marco P; Guamnu D; Riva E; Dianzani F; Institute of Virology, University La Sapienza, Rome, Italy; OBJECTIVE: To investigate the biological aspects of HIV-1 replication in HTLV-1 transformed cell lines. METHODS: C8166 or MT4 cells were infected with several HIV-1 isolates at high MOI (1-2 TCID50/cell). Progeny virus as well as viral DNA, viral proteins, and % of infected cells were examined after one cycle of repli
HIV proteinase mutant in cell culture.: Int Conf AIDS. 1991 Jun 16-21;7(1):110 (abstract no. M.A.1074). Unique Identifier : AIDSLINE ICA7/1107491; Heindl B; Nitschko H; von der Heim K; Max-von-Pettenkofer Institut, University of Munich, Munich,; Germany; Retroviral maturation requires activity of the virus encoded protease which processes the viral gag and gag-pol protein precursor. The HIV enclosed protease might be a suitable target for anti-viral therapy using inhibitors which block selectively the viral encoded protease but do not impair cellular functions. The pr
Inhibition of HIV-infectivity and replication by alpha-lipoic acid.: Int Conf AIDS. 1991 Jun 16-21;7(1):110 (abstract no. M.A.1075). Unique Identifier : AIDSLINE ICA7/1107591; Baur A; Harrer T; Peukert M; Jahn G; Kalden JR; Fleckenstein B; Institut fur Klinische und Molekulare Virologie, Universitat; Erlangen /Nurnberg, Loschgestr. 7, 8520 Erlangen, FGR; OBJECTIVE: To study the antiviral activity of alpha-lipoic acid, a naturally occurring disulfide compound, that has been applied for years for the treatment of polyneuropathies and hepatic disorders. METHODS: Acutely and persistently infected T-cells were treated with various doses of alpha-lipoic acid. Reduction of v
Biochemical characterization and mutagenesis of HIV-1 endonuclease.: Int Conf AIDS. 1991 Jun 16-21;7(1):111 (abstract no. M.A.1076). Unique Identifier : AIDSLINE ICA7/1107691; Drelich M; Wilhelm R; Mous Jan; F. Hoffman-La Roche Ltd, Basel, Switzerland; OBJECTIVE: The identification of the endonuclease active site of the HIV-1 integration protein (IN) by site-directed mutagenesis. Biochemical characterization and structure/function analysis of the IN endonuclease and integrase activities. Evaluation of active site mutations when introduced into infectious proviral cl
Virus isolation from lymphocytes of anti-HIV(+) persons: identification of a virus with unusual morphology.: Int Conf AIDS. 1991 Jun 16-21;7(1):111 (abstract no. M.A.1077). Unique Identifier : AIDSLINE ICA7/1107791; Kozlov AP; Glebov AV; Traugott MN; Nikolaeva EV; Skadova VM; Malykh AG; Kurbatova TV; AIDS Reference and Research Lab, Leningrad, 193167, USSR; OBJECTIVE: To study viruses infecting anti-HIV(+) persons in Lenigrad area. METHODS: PBL isolated by Ficoll technique were incubated in presence of PHA and IL-2 . HIV presence was shown by antigen detection (ELISA) and electron microscopy. Viral nucleic acids were detec
A novel polymerase chain reaction for HIV.: Int Conf AIDS. 1991 Jun 16-21;7(1):111 (abstract no. M.A.1078). Unique Identifier : AIDSLINE ICA7/1107891; Bawa N; Manjunath N; Shankar P; Rajamouli P; Broor S; Seth P; All India Institute of Medical Sciences, New Delhi, India; OBJECTIVE: To develop a simplified non radio-isotopic Polymerase Chain Reaction (PCR) and to use this method on HIV isolates from India . METHODS: PCR was done using digoxigenin labeled uridine triphosphate in the reaction mixture, so that it could get incorporated in the amplified pe
A new high-sensitive immuno-gold-silver test (IGST) for detection of antibodies to HIV.: Int Conf AIDS. 1991 Jun 16-21;7(1):111 (abstract no. M.A.1079). Unique Identifier : AIDSLINE ICA7/1107991; Mekushinov K; Vassilev V; Beshkov D; Laboratory on AIDS, Military Medical Academy, Sofia, Bulgaria; OBJECTIVE: To develop a high-sensitive and cheap test for screening of antibodies to HIV-1. METHODS: We used a nitrocellulose membrane, adsorbed with HIV-1 lysate (DuPont), ten-fold diluted sera for testing and own protein A-gold conjugate. Our new step is silver enhancing with silver nitrate and chlorides of iron as e
PCR detection of integrated HIV-1 DNA in peripheral blood lymphocytes (PBL) from infected individuals at various stages of disease using a non-isotopic microtiter plate assay.: Int Conf AIDS. 1991 Jun 16-21;7(1):112 (abstract no. M.A.1080). Unique Identifier : AIDSLINE ICA7/1108091; Dragon E; Kung K; Wolfe L; Casareale D; Giron J; PCR Division, Roche Diagnostic Systems Inc, Fair Lawn, NJ USA; OBJECTIVE: To evaluate a non-isotopic method for the detection for PCR amplified HIV-1 products using DNA isolated from 50 HIV-1 seropositive individuals (at various stages of infection). In addition, different methods of PBL isolation and processing were evaluated. METHODS: PBL, isolated from blood using Ficoll-Hypaqe
Evaluation of a new automated test (VIDAS HIV 1 + 2) for the detection of HIV antibodies.: Int Conf AIDS. 1991 Jun 16-21;7(1):112 (abstract no. M.A.1081). Unique Identifier : AIDSLINE ICA7/1108191; Suiphon E; Vernet G; Dalbon P; Barin F; bioMerieux S.A., Marcy l'Etoile, France; OBJECTIVE: To evaluate a new HIV 1 + 2 screening test developed on the automated immunoenzymatic system VIDAS on a panel of HIV-1 and HIV-2 positive sera. METHODS: Plastic tips, used as the solid phase, are coated with 2 synthetic peptides corresponding to transmembranous proteins from HIV-1 and HIV-2. Sera or plasma e
Quantitation of infectious virus from unfractionated whole blood (WB) in HIV-1 infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):112 (abstract no. M.A.1082). Unique Identifier : AIDSLINE ICA7/1108291; Ariyoshi K; Weber J; St Mary's Hospital Medical School, London, UK; OBJECTIVE: To compare the practicability and the implication of WB Tissue-Culture-Infectious Dose (TCID) in comparison with plasma TCID and PBMC TCID. METHODS: HIV-1 levels were quantified simultaneously in heparinized whole blood, plasma and PBMC by limiting dilution analysis in 15 HIV-1 infected individuals at diffee
Detection of HIV proviral DNA in the needles of intravenous drug users in the city of New Haven needle exchange program.: Int Conf AIDS. 1991 Jun 16-21;7(1):112 (abstract no. M.A.1083). Unique Identifier : AIDSLINE ICA7/1108391; Heimer R; Cadman EC; Kaplan EH; Dept. of Internal Medicine, New Haven, CT; OBJECTIVE: Employment of polymerase chain reaction (PCR) to amplify HIV proviral DNA flushed from syringes returned by IV drug users participating in New Haven s Needle Exchange Program. Determination of HIV prevalence and incidence among program participants can be calculated and compared to estimates. METHODS: DNA re
Reverse transcriptase activity measured by ELISA.: Int Conf AIDS. 1991 Jun 16-21;7(1):113 (abstract no. M.A.1084). Unique Identifier : AIDSLINE ICA7/1108491; Eberle J; Seibl R; Max V. Pettenkofer- Institute for Hygiene and Medical; Microbiology Ludwig- Maxmilians- University, Munich, Germany; OBJECTIVE: To develop an alternative assay for testing HIV reverse transcriptase (RT) activity in cell culture supernatants, avoiding the disadvantages of the standard procedure (radioactive reagents, difficult handling, expensive equipment). METHOD: In addition to deoxythymidine-triphosphate (dTTP), digoxigenin- and e
Earlier detection of anti-HIV-1 seroconversion by third generation anti-HIV-1/2 ELISA.: Int Conf AIDS. 1991 Jun 16-21;7(1):113 (abstract no. M.A.1085). Unique Identifier : AIDSLINE ICA7/1108591; Reesink HW; Plaisier AD; van der Poel CL; Pietersz RN; Lelie PN; Red Cross Blood Bank Amsterdam, Amsterdam, The Netherlands; OBJECTIVE: To establish the sensitivity of 2 new anti-HIV-1/2 ELISA s (Wellcome, Abbott) in a HIV-1 seroconversion panel. METHODS: Two anti-HIV-1/2 3rd generation ELISA s employ recombinant HIV-1 core, HIV-1 envelope and HIV-2 envelope protein as coating antigen and as conjugate. A panel of sequential serum samples ofe
HIV-1 expression in 25 infected patients: a comparison of RNA PCR, p24 EIA in plasma and in situ hybridization in mononuclear cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):113 (abstract no. M.A.1086). Unique Identifier : AIDSLINE ICA7/1108691; Delord B; Ottmann M; Schrive MH; Errecart MJ; Ragnaud JM; Seigneurin JM; Fleury HJ; CHRU de Bordeaux, Bordeaux, France; OBJECTIVE: To compare the data of in situ hybridization (ISH), RNA polymerase chain reaction (PCR) and p24 enzyme immuno-assay (EIA) for the detection of HIV-1 expression respectively in peripheral blood mononuclear cells (PBMC) and in plasma of infected patients at various CDC stages. METHODS: PBMC of 25 patients (moe
Non-isotopic PCR methods for the detection of HIV-1 in Ugandan mothers and infants.: Int Conf AIDS. 1991 Jun 16-21;7(1):113 (abstract no. M.A.1087). Unique Identifier : AIDSLINE ICA7/1108791; Jackson B; Ndugwa C; Mmiro F; Kataaha P; Guay L; Ball T; Dragon B; Goldfarb J; Xu S; Kabengera S; et al; Case Western Reserve U, Cleveland, OH, USA; OBJECTIVE: To determine the feasibility, sensitivity, and specificity of non-isotopic polymerase chain reaction (PCR) methods for the detection of HIV-1 in Ugandan mothers and infants. METHODS: PCR testing was performed on crude cell lysates of 42 Ugandan HIV-1 Western blot positive and 16 seronegative mothers and 55 e
HIV detection in blood monocytes from HIV seronegative subjects.: Int Conf AIDS. 1991 Jun 16-21;7(1):114 (abstract no. M.A.1088). Unique Identifier : AIDSLINE ICA7/1108891; Di Lorenzo A; Bergamini A; Perno CF; Rosci MA; Miceli M; Mannella E; Rocchi G; National Blood Transfusion Center of Italian Red Cross, Rome,; Italy; OBJECTIVE: To determine whether viral isolation from monocytes (M/M) of seronegative high risk subjects could be a valuable method for early detection of HIV infection. METHOD: Blood derived M/M from 18 (6 males and 12 females) heterosexual partners of seropositive subjects previously involved in long term unprotectede
Prognostic value of plasma HIV isolation in monocytes/macrophages cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):114 (abstract no. M.A.1089). Unique Identifier : AIDSLINE ICA7/1108991; Mannella E; Armignacco O; Mercurio G; Marinelli L; Di Lorenzo A; Bergamini A; Pezzella M; N.B.T.C. Italian Red Cross, Rome, Italy; OBJECTIVE: To assess the prognostic value of HIV isolation from normal monocytes/macrophages (M/M) exposed to plasma of ARC/AIDS subjects. METHODS: Blood derived M/M from a normal seronegative donor were obtained by five days adherence and then were cultured with plasma from 36 HIV positive patients (17 asymptomatic, e
HIV detected within alveolar macrophages in vivo by immunocytochemistry from induced sputum samples.: Int Conf AIDS. 1991 Jun 16-21;7(1):114 (abstract no. M.A.1090). Unique Identifier : AIDSLINE ICA7/1109091; Evans M; Watts S; Wansbrough-Jones M; St. George's Hospital Medical School, London, England; OBJECTIVE: To detect HIV within alveolar macrophages in vivo & to correlate with stage of disease. METHODS: Induced sputum samples were obtained from controls (no known risk factors for HIV), asymptomatic HIV+ve individuals & AIDS patients. After mucolytic treatment, alveolar cells were washed, fixed in acetone
NASBAtm nucleic acid amplification used for the detection of HIV-1 in clinical samples.: Int Conf AIDS. 1991 Jun 16-21;7(1):114 (abstract no. M.A.1091). Unique Identifier : AIDSLINE ICA7/1109191; Van Gemen B; van Strijp D; Schukkink R; Beyer R; Huisman H; Koppelman M; Malek L; Lens P; Organon Teknika, Boxtel, The Netherlands; The nucleic acid sequence based amplification (NASBAtm) technology was developed and tested using a HIV-1 model system and clinical samples. NASBAtm is an one step primer dependent isothermal amplification technique for both RNA and DNA templates. Amplification factors range from 10(6) to 10(9) depending on primer set
Application of PCR to discriminate between HIV-1 and HIV-2 infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):115 (abstract no. M.A.1092). Unique Identifier : AIDSLINE ICA7/1109291; Gorrino MT; Campelo C; Sarria L; Fernandez de Aranguiz A; Lardelli P; Suarez MD; Cisterna R; Microbiology and Immunology Department, University of Basque; Country, Bilbao, Spain; PCR has been proposed as a method to identify infected patients or blood donors before seroconversion is detected or to assess the results when the question of infection in an individual is ambiguous by serological assays. An additional potential application of the method is the discrimination between HIV-1 and HIV-2
Detection of HIV-1 DNA by PCR incorporation of digoxigenin-dutp and hybridization to immobilized probes.: Int Conf AIDS. 1991 Jun 16-21;7(1):115 (abstract no. M.A.1093). Unique Identifier : AIDSLINE ICA7/1109391; Costa Taveira N; Moniz Pereira J; Odette Ferreira M; University of Lisbon, Faculty of Pharmacy, Dept. of Microbiology,; Lisbon, Portugal; OBJECTIVE: To develop a simple, rapid and non-isotopic PCR-based method for the detection of HIV-1 DNA sequences present in the total DNA of PBMCs of HIV-1 infected individuals. METHODS: Polymerase chain reaction was performed as described by Laure, F. et. al. (The Lancet, Sept. 3, 1988, pg. 538-540), using a mixture
Detection of free and antibody-complexed antigen for the diagnosis of perinatal HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):115 (abstract no. M.A.1094). Unique Identifier : AIDSLINE ICA7/1109491; Palomba E; Gay V; Barberis E; Ciuti E; Perugini L; Nicola P; Tovo PA; Department of Pediatrics, University of Turin, Turin, Italy; OBJECTIVE: Acid treatment (pH 2.5-3) of serum, dissociating immune-complex, improves p24 antigen detection in HIV-infected adults. This test may prove useful for the early diagnosis of perinatal infection, when high titers of maternal antibody are still present in the infant s blood. METHODS: p24 antigen was assessed
International collaborative standardization of PCR assay for HIV1 DNA.: Int Conf AIDS. 1991 Jun 16-21;7(1):115 (abstract no. M.A.1095). Unique Identifier : AIDSLINE ICA7/1109591; Brechot C; Chaput A; Montagnier L; Osmanov S; Milman G; Institut Pasteur, Paris, France; OBJECTIVE: Thirty international laboratories participated in the PCR evaluation of a panel of samples containing various amounts of HIV-1 DNA. METHODS: DNA was prepared from the 8E5-LAV cell line containing one stabile HIV-1 DNA copy per cell. Specimens in the panel contained defined copy numbers of the 8E5-LAV DNA in
Construction and evaluation of a nested primer PCR for the detection of HIV-2 in clinical samples.: Int Conf AIDS. 1991 Jun 16-21;7(1):116 (abstract no. M.A.1096). Unique Identifier : AIDSLINE ICA7/1109691; Grankvist O; Bredberg-Raden U; Gustavsson A; Albert J; Albino P; Biberfeld G; Wadell G; Dept. of Virology, University of Umea, Sweden; OBJECTIVE: To design and evaluate a nested primer PCR for the detection of HIV-2 DNA in clinical samples. METHODS: A two-step-PCR with nested primer pairs from 4 regions was compared with a single-step-PCR with primer pairs from 3 regions, with or without hybridization on 17 HIV-2 isolates. 37 samples of blood mononuc
Prospective detection of HIV-1 proviral DNA in peripheral blood mononuclear cells of children born to seropositive mothers.: Int Conf AIDS. 1991 Jun 16-21;7(1):116 (abstract no. M.A.1097). Unique Identifier : AIDSLINE ICA7/1109791; Cirillo O; De Maria A; Ferrazin A; Loy A; Gotta C; Terragna A; Clinica Malattie Infettive, University of Genova, G. Gaslini; Institute, Italy; OBJECTIVE: To determine whether and how an early treatment decision could be based on information obtained by polymerase chain reaction (PCR) amplification of HIV-1 DNA in peripheral blood mononuclear cells (PBMC) from children born to HIV-1 seropositive mothers. METHODS: Children born to HIV-1 seropositive mothers we
Simple and efficient assay for Gp160 and Gp120 of various strains of HIV-1 and HIV-2.: Int Conf AIDS. 1991 Jun 16-21;7(1):116 (abstract no. M.A.1098). Unique Identifier : AIDSLINE ICA7/1109891; Mahmood N; Hay AJ; Hentschel C; MRC Collaborative Centre, Mill Hill, London MW7 1AD; Assay of the glycoproteins of HIV is useful in assessing HIV infection and in evaluating the antiviral activities of compounds in HIV infected cell cultures. Commercially available ELIZA kits have certain disadvantages in regard to cost and the restricted specificities of antibodies used. We have developed a novel ass
Identification of HIV-1 in paraffin wax embedded tissues.: Int Conf AIDS. 1991 Jun 16-21;7(1):116 (abstract no. M.A.1099). Unique Identifier : AIDSLINE ICA7/1109991; Pudney J; Anderson D; Harvard Medical School, Boston, MA, USA; OBJECTIVE: Investigate effects of different fixatives on the ability of commercially available monoclonal antibodies (Mab s) to detect HIV-1 in wax embedded tissues. METHODS: Jurkat cells infected with HIV-1b fixed in either unbuffered 10% formaldehyde (F), 2.5% gluteraldehyde (G), or a mixture of 4% paraformaldehyde
HIV-1 isolation from whole blood: standardization and applications.: Int Conf AIDS. 1991 Jun 16-21;7(1):117 (abstract no. M.A.1100). Unique Identifier : AIDSLINE ICA7/1110091; Fico C; Angarano G; Fiore JR; Di Stefano M; Monno L; Fracasso C; Pastore G., Clinic of Infectious Diseases, University of Bari,; Italy; OBJECTIVE: To evaluate HIV-1 isolation from small amounts of whole blood (W.B.) in order to develop a technique rapid, effective and easily achievable in the clinical practice. METHODS: We have studied 100 HIV-1 infected subjects (51 asymptomatics, 49 AIDS). HIV-1 isolation was attempted by using standard PMCs culture
In vitro lymphocyte activation enhances the detection of HIV-1 sequences by PCR in infected-seronegative individuals.: Int Conf AIDS. 1991 Jun 16-21;7(1):117 (abstract no. M.A.1101). Unique Identifier : AIDSLINE ICA7/1110191; Chen H; Carbonari M; Sbarigia D; Cherchi M; Pesce A; Aiuti F; Fiorilli M; University of Rome 'La Sapienza', Rome, Italy; OBJECTIVE: To determine whether: 1) in vitro activation of lymphocytes by polyclonal stimulation enhances the sensitivity of polymerase chain reaction (PCR) for the detection of HIV-1 sequences in infected-seronegative individuals; 2) in vitro production of HIV-1 specific antibodies occurs in infected-seronegatives. M
Detection, quantitation and sequencing of HIV-1 virus from the plasma of seropositive individuals and from factor VIII concentrates.: Int Conf AIDS. 1991 Jun 16-21;7(1):117 (abstract no. M.A.1102). Unique Identifier : AIDSLINE ICA7/1110291; Zhang LQ; Simmonds P; Ludlam CA; Leigh Brown AJ; Division of Biological Science, University of Edinburgh; Department of Haematology, Royal Infirmary, Edinburgh, UK; OBJECTIVE: To develop a sensitive RNA PCR method to detect, quantify and sequence HIV-1 viral RNA directly from the plasma or serum of seropositive individuals and from factor VIII concentrate. METHOD: HIV-1 viral RNA was extracted and DNAse treated prior to reverse transcription with HIV-specific anti-sense primers.
Early diagnosis of HIV infection in infancy: a multi-test evaluation.: Int Conf AIDS. 1991 Jun 16-21;7(1):117 (abstract no. M.A.1103). Unique Identifier : AIDSLINE ICA7/1110391; Pahwa S; Abrams E; Paul M; Tetali S; Wang X; Pasieka R; Napolitano B; North Shore University Hospital-Cornell University Medical; College, Manhasset, NY, USA; OBJECTIVE: To evaluate a battery of tests for diagnosis of HIV infection in infants (CDC class P-O), born to HIV seropositive women. METHODS: Methods included HIV culture of peripheral blood mononuclear cells (PBM) and of plasma; direct detection of serum p24 antigen by ELISA; polymerase chain reaction (PCR) with SK38
Virus isolation, polymerase chain reaction and in vitro antibody production for diagnosis of pediatric HIV1 infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):118 (abstract no. M.A.1104). Unique Identifier : AIDSLINE ICA7/1110491; Garbarg Chenon A; Vendrell JP; Silvain A; Courpotin C; Serre A; Nicolas JC; Grimprel E; Bricout F; CHU St Antione, Paris; The diagnosis of HIV1 infection in infants born to HIV seropositive mothers is critical. In this study we have compared the results obtained by virus isolation in cell culture (VC), gene amplification (PCR) and in vitro anti-HIV1 antibody production (IVAP) by peripheral blood mononuclear cells (PBMC). METHODS: 69 infa
A new plasmid construct as internal standard for HIV PCR.: Int Conf AIDS. 1991 Jun 16-21;7(1):118 (abstract no. M.A.1105). Unique Identifier : AIDSLINE ICA7/1110591; Telenti A; Imboden P; Institute for Medical Microbiology; University of Berne,; Switzerland; The use of internal standards in HIV PCR would fulfill two purposes: 1) Control of the efficiency of amplification for each individual sample, 2) facilitation of quantitative analysis of the results. We have constructed a new plasmid using gene splicing by overlap extension PCR techniques. This construct incorporates
Evaluation of four commercial HIV-1 antigen assays.: Int Conf AIDS. 1991 Jun 16-21;7(1):118 (abstract no. M.A.1106). Unique Identifier : AIDSLINE ICA7/1110691; Maniez M; Ferroni A; Dupressoir MV; Rouzioux C; Centre de Transfusion sanguine, Lille, France; OBJECTIVES: The evaluation of sensitivity, specificity and reproducibility of four HIV-1 antigens assays. MATERIAL AND METHODS: The four assays were produced by Abbott Laboratories (A), Coulter Electronics Inc. (C), Diagnostics Pasteur (DP) and Du Pont de Nemours Co. (DDN). Eight
Quantitative plasma viraemia: comparison of two techniques.: Int Conf AIDS. 1991 Jun 16-21;7(1):118 (abstract no. M.A.1107). Unique Identifier : AIDSLINE ICA7/1110791; Rouzioux C; Puel J; Agut H; Brun Vezinet F; Ferchal F; Fleury H; Tamalet C; Hopital Necker, Paris; OBJECTIVES: The evaluation of two techniques published in N.E.J.M. (Dec 14 1989) and having reported very different results in plasma viraemia quantitation. PATIENTS AND METHODS: Seven labs of the working group were involved in the study. Plasmas and cells from HIV-1 patients at different CD4 levels were selected. The
Quantitative detection of HIV in peripheral blood mononuclear cells from infected patients by polymerase chain reaction.: Int Conf AIDS. 1991 Jun 16-21;7(1):119 (abstract no. M.A.1108). Unique Identifier : AIDSLINE ICA7/1110891; Escaich S; Ritter J; Rougier P; Sepetjan M; Trepo C; INSERM U271; OBJECTIVE: To determine the number of proviral HIV-containing PBMC as an index of progression of HIV infection. METHODS: HIV proviral sequences were amplified by PCR using gag primers, and P32 labelled dCTP incorporation. For the sake of calibration, known copy numbers of HIV-containing pBT1 plasmid were amplified. Us
Detection of HIV plasma viremia by culture and polymerase chain reaction (PCR) at different stages of HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):119 (abstract no. M.A.1109). Unique Identifier : AIDSLINE ICA7/1110991; Escaich S; Ritter J; Rougier P; Lepot D; Lamelin JP; Sepetjan M; Trepo C; INSERM unite 271; OBJECTIVE: To study HIV replication in infected individuals by assessing cell-free virus production in blood. METHODS: Two hundred and twenty eight blood samples from 154 HIV seropositive subjects were investigated for the presence of HIV in plasma, using normal PBMC as the target for replication. PCR was used in para
Quality control of PCR assay for detection of HIV DNA in French laboratories.: Int Conf AIDS. 1991 Jun 16-21;7(1):119 (abstract no. M.A.1110). Unique Identifier : AIDSLINE ICA7/1111091; Anonymous; France; OBJECTIVES: 1) To determine the specificity, the sensitivity and the reproductibility of polymerase chain reaction (PCR) assay to detect HIV DNA in different French laboratories. 2) To reach a consensus on conditions of PCR assay in HIV infection. METHODS: Samples of frozen lymphocytes (8 to 10 x 10(6) cells per sampl
Comparison between HIV1 and HIV2 isolation from PBMC by a standardized coculture in 24-well plate.: Int Conf AIDS. 1991 Jun 16-21;7(1):119 (abstract no. M.A.1111). Unique Identifier : AIDSLINE ICA7/1111191; Pepin JM; Simon F; Bartczak S; Gamba E; Matheron S; Dazza MC; Brun Vezinet F; Laboratoire de Virologie, Hop. Bichat-Claude Bernard, Paris,; France; OBJECTIVE: To study the frequency of retrovirus isolation from HIV1 and HIV2 seropositive patients Peripheral Blood Mononuclear Cells (PBMC) in relation to clinical status. MATERIAL: Between June and December 1990, 76 consecutive PBMC were collected from HIV seropositive African and European patients diagnosed by WB e
Development of oligonucleotide primers and probes for amplification of African and North American strains of HIV-1 genome using polymerase chain reaction (PCR).: Int Conf AIDS. 1991 Jun 16-21;7(1):120 (abstract no. M.A.1112). Unique Identifier : AIDSLINE ICA7/1111291; Dawood M; Allan R; Fowke K; Hammond G; Cadham Provincial Laboratory, Winnipeg, Manitoba, Canada; OBJECTIVE: 1) To develop sensitive and specific oligonucleotide primers and probes for the amplification of HIV-1 genome using PCR; 2) To test the developed oligonucleotides on HIV-1 positive and negative African and North American blood samples; 3) To compare Southern blot (SB) & liquid hybridization (LH) for dete
Polymerase chain reaction (PCR) analysis on coculture supernatants for detecting HIV in body fluids.: Int Conf AIDS. 1991 Jun 16-21;7(1):120 (abstract no. M.A.1113). Unique Identifier : AIDSLINE ICA7/1111391; VedBrat SS; Pierce PF; Hellman KB; Braton Biotech Inc., Rockville, Md. USA; OBJECTIVE: We have examined the possibility of using PCR analysis of coculture supernatants (Sups) for early HIV detection to permit repeat testing of valuable clinical samples which either have extremely small numbers of cells or run the risk of contamination when analyzed in long term cocultures for HIV p24 antigen
Isolation of HIV from children in zidovudine therapy.: Int Conf AIDS. 1991 Jun 16-21;7(1):120 (abstract no. M.A.1114). Unique Identifier : AIDSLINE ICA7/1111491; Bernal A; Garcia Saiz A; Martin Fontelos P; Ortiz M; Perez Jurado ML; Pablos A; Najera R; CNBCR, MADRID, SPAIN; OBJECTIVE: To correlate biologic characterization of HIV strains isolated from symptomatic children in prolonged zidovudine therapy with susceptibility to antiviral drugs. METHODS: Isolation of HIV was attempted from heparinized blood, plasma and cerebrospinal fluid from fourteen children. Kinetics of replication were
Human immunodeficiency virus type 2 (HIV-2) strains: biologic heterogeneity.: Int Conf AIDS. 1991 Jun 16-21;7(1):120 (abstract no. M.A.1115). Unique Identifier : AIDSLINE ICA7/1111591; Buffet Janvresse C; Bertin C; Callens C; Coron D; Pons JL; De Matteis M; Ch.Nicolle Hospital and University, Rouen, France; OBJECTIVE: Biological characterization of HIV-2 strains isolated from peripheral blood mononuclear cells (PBMC) of Senegalese patients from France (Haute-Normandie region). METHODS: HIV-2 isolations were recovered from patients PBMC and cocultivated with an equal number of PHA stim
Plasma viremia in a randomly selected group of HIV seropositive individuals.: Int Conf AIDS. 1991 Jun 16-21;7(1):121 (abstract no. M.A.1116). Unique Identifier : AIDSLINE ICA7/1111691; Dewar RL; Sarmiento M; Lawton E; Clark H; Kennedy P; Shah A; Metcalf J; Lane HC; Salzman NP; Georgetown University, Washington, DC; OBJECTIVE: To analyze various parameters that affect the successful isolation of HIV from the plasma of seropositive individuals. METHODS: Whole blood samples collected in different anticoagulants were processed at various times after phlebotomy to obtain the plasma. Plasma was then used either fresh, or after a cycle
Monitoring IgG, IgA, and IgM responses to HIV infections by modified Abbott Matrix(TM) HIV-1/HIV-2 assay.: Int Conf AIDS. 1991 Jun 16-21;7(1):121 (abstract no. M.A.1117). Unique Identifier : AIDSLINE ICA7/1111791; Chan E; Schulze W; Leuther M; Knigge K; Abbott Laboratories, Abbott Park, Illinois, USA; OBJECTIVE: To develop tests for HIV-specific IgG, IgA, and IgM for the monitoring of individual immunological responses to HIV infection. METHODS: Biotinylated goat antibodies specific for IgG (gamma), IgA (alpha), and IgM (mu) were used in a semiautomated immunodot blot assay - The Abbott MATRIX HIV-1/HIV-2 assay. RE
Diagnosis of perinatal HIV-1 infection by means of double polymerase chain reaction (PCR).: Int Conf AIDS. 1991 Jun 16-21;7(1):121 (abstract no. M.A.1118). Unique Identifier : AIDSLINE ICA7/1111891; Simonon A; Vaira D; LePage P; Hitimana DG; Sondag Thull D; Van de Perre P; AIDS Reference Laboratory, National AIDS Control Program, Kigali,; Rwanda; OBJECTIVE: To determine the usefulness of double PCR for the diagnosis of perinatal HIV-1 infection. METHODS: Two cohorts of infants born to HIV-1 infected (n=218) and to uninfected mothers (n=216) have been prospectively followed up for 2 years in Kigali, Rwanda . HIV-1 Western blo
A comparative study of three different nucleic acid amplification assays for the detection of HIV-1 in infected individuals with antiviral therapy.: Int Conf AIDS. 1991 Jun 16-21;7(1):121 (abstract no. M.A.1119). Unique Identifier : AIDSLINE ICA7/1111991; Huisman H; Bruisten S; Koppelman M; Van Gemen B; Lens P; Klaassens R; Weigel H; Central Laboratory of the Bloodtransfusion Service, Amsterdam, NL; OBJECTIVE: We evaluate the correlation of the detection of HIV by different nucleic acid amplification procedures with the detection of HIV-antibody, HIV-antigen and clinical parameters. Of 31 persons at different stages of HIV infection, simultaneously whole blood, cell-free plasma, peripheral mononuclear cells (MNC)
Detection of specific HIV1 antibodies by the use of cyanogen bromide (CNBr)-activated paper discs.: Int Conf AIDS. 1991 Jun 16-21;7(1):122 (abstract no. M.A.1120). Unique Identifier : AIDSLINE ICA7/1112091; Ansotegui IJ; Lombardi VR; Scarlatti G; Plebani A; Boscherini B; Rossi P; Wigzell H; Department of Immunology, Karolinska Institute, Stockholm, Sweden; OBJECTIVE: To establish a new method for detecting specific antibodies against HIV1-peptides. METHODS: Synthetic peptides from different regions of the gp120 molecule were coupled to cyanogen bromide (CNBr)-activated paper discs. Based on the results previously obtained by HIV1-peptide ELISA, 100 positive and 50 negat
Detection of HIV-1 specific DNA in serum samples of infected people by polymerase chain reaction.: Int Conf AIDS. 1991 Jun 16-21;7(1):122 (abstract no. M.A.1121). Unique Identifier : AIDSLINE ICA7/1112191; Ovcak S; Likar M; Drinovec B; Institute of Microbiology, Ljubljana, Yugoslavia; OBJECTIVE: To detect whether viral DNA could be detected in the serum samples of infected people by use an in vitro DNA amplification procedure termed the polymerase chain reaction (PCR). METHODS: 22 sera of anti-HIV-1 positive asymptomatic individuals and 12 sera of patients with ARC or AIDS were evaluated. DNA was i
Human immunodeficiency virus type 1 viremia and HIV-1 transcripts detected by RT-PCR in clinical samples from infected patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):122 (abstract no. M.A.1122). Unique Identifier : AIDSLINE ICA7/1112291; Bagnarelli P; Clementi M; Menzo S; Manzin A; Giacca M; Varaldo PE; Institute of Microbiology, University of Ancona; OBJECTIVE: To define the diagnostic value of reverse-transcription polymerase chain reaction (RT-PCR) for the direct detection of (i) HIV-1 genomic RNA in plasma samples (HIV-1 viremia) and (ii) cellular specific viral transcripts in PBLs from HIV-1 infected subjects. METHODS: Genomic HIV-1 RNA from plasmas and HIV-1
The presence of budding virus-like particles in human lymphoid cells used for HIV cultivation.: Int Conf AIDS. 1991 Jun 16-21;7(1):122 (abstract no. M.A.1123). Unique Identifier : AIDSLINE ICA7/1112391; Dourmashkin RR; O'Toole CM; Bucher D; Oxford JS; The London Hospital Medical College, London, England; OBJECTIVES: To characterise cell tropism, morphology and infectivity of virus-like particles (VLP) in HIV infected and uninfected lymphoid cells. METHODS: Lymphoid cell lines were grown in RPMI 1640 with 10% FCS (or serum-free medium). Cord cells were isolated by Ficoll gradient and grown in the same medium. HIV IIIb
New assay for detection of replicating HIV by bioluminescence.: Int Conf AIDS. 1991 Jun 16-21;7(1):123 (abstract no. M.A.1124). Unique Identifier : AIDSLINE ICA7/1112491; Honigman A; Israel S; Lawatti P; Vonsover A; Ulitzur S; Swisa M; Bentwich Z; Dept. of Molecular Genetics, Hebrew University Medical School,; Jerusalem; OBJECTIVE: Develop an assay for active replicating HIV by transactivation of luciferase genes through HIV-TAT transacting polypeptide. METHODS: The Firefly luciferase gene was fused to the HIV-1 LTR and introduced into human kidney cell 293, using the pZIPNEOSV(x) retrovirus vector. The basal expression of the HIV pro
Detection and differentiation of HIV isolates by PCR analysis of the viral protease gene.: Int Conf AIDS. 1991 Jun 16-21;7(1):123 (abstract no. M.A.1125). Unique Identifier : AIDSLINE ICA7/1112591; Pieniazek D; Owen MS; Peralta JM; Krebs JW; Ferreira RF; Weniger BG; Pieniazek NJ; Schochetman G; Rayfield MA; Centers for Disease Control, Atlanta, GA, USA; OBJECTIVE: The viral protease gene contains both conserved and variable regions of sequence that display significant differences between HIV-1 and HIV-2. The conserved elements of the gene are useful in diagnostic detection of proviral DNA and genotyping of HIV-1 and -2 infections, whereas analysis of the variable seq
Unambiguous detection of HIV-1 proviral DNA in newborns from infected mothers by polymerase chain reaction.: Int Conf AIDS. 1991 Jun 16-21;7(1):123 (abstract no. M.A.1126). Unique Identifier : AIDSLINE ICA7/1112691; Salvi S; Tresoldi E; Gaudi S; Montermini L; Russo S; Siccardi AG; Novati R; Lazzarin A; Andronico F; Dipartim. Biol. Genet. Sci. Med., Milano, Italy; OBJECTIVE: We carried out the Polymerase Chain Reaction (PCR) on peripheral blood mononuclear cells DNA of newborns from infected mothers for an early detection of the provirus. The main goals of the work were: 1) to optimize the PCR conditions in order to obtain unambiguous results; 2) to assess the diagnostic value
Co-amplification of specific sequences of HIV-1 and HCV genomes by the polymerase chain reaction assay: a potential tool for the simultaneous detection of HIV-1 and HCV.: Int Conf AIDS. 1991 Jun 16-21;7(1):123 (abstract no. M.A.1127). Unique Identifier : AIDSLINE ICA7/1112791; Nedjar S; Hewlett I; Biswas R; Lab. of Hepatitis and Lab. of Retrovirology, DTS, CBER, FDA,; Bethesda, Maryland 20892, USA; OBJECTIVE: A rapid and simple method using the polymerase chain reaction (PCR), was devised for the co-amplification and simultaneous detection of human immunodeficiency virus, type 1, (HIV-1) and hepatitis C virus (HCV) specific sequences in the same clinical sample. METHODS: Genomic RNA was extracted from 13 blood d
PCR and virus isolation of HIV infected individuals: analysis of asymptomatic seropositive adults and mother/newborn pairs.: Int Conf AIDS. 1991 Jun 16-21;7(1):124 (abstract no. M.A.1128). Unique Identifier : AIDSLINE ICA7/1112891; Roques P; Vaslin B; Marce D; Dormont D; CEA and CRSSA, DSV/DPTE/SSA, Fontenay aux Roses, France; OBJECTIVE: To compare the efficacy of 3 different methods: polymerase chain reaction (PCR), peripheral blood mononuclear cells (PBMC) culture, and coculture in the study of the virological status of asymptomatic HIV infected individuals. METHODS: Patient Population: The cohort included 89 adults containing 16 well doc
Serum HIV antigen and anti-P24 antibodies levels in HIV seropositive patients: clinical evaluation of a new generation's semi-quantitative microelisa systems.: Int Conf AIDS. 1991 Jun 16-21;7(1):124 (abstract no. M.A.1129). Unique Identifier : AIDSLINE ICA7/1112991; Vignon D; Transfusion Center, Foch Hospital, France; Because serum Ag levels do correlate with the level of infectious virus in serum, Ag assays have been indicated for use of monitoring patients clinical disease progression and response to antiviral therapy. Moreover, progression to AIDS has been associated with a decline in anti-P24 levels, whereas antibodies to enve
Development of an augmented Western blot assay for characterization of samples reactive by combined HIV-1/HIV-2 screening assays.: Int Conf AIDS. 1991 Jun 16-21;7(1):124 (abstract no. M.A.1130). Unique Identifier : AIDSLINE ICA7/1113091; Chan L; Yin MF; Tay HS; Sum YW; Griffiths PJ; Reed D; Diagnostic Biotechnology, Singapore; OBJECTIVE: To develop a supplemental serological assay to characterize samples found reactive by combined HIV-1/HIV-2 screening assays. METHODS: An HIV-2 specific envelope peptide and anti-human IgG were slot blotted onto one end of nitrocellulose Western blotted with HIV-1 viral lysate. The sensitivity and specificit
Enzimatic amplification of HIV-1 DNA in peripheral blood mononuclear cells from pediatric patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):124 (abstract no. M.A.1131). Unique Identifier : AIDSLINE ICA7/1113191; Alba C; Delgado R; Fuertes A; Ruiz CJ; Gomez CE; Noriega AR; Lopez AD; Hospital 12 de Octubre, Madrid, Spain; OBJECTIVE: The purpose of this work was to compare the diagnostic value of PCR and p24 antigen in blood from children born to HIV seropositive mothers. METHODS: Patients were classified using CDC criteria for pediatric HIV infection. The group was very heterogeneous in relation to age at the time of the blood sampling
Matrix HIV-I/HIV-II: a new alternative to the Western blot.: Int Conf AIDS. 1991 Jun 16-21;7(1):125 (abstract no. M.A.1132). Unique Identifier : AIDSLINE ICA7/1113291; Niedbalski JS; Dahlen S; Braun B; Knigge K; Stephens J; Daluga C; Whiteside M; Abbott Laboratories, Abbott Park, IL 60064; A new semi-automated HIV assay (Matrix HIV-I/HIV-II) has been introduced as a Research Use Only product. This new assay system shows sensitivity and specificity to antibodies simultaneously against both HIV-I and HIV-II. Highly purified recombinant proteins (greater than 90%) of HIV-I and HIV-II are immobilized on a s
HIV isolation from plasma, lymphocytes and whole blood in subjects in different stages of infection and chemotherapeutic treatment.: Int Conf AIDS. 1991 Jun 16-21;7(1):125 (abstract no. M.A.1133). Unique Identifier : AIDSLINE ICA7/1113391; Sigari G; Losi E; Zernignan M; Arenare L; Brignole G; Wicks R; Cuneo Crovari P; Institute of Hygiene and Preventive Medicine, Genoa, University,; Genoa, Italy; OBJECTIVE: The study was carried out in order to evaluate whether HIV detection in plasma can provide more precise information on the infection development in comparison with virus detection in lymphocytes (PBMC), with or without Zidovudine treatment. We tried moreover to verify the efficiency and the significance of
Development and evaluation of biosensors for HIV-serology.: Int Conf AIDS. 1991 Jun 16-21;7(1):125 (abstract no. M.A.1134). Unique Identifier : AIDSLINE ICA7/1113491; Aberl F; Woias P; Koch S; KoBlinger C; Drost S; Wolf H; Institut fur Molekulare und Tumorvirologie am Max von; Pettenkofer-Institut, Ludwig-Maximiliains-Universitat Munchen; During the last decade a new generation of sensors, the biosensors, has been developed for the quantitative measurement of biological substances. Application and specifity of a biosensor is determined by the recognizing biological/biochemical layer and the type of transducer. According to the transducer used, biosenso
Diagnosis of vertical HIV-1 transmission using PCR and dried blood spot (dbs) specimens.: Int Conf AIDS. 1991 Jun 16-21;7(1):125 (abstract no. M.A.1135). Unique Identifier : AIDSLINE ICA7/1113591; Cassol S; Lapointe N; Salas T; Hankins O; Arella M; Fauvel M; O'Shaughnessy MV; Federal Centre for AIDS, Health and Welfare; OBJECTIVE: To conduct a blinded, prospective study using PCR to identify HIV DNA in dbs samples collected from infants perinatally exposed to HIV. METHODS: A total of 193 serial dbs samples from a cohort of 26 mothers and 44 children were tested for HIV-1 specific sequences using primers to env and gag. To determine t
Serological and molecular analysis of HIV-1 and HIV-2 double profiles.: Int Conf AIDS. 1991 Jun 16-21;7(1):126 (abstract no. M.A.1136). Unique Identifier : AIDSLINE ICA7/1113691; Leonard G; Courgnaud V; Sangare A; Denis F; Brechot C; Virology department, CHU Limoges, France; OBJECTIVE: To analyse serological HIV-1 and HIV-2 double profiles in different groups of population of Ivory Coast by mean of PCR. METHODS: Individuals from groups representative of the general population and patients with AIDS or AIDS-like diseases were recruited in 1990-1991
Contamination and its avoidance in laboratories performing HIV-1 PCR.: Int Conf AIDS. 1991 Jun 16-21;7(1):126 (abstract no. M.A.1137). Unique Identifier : AIDSLINE ICA7/1113791; Dwyor DE; Saksena NK; Unite d'Oncologie Virale and Unite de Biologie de Retrovirus,; Institut Pasteur, Paris, France; More laboratories are starting to use the polymerase chain reaction as a diagnostic tool in HIV-1 infection. The major complication of PCR is the detection of false positive reactions, usually due to previously amplified PCR products. To investigate this problem in two laboratories that undertake HIV cultures, PCR and
Polymerase chain reaction (PCR) sensitivity and specificity in infants and children.: Int Conf AIDS. 1991 Jun 16-21;7(1):126 (abstract no. M.A.1138). Unique Identifier : AIDSLINE ICA7/1113891; Nelson R; Price L; Di Nicolo R; Halsey A; Day N; Lockey R; Good R; University of South Florida, Department of Pediatrics and All; Children's Hospital, St. Petersburg/Tampa, FL, USA; OBJECTIVE: To assess the sensitivity and specificity of PCR for establishing the diagnosis of HIV infection in infants and children. METHODS: Serial testing was performed on samples from seropositive and seronegative infants at risk for HIV infection and from seropositive children. PCR was done using the primer pair S
HIV isolation and antigenemia in hemophiliacs and in homosexual men.: Int Conf AIDS. 1991 Jun 16-21;7(1):126 (abstract no. M.A.1139). Unique Identifier : AIDSLINE ICA7/1113991; van den Akker R; Vijge E; Meijer A; Mauser E; Borleffs J; van Loon A; RIVM, Lab. of Virology, Bilthoven, The Netherlands; OBJECTIVE: To follow virological events in HIV seropositive hemophiliacs and homosexual men. METHODS: 10x10(6) ficollgradient purified peripheral blood mononuclear cells (PBMC) were incubated with three days-PHA stimulated PBMC from healthy donors. Twice weekly the culture supernatants were examined for viral p24 prot
Chemically induced mutants of the CEM cell line demonstrate partial blockade of their PKC-dependent pathways and reduced susceptibility to HIV-1 infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):127 (abstract no. M.A.1140). Unique Identifier : AIDSLINE ICA7/1114091; Hillman K; Gruber MF; Kaufman J; Blackburn R; Manischewitz J; Norcross M; Golding H; Division of Virology, CBER, FDA, Bethesda, Maryland, USA; The mutagen EMS was used to derive mutants from the human cell line CEM. Several mutants, expressing significant number of CD4 receptors, and binding gp120, showed reduced susceptibility to infection with multiple strains of HIV-1 as judged by syncytia formation and RT assays. Quantitative PCR assays following infecti
Fine analysis of the immunodominant region from HIV transmembrane protein by chemical modification of the synthetic peptides.: Int Conf AIDS. 1991 Jun 16-21;7(1):127 (abstract no. M.A.1141). Unique Identifier : AIDSLINE ICA7/1114191; Andreev SM; Mescherjakova DV; Azmuko AA; Khaitov RM; Institute of Immunology, USSR Ministry of Public Health, Moscow; OBJECTIVE: To define the amino acids involved in IgG reactivity to five HIV-1 gp41 peptides overlapping the sequence 584-624, the method based on the chemical modification of three-functional amino acids, especially Lys residues, was suggested. METHODS: The acetylation of LysE-amino group(pH 8-9) was used. The reactiv
Immunoreactivity of selected peptides from HIV-1 gp120 principle neutralizing domain (PND) and CD4-binding region (CD4-BR).: Int Conf AIDS. 1991 Jun 16-21;7(1):127 (abstract no. M.A.1142). Unique Identifier : AIDSLINE ICA7/1114291; Khaitov RM; Mescherjakova DV; Andreev SM; Sidorova MV; Trubcheninova L; Vafina MG; Institute of Immunology, USSR Ministry of Public Health, Moscow; OBJECTIVE: To investigate the serum reactivity of HIV-1 infected individuals with the set of the overlapping synthetic peptides derived from PND of the BRU and MN strains (3 peptides) and CD4-BR of the BRU (4 peptides); to evaluate the correlation between the presence of antibody response to PND, CD4-BR and the clinic
Interaction of HIV-1 fusion peptides with lipid membranes.: Int Conf AIDS. 1991 Jun 16-21;7(1):127 (abstract no. M.A.1143). Unique Identifier : AIDSLINE ICA7/1114391; Slepushkin VA; Sidorova MV; Andreev SM; Melikyan GB; Chumakov VM; Manukyan RA; Grigoriev VB; Karamov EV; D.I. Ivanovsky Institute of Virology, Moscow; OBJECTIVE: To investigate the influence of the structure of fusion peptides on their interaction with artificial lipid membranes. METHODS and RESULTS: Two families of peptides were synthesized. The C-terminus of the peptides from the first family was located at the glycine-532 of gp160 HIV(BRU str.) and they were asse
Allosteric properties of heterodimeric HIV-1 reverse transcriptase.: Int Conf AIDS. 1991 Jun 16-21;7(1):128 (abstract no. M.A.1144). Unique Identifier : AIDSLINE ICA7/1114491; Debyser Z; Pauwels R; Andries K; Desmyter J; Engelborghs Y; Janssen PA; De Clercq E; Rega Institute for Medical Research, K.U.Leuven, Leuven, Belgium; OBJECTIVE: The reverse transcriptase (RT) of HIV-1 is present in virions and infected cells as an heterodimer (p66/p51). A new class of potent and selective HIV-1 inhibitors, the TIBO derivatives, was found to exert its antiviral activity by interacting in a unique fashion with monomeric HIV-1 RT (p66). We have now ex
Investigations into the content of nucleic acids trapped within antigen-substructures of HIV-1 by the polymerase chain reaction (PCR).: Int Conf AIDS. 1991 Jun 16-21;7(1):128 (abstract no. M.A.1145). Unique Identifier : AIDSLINE ICA7/1114591; Dennin RH; Beyer A; Institut fur Medizinische Mikrobiologie, Medizinische; Universitat zu Lubeck, Lubeck, Federal Republic of Germany; OBJECTIVE: This study was projected to characterize the structures which immunologically behave an antigens (AG) of HIV-1 not denatured by detergents in the cell free supernatant of HIV-1-infected cell cultures as well as in serum/plasma from HIV-1-infected individuals. The special question raised here was to look fi
Mutagen-mediated activation of HIV-1 expression.: Int Conf AIDS. 1991 Jun 16-21;7(1):128 (abstract no. M.A.1146). Unique Identifier : AIDSLINE ICA7/1114691; Quinto I; Mallardo M; Ruocco MR; Squitieri B; Scala G; Dipartimento di Biochimica e Biotecnologie Mediche, 11 Facolta di; Medicina e Chirurgia Universita degli Studi di Napoli, Naples,; Italy; OBJECTIVE: The aim of the present study was to analyse the molecular mechanisms involved in the activation of HIV-1 expression by mutagens in infected cells. We addressed the following questions: 1) Is the nature of DNA lesion relevant for triggering the activation of the viral RNA transcription? 2) Which cis-acting ci
Cytogenetic analysis of human cells infected with human immunodeficiency virus in vitro.: Int Conf AIDS. 1991 Jun 16-21;7(1):128 (abstract no. M.A.1147). Unique Identifier : AIDSLINE ICA7/1114791; Kushch A; Gluchova LA; Shumay EP; Mamaeva SE; Nossik DN; Kalnina LB; Grabovskaya IW; Tugizov SM; Asjo B; The D.I. Ivanovsky Institute of Virology, Moscow, USSR; OBJECTIVE: We studied the effect of HIV infection on the karyotype of two highly sensitive to HIV lymphoblastoid T cell lines. METHODS: The cell lines Jurkat-tat and MT-4 have been infected with BRU strain of HIV-1. Metaphase chromosomes were subjected to G-, C-banding and Ag-stained. HIV specific proteins was monitori
Virus population sequence analysis of a successful SIV vaccine.: Int Conf AIDS. 1991 Jun 16-21;7(1):129 (abstract no. M.A.1148). Unique Identifier : AIDSLINE ICA7/1114891; Slade A; Almond N; Szotyori Z; Jenkins A; Jones S; Kitchin P; AIDS Collaborating Centre, National Institute for Biological; Standards and Control, Potters Bar, Herts, UK; OBJECTIVE: To characterise the sequence repertoire of the SIVmac251 (32H) isolate. This isolate was used to demonstrate protection of cynomolgus macaques against SIV by a fixed infected-cell vaccine (Stott EJ et al, 1990, Lancet 336; 1538-41). METHODS: The polymerase chain reaction was used to amplify SIV genes from ti
HIV-1 regulatory and accessory gene mRNAs.: Int Conf AIDS. 1991 Jun 16-21;7(1):129 (abstract no. M.A.1149). Unique Identifier : AIDSLINE ICA7/1114991; Deacon N; Smith JM; Macfarlane Burnet Centre for Medical Research, Melbourne,; Victoria, Australia; OBJECTIVE: As well as the three structural polyproteins, HIV-1 expresses at least 8 regulatory and accessory proteins encoded by singly and multiply spliced mRNAs. We have set out to characterise HIV-1 spliced mRNAs, the splice donor and acceptor sites used and the temporal expression of the different species. METHODSi
Cloning of conserved and unique sequences of the HIV-1 genome for use as nucleic acid probes.: Int Conf AIDS. 1991 Jun 16-21;7(1):129 (abstract no. M.A.1150). Unique Identifier : AIDSLINE ICA7/1115091; Materazzo M; McQueen PW; Delaney SF; Department of Biotechnology, University of New South Wales,; Kensington, NSW, Australia; To develop a hybridisation test to detect the HIV-1 genome and to distinguish between active and latent forms of the virus, conserved and unique sequences of the gag and pol regions have been cloned into the transcription vector, pGEM-3Z. pARV/7A, a recombinant plasmid containing the HIV-1 DNA genome cloned into pUC19i
Regulation of HIV gene expression by Class 1 HLA antigens: transcription of kB-driven genes is induced by triggering Class 1 molecules on T-cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):130 (abstract no. M.A.1152). Unique Identifier : AIDSLINE ICA7/1115291; Turco MC; Lamberti A; Bisogni R; De Lorenzo F; Petrella A; Venuta S; Dipartimento di Biochimica e Biotecnologie Mediche, II Facolta; di Medicina, Napoli, Italy; OBJECTIVE: Our research goal is to demonstrate that the triggering of Class I HLA molecules on T-cell surface regulates cellular expression of genes controlled by kB elements at their 5 . These include HIV genes. METHODS: Human primary T lymphocytes were transfected by electroporation with plasmid constructs containini
Characterization of large glycosylated gag-encoded proteins expressed on the surface of HIV-infected cells which react with monoclonal antibodies against the HIV matrix protein, p17gag.: Int Conf AIDS. 1991 Jun 16-21;7(1):130 (abstract no. M.A.1153). Unique Identifier : AIDSLINE ICA7/1115391; Pinter A; Honnen WJ; Shang F; Revesz K; Herz R; Laboratory of Retroviral Biology, Public Health Research; Institute, 455 First Avenue, New York, New York 10016; OBJECTIVE: To molecularly characterize cell surface molecules identified by monoclonal antibodies against the matrix protein of HIV-1, p17gag. METHODS: Monoclonal antibodies against the matrix protein of HIV-1, p17gag, were isolated from rats which had been immunized with solubilized HIV-1 lysate. The affinity-purifiei
Expression and purification of soluble HIV-1 tat protein from E-coli.: Int Conf AIDS. 1991 Jun 16-21;7(1):130 (abstract no. M.A.1154). Unique Identifier : AIDSLINE ICA7/1115491; D'Souza ED; Cousens DJ; The Wellcome Foundation Ltd., Dept. of Molecular Sciences, South; Eden Park Road, Beckenham, Kent, England; OBJECTIVE: Structural studies on HIV Tat have in large been impaired by the inability to obtain sufficient amounts of native purified protein. All procedures reported to date for the purification of HIV Tat have relied on the use of strong denaturants and subsequent refolding of the Tat molecule. We report here the fii
Mechanism of action of the HIV-1 tat protein: interaction between tat, cellular factors and viral DNA and RNA targets.: Int Conf AIDS. 1991 Jun 16-21;7(1):130 (abstract no. M.A.1155). Unique Identifier : AIDSLINE ICA7/1115591; Jeang KT; Berkhout B; Gatignol A; Laboratory of Molecular Microbiology, NIAID, NIH, Bethesda, MD,; USA; OBJECTIVE: To study the mechanism of action of HIV-1 Tat. METHODS: Standard molecular techniques were used. RESULTS: We will show that upstream promoter elements and the HIV-1 TATAA sequence play an important role in Tat trans-activation. We will present evidence that Tat trans-activation occurs during a kinetically bi
Characterization of vif mutants of HIV-1 and HIV-2.: Int Conf AIDS. 1991 Jun 16-21;7(1):131 (abstract no. M.A.1156). Unique Identifier : AIDSLINE ICA7/1115691; Peden K; Fan L; Laboratory of Molecular Microbiology, NIAID, NIH, Bethesda, MD,; USA; OBJECTIVE: To determine the phenotype of mutants of HIV-1 and HIV-2 with defects in the vif gene in order to investigate the role of the Vif protein in the life cycle of these viruses. METHODS: Insertion and deletion mutations were introduced into the vif genes of infectious molecular clones of HIV-1 and HIV-2; in somi
Molecular cloning and complete sequence analysis of a highly divergent African HIV isolate.: Int Conf AIDS. 1991 Jun 16-21;7(1):131 (abstract no. M.A.1157). Unique Identifier : AIDSLINE ICA7/1115791; Vanden Haesevelde M; Decourt JL; Heyndrickx L; Vanderborght B; Saman E; DeLeys R; van der Groen G; Van Heuverswyn H; Innogenetics N.V. - Antwerpen - Belgium; OBJECTIVE: The molecular analysis of the proviral DNA from a divergent HIV isolate (Ant70). The sequence data obtained allow the detailed study of the differences with known HIV strains and the determination of the position of this new variant within the HIV family. METHODS: DNA from MT4 cells persistently infected wii
Human hepatoblastoma (HEPGZ) cells contain a TAR-binding nuclear factor that transactivates HIV-1 LTR expression.: Int Conf AIDS. 1991 Jun 16-21;7(1):131 (abstract no. M.A.1158). Unique Identifier : AIDSLINE ICA7/1115891; Pizzella T; Banerjee R; Giusti G; Acs G; Clinica Malattie Infettive, I Fac. Medicina, Universita di; Napoli, Italy; The expression of HIV-1 is regulated by both viral and cellular proteins as well as the trans-activator protein tat, which binds to a cis-acting element TAR, located in the long terminal repeats (LTR). Our group has recently reported that HepG2 cells can support transient HIV-1 LTR-directed CAT gene expression in the i
HIV-1 LTR promoter is functional in yeast Schizosaccharomyces pombe.: Int Conf AIDS. 1991 Jun 16-21;7(1):131 (abstract no. M.A.1159). Unique Identifier : AIDSLINE ICA7/1115991; Dhar R; Toyama R; Laboratory of Molecular Virology, National Cancer Institute,; National Institutes of Health, Bethesda, Maryland 20892; OBJECTIVE: To study HIV-LTR promoter activity and trans-activation by HIV-1 tat protein in yeast Schizosaccharomyces pombe. METHODS: Yeast replicating vectors containing HIV-1 LTR-CAT and different deletions of the HIV-LTR promoter were transfected into S. pombe cells. In separate experiments, SV40 promoter transcribii
Expression of homeogenes in HIV infected T-cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):132 (abstract no. M.A.1160). Unique Identifier : AIDSLINE ICA7/1116091; Frezza D; Santini SM; Migliacco E; Bach S; Boncinelli E; Colizzi V; Dipartimento di Biologia, II Universiti di Roma, Tor Vergata,; Rome, Italy; The aim of this project is to investigate the involvement of human homeogenes in T lymphocyte differentiation and whether HIV infection could modify their expression causing functional alterations. Homeotic genes were originally defined as genes controlling embryonic development in Drosophila. Homologous sequences weri
Binding of nuclear factors to the HIV-1 genome: biochemical characterization and inhibition by aromatic polyamidines.: Int Conf AIDS. 1991 Jun 16-21;7(1):132 (abstract no. M.A.1161). Unique Identifier : AIDSLINE ICA7/1116191; Feriotto G; Nastruzzi C; Volinia S; Giacomini P; Gambari R; Biochemistry Institute; DNA elements of HIV-1 putatively able to bind nuclear proteins have been identified by computer-assisted analyses using PC programs elsewhere described (1). By means of gel retardation and CAT activity assays we have identified a nuclear protein (X-B3) that specifically binds an HIV-1 tat-IVS region containing a DNA si
Cloning of an HIV-1 neutralizing V3 specific monoclonal antibody and expression as a mouse-human chimaeric antigen binding fragment and antibody.: Int Conf AIDS. 1991 Jun 16-21;7(1):132 (abstract no. M.A.1162). Unique Identifier : AIDSLINE ICA7/1116291; Marks JD; Wahren B; Gilljam G; Hinkula J; Winter G; MRC Aids Directed Programme, Laboratory of Molecular Biology,; Cambridge, UK; OBJECTIVE: To clone and sequence the variable domains of an HIV-1 neutralizing V3 specific murine monoclonal antibody (Mab) and use them to construct a mouse-human chimaeric antigen binding fragment (Fab) and mouse-human chimaeric IgG1 isotype antibody. METHODS: The most neutralization potent (Mab F58) of a battery ofi
Chromosomal integration sites of endogenous retroviral sequences.: Int Conf AIDS. 1991 Jun 16-21;7(1):132 (abstract no. M.A.1163). Unique Identifier : AIDSLINE ICA7/1116391; Taruscio D; Manuelidis L; Yale Medical School, New Haven, CT, USA; We analyzed the chromosomal integration sites of a human endogenous retrovirus (HERV). HERV is present in 50-100 copies in the genome and is related to MLV. Using high resolution in-situ hybridization, HERV integration sites were localized with respect to chromosomal bands containing high concentrations of Alu repeatsi
Antibodies to HIV p27 nef protein in 63 Zairian HIV seroconverters.: Int Conf AIDS. 1991 Jun 16-21;7(1):133 (abstract no. M.A.1164). Unique Identifier : AIDSLINE ICA7/1116491; Edidi B; Behets F; Kambembo L; Tshebuye M; Kayigamba K; Brown C; Quinn T; Projet SIDA, Kinshasa, Zaire; OBJECTIVE: To determine if antibodies to p27 nef can be detected before and at the time of seroconversion in a cohort of Zairian prostitutes. METHODS: A cohort of 575 initially HIV seronegative prostitutes was enrolled in a counseling and STD treatment program. Blood samples were obtained from each patient every threei
Deletion mapping of functional domains of HIV-1 gag polyprotein expressed in baculovirus-infected cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):133 (abstract no. M.A.1165). Unique Identifier : AIDSLINE ICA7/1116591; Boulanger P; Royer M; Gay B; Hong SS; Laboratoire de Virologie & Pathogeneese Moleculaires, Faculte; de Medecine, 34060 Montpellier, France; OBJECTIVE & METHODS: The functional domains of HIV-1 gag implicated in (i) its self-assembly, and (ii) its cellular localization, were determined using 8 recombinant baculoviruses (AcNPV) harboring various forms of full-length or truncated gag gene expressed under the control of the polyhedrin promoter. RESULTS: (i
Expression and activity of HIV-1 protease in recombinant baculovirus-infected cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):133 (abstract no. M.A.1166). Unique Identifier : AIDSLINE ICA7/1116691; Royer M; Boulanger P; Laboratoire de Virologie & Pathogenese Moleculaires, Faculte; de Medecine, 34060, Montpellier, France; OBJECTIVE: To determine the conditions and parameters for (i) high level expression, (ii) efficient self-processing, and (iii) proteolytic activity of recombinant HIV protease (PR) expressed in eucaryotic non-mammalian cells. METHODS: Six recombinant baculoviruses (AcNPV-PR) expressing various forms of polyhedrin (PH)
Biological function of HIV-1 nef.: Int Conf AIDS. 1991 Jun 16-21;7(1):133 (abstract no. M.A.1167). Unique Identifier : AIDSLINE ICA7/1116791; Tsunetsugu Yokota Y; Takebe Y; Saito T; Takemori T; Department of Cellular Immunology, AIDS Research Center, National; Institute of Health, Tokyo, Japan; OBJECTIVE: To study the biological function of the HIV-nef in HIV-infected cells. METHODS: 1) The vector containing HIV-nef under the constitutive SRalpha promoter was constructed (SRalpha-nef). SRalpha-nef carrying frame-shift termination was served as a control (SRalpha-nef). These vectors were transfected to Hela-C
Efficient production of a soluble HIV-1 reverse transcriptase in insect cells by a recombinant baculovirus - purification and characterization of the recombinant enzyme.: Int Conf AIDS. 1991 Jun 16-21;7(1):134 (abstract no. M.A.1168). Unique Identifier : AIDSLINE ICA7/1116891; Moosmayer D; Jung R; Petry H; Jentsch KD; Luke W; Hunsmann G; German Primate Center, Gottingen, Germany; OBJECTIVE: The reverse transcriptase (RT) of HIV is essential for virus replication and thus a potential target for antiviral chemotherapy. Large quantities of HIV RT are necessary to develop and evaluate RT inhibitors. METHODS: The large HIV-1 RT subunit encoding region was cloned into the transfer vector p36-C (the
Molecular modeling of gp120: comparison of predicted molecular topography with functional properties of the molecule.: Int Conf AIDS. 1991 Jun 16-21;7(1):134 (abstract no. M.A.1169). Unique Identifier : AIDSLINE ICA7/1116991; Mitchell WM; Gabriel JL; Dept. of Pathology, Vanderbilt University School of Medicine,; Nashville, TN 37232; OBJECTIVE: The molecular topography of gp120 is essential to understand the binding of HIV to the CD4 receptor, the association with gp41 and its fusogenic properties, and the role of N-glycosylation on conformation, infectivity, and antigenicity. Molecular modeling using a combination of energy minimization and molec
A dyad symmetry motif in the negative regulatory element of HIV-1 long terminal repeats downregulates transcription.: Int Conf AIDS. 1991 Jun 16-21;7(1):134 (abstract no. M.A.1170). Unique Identifier : AIDSLINE ICA7/1117091; Giacca M; Gutierrez MI; d'Adda di Fagagna F; Menzo S; Falaschi A; International Centre for Genetic Engineering and Biotechnology -; Trieste, Italy; OBJECTIVE: In the Negative Regulatory Element (NRE) of the Long Terminal Repeats (LTRs) of HIV-1, a dyad symmetry element CACGTG is located at position -166 upstream of transcription start site. This sequence is present as transcriptional element upstream of many eukaryotic and viral genes, including the Major Late Pr
Epitope mapping of the low-molecular-mass subunits of reverse transcriptase in HIV-1 by monoclonal antibodies.: Int Conf AIDS. 1991 Jun 16-21;7(1):134 (abstract no. M.A.1171). Unique Identifier : AIDSLINE ICA7/1117191; Chandra P; Gerber T; Chandra A; Sarin PS; Pavlikov S; Sidorovitch I; Khaitov R; Ivanov V; Koziach A; Laboratory of Molecular Biology (ZBC), Frankfurt University; Medical School (FRG); OBJECTIVE: We have shown that the biochemical heterogeneity of RT seen on iso-electric focusing is a unique feature of HIV-1 RT; RT purified from HIV-2, SIVagm, HTLV-1 and HTLV-II did not show this heterogeneity (FEBS-Lett. 197: 84-88, 1986). Our objective is to characterize the two species of HIV-1 RT separated on IE
Quantitation of HIV DNA by PCR in seropositive navy personnel.: Int Conf AIDS. 1991 Jun 16-21;7(1):135 (abstract no. M.A.1172). Unique Identifier : AIDSLINE ICA7/1117291; Ferre F; Lewis D; Marchese A; Wallace M; Beecham J; Duffy C; Gersten M; Burnett K; Jensen F; Carlo D; et al; Immune Response Corp.; OBJECTIVE: To develop an accurate quantitative HIV PCR assay to assess the efficacy of the IRC HIV-1 immunogen in clinical trials. METHODS: Quantitation was based on direct labelling of the PCR reaction and comparison with known amounts of HIV DNA (standard curve). One set of HIV primers was used (SK38/SK39) and one o
The EBNA2 gene of epstein barr virus (EBV) trans-activates the HIV long terminal repeat sequences (HIV-LTR).: Int Conf AIDS. 1991 Jun 16-21;7(1):135 (abstract no. M.A.1173). Unique Identifier : AIDSLINE ICA7/1117391; Scala G; Mallardo M; Ruocco MR; Squitieri B; Venuta S; Quinto I; II Medical School, University of Naples, Naples, Italy; OBJECTIVE: This study investigated the relationships between HIV and EBV, two viruses often clinically associated in AIDS patients. METHODS: We have transfected DeFew and EW36 cells, two EBV-negative B lymphomas, with the pZip-EBNA2 vector, which carries the EBNA2 gene together with the G418-resistance gene. Stably De
Sequence-specific recognition of CAR/RRE, the target sequence for the REV axis of HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):135 (abstract no. M.A.1174). Unique Identifier : AIDSLINE ICA7/1117491; Dayton ET; Konings D; Powell D; Shapiro B; Maizel J; Dayton AI; Laboratory of Immunoregulation, National Institute of Allergy &; Infectious Disease, NIH Bethesda, MD, USA; OBJECTIVE: To determine which bases in CAR/RRE are involved in sequence-specific recognition by the elements of the REV axis of HIV autoregulation. METHODS: We have devised a novel method of analysis called modified base-switching to design RNA target sequence analogues with secondary structures identical to wild-type
Defining the role of NEF in the transition from a quiescent HIV infection to an acute disease state.: Int Conf AIDS. 1991 Jun 16-21;7(1):135 (abstract no. M.A.1175). Unique Identifier : AIDSLINE ICA7/1117591; Psallidopoulos M; Newell M; Natarajan V; Lane HC; Salzman NP; Georgetown University, Washington DC, USA; OBJECTIVE: To determine the function of nef in the HIV-1 life cycle and to study changes in nef expression during the transition from a quiescent HIV infection to an acute disease state, associated with a pronounced decline in T4 cell counts. This activation from a latent viral infection to viral productivity is hypot
Structure-function analysis of glycan variants of HIV-1 envelope glycoproteins.: Int Conf AIDS. 1991 Jun 16-21;7(1):136 (abstract no. M.A.1176). Unique Identifier : AIDSLINE ICA7/1117691; Vasudevachari MB; Dewar RL; Stephens A; Psallidopoulos MC; Salzman NP; Georgetown University, Washington, DC; OBJECTIVE: To study the structure-function relationship of envelope glycoproteins of HIV-1 made in wild-type and glycosylation mutant CHO cell lines. METHODS: HIV 1 envelope glycoproteins were produced in wild-type and lectin resistant CHO cell lines infected with a recombinant human adenovirus which carries the HIV-1
HIV modulation of cell surface glycoprotein expression.: Int Conf AIDS. 1991 Jun 16-21;7(1):136 (abstract no. M.A.1177). Unique Identifier : AIDSLINE ICA7/1117791; Mosca JD; Rosenberg Y; Perera P; White B; D'Arcy L; Ritchey D; Burke DS; Henry M. Jackson Foundation, Rockville, MD; OBJECTIVE: To examine the correlation between CD4 depletion and HIV expression. METHODS: Chronically HIV-1 infected cell lines were established using virus derived from a molecular clone. Cell-free media from ten of these lines, in addition to a series of HIV-1 isolates, were tested on SupT1 cells to modulate the expr
Expression of the nef gene of HIV-1 by transfection into B-cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):136 (abstract no. M.A.1178). Unique Identifier : AIDSLINE ICA7/1117891; Kienzie N; Muller-Lantzsch N; University of the Saarland, Dept. Virology, Homburg, FRG; OBJECTIVE: Establishment of B-cell clones stably transfected with the nef gene for investigation of interactions of nef with cellular components. METHODS: Using a transfection vector which contained the essential elements of EBV for episomal replication and the selectable hygromycin B marker, the nef gene of HIV-1 was
Simultaneous post-embedding ultrustructural detection of RNA and proteins of HIV-I.: Int Conf AIDS. 1991 Jun 16-21;7(1):136 (abstract no. M.A.1179). Unique Identifier : AIDSLINE ICA7/1117991; Grigoriev V; Escaig-Haye F; Fournier JG; Sharova N; Tugizov SH; Klimenko S; The D. I. Ivanovsky Institute of Virology, Acad. Med. Sci.,; Moscow, USSR; OBJECTIVE: The purpose of this study was to detect and localize HIV antigens and its RNA molecules simultaneously in infected cells during morphogenesis of virus at the ultrastructural level. METHODS: Jurkat-tat cells chronically infected with HIV-I, strain A-899, were fixed and embedded in Lowicryl K4M resin with the
Mechanism of function of HIV rev and HTLV rex.: Int Conf AIDS. 1991 Jun 16-21;7(1):137 (abstract no. M.A.1180). Unique Identifier : AIDSLINE ICA7/1118091; Benko DM; Unge T; Solomin L; Ciminale V; Tavernarakis N; Paviakis GN; Felber BK; Basic Research Program National Cancer Institute-FCRDC,; Frederick, MD, USA; OBJECTIVE: To study the mechanism of activation of viral structural gene expression by the regulatory proteins Rev and Rex. RESULTS AND DISCUSSION: Rev, mutant Rev, and Rex proteins were expressed in bacteria, purified, and analysed for their ability to bind to the cis-acting Rev responsive element (RRE) and Rex-respo
Expression of immunologically reeactive recombinant tat, rev and tev proteins of HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):137 (abstract no. M.A.1181). Unique Identifier : AIDSLINE ICA7/1118191; Tavernarakis N; Pavlakis GN; Krambovitis E; Institute of Molecular Biology and Biotechnology, Heraklion,; Crete, Greece; OBJECTIVE: To express the regulatory proteins tat, rev and the recently identified tev in bacteria for the study of patient immune responses to these proteins. RESULTS AND DISCUSSION: The proteins encoded by the tat and rev genes are necessary for HIV-1 replication and are currently the subject of intensive studies. W
Critical sequences responsible for virion formation of HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):137 (abstract no. M.A.1182). Unique Identifier : AIDSLINE ICA7/1118291; Hoshikawa N; Masuko S; Yasuda A; Takayashiki E; Kojima A; Kurata T; Kosei Hospital, Tokyo, Japan; OBJECTIVE: The gag precursor polyprotein of HIV-1 assembles spontaneously into retrovirus-like particles at the cell membrane. To clarify the critical sequence responsible for viral assembly, we constructed recombinant vaccinia viruses (RVVs) containing a variety of truncated gag genes of HIV-1. METHODS: Oligonucleoti
Essential role of N-myristoylation of gag protein in budding and assembly of HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):137 (abstract no. M.A.1183). Unique Identifier : AIDSLINE ICA7/1118391; Kojima A; Hoshikawa N; Yasuda A; Takayashiki E; Sata T; Kurata T; National Institute of Health, Tokyo, Japan; OBJECTIVE: We have previously shown that the HIV gag polyprotein precursor is able to assemble into virus-like particle without other viral proteins. The N-terminal glycine of gag protein is myristoylated co-translationally. To investigate the functional significance of the modification, we deleted the glycine codon f
Fine mapping of the immunodominant domains of the HIV-1 and HIV-2 integrase proteins.: Int Conf AIDS. 1991 Jun 16-21;7(1):138 (abstract no. M.A.1184). Unique Identifier : AIDSLINE ICA7/1118491; Vinga-Martins C; Schneider T; Roenspeck W; Pauli G; Mueller-Lantzsch N; University of the Saarland, Dept. Virology, Homburg, FRG; OBJECTIVE: Fine mapping of the major immunodominant epitopes of the HIV-1 and HIV-2 integrase proteins by synthetic oligopeptides and recombinant proteins. METHODS: Overlapping decapeptides spanning the entire HIV-1 and HIV-2 integrase proteins were used as synthetic antigens in a pin-based oligopeptide-ELISA to test
Characterization of VPU from HIV-1 expressed in E.coli and mammalian cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):138 (abstract no. M.A.1185). Unique Identifier : AIDSLINE ICA7/1118591; Schubert U; Morelle C; Hoffmann K; Hauser H; Schneider T; Pauli G; Porstmann T; Dep. of Med. Immunology, Med. School (Charite) Humboldt Univ.; Berlin (HUB); The 16 KD vpu(out) protein is only found in HIV-1 infected cells at very low concentrations. It is thought to be a phosphorylated membrane protein which has a positive effect on budding and assembling of the virus. We expressed the vpu-gene after isolation by PCR in E.coli as an unfused protein by using a two-cistron
Oligomeric status, carbohydrate contents, and molecular weights of HIV-1 glycoproteins.: Int Conf AIDS. 1991 Jun 16-21;7(1):138 (abstract no. M.A.1186). Unique Identifier : AIDSLINE ICA7/1118691; Steven A; Trus B; Booy F; Kaczorek M; Wall J; Hainfeld J; Eiserling F; Thomas D; National Institutes of Health, Bethesda, MD, USA; OBJECTIVE: With the aim of resolving enduring ambiguities as to certain basic molecular properties of the env glycoproteins, we have applied quantitative electron microscopy and image processing, including scanning transmission electron microscopy (STEM) of unstained specimens, to purified gp160 and gp120. METHODS: Re
In vitro sperm mediated HIV proviral DNA transfer to lymphoblastoid cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):138 (abstract no. M.A.1187). Unique Identifier : AIDSLINE ICA7/1118791; Fazio VM; Rinaldi M; Catani MV; Ravagnan G; Metafora S; Manna C; Peluso G; Farace MG; Institute of Experimental Medicine, CNR, Rome, Italy; OBJECTIVE: Our group has recently shown that spermatozoa can take up exogenous DNA which is transferred to egg cells at fertilization. Moreover, several reports have demonstrated that human spermatozoa bind and penetrate HLA-DR+ leukocytes. We report here that human spermatozoa can capture in vitro HIV proviral DNA, a
Helical polymers of HIV-1 REV protein.: Int Conf AIDS. 1991 Jun 16-21;7(1):139 (abstract no. M.A.1188). Unique Identifier : AIDSLINE ICA7/1118891; Misra M; Steven A; Booy F; Kocsis E; Wingfield P; National Institute of Arthritis, Musculoskeletal and Skin; Diseases; OBJECTIVE: In order to understand how REV regulates the expression of the ENV gene by binding to the REV-responsive element of ENV mRNA, thereby inhibiting splicing, we are studying the properties of REV protein expressed in E.coli. We have found that, under appropriate in vitro conditions, purified REV polymerizes in
Effective suppression of HIV replication in vitro by combination treatment with AZT and recombinant interferon-alpha.: Int Conf AIDS. 1991 Jun 16-21;7(1):139 (abstract no. M.A.1189). Unique Identifier : AIDSLINE ICA7/1118991; Epstein J; Joshi B; Lee S; Pollock L; Mayner R; Hewlett I; Laboratory of Retrovirology, DTS, CBER, FDA, Bethesda, MD USA; Introduction; We have evaluated the efficacy of the antiviral agents, AZT and recombinant interferon-alpha (rIFN-a) both singly and in combination, to inhibit the expression of HIV-1 at the nucleic acid level using PCR. METHODS: H9 and U937 cells were
Induction of HIV-1 from chronically infected promonocytic cells by chemical carcinogens.: Int Conf AIDS. 1991 Jun 16-21;7(1):139 (abstract no. M.A.1190). Unique Identifier : AIDSLINE ICA7/1119091; Hewlett I; Joshi B; Riordan G; Lee S; Pollock L; Epstein J; Laboratory of Retrovirology, DTS, FDA, Bethesda, MD, USA; OBJECTIVE: To evaluate the effect of chemical carcinogens on the expression of HIV in chronically infected monocytic cells. METHODS: The chronically infected promonocytic cell line U1 is characterized by low constitutive HIV production, which is induced by phorbol esters (TPA) and tumor necrosis factor (TNF-a). Cells
HIV-1 integrase expressed as a fusion protein in E.coli exhibits DNA binding and endonuclease activity.: Int Conf AIDS. 1991 Jun 16-21;7(1):139 (abstract no. M.A.1191). Unique Identifier : AIDSLINE ICA7/1119191; Marcus-Sekura C; Woerner A; LDNAV, DV, Center for Biologics Evaluation and Research, FDA,; Bethesda, MD USA; OBJECTIVE: To determine whether HIV-1 integrase (IN) (the viral protein required for integration of reverse transcribed virus DNA into the host cell genome), when expressed as a fusion protein in E. coli, exhibits properties expected of a biologically active molecule. METHODS: Several IN expressing clones have been co
Human monocytes/macrophages (HMM) HIV-1 infection: TNFalpha production, cell activation and microbicide activity.: Int Conf AIDS. 1991 Jun 16-21;7(1):140 (abstract no. M.A.1192). Unique Identifier : AIDSLINE ICA7/1119291; Le Naour R; Raoul H; Mabondzo A; Ripoll L; Barre-Sinoussi FC; Caire Y; Dormont D; SSA, CEA, Fontenay-aux-Roses; OBJECTIVE: To determine 1) the consequence of HIV infection on the TNFalpha synthesis and regulation, 2) the relationships between HIV infection and the different activation stages of the HMM by analysing the expression of the 1-6 fructose-biphosphatase (1-6 FBPasc) gene, and 3) the capacity of the HIV infected HMM to
Assembly domain of the HIV-1 envelope protein.: Int Conf AIDS. 1991 Jun 16-21;7(1):140 (abstract no. M.A.1193). Unique Identifier : AIDSLINE ICA7/1119391; Earl PL; Moss B; Laboratory of Viral Diseases, National Institute of Allergy and; Infectious Diseases, Bethesda, MD 20892, USA; OBJECTIVE: To examine the assembly domain of the HIV-1 envelope (env) glycoprotein. METHODS: Env protein was produced via infection of cells with a recombinant vaccinia virus expressing the bacteriophage T7 RNA polymerase gene and transfection with plasmids containing secreted forms of the env gene under control of a
HIV-I noninfectious virus-like particles interfere with virions.: Int Conf AIDS. 1991 Jun 16-21;7(1):140 (abstract no. M.A.1194). Unique Identifier : AIDSLINE ICA7/1119491; Vzorov A; Bukrinskaya A; Central Institute of Advanced Training of Physicians, Moscow,; USSR; OBJECTIVE: Noninfectious HIV-I virus-like particles were obtained by infection of CV-I cells with two vaccinia viruses containing gag and env genes respectively. It was important to know whether the particles were able to interact with lymphoid cells and to interfere with infectious virions. METHODS: HIV-I produced by
Production of highly immunogenic recombinant HIV-1 integration protein (INT) by recombinant vaccinia virus and E. coli.: Int Conf AIDS. 1991 Jun 16-21;7(1):140 (abstract no. M.A.1195). Unique Identifier : AIDSLINE ICA7/1119591; Tachibana Y; Kojima A; Kobayashi T; Yasuda A; Sata T; Kurata T; NIPPON ZEON Co., Ltd., Kanagawa, Japan; OBJECTIVE: As a more precise diagnostic alternative to the present analysis of HIV-1 p24, gp41, gp160, p66, p55, etc., in HIV-infected individuals, and to explore the possibility of the anti-HIV drugs which block the integration stage of the HIV-1 life cycle, we produced recombinant INT with both recombinant vaccinia
Natural HIV-1 3'-ORF acts as a positive regulator for viral replication in primary human lymphocytes.: Int Conf AIDS. 1991 Jun 16-21;7(1):141 (abstract no. M.A.1196). Unique Identifier : AIDSLINE ICA7/1119691; De Ronde A; Klaver B; Keulen W; Goudsmit J; Human Retrovirus Laboratory, University of Amsterdam,; Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands; OBJECTIVE: To elucidate the function of HIV-1 3 -ORF in an environment mimicking as closely as possible its natural one. METHODS: Six HIV-1 3 -ORF sequences were isolated from HIV-1 infected individuals by nested PCR and cloned in an HXB-2 molecular clone background. Chimeric molecular clones were transfected into T-c
Heptameric CD4-C4bp fusion proteins efficiently block HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):141 (abstract no. M.A.1197). Unique Identifier : AIDSLINE ICA7/1119791; Pasek M; Burrus B; Crimmins M; Kirchhausen T; Liu T; Miatkowski K; Meier W; Turner S; Winkler G; Biogen, Inc., Cambridge, MA, USA; We have constructed heptameric CD4 molecules, CD4(1-187)-C4bp(4SCR), by fusing the N-terminal 187 amino acids from human CD4 to proline 248 at the amino terminal edge of the fourth short consensus repeat of human complement 4 binding protein. We have expressed this molecule both in COS and CHO cells. The polypeptide c
Kynetics of HHV-6 transactivation of HIV-1 LTR and effect of HIV-1 tat on HHV-6 replication.: Int Conf AIDS. 1991 Jun 16-21;7(1):141 (abstract no. M.A.1198). Unique Identifier : AIDSLINE ICA7/1119891; Di Luca D; Secchiero P; Bovenzi P; Cassai E; Institute of Microbiology, University of Ferrara, Ferrara, Italy; OBJECTIVE: This research focused upon: 1) the kynetics of HHV-6 transactivation of HIV LTR and 2) the interactions between HHV-6 and tat. METHODS: The kynetics was studied in the human T cell line H938, containing the reporter gene CAT driven by the HIV LTR. The interactions between HHV-6 and tat were studied in clone
Molecular cloning of the gene for a cryptosporidium sporozoite surface protein.: Int Conf AIDS. 1991 Jun 16-21;7(1):142 (abstract no. M.A.1200). Unique Identifier : AIDSLINE ICA7/1120091; Petersen C; Gut J; Leech JH; Nelson RG; Parasitology Laboratory, San Francisco General Hospital and; Departments of Medicine and Pharmaceutical Chemistry, University; of California, San Francisco, CA, USA; OBJECTIVE: To clone the gene for a surface glycoprotein of Cryptosporidium sporozoites that is the target of a monoclonal antibody (Mab 10C6) that reacts with intracellular merozoites and the surface of sporozoites, causes shedding of the sporozoite surface coat and inhibits sporozoite invasion of Mardin-Darby Canine
Differential immunological features in PCR-HIV+ and PCR-HIV- hemophilia A patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):142 (abstract no. M.A.1201). Unique Identifier : AIDSLINE ICA7/1120191; Cristiana C; Gringeri A; Macioce G; Vulcano F; Mariani G; Testa U; Bulgarini D; Mannucci PM; Hassan HJ; Peschle C; Dept. of Hematology-Oncology, Istituto Superiore di Sanita,; Rome, Italy; OBJECTIVE: Polymerase Chain Reaction (PCR) technique was used to analyze the presence of HIV-1 sequences in DNA from two matched groups of 26 seronegative and 18 seropositive hemophilia A patients, equally exposed in the 1981-85 period to not virus-inactivated FVIII concentrates (greater than 75% of the batches coinci
The cleavage of the bait region of alpha2-macroglobulin (alpha2M) by HIV-1 protease (PR).: Int Conf AIDS. 1991 Jun 16-21;7(1):142 (abstract no. M.A.1202). Unique Identifier : AIDSLINE ICA7/1120291; Billich A; Mann K; Meier UC; Schramm HJ; Sandoz-Forschungsinstitut, Vienna, Austria; OBJECTIVE: The specificity of PR is known from the cleavage of the multiprotein of HIV and from other cleavages. The prevalent opinion is that only extended inter-domain segments are cleaved. Since alpha2M contains an accessible bait region we used it to test this assumption. A second question was whether the C-termin
HIV-1 protease (PR) cleaves calmodulin (CaM) and the alpha chain of phosphorylase-kinase (PK).: Int Conf AIDS. 1991 Jun 16-21;7(1):142 (abstract no. M.A.1203). Unique Identifier : AIDSLINE ICA7/1120391; Billich A; Daube H; Mann K; Schramm HJ; Sandoz-Forschungsinstitut, Vienna, Austria; OBJECTIVE: The specificity of PR is known from the cleavage of the pol-gag polyprotein of HIV and from other cleavages found in multi-domain proteins. Since PR may also act on cellular proteins, it is necessary to learn more about the conditions of specific cleavage. PK, a large multi-protein complex was used as subst
Does the HIV nef protein bind guanine nucleotides?: Int Conf AIDS. 1991 Jun 16-21;7(1):142 (abstract no. M.A.1204). Unique Identifier : AIDSLINE ICA7/1120491; Wolber V; Rensland H; Brandmeier B; Wittinghofer F; Kalbitzer HR; Max-Planck-Institute for Medical Research, Heidelberg, FRG; OBJECTIVE: Biochemical and biophysical characterisation of recombinant nef (negative factor) gene product. METHODS: The nef gene of HIV-1, isolate LAV1, was expressed in E.coli under the control of the tac promotor. The recombinant protein is found mainly in the soluble fraction of the bacterial lysate, therefore the
Direct sequencing as a tool for HIV-1 genotyping.: Int Conf AIDS. 1991 Jun 16-21;7(1):143 (abstract no. M.A.1205). Unique Identifier : AIDSLINE ICA7/1120591; Salminen M; Leinikki P; Ayehunie S; Johansson B; Strannegard O; HIV-Unit, National Public Health Institute, Helsinki, Finland; OBJECTIVE: To develop a phylogenetic tree analysis that can be applied to epidemiological and pathogenesis studies. METHODS: Three primer nested PCR was used to amplify regions of HIV-1 from uncultured human white blood cells. The primers were selected from conserved regions of the gag and env genes. Biotin was incorp
Impaired HIV replication in CEMss cells bearing a non-producer HIV genome endowed with homologous viral interference.: Int Conf AIDS. 1991 Jun 16-21;7(1):143 (abstract no. M.A.1206). Unique Identifier : AIDSLINE ICA7/1120691; Federico M; Taddeo B; Titti F; Carlini F; Orecchia A; Verani P; Rossi GB; Lab. Virology, Istituto Superiore di Sanita, Rome, Italy; OBJECTIVE: To demonstrate if a non-producer HIV-1 genome inserted in a retroviral vector and transferred in human CD4+ cells is able to inhibit the replication of a super-infecting HIV-1. METHODS: An Hut-78 cell clone (F12) infected by a non-producer HIV-1 variant showed a complete resistance to HIV-1, HIV-2 or SIV su
Sequence analysis of an HIV-1 proviral DNA from a non producer chronically infected HUT-78 cellular clone.: Int Conf AIDS. 1991 Jun 16-21;7(1):143 (abstract no. M.A.1207). Unique Identifier : AIDSLINE ICA7/1120791; Carlini F; Federico M; Equestre M; Ricci S; Ratti G; Verani P; Rossi GB; Lab. Virology, Istituto Superiore di Sanita, Rome, Italy; OBJECTIVE: To correlate the peculiar features of a non producer HIV-1-infected cell clone (F12) (Federico at al. AIDS Res Hum Retr, 1989, 5: 385) with possible genomic mutations. F12 clone, in spite of the presence of an integrated full-length HIV-1 provirus, does not release virus particles and shows an altered prote
Cleavage of HIV mRNA by hammerhead ribozymes.: Int Conf AIDS. 1991 Jun 16-21;7(1):144 (abstract no. M.A.1208). Unique Identifier : AIDSLINE ICA7/1120891; Crisell P; James W; Sir William Dunn School of Pathology, University of Oxford, UK; OBJECTIVE: To produce and analyse hammerhead-model ribozymes that cleave HIV mRNA; to establish optimum conditions for cleavage in vitro; to analyse the relationship between flanking sequence length and kinetic properties; to compare in vitro cleavage with anti-HIV activity in vivo. METHODS: 22 bases corresponding to
Neutralization loop variability in HIV-1 isolates from the Kinshasa area.: Int Conf AIDS. 1991 Jun 16-21;7(1):144 (abstract no. M.A.1209). Unique Identifier : AIDSLINE ICA7/1120991; Guo HG; Zagury D; Waters D; Jagodzinski L; Fitzgibbon J; Gallo RC; Reitz M; Lofstrand Laboratories, Gaithersburg, MD, USA; We analyzed a central portion of the env gene of a number of isolates of HIV-1 collected in 1987 from Kinshasa, Zaire and its surrounding regions. The env gene sequences were generated by PCR and included the PB1 loop, a region which is frequently recognized by type specific neutr
Use of synthetic genetic bar codes in polymerase chain reaction to facilitate the study of HIV genetic variability.: Int Conf AIDS. 1991 Jun 16-21;7(1):144 (abstract no. M.A.1210). Unique Identifier : AIDSLINE ICA7/1121091; Ou CY; Luo CC; Bandea C; Villamarzo Y; Moore JL; de la Torre N; Schochetman G; Centers for Disease Control, Atlanta, GA, USA; OBJECTIVE: To facilitate sequence determination of HIV. METHODS: HIV sequences from the peripheral blood mononuclear cells (PBMCs) of infected persons were first amplified through polymerase chain reactions (PCR). Small aliquots of the amplified products were reamplified using a second primer pair with extended sequen
A possible role of the p18 part of gag in variation of HIV-1 cytopathic effect.: Int Conf AIDS. 1991 Jun 16-21;7(1):144 (abstract no. M.A.1211). Unique Identifier : AIDSLINE ICA7/1121191; de Mareuil J; Salaun D; Kutinova L; Vonka V; Chermann JC; Hirsch I; INSERM Unite 322, Marseille, France; OBJECTIVE: To elucidate a role of genetic elements localized in HIV1 NDK fragment BssHII/Spel (nt 255-1042) for HIV1 cytopathogenicity. METHODS: Recombinant provirus molecules between HIV1 BRU and HIV1 NDK were constructed using conservative BssHII, Spel, EcoRI and Xhol sites. HIV1 NDK derived fragment BssHII/Spel was
The HIV-I nef gene product inhibits translation in vitro.: Int Conf AIDS. 1991 Jun 16-21;7(1):145 (abstract no. M.A.1212). Unique Identifier : AIDSLINE ICA7/1121291; Poulin L; Darveau A; Levy JA; Centre Hospitalier de l'Universite Laval, Quebec, Qc, Canada; OBJECTIVE: To analyze the function of HIV-1 nef gene product in the negative regulation of HIV replication. METHODS: The HIV-1SF2 nef gene has been subcloned into a transcription vector and used to produce a nef mRNA. The generated messenger has then been translated into a micrococcal nuclease-treated reticulocyte lys
Absence of truncated transmembrane protein in two macaques at the terminal stage of the disease.: Int Conf AIDS. 1991 Jun 16-21;7(1):145 (abstract no. M.A.1213). Unique Identifier : AIDSLINE ICA7/1121391; Fossati I; Courgnaud V; Brechot C; Sonigo P; Institut Cochin de Genetique moleculaire; OBJECTIVE: Presence of a stop codon that truncates the transmembrane protein (TMP) of HIV and SIV isolates has been described by different authors. The truncated form, in the case of SIV isolate has only been found until now, in vitro, after culture in human PBMC or human CD4 positive cells. As rapid high variants of
Sequence comparison of two SIVSMM isolates with different biological properties.: Int Conf AIDS. 1991 Jun 16-21;7(1):145 (abstract no. M.A.1214). Unique Identifier : AIDSLINE ICA7/1121491; Courgnaud V; Laure F; Montagnier L; Fultz P; Brechot C; Sonigo P; U75 Necker, ICGM Genetique des virus, Diagnostics Pasteur,; Institut Pasteur, Paris, France; OBJECTIVE: To compare the nucleotide sequence of a lethal infectious molecular clone derived from isolate SIV/SMM-Pbj14 (Dewhurst et al) with the sequence of the original non pathogenic isolate SIV/SMM-9. SIV/SMM-Pbj14 was generated by mutation of SIV/SMM-9 after only one passage in vivo (Fultz et al). METHODS: Entire
Mutations in the HIV-1 rev nuclear localisation signal abrogate binding to rev responsive element (RRE) RNA.: Int Conf AIDS. 1991 Jun 16-21;7(1):145 (abstract no. M.A.1215). Unique Identifier : AIDSLINE ICA7/1121591; Berger J; Aepinus C; Dubrovnik M; Kreusel J; Fleckenstein B; Hauber J; Boehniein E; SANDOZ Resarch Institute, Vienna, Austria; HIV-1 replication depends on the expression of trans-regulatory genes (tat, rev) encoded in the 3 part of the retroviral genome. HIV-1 tat protein augments retroviral gene expression on a transcriptional and post-transcriptional level. Rev is required for the cytoplasmic translocation of incompletely-spliced retrovira
Investigation of HIV-1 packaging.: Int Conf AIDS. 1991 Jun 16-21;7(1):146 (abstract no. M.A.1216). Unique Identifier : AIDSLINE ICA7/1121691; Harrison G; Lever A; Communicable Diseases Unit, St George's Hospital Medical School,; Cranmer Terrace, London. SW17 ORE. UK; OBJECTIVE: Determination of the secondary structure of the packaging signal of HIV-1. The full-length unspliced mRNA of retroviruses codes for the gag and pol genes and is also the species packaged as a dimer into the exported virion particles as the genome RNA. Sequences essential for efficient packaging of this RNA
Biosynthesis and processing of the human immunodeficiency virus type 1 structural proteins in infected cells: the limits for virus growth.: Int Conf AIDS. 1991 Jun 16-21;7(1):146 (abstract no. M.A.1217). Unique Identifier : AIDSLINE ICA7/1121791; Zaides VM; Selimova LM; Klyushnik SY; Veselovskaya TV; Rikova LA; The D.I. Ivanovsky Institute of Virology, Moskow, 123093, USSR; The synthesis and metabolism of HIV-1 structural 35S-proteins in persistently infected cells were studied by pulse-chase protocol. The primary translational products, pr180 (gag-pol genes fusion protein), gp160 (env) and p55 (gag) were synthesized at the relative molar rates of approximately 1:50:80, and the rates and
Ribozymes as anti-HIV agents: insertion of cis and trans ribozymes into the HIV-genome.: Int Conf AIDS. 1991 Jun 16-21;7(1):146 (abstract no. M.A.1218). Unique Identifier : AIDSLINE ICA7/1121891; Lin NH; Dropulic B; Graham K; Jeang KT; Laboratory of Molecular Microbiology, NIAID, NIH, Bethesda, MD,; USA; OBJECTIVE: To study the potential of ribozymes as anti-HIV agents. METHODS: Standard molecular biology techniques were used. RESULTS: Certain RNA molecules, called ribozymes, display enzymatic activities. Their unique secondary structure and sequence enable highly specific cleavage reactions. Two classes of ribozymes
Extracellular interaction of HHV-6 replication products on HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):146 (abstract no. M.A.1219). Unique Identifier : AIDSLINE ICA7/1121991; Ongradi J; Ceccherini-Nelli L; Pistello M; Specter S; Friedman H; Bendinelli M; Institute of Microbiology, Semmelweis University of Medicine,; Budapest, Hungary; A potentiating effect, likely of the early gene products, of heterogeneous coinfections by several viruses, among them human herpesvirus type 6 /HHV-6/ (cytopathic on permissive cells such as CD4+ lymphocytes) on HIV-1 replication is well documented in vitro: leading to enhanced transcription, antigenic expression and
Lysophagosome fusion of macrophages from HIV infected patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):147 (abstract no. M.A.1220). Unique Identifier : AIDSLINE ICA7/1122091; Estevez ME; Pittis G; Iannitelli P; Planes N; Pirola D; Sen L; Academia Nacional de Medicina de Buenos Aires, Buenos Aires,; Argentina; OBJECTIVE: Previously we found that peripheral blood monocytes from HIV infected individuals have an impairment to lyse candidas with a reduction in the generation of toxic oxygen metabolites. The aim of the study was to evaluate the monocytic lysophagosome fusion, as a previous step of the oxygen independent microbic
Genetic risk factors for clinical manifestation in pediatric HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):147 (abstract no. M.A.1221). Unique Identifier : AIDSLINE ICA7/1122191; Stein Z; Abrams E; Just J; Louis L; Nicholas S; King MC; HIV Center for Clinical & Behavioral Studies, NYS Psychiatric; Institute & Columbia University, New York, NY, USA; OBJECTIVE: To search for associations between clinical manifestation of illness and genotype of candidate sequences among infants found with active HIV infection following maternal transmission of the virus. METHODS: 40 Black American infants infected through the maternal route, all with established HIV infection, wer
Phagocytosis of mycobacterium tuberculosis stimulates transcription of human immunodeficiency virus (HIV) types 1 and 2 in macrophages.: Int Conf AIDS. 1991 Jun 16-21;7(1):147 (abstract no. M.A.1222). Unique Identifier : AIDSLINE ICA7/1122291; Shattock R; Friedland JS; Griffin GE; Division of Communicable Diseases, Department of Cellular and; Molecular Sciences, St. George's Hospital Medical School, Cranmer; Terrace, London, SW17 ORE, England; OBJECTIVE: HIV infects human macrophages and may remain latent for years before development of the Acquired Immunodeficiency Syndrome (AIDS). We have investigated the possibility that phagocytosis of a pathogen, in particular M. tuberculosis known to be important in clinical AIDS, leads to activation of HIV. METHODS:
Identification and characterization of human herpes virus 6 gene segment that transactivate HIV.: Int Conf AIDS. 1991 Jun 16-21;7(1):147 (abstract no. M.A.1223). Unique Identifier : AIDSLINE ICA7/1122391; Wood C; Geng Y; Balachandran N; University of Kansas, Lawrence, KS, USA; OBJECTIVE: Human herpes virus 6 (HHV-6) is a recently isolated lymphotropic herpes virus. HHV-6 can productively co-infect HIV-infected CD4+ T cells. It has been demonstrated that co-infection by both viruses can lead to accelerated cytopathic effects when compared to HIV or HHV-6 infection alone. The objective of the
Neutralizing monoclonal antibodies to HHV6 identify virion envelope glycoproteins.: Int Conf AIDS. 1991 Jun 16-21;7(1):148 (abstract no. M.A.1224). Unique Identifier : AIDSLINE ICA7/1122491; Campadelli-Fiume G; Boscaro A; Di Gaeta S; Foa-Tomasi L; The Section of Microbiology and Virology, Department of; Experimental Pathology, University of Bologna, Bologna, Italy.; OBJECTIVE: To characterize HHV6 glycoproteins and to infer their functions through the production of MAbs. METHODS: BALB/c mice were immunized with lysates of cord blood lymphocytes infected with HHV6(U1102). Antibody secreting hybridomas were identified by ELISA and IFA. The reactive proteins were characterized by im
Association between seminal white blood cells and HIV-1 in semen.: Int Conf AIDS. 1991 Jun 16-21;7(1):148 (abstract no. M.A.1225). Unique Identifier : AIDSLINE ICA7/1122591; Politch J; Martinez A; Abbott A; Mayer K; Padian N; Seage G; O'Brien T; Horsburgh C; Anderson D; Harvard Univ., Boston MA; OBJECTIVE: Seminal white blood cells (wbc) have been implicated as vectors in the sexual transmission of HIV-1. The purpose of this study was to characterize wbc in semen from HIV-1 seropositive men, and to determine if wbc types, concentrations and/or activation status are associated with a positive HIV-1 semen cultu
Complexity of genetic susceptibility of human cells to HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):148 (abstract no. M.A.1226). Unique Identifier : AIDSLINE ICA7/1122691; Cambiaggi C; Ramarli D; Mantero G; Primi D; Tridente G; Accolla RS; Istituto di Scienze Immunologiche e Servizio di Immunopatologia,; Universita di Verona, Policlinico Borgo Roma, Verona, Italy; The HIV specific receptor is the CD4 molecule. However CD4 is necessary but not sufficient for virus full infecting capacity as demonstrated by the fact that not all CD4- cells in HIV+ individuals are infected and that murine cells transfected with human CD4 bind the virus but do not get infected. This suggests that o
HHV6 (human herpesvirus 6) in lymphomas and HIV infected hemophiliacs.: Int Conf AIDS. 1991 Jun 16-21;7(1):148 (abstract no. M.A.1227). Unique Identifier : AIDSLINE ICA7/1122791; Ceccherini-Nelli L; Torelli G; Marasca R; Luppi M; Pistello M; Ghilarducci R; Batoni G; Cecconi N; Panicucci F; Bendinelli M; University of Pisa, Pisa, Italy; OBJECTIVE: To assess the degree of association of HHV6 active infection with Hodgkin s (Hg) and non Hodgkin s (nHg) lymphomas and with HIV-infected hemophilic patients: HHV6 has been proposed as a potential cofactor in the onset and/or development of these lymphoproliferative disorders and of AIDS. METHODS: Sera from
Hepatitis B virus (HBV) X protein acts through NF-kB transcription factor: a possible mechanism for HBV-induced stimulation of a latent HIV genome.: Int Conf AIDS. 1991 Jun 16-21;7(1):149 (abstract no. M.A.1228). Unique Identifier : AIDSLINE ICA7/1122891; Natoli G; Balsano C; Avantaggiati MI; De Marzio E; Elfassi E; Levrero M; Fond. A. Cesalpino - 1 Clinica Medica - University of Rome -; Italy; OBJECTIVE: HBV might act as a cofactor in the development and/or progression of the acquired immunodeficiency syndrome (AIDS). The aim of this study is to understand the molecular basis of the interaction between HBV and HIV. METHODS: We performed several cotransfection experiments in which plasmid vectors expressing
Activation of the HIV promoter by radiation and drugs.: Int Conf AIDS. 1991 Jun 16-21;7(1):149 (abstract no. M.A.1229). Unique Identifier : AIDSLINE ICA7/1122991; Zmudzka BZ; Beer JZ; Olvey KM; Strickland AG; Lee W; Jacobs ME; Food and Drug Administration, HFZ-114, Rockville, MD 20857, USA; OBJECTIVE: Several laboratories reported that HIV is activated by ultraviolet radiation (UV). We evaluated the HIV-activating potential of radiation exposures used in dermatology, oncology, in experimental AIDS treatment, for experimental blood product cleansing, and in cosmetology. Such exposures involve ultraviolet
Human hematopoietic stem cell toxicity associated with zidovudine in vitro: effects of G-CSF and M-CSF.: Int Conf AIDS. 1991 Jun 16-21;7(1):149 (abstract no. M.A.1230). Unique Identifier : AIDSLINE ICA7/1123091; Gallicchio V; Hughes N; Lucille P. Markey Cancer Center, University of Kentucky Medical; Center, Lexington, KY, USA; OBJECTIVE: To investigate the capacity of G-CSF and M-CSF to ameliorate the hematopoietic toxicity associated with zidovudine following co-culture studies with human bone marrow cells in vitro. METHODS: Adherent and T-cell depleted normal human bone marrow cells were incubated in vitro either in the presence or absenc
Hepatitis B virus infection of peripheral blood lymphocytes in transcriptionally active HIV-1 infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):149 (abstract no. M.A.1231). Unique Identifier : AIDSLINE ICA7/1123191; Manzin A; Bagnarelli P; Menzo S; Valenza A; Pauri P; Carloni G; Clementi M; Institute of Microbiology, University of Ancona; OBJECTIVE: To verify whether presence of hepatitis B virus DNA in PBLs of HIV-1 infected patients correlates with transcriptionally active HIV-1 infection. METHODS: DNAs and RNAs were extracted from nuclei and cytoplasmic fractions, respectively, of PBLs from 5 ml of blood of HIV-1 seropositive individuals. HBV DNA se
In vitro interactions between HIV and trichomonas vaginalis.: Int Conf AIDS. 1991 Jun 16-21;7(1):150 (abstract no. M.A.1232). Unique Identifier : AIDSLINE ICA7/1123291; Dolei A; Serra C; Mattana A; Juliano C; Arca MV; Cappuccinelli P; Institute of Microbiology and Virology, University of Sassari,; Italy; OBJECTIVE: The aim of this study was to evaluate the possibility that Trichomonas vaginalis (T.v.), responsible for a worldwide diffused sexually-transmitted disease, may behave as a carrier for heterosexual transmission of HIV infection. To this purpose in vitro experiments were devised in order to study the interact
EBV reactivation in HIV-1 superinfected lymphoblastoid cell lines spontaneously established from HIV seropositive individuals.: Int Conf AIDS. 1991 Jun 16-21;7(1):150 (abstract no. M.A.1233). Unique Identifier : AIDSLINE ICA7/1123391; Ottmann M; Innocenti P; Morand P; Seigneurin JM; Laboratoire de Virologie, Faculte de medecine, Grenoble, France; OBJECTIVE: To study HIV and EBV interactions, by investigating both virus replication in in vitro HIV superinfected EBV positive Lymphoblastoid Cell Lines (LCL). METHODS: Seven spontaneous LCL from HIV seropositive individuals were infected in vitro with two different strains of HIV-1 (HIVLAV-Bru and a strain isolated
HIV-1 enhances intracellular growth of mycobacterium avium in human macrophages.: Int Conf AIDS. 1991 Jun 16-21;7(1):150 (abstract no. M.A.1234). Unique Identifier : AIDSLINE ICA7/1123491; Hoffner SE; Kallenius G; Koivula T; Rydgard KJ; Valentin A; Asjo B; Ljungh C; Svenson SB; National Bacteriological Laboratory, S-105 21 Stockholm, Sweden; OBJECTIVE: Disseminated Mycobacterium avium infections are common in AIDS patients and result in a reduced life expectancy. Human monocytes/macrophages are important target cells both for HIV and M avium. The objective of this study was to evaluate in vitro the interaction of M avium and HIV-1 in human macrophages. ME
Human herpesvirus-6 and HIV infection in Africa and in Europe.: Int Conf AIDS. 1991 Jun 16-21;7(1):150 (abstract no. M.A.1235). Unique Identifier : AIDSLINE ICA7/1123591; Ranger S; Weinbreck P; Sangare A; M'Boup S; Souquiere S; Denis F; Virology Department, CHU Limoges, France; HHV-6 was first thought to be a cofactor in disease caused HIV infection, then it was shown to suppress near totally the replication of HIV-1. OBJECTIVE: To examine the differences in expression of HHV-6 in HIV-1 or 2 antibody positive individuals with or without clinical manifestation, in Africa and Europe. MATERIAL
Failure of antiviral chemotherapy: virus or cell resistance.: Int Conf AIDS. 1991 Jun 16-21;7(1):151 (abstract no. M.A.1236). Unique Identifier : AIDSLINE ICA7/1123691; Cinatl J; Cinatl J; Rabenau H; Geis H; Mainke M; Kornhuber B; Doerr HW; Centre of Hygiene, Department of Medical Virology, J.W. Goethe; University, Frankfurt a. Main; In the course of the last two years we have isolated several acyclovir-resistant mutants of HSV from patients with AIDS. Only recently, we obtained HSV type 1 isolates from two patients with AIDS and reccurent HSV type 1 infection, manifested by progression of mucocutaneous HSV lesions despite therapy with acyclovir.
Immune activation of latent HIV-1 expression in monocyte/macrophages.: Int Conf AIDS. 1991 Jun 16-21;7(1):151 (abstract no. M.A.1237). Unique Identifier : AIDSLINE ICA7/1123791; Mikovits JA; Lohrey N; Schuloff R; Ruscetti F; B CDP, PRI/Dyn Corp, NCI/FCRDC, Frederick, MD, USA; OBJECTIVE: To investigate the role of immunological stimuli in the activation of HIV expression from latently infected monocytes/macrophages from HIV infected patients. METHODS: A latently infected (no RNA expression) monocytoid cell line, THP-1, and freshly isolated adherent monocytes from asymptomatic seropositive i
Expression of human immunodeficiency virus type 1 in the cerebrospinal fluid detected by polymerase chain reaction (PCR) and its correlation with CNS disease.: Int Conf AIDS. 1991 Jun 16-21;7(1):151 (abstract no. M.A.1238). Unique Identifier : AIDSLINE ICA7/1123891; Goswami K; Tedder R; Hamel D; Harrison M; Daniels R; University College and Middlesex School of Medicine, London, UK; METHODS: The polymerase chain reaction (PCR) was used to amplify HIV-1 sequences in CSF and serum samples. Briefly, total RNA was extracted from these samples and reverse transcribed using either random primers or the antisense primer. The resulting cDNA was amplified in a first round PCR with outer primers. In nested
HIV-1 infection alters tyrosine phosphorylation in CD4+ T cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):151 (abstract no. M.A.1239). Unique Identifier : AIDSLINE ICA7/1123991; Cohen D; Tani Y; Welchlin-Bouadjemi H; Samelson LE; Lane HC; LIR, NIAID, NIH, Bethesda, MD USA; OBJECTIVE: Tyrosine phosphorylation of cellular substrates is a key event regulating T cell growth, and it is abnormal in an animal model (MRL lpr mouse) of dysfunctional T cell development. The mechanisms by which HIV-1 infection leads to CD4+ T cell depletion and paralysis of antigen specific helper cell proliferati
HIV-1 Mediated inhibition of enriched hematopoietic cell (CD34+) growth.: Int Conf AIDS. 1991 Jun 16-21;7(1):152 (abstract no. M.A.1240). Unique Identifier : AIDSLINE ICA7/1124091; Re Maria C; Zauli G; Visana G; Furlini G; Vignoli M; Gugliatta L; La Placa M; Institute of Microbiology, University of Bologna, Bologna, Italy; OBJECTIVE: In this work the effect of HIV-1 and envelope glycoprotein gp120 on the in vitro growth of enriched (CD34+) hematopoietic progenitors was investigated in liquid and semisolid cultures. METHODS: 25 bone marrow samples, obtained from consenting healthy donors, were enriched (up to 95-99%) in CD34+ cells. Enri
Quantification of HIV in peripheral blood: correlation with disease stage.: Int Conf AIDS. 1991 Jun 16-21;7(1):152 (abstract no. M.A.1241). Unique Identifier : AIDSLINE ICA7/1124191; O'Shea S; Rostron T; Singh NB; Banatvala JE; Virology Department, UMDS, St. Thomas' Campus, London, United; Kingdom; OBJECTIVE: Quantitation of HIV in peripheral blood mononuclear cells (PBMC) and correlation with clinical, virological and immunological status. METHODS: PBMC were obtained from symptomatic and asymptomatic patients and serial dilutions co-cultured with cord blood cells. Viral titres were expressed as a tissue culture
Using polymerase chain reaction to diagnose human herpes virus type 6 (HHV6) infection in HIV disease.: Int Conf AIDS. 1991 Jun 16-21;7(1):152 (abstract no. M.A.1242). Unique Identifier : AIDSLINE ICA7/1124291; Stroud S; Salvato P; Thompson C; Morrow J; Ragsdale D; Kotarba J; Houston Immunological Institute, Houston, Texas, USA; OBJECTIVE: To describe the role of polymerase chain reaction (PCR) in the diagnosis of HHV6 infection in HIV disease. METHODS: The sample consisted of 100 subjects from a population of 4,000 (50% HIV+) patients in an internal medicine practice. HIV+ (n=38), ARC (n=31) and AIDS (n=31) patients had DNA viral sequences (
Perinatal transmission of HIV-1: relationship between proviral copy number in vivo, viral properties in vitro and clinical outcome in infected children.: Int Conf AIDS. 1991 Jun 16-21;7(1):152 (abstract no. M.A.1243). Unique Identifier : AIDSLINE ICA7/1124391; Mammano F; De Rossi A; Pasti M; Ometto L; Giaquinto C; Chieco-Bianchi L; Institute of Oncology, University of Padova, Italy; OBJECTIVE: Study of HIV-1 copy number in PBMC in vivo, and in vitro cell tropism, replication rate and transactivation activity of HIV isolates from perinatally infected children, to correlate virologic properties with disease outcome. METHODS: HIV-1 copy number was determined by direct PCR in lysed patient cells usin
Biological and antigenic characteristics of sequential simian immunodeficiency virus isolates derived from experimentally inoculated cynomolgus macaque monkeys.: Int Conf AIDS. 1991 Jun 16-21;7(1):153 (abstract no. M.A.1244). Unique Identifier : AIDSLINE ICA7/1124491; Fenyo EM; Putkonen P; Albert J; Ohman P; Biberfeld G; Dept. of Virology, Karolinska Institute, Stockholm, Sweden; OBJECTIVE: To study the biologic and antigenic properties of simian immunodeficiency (SIV sm) isolates obtained throughout the entire course of infection, from day 12 postinfection until death from immunodeficiency. METHODS: Biologic characterization involved tests for replicative capacity of SIV sm isolates in human
Single step growth kinetics for HIV: induction of cell death by shutdown of DNA synthesis.: Int Conf AIDS. 1991 Jun 16-21;7(1):153 (abstract no. M.A.1245). Unique Identifier : AIDSLINE ICA7/1124591; Kiernan R; Doherty RR; McPhee D; Macfarlane Burnet Centre for Medical Research, Melbourne,; Victoria, Australia; OBJECTIVE: To achieve single step growth kinetic for HIV in order to understand the mechanism of cell killing. METHODS: HTLV-1 transformed MT-2 cells were infected at 100 pfu/cell with HIV-1 and equivalent inactivated virus, both derived from infection of MT-2 cells: Replication was assessed by RT activity, MT-4 plaqu
Cervical squamous intraepithelial lesions (SIL) in women with HIV: relationship to persistent human papillomavirus (HPV) infection of the cervix.: Int Conf AIDS. 1991 Jun 16-21;7(1):153 (abstract no. M.A.1246). Unique Identifier : AIDSLINE ICA7/1124691; Burk RD; Fleming I; Ho GY; Klein RS; Albert Einstein College of Med. Bronx, NY, USA; OBJECTIVE: Previous studies indicate that HIV immunosuppressed women are especially vulnerable to cervicovaginal infection with and neoplastic manifestations related to HPV. To understand the pathologic basis of these events, we have investigated the natural history of cervical HPV infection in a group of women at ris
HIV-1 isolates show a dissociation of reverse transcriptase (p66/p51) and core antigen (p24) production.: Int Conf AIDS. 1991 Jun 16-21;7(1):153 (abstract no. M.A.1247). Unique Identifier : AIDSLINE ICA7/1124791; Masquelier B; Delord B; Sallafranque-Andreola ML; Tarrago-Litvak L; Fleury HJ; Universite de Bordeuax II, Bordeaux, France; OBJECTIVE: To characterize the in vitro properties of two HIV-1 isolates from a patient receiving long-term treatment with zidovudine (ZDV). METHODS: Two HIV-1 isolates were obtained from the peripheral blood mononuclear cells of a patient treated for more than one year with ZDV. They were then grown in CEM cells (a T
HIV inhibitory factors in human saliva.: Int Conf AIDS. 1991 Jun 16-21;7(1):154 (abstract no. M.A.1248). Unique Identifier : AIDSLINE ICA7/1124891; Malamud D; Davis C; Roth E; Friedman H; Univ. of Pennsylvania, School of Dental Medicine and Medicine,; Phila. Pa USA; Previous reports have suggested that incubation of HIV in human whole or submandibular saliva inhibited infection of human lymphocytes. Our studies were designed to confirm and extend the findings of Fox et al to gain insight into the specificity and mechanism of the anti-HIV effect of saliva. Stimulated whole, paroti
IL-1 and IL-6 correct the accessory cell function deficiency of HIV-1 infected monocytes.: Int Conf AIDS. 1991 Jun 16-21;7(1):154 (abstract no. M.A.1249). Unique Identifier : AIDSLINE ICA7/1124991; Lacroix F; Fillion LG; Fan Z; Zhao D; Izaguirre C; University of Ottawa, Ottawa, Canada; OBJECTIVE: To determine the effect of HIV-1 infection on the accessory cell function of monocytes. METHODS: Accessory cell function was measured by the ability of monocytes to restore PHA and soluble anti-CD3 induced blastogenesis of highly purified and monocyte depleted (less than 0.5%) resting T-cells. Monocytes wer
Acute HIV-1 infection of peripheral blood lymphocytes is associated with enhanced integrin expression.: Int Conf AIDS. 1991 Jun 16-21;7(1):154 (abstract no. M.A.1250). Unique Identifier : AIDSLINE ICA7/1125091; Weeks BS; Klotman ME; Kleinman HK; Yamada KM; Klotman PE; Lab of Develop. Biology, NIDR, Bethesda, MD, USA; OBJECTIVE: Specific tissue targeting of virally infected cells or viral proteins may play a role in HIV-1 pathogenesis. Therefore, we examined the effects of viral infection on the expression of receptors involved in cell adhesion to basement membrane components. METHODS: PHA-stimulated peripheral blood lymphocytes (P
Pathogenesis of HIV-2 infection in macaque species.: Int Conf AIDS. 1991 Jun 16-21;7(1):154 (abstract no. M.A.1251). Unique Identifier : AIDSLINE ICA7/1125191; Morton WR; Schmidt AM; McCLure J; Gale M; Kuller L; Hu SL; Regional Primate Research Center, Seattle, WA, USA; HIV-2 has a high degree of homology to the simian immunodeficiency virus (SIV), shown to cause AIDS in several species of nonhuman primates. We have identified 2 subgroups of HIV-2 that have differing degrees of cytopathogenicity to macaque peripheral blood mononuclear cells (PBMCs) in vitro. In this study, we will co
Cytokines TNF-A and IL-1 stimulate HIV-1 proliferation from persistently infected human fetal brain cells in culture.: Int Conf AIDS. 1991 Jun 16-21;7(1):155 (abstract no. M.A.1252). Unique Identifier : AIDSLINE ICA7/1125291; Tornatore CS; Major EO; Section on Molecular Virology and Genetics, National Institute of; Neurological Disorders and Stroke, Bethesda, MD USA; OBJECTIVE: HIV-1 infection in the brain results in an encephalopathy which affects the function of neural and glial cells. To study the direct affect of HIV-1 on glial cells we have established a model of HIV-1 persistently infected human brain cells in culture. Experiments are described which identify several cytokin
Electron microscopic 3d modelling of HIV-induced syncytia and non infected controls.: Int Conf AIDS. 1991 Jun 16-21;7(1):155 (abstract no. M.A.1253). Unique Identifier : AIDSLINE ICA7/1125391; Bron C; Jendis J; Loeliger S; Bachi T; Schupbach J; Swiss National Center for Retroviruses, Institute for Immunology; and Virology, Zurich, Switzerland; OBJECTIVE: To develop techniques using single HIV-infected cells for directed immunocytochemical studies and 3D immunoelectron microscopic quantification. METHODS: Computer Aided Volumic Ultra Microscopy (CAVUM) takes advantages of correlative photon to electron microscopy and allows the 3D reconstruction and morphome
Generalized giant cell disease in SIV-infected cynomolgus monkeys.: Int Conf AIDS. 1991 Jun 16-21;7(1):155 (abstract no. M.A.1254). Unique Identifier : AIDSLINE ICA7/1125491; Su-Ling L; Kaaya EE; Putkonen P; Feichtinger H; Parravicini C; Ekman M; Biberfeld P; Immunopathology Lab., Karolinska Institute, Stockholm, Sweden; OBJECTIVE: To study the pathology of SIV infection in cynomolgus monkeys as a model of AIDS in man. METHODS: 28 monkeys were infected with simian immunodeficiency virus (SIVsmm3, from Dr. P. Fultz and McClure, Yerkes Primate Center, USA). Histopathological including immunohistochemical changes were studied in biopsies
Complement-enhanced HIV-1 binding to monocytes is dependent on alternative pathway activation, and mediated by complement receptors.: Int Conf AIDS. 1991 Jun 16-21;7(1):155 (abstract no. M.A.1255). Unique Identifier : AIDSLINE ICA7/1125591; Bakker LJ; Nottet HS; de Graaf L; de Vos NM; Van Strijp JA; Visser MR; Verhoef J; Eijkman-Winkler Laboratory for Medical Microbiology, University; of Utrecht, Utrecht, The Netherlands; OBJECTIVE and METHODS: Infection of mononuclear phagocytes with HIV-1 can be enhanced by complement, as has been shown by several investigators. In a previous report, we demonstrated that HIV-1 binding to human monocytes is increased by complement (Bakker et al, VI Int. AIDS Conf., F. A. 311). In the present study the
HIV infection of astroglial cell cultures from rat cerebral tissue.: Int Conf AIDS. 1991 Jun 16-21;7(1):156 (abstract no. M.A.1256). Unique Identifier : AIDSLINE ICA7/1125691; Romano N; Bonura F; Russo AD; Vitale F; Ferraro D; Dipartimento di Igiene a Microbiologia, University of Palermo,; Italy; OBJECTIVE: The possibility that primary cultures of astrocytes of rats might be a suitable experimental model to study HIV replication in cells of glial origin was assessed. METHODS: Mixed glial cell cultures were prepared from dissociating cerebral cortex of 1 to 2-d-old rat pups. The astrocytes were separated from t
Biological characteristics of HIV-1 isolates from blood and cerebrospinal fluid of AIDS patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):156 (abstract no. M.A.1257). Unique Identifier : AIDSLINE ICA7/1125791; Russo Alesi D; Bonura F; Vitale F; Romano N; Farinelli V; Bruno M; Dipartimento di Igiene e Microbiologia, University of Palermo,; Palermo, Italy; OBJECTIVE: The possibility that isolates of HIV-1 from the brain may constitute a special group of AIDS virus was assessed. METHODS: Paired cerebrospinal fluid (CSF) and peripheral blood (PB) HIV-1 strains were isolated from 10 AIDS patients in human blood mononuclear cells (HBMC). Primary monocyte cultures, monocyte-
HIV induces both activation and suppression of T lymphocytes: induction of phosphorylation of tyrosine residues and lymphokine gene expression as well as suppression of proliferation.: Int Conf AIDS. 1991 Jun 16-21;7(1):156 (abstract no. M.A.1258). Unique Identifier : AIDSLINE ICA7/1125891; Hofmann B; Nishanian P; Nguyen T; Insixiengmay P; Fahey JL; UCLA School of Medicine, Los Angeles. CA 900424-1747; OBJECTIVE: We have earlier shown that HIV proteins induce a partial suppression of the early steps of lymphocyte activation including inositol phospholipid metabolism leading to reduced cell proliferation (J. Immunol. Hofmann et al 1990). The aim of this study was to further identify steps of lymphocyte activation sup
Fusion of HIV-1 & HIV-2 infected monocyte/macrophage (Mphi) with uninfected CD4-bearing T-lymphoblastoid cells: a transmission of electron microscopic examination.: Int Conf AIDS. 1991 Jun 16-21;7(1):156 (abstract no. M.A.1259). Unique Identifier : AIDSLINE ICA7/1125991; Boothman J; Marshall J; Colvin A; Sonza S; Crowe SM; Macfarlane Burnet Centre for Medical Research and Virology,; Fairfield Hospital, Melbourne, Australia; OBJECTIVE: To define at an electron microscopic level the cell fusion process between HIV-1 and HIV-2 infected mphi with uninfected CD4-bearing T lymphoblastoid cells (VB). METHODS: Mphi were isolated from HIV-seronegative donors via density centrifugation and glass adherence and cultured in suspension in RPMI-1640/10
RNA-protein complexes interfere with inhibition of reverse transcriptase.: Int Conf AIDS. 1991 Jun 16-21;7(1):157 (abstract no. M.A.1260). Unique Identifier : AIDSLINE ICA7/1126091; Lightfoote MM; Bailey JM; Food and Drug Administration, Center for Devices and Radiological; Health; OBJECTIVE: To determine the importance of cellular factors on HIV reverse transcriptase activity, and to determine if these factors vary among cell types, influencing the efficiency of the viral enzyme. METHODS: RNA-protein complexes isolated from infected or uninfected cells were identified on agarose gels. Further a
Retroviral effects on muscle cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):157 (abstract no. M.A.1261). Unique Identifier : AIDSLINE ICA7/1126191; Trujillo JR; Gomez-Lucia E; Lee TH; Essex M; Harvard University School of Public Health, Boston, MA, USA; OBJECTIVE: Whether or not retroviruses play a direct or indirect role in myopathy is unclear. The objective of this work is to compare and study in vitro the role of HIV-1 and HTLV-1 infection on muscle cells. METHODS: We used 5 strains of HIV-1 and 5 strains of HTLV-1 for infection of two muscle cell lines (A-204, HS
Release of HIV-1 core protein from infected cells independent of infectious virus particles.: Int Conf AIDS. 1991 Jun 16-21;7(1):157 (abstract no. M.A.1262). Unique Identifier : AIDSLINE ICA7/1126291; Grunow R; Valentin A; Fenyo EM; Wahren B; Jondal M; Karolinska Institute, Stockholm, Sweden; OBJECTIVE: In the serum of most HIV infected persons a significant increase of viral p24 core protein is detectable with the development of the AIDS. The aim was to study whether the p24 is the result of degradation of virus particles or infected cells, or whether p24 could be released as non-virion proteins from livi
Heterogeneity in the oligosaccharide structures of the envelope glycoprotein (gp120) of HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):157 (abstract no. M.A.1263). Unique Identifier : AIDSLINE ICA7/1126391; Pal R; di Marzo Veronese F; Nair B; Sarngadharan M; Advanced BioScience Laboratories, Inc., Kensington, Maryland, USA; OBJECTIVE: To study the heterogeneity in the N-linked oligosaccharide structures of gp120 synthesized in chronically infected Molt3 cells. METHODS: Metabolically labeled HIV-1 glycoprotein was immunoprecipitated from chronically infected cells and the immunoprecipitates were digested with endo H to probe mannose-rich
Protection from viremia and disease progression in SIVBK28 infected Macaca mulatta, challenged with SIVmac251/32H: Int Conf AIDS. 1991 Jun 16-21;7(1):158 (abstract no. M.A.1264). Unique Identifier : AIDSLINE ICA7/1126491; Niphuis H; Heeney JL; ITRI-TNO, Dept. of Chronic and Infectious Diseases, Rijswijk, The; Netherlands; Various strains of the HIV-2 related SIVsm group of primate lentiviruses have different pathogenic properties in Macaca mulatta. SIVBK28 is a relatively non-pathogenic and non-viremic strain causing persistent infection with a strong persistent immune response. OBJECTIVE: To determine if prior infection with a minimal
Disease progression correlates with selective loss of CD4+/CD29+ memory T-cells in SIV-infected rhesus macaques, HIV-infected individuals, but not in HIV-infected chimpanzees.: Int Conf AIDS. 1991 Jun 16-21;7(1):158 (abstract no. M.A.1265). Unique Identifier : AIDSLINE ICA7/1126591; Heeney JL; Miedema F; Niphuis H; ITRI-TNO, Dept. of Chronic and Infectious Diseases, Rijswijk, The; Netherlands; Simian immunodeficiency virus (SIV) induces an AIDS like syndrome in rhesus macaques similar to HIV in man. Immunological abnormalities can be demonstrated in macaques infected with SIV similar to man infected with HIV. Chimpanzees infected with HIV-1 show no signs of immunosuppressive disease development to date. In
Definition of an HIV p24 epitope endowed with distinctive immunosuppressive properties.: Int Conf AIDS. 1991 Jun 16-21;7(1):158 (abstract no. M.A.1266). Unique Identifier : AIDSLINE ICA7/1126691; Pugliese O; Luzzati AL; Giacomini E; Giordani L; Camponeschi B; Chersi A; Evangelista M; Dept. of Immunology, Ist. Superiore de Sanita, Rome, Italy; OBJECTIVE: We have previously shown that a 20 aminoacids peptide (aa 218-237) of p24 is able to block antigen-specific responses in vitro. Objective of the present study is a better definition of the responsible epitope. METHODS: Peptides, corresponding to progressively shorter sequences in the 218-238 region of p24,
Soluble CD4 inhibits HIV gp120-induced impairment of signal transduction in human CD8+ T-cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):158 (abstract no. M.A.1267). Unique Identifier : AIDSLINE ICA7/1126791; Yoshitatsu S; Prince AK; Laboratory of Neuroscience, NIDDK, National Institutes of Health,; Bethesda, MD, USA; OBJECTIVE: Impaired transmembrane signalling by HIV proteins may contribute substantially to the alterations in immune responsiveness in HIV-infected individuals. To investigate the mechanisms responsible for immune suppression produced by viral products, the effects of HIV-gp 120 on signal transduction in a variety o
Stimulation of proliferation rate during HIV infection in CD4 positive cell lines.: Int Conf AIDS. 1991 Jun 16-21;7(1):159 (abstract no. M.A.1268). Unique Identifier : AIDSLINE ICA7/1126891; Di Rienzo AM; Petronini PG; Guetard D; Favilla R; Borghetti AF; Montagnier L; Piedimonte G; Unite d'Oncologie Virale, Institut Pasteur, Paris, France; OBJECTIVE: We have observed that the mitotic index of CD4+ cell lines (CEM and U937) is significantly increased when the cells are infected by purified HIV preparations suggesting that the virus alone could be endowed with mitogenic activity. This presumptive virus-induced mitogenic activity and its possible involveme
Modulation of protein synthesis rate during HIV infection in a CD4-positive cell line.: Int Conf AIDS. 1991 Jun 16-21;7(1):159 (abstract no. M.A.1269). Unique Identifier : AIDSLINE ICA7/1126991; Petronini PG; Di Rienzo AM; Urbani S; Guetard D; Borghetti AF; Piedimonte G; Instituto di Patologia Generale, Universita di Parma, Parma,; Italy; OBJECTIVE: The pathogenetic mechanism of lytic viruses usually involves a large domination of host protein synthesis. In contrast, during the life cycle of a typical retrovirus only little interference with the cell biosynthetic machinery occurs. It has been suggested that the retrovirus HIV takes over the cellular ma
Envelope shedding and high cell density: barriers to vaccine and immunotherapy development.: Int Conf AIDS. 1991 Jun 16-21;7(1):159 (abstract no. M.A.1270). Unique Identifier : AIDSLINE ICA7/1127091; Layne S; Merges M; Dembo M; Spouge J; Nara P; Los Alamos National Laboratory, Los Alamos, NM, USA; OBJECTIVE: HIV-1 and -2 share two properties that are important for understanding immunity and therapy. First, HIV preferentially infects CD4+ cells that have a wide range of densities in lymphoid, reticuloendothelial and nervous tissue. Second, the density of gp120 knobs covering the surface of HIV decreases with tim
Experimental and theoretical indications for HIV-1 nef neurotoxicity based on its similarity to scorpion neurotoxines.: Int Conf AIDS. 1991 Jun 16-21;7(1):159 (abstract no. M.A.1271). Unique Identifier : AIDSLINE ICA7/1127191; Brack-Werner R; Werner T; Mager R; Saermark T; Banati RB; Ferroni S; Steinaa L; Kreutzberg GW; Erfle V; GSF-Forschungszentrum fur Umwelt und Gesundheit GmbH; Ingolstadter LandstraBe 1, D-8042 Neuherberg; OBJECTIVE: Neuropatholoical analysis of HIV-1 infected brains suggests contribution of general toxic processes. We investigated potential involvement of HIV-1 nef protein in these processes. Next to HIV structural proteins and the regulatory proteins tat and vif, human glial cells persistently infected with HIV-1 show
Intracellular localization of HIV-1 nef in permanently infected glial cells.: Int Conf AIDS. 1991 Jun 16-21;7(1):160 (abstract no. M.A.1272). Unique Identifier : AIDSLINE ICA7/1127291; Kohleisen B; Herrmann R; Krohn K; Erfle V; GSF Abt.Molekulare Zellpathologie, Neuherberg, FRG; OBJECTIVE: Human glioblastoma cells permanently infected with HIV-1 (TH 4-7-5) serve as a model system for virus latency characterized by a high expression of the viral regulatory protein nef. Detailed studies are performed concerning the localization of nef in different cellular compartments of TH 4-7-5 cells. METHOD
Pathogenicity of HIV-1 in the absence of envelope protein expression.: Int Conf AIDS. 1991 Jun 16-21;7(1):160 (abstract no. M.A.1273). Unique Identifier : AIDSLINE ICA7/1127391; Gurgo C; Gallo RC; C.E.O.S.-CNR, Naples, Italy; OBJECTIVE: To look for cytopathic effects of HIV-1 independent of env gene expression. METHODS: The initiation codon of the envelope (e) gene of HXB2 and a second initiation codon in frame with the first were mutated into stop codons by primer directed mutagenesis, and the premature stop codon in the nef (n) gene was
Immunohistochemical localization of laminin and type IV collagen in HIV encephalitis.: Int Conf AIDS. 1991 Jun 16-21;7(1):160 (abstract no. M.A.1274). Unique Identifier : AIDSLINE ICA7/1127491; Cenacchi G; Giangaspero F; Martinelli GN; Istituto di Microscopia Elettronica Clinica, Italy; Infection by HIV of the brain causes diffuse degeneration of cerebral and cerebellar white matter. The lesions are most marked in the centrum semiovale, while subcortical white matter is usually spared. The pathogenesis of these alterations is still open to discussion. Increased permeability of the blood-brain-barrier
Virus load in CD4+ T cells in asymptomatic HIV-1-infected individuals.: Int Conf AIDS. 1991 Jun 16-21;7(1):160 (abstract no. M.A.1275). Unique Identifier : AIDSLINE ICA7/1127591; Brinchmann JE; Albert J; Vartdal F; Institute of Transplantation Immunology, The National Hospital,; Oslo, Norway; OBJECTIVE: To determine the proportion of CD4+ T cells containing HIV-1 provirus, the number of provirus copies contained in each infected cell and the replication competence of the provirus copies in CD4+ T cells in asymptomatic HIV-1 infected individuals. METHODS: Blood from six immunologically and clinically stable
Human blood monocytes from HIV-1 seropositive patients are usually not infected with HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):161 (abstract no. M.A.1276). Unique Identifier : AIDSLINE ICA7/1127691; Izaguirre C; Shen YN; Cassol S; Rudknik J; Drouin J; Cameron W; Federal Centre for AIDS, Health and Welfare Canada; OBJECTIVE: In HIV patients tissue macrophages are infected but it is uncertain whether blood monocytes are infected. We wished to determine if monocytes from infected patients are HIV positive or not. METHODS: Cell subsets were separated using monoclonal antibodies linked to magnetic beads. Monocytes adherence to glas
Presence of extrachromosomal HIV-1 DNA in T cell clones from PBL of an HIV-1 seropositive asymptomatic subject.: Int Conf AIDS. 1991 Jun 16-21;7(1):161 (abstract no. M.A.1277). Unique Identifier : AIDSLINE ICA7/1127791; Borsetti A; Titti F; Testa U; Care A; Federico M; Sernicola L; Samoggia P; Verani P; Peschle C; Rossi GB; Dept. of Virology, Istituto Superiore di Sanita, Rome, Italy; OBJECTIVE: To examine if the accumulation of unintegrated viral DNA might explain the ability of HIV-1 to establish and maintain latent or chronic infection in peripheral blood cells of HIV-1 seropositive subjects. METHODS: PBL from an asymptomatic HIV-1 seropositive and HTLV-I seronegative (ELISA, WB) subject were cl
Maternal-fetal transmission of HIV: permissivity of primary cultures of human term trophoblast to the virus.: Int Conf AIDS. 1991 Jun 16-21;7(1):161 (abstract no. M.A.1278). Unique Identifier : AIDSLINE ICA7/1127891; Zachar V; Ebbesen P; Mayer V; Institute of Virology, Slovak Acad. Sci., Bratislava,; Czechoslovakia; OBJECTIVE: To examine the ability of trophoblast cells/TC/ to support replication of the HIV-1 using strains with diverse cytopathogenicity and to express complete proviral clones after transfection. METHODS: The primary cultures of trypainized TC/Percoll, magnetic immunnoaffinity purification, Mab identification, vir
Productive infection of human colon cancer cells with HIV-1 induces an intense activity of secretion.: Int Conf AIDS. 1991 Jun 16-21;7(1):161 (abstract no. M.A.1279). Unique Identifier : AIDSLINE ICA7/1127991; Fantini J; Yahi N; Baghdiguian S; Chermann JC; Inserm Unite 322, Marseille, France; OBJECTIVES: To demonstrate an impairment of the secretory function in colon carcinoma cells infected with HIV. METHODS: HT29 cells were infected with 2 strains of HIV1 (BRU and NDK) and 1 strain of HIV2 (ROD). Viral production was monitored after co-cultivation with CEM cells or directly in the culture supernatant by
Natural progression of simian immunodeficiency virus (SIVagm) infection in wild-caught South African vervet monkeys.: Int Conf AIDS. 1991 Jun 16-21;7(1):162 (abstract no. M.A.1280). Unique Identifier : AIDSLINE ICA7/1128091; Smit TK; Schoub BD; Lyons SF; MRC AIDS Virus Research Unit, National Institute for Virology,; Sandringham, South Africa; OBJECTIVE: SIVagm seroprevalence in wild-caught South African vervet monkeys (Cercopithecus aethiops) was determined in serial, stored and fresh serum samples from 1983 to 1990 and 12 isolates were compared antigenically. METHODS: Sera were examined for antibodies to SIVagm by an indirect immunofluorescence assay. SIV
A proposed mechanism for activation of HIV infection by mycoplasma organisms persisting in hematopoietic tissues.: Int Conf AIDS. 1991 Jun 16-21;7(1):162 (abstract no. M.A.1281). Unique Identifier : AIDSLINE ICA7/1128191; Sanin AV; Sosnovskaya O; The Gamaleya Institute, Moscow, USSR; Many microorganisms are able to persist in the bone marrow for a very long time. In AIDS patients serious damage of hematopoiesis is found. A troubling stimulatory role of mycoplasma in AIDS has been recently revealed (Montagniex, 1990). We aimed to develop an approach to identify target cells for Mycoplasma arthritid
The use of novel methodology for the determination of the prevalence of HTLV-I and HTLV-II infections in an HIV-I positive intravenous drug user cohort in Dublin.: Int Conf AIDS. 1991 Jun 16-21;7(1):162 (abstract no. M.A.1282). Unique Identifier : AIDSLINE ICA7/1128291; Tuke PW; Garson JA; Loveday C; Mulcahy F; Tedder RS; Division of Virology, University College and Middlesex School of; Medicine, London, UK; OBJECTIVE: To develop a specific and sensitive method for the detection and typing of HTLV infections, which is applicable to cryopreserved whole blood, and use this to determine the prevalence of HTLV infections in a cohort of HIV-I infected intravenous drug users. METHOD: Whole blood samples from a cohort of 70 HIV-
Antibodies to structural and nonstructural polypeptides of D-type retrovirus in patients with AIDS.: Int Conf AIDS. 1991 Jun 16-21;7(1):162 (abstract no. M.A.1283). Unique Identifier : AIDSLINE ICA7/1128391; Ilyin KV; Morozov VA; Ilyinski PO; Kzhyshkowska JG; Cherenovsky DN; Miller GG; Cancer Research Center AMS USSR; The structural and nonstructural polypeptides of D-type retroviruses (HEp-2V and MPMV) we used as antigens in radioimmunoprecipitation (RIP) and in Western blotting. A polypeptide with molecular weight 78 Kd has been specifically precipitated with goat anti-p27 serum. This protein was shown to be gag gene-coded protei
Isolation and characterization of HTLV-II from Italian IVDUs.: Int Conf AIDS. 1991 Jun 16-21;7(1):163 (abstract no. M.A.1284). Unique Identifier : AIDSLINE ICA7/1128491; Bertazzoni U; Zella D; Mori L; Cavicchini A; Sala M; Casoli C; Magnani G; Ferrante P; Lori F; Landini V; et al; IGBE/CNR, Pavia; OBJECTIVE: Identification of HTLV-I and HTLV-II among subjects of risk for AIDS. Characterization of new HTLV-II viral isolates and study of genomic variability. METHODS: Patients: 678 IVDUs (80% HIV-1 positive) screened for HTLV-I and HTLV-II with PPA, WB and IFA assays. PBMCs from 19 of 47 positive cases further ana
Viral infections in AIDS and in immunocompetent patients suffering from non-Hodgkin's lymphoma (NHL).: Int Conf AIDS. 1991 Jun 16-21;7(1):163 (abstract no. M.A.1285). Unique Identifier : AIDSLINE ICA7/1128591; Lourenco MH; Azovedo Pereira JM; Santos Ferreira MO; Forjaz de Lacerda MJ; Forjaz de Lacerda J; Faculty of Pharmacy, University of Lisbon, Portugal; OBJECTIVE: To compare the prevalence of viral infections in AIDS and in immunocompetent patients suffering from NHL. METHODS: 182 patients were investigated for virological infections. The serological tests included: hepatitis B surface antigen (HBs Ag) and antibody (HBs Ab), hepatitis B core antibody (HBc Ab), Herpes
HTLV-I virus DNA amplification in French blood donors.: Int Conf AIDS. 1991 Jun 16-21;7(1):163 (abstract no. M.A.1286). Unique Identifier : AIDSLINE ICA7/1128691; Wattel E; Mariotti M; Agis F; Gordien E; Ferrer-le-Coeur F; Elghouzzi MH; Lefrere JJ; Fondation Nationale de Transfusion Sanguine, Paris, France; OBJECTIVE: To affirm the presence of HTLV-I DNA through polymerase chain reaction (PCR) assay in French blood donors with indeterminate Western blot (WB) assay. METHODS: 38 blood donors repeatably reactive in HTLV-I/II ELISA (Abbott) detected in the French West Indies (n = 25) or in the Paris area (n = 13) and 20 sero
HIV-2 gp36 monoclonals which distinguish HIV-2 isolates from each other and from SIV and HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):163 (abstract no. M.A.1287). Unique Identifier : AIDSLINE ICA7/1128791; Walther L; Biberfeld G; Thorstensson R; Dept. of Immunology, National Bact. Lab., Stockholm, Sweden; OBJECTIVE: To study the reactivity 1) of commercial anti HIV-2 Western blots and 2) of different HIV-1 and HIV-2 and SIV isolates by use of three monoclonals against HIV-2 gp36. METHODS: Monoclonals were produced in mice immunized with purified and whole viral lysate of HIV-2 (SBL-6669). HIV-1, HIV-2 Western blot stri
HTLV-1/2 co-infection among 3133 HIV+ intravenous drug abusers from Lombardy.: Int Conf AIDS. 1991 Jun 16-21;7(1):164 (abstract no. M.A.1288). Unique Identifier : AIDSLINE ICA7/1128891; Rescaldani R; Salvaggio L; Goglio A; Malacrida V; Mocarelli P; Ferrante P; Visconti A; AMCLI Study Group on AIDS, Milano, Italy; OBJECTIVE: To evaluate the prevalence of HTLV-1/2 co-infection among HIV+ intravenous drug abusers (IVDAs) from Lombardy, the region with the highest incidence of AIDS in Italy . METHODS: We have screened 3133 HIV+ IVDAs attending the Drug Dependence Units of 6 regional general hospi
HTLV-I and HTLV-II in Spain.: Int Conf AIDS. 1991 Jun 16-21;7(1):164 (abstract no. M.A.1289). Unique Identifier : AIDSLINE ICA7/1128991; Soriano V; Leon-Monzon M; Tor J; Pujci M; Sabarre E; Granada I; Babria M; Hospital Germans Trias i Pujol, Barcelona, Spain; OBJECTIVES: HTLV-I is the etiological agent of ATL and TSP/HAM. The virus has also been related to other diseases such as lymphoproliferative disorders other than ATL, Multiple Sclerosis, Sjogren syndrome, Systemic lupus arythematous, polymyositis and infective dermatitis. To reduce the risk of infection by blood tran
Serum reactivity against HIV-1, HTLV-I and HTLV-II antibodies in rheumatic diseases.: Int Conf AIDS. 1991 Jun 16-21;7(1):164 (abstract no. M.A.1290). Unique Identifier : AIDSLINE ICA7/1129091; Soriano V; Leon-Monzon M; Ordi J; Font J; Porcel M; Escudero D; Tor J; Hospital Germans Trias i Pujol, Barcelona, Spain; OBJECTIVES: Suggestions on a possible retroviral etiology for rheumatic diseases has been recently reported. Serologic reactivity against specific proteins of both HIV-1 and HTLV-I has been recognised in serum of patients with Polymyositis (PM), Systemic lupus erythematous (SLE), Sjogren syndrome (SS) and Reiter syndr
HIV-2 in Spain.: Int Conf AIDS. 1991 Jun 16-21;7(1):164 (abstract no. M.A.1291). Unique Identifier : AIDSLINE ICA7/1129191; Soriano V; Granada I; Leal M; Valls F; Rivero A; Fernandez GL; Aguado I; Tor J; Condom MJ; Balanzo P; Hospital Germans Trias i Pujol, Barcelona, Spain; OBJECTIVES: HIV-2 is a second AIDS virus that was isolated in 1986 from West African individuals. Since that moment, HIV-2 infection has been described in several regions other than West Africa, especially in Europe. In Portugal , mo
HTLV-I and HTLV-II infections in the Friuli Venezia-Giulia region.: Int Conf AIDS. 1991 Jun 16-21;7(1):165 (abstract no. M.A.1292). Unique Identifier : AIDSLINE ICA7/1129291; D'Agaro P; Comar M; Dal Molin M; Faruzzo A; Moise G; Majori L; Giacca M; Roscioli B; Mascioli M; Ceccherini-Nelli L; et al; Inst. of Hygiene; Pisa, Italy; OBJECTIVE: To evaluate the prevalence of HTLV-I and HTLV-II among the groups at risk of the Friuli Venezia-Giulia region. METHODS: 310 drug addicts (IVDU), 60 subjects with possible sexual exposure (PSE) and 140 Hemodialysis patients were examined. Sera reactive at HTLV-I ELISA were submitted to HTLV-I Western blot (W
Immunoreactivity of Italian and West African sera for HTLV-1 and HTLV-2 antigens.: Int Conf AIDS. 1991 Jun 16-21;7(1):165 (abstract no. M.A.1293). Unique Identifier : AIDSLINE ICA7/1129391; Reina S; Narvanen A; Sabbatani S; Ferro A; Chan L; Lillo F; Varnier OE; Lab of Human Retrovirology, Genova, Italy; OBJECTIVE: Identification of type specificity in Italian and West African sera for HTLV-1 and HTLV-2 antigens. METHODS: Samples collected from 1,000 Italian subjects at risk for retroviral infections and 1,200 blood donors from Guinea Bissau were tested with reagents based on viral
Prevalence of anti HTLV-I antibodies in HIV seropositives in Guadalajara, Mexico.: Int Conf AIDS. 1991 Jun 16-21;7(1):165 (abstract no. M.A.1294). Unique Identifier : AIDSLINE ICA7/1129491; Campos-Lopez P; Vazquez-Valls E; Torres-Mendoza BM; Tlacuilo-Parra JE; Perez-Rios AM; Grupo de Diagnostico, Tratamiento e Investigacion Biomedica de; Guadalajara, A.C.; There are no publications about the prevalence of anti HTLV-I antibodies in the population of Mexico with or without association to HIV infection. OBJECTIVE: To demonstrate the presence of anti HTLV-I antibodies in HIV seropositives. METHODS: From 1,411 ELISA positive HIV sera, con
Isolation of SIV from 2 wild-captured mangabeys in Cote-Ivoire: Characterization and relatedness to HIV-2 strains from the same region.: Int Conf AIDS. 1991 Jun 16-21;7(1):165 (abstract no. M.A.1295). Unique Identifier : AIDSLINE ICA7/1129591; Fransen K; Gershy-Damet GM; Peeters M; Louwagie J; Nys P; Piot P; van der Groen G; Institute of Tropical Medicine, Antwerp, Belgium; OBJECTIVES: To investigate the presence of HIV-2 like viruses in different species of wild-captured monkeys from Cote-Ivoire, and to study their relatedness with other HIV-2 isolates. METHODS: Sera from 44 wild-captured monkeys (chimpanzees, baboons, African green monkeys and mangabeys) were tested with commercial HIV
Immunological and molecular characterization of two immunodeficiency virus isolates from wild captured chimpanzees.: Int Conf AIDS. 1991 Jun 16-21;7(1):166 (abstract no. M.A.1296). Unique Identifier : AIDSLINE ICA7/1129691; Peeters M; Fransen K; Van den Haesevelde M; Gershy-Damet GM; Temmerman M; Delaporte E; Hoofd G; Saman E; Piot P; van der Groen G; Institute of Tropical Medicine, Antwerp, Belgium; OBJECTIVES: To further characterize SIVcpz-gab and SIVcpz-ant isolates; to study their immunologic relatedness; to compare their sequence homology. METHODS: Isolates were characterized by Western blot and RIPA. HIV-1 antibody positive sera (n=257) from different geographic regions (Europe n=40), Central Africa (
Seroepidemiology of HTLV1/2 in blood donors and in haematological patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):166 (abstract no. M.A.1297). Unique Identifier : AIDSLINE ICA7/1129791; Granati L; Mannella E; Piro L; Pezzella M; Filoni F; Miceli M; Isacchi G; University of Rome La Sapienza, Rome, Italy; OBJECTIVE: To evaluate the prevalence of HTLV1/2 among haematological patients and blood donors (B.D.). METHODS: 37.423 who meet criteria for blood donation and 2,500 patients affected by haematological disorders were tested for HTLV1/2 antibodies by ETA. (Dupont, Abbott). All repeatedly reactive samples were confirme
Is an HTLV-like retrovirus endemic in Southern Apulia (Italy)?: Int Conf AIDS. 1991 Jun 16-21;7(1):166 (abstract no. M.A.1298). Unique Identifier : AIDSLINE ICA7/1129891; Tagliaferro L; Corbelli M; Boeri E; Franchini G; Huntala ML; Narvanen A; Ceo Y; Reina S; Lillo F; Varnier OE; Lab. Pignatelli, Lecce, Italy; OBJECTIVE: The aim of this study is to verify the presence of an HTLV-like retrovirus in Southern Apulia ( Italy ). METHODS: More than 2,000 samples collected from blood donors and normal individuals living in this valley were screened using a high sensitive agglutination test. ELISAs
Prevalence of HTLV infection type I and II in Gabon: comparison of the serological and PCR results.: Int Conf AIDS. 1991 Jun 16-21;7(1):166 (abstract no. M.A.1299). Unique Identifier : AIDSLINE ICA7/1129991; Delaporte E; Monplaisir N; Louwagie J; Peeters M; Larouze B; d'Auriol L; Louis JP; Trebucq A; Van Heuverswyn H; Piot P; et al; Institute of Tropical Medicine, Antwerp Belgium; OBJECTIVES: 1) To determine the HTLV-I and HTLV-II prevalence in Libreville, Gabon . 2) To compare the detection of HTLV-I and II by PCR with detection of HTLV-I/II antibodies. METHODS: A total of 322 asymptomatic adults were selected by the cluster sampling method. Sera were tested
Co-infection with HIV-1 and HTLV: Effect on lymphocyte phenotype.: Int Conf AIDS. 1991 Jun 16-21;7(1):167 (abstract no. M.A.1300). Unique Identifier : AIDSLINE ICA7/1130091; Fletcher MA; OBJECTIVE: To determine co-infection effect of HTLV and HIV-1 on lymphocyte subsets. METHODS: In a multicenter study, blood donors and subjects transfused with blood components were tested for anti-HIV-1 and anti-HTLV. For each dually infected person, three groups of 3 controls were selected: anti-HIV-1 (-)/anti-HTLV
Mapping the type-specific epitopes in the HTLV-II envelope glycoprotein.: Int Conf AIDS. 1991 Jun 16-21;7(1):167 (abstract no. M.A.1301). Unique Identifier : AIDSLINE ICA7/1130191; Chen YM; Samuel K; Blattner W; Essex M; Papas T; Laboratory of Molecular Oncology, National Cancer Institute,; Frederick, MD; OBJECTIVE: Previous report (Lancet 336: 1153-1155, 1990) showed recombinant protein IIB which has 140 amino acids from HTLV-II envelope protein and contains both type-specific and generic epitopes. To further map the type-II specific epitope in RP-IIB, we conducted this study. METHODS: A PCR-amplified DNA fragment enc
Biological characterization of HTLV-II fusion.: Int Conf AIDS. 1991 Jun 16-21;7(1):167 (abstract no. M.A.1302). Unique Identifier : AIDSLINE ICA7/1130291; Ugen K; Hall W; Sato A; Williams W; Levy D; Weiner D; The Wistar Institute, Philadelphia, PA; BACKGROUND AND OBJECTIVE: Human T cell leukemia virus II has been associated with benign hairy T cell leukemia although a strong disease association has not been substantiated. Recently, HTLV-II has been associated with chronic fatigue syndrome. A significant percentage of HIV-1 infected intravenous drug users in the
Monoclonal antibodies against external envelope protein (gp120) of HIV-2 rod.: Int Conf AIDS. 1991 Jun 16-21;7(1):167 (abstract no. M.A.1303). Unique Identifier : AIDSLINE ICA7/1130391; Matsushita S; Matsumi S; Murakami T; Maeda Y; Hattori T; Takatsuki K; Kumamoto University Medical School, Kumamoto, Japan; OBJECTIVE: The purpose of these studies was to raise the monoclonal antibodies that neutralize HIV-2 infection and to identify a neutralizing epitope of HIV-2. METHODS: Two peptides (p1, p2) corresponding to the parts of amino acid sequence derived from the external envelope protein (gp120) of HIV-2 ROD were synthesiz
Synthetic peptides in HIV-1, HIV-2 and HTLV I serology.: Int Conf AIDS. 1991 Jun 16-21;7(1):168 (abstract no. M.A.1304). Unique Identifier : AIDSLINE ICA7/1130491; Narvanen A; Korkolainen M; Kontio S; Lillo F; Varnier O; Rescaldani R; Suni J; Vaheri A; Huhtala ML; Labsystems Research Laboratories, Helsinki, Finland; OBJECTIVE: To determine the serological sensitivity and type-specificity of the synthetic peptides derived from HIV-1, HIV-2 and HTLV I structural proteins using epitope scanning techniques and indirect EIA. METHODS: The structural proteins of HIV-1, HIV-2 and HTLV I were scanned using a 14 amino acid window moved in
A 10-year prevalence study of infection with simian immunodeficiency virus and simian T-cell leukemia virus in a semi-free ranging mandrill colony.: Int Conf AIDS. 1991 Jun 16-21;7(1):168 (abstract no. M.A.1305). Unique Identifier : AIDSLINE ICA7/1130591; Yang R; Muloko-Ntoutoume N; Dixson A; Ossari S; Bedjabaga I; International Centre for Medical Research, Franceville, Gabon; OBJECTIVE: To evaluate seroprevalence of antibodies to natural infection with SIVmnd and STLV-1 among mandrills living in a semi-free tropical rainforest enclosure. METHODS: A breeding colony of 14 mandrills was established in 1980 at the CIRMF Primate Centre. In 1983 the animals were released into a 7ha rainforest en
Prevalence of anti-HTLV-I among Italian IVDAs and hemophiliacs.: Int Conf AIDS. 1991 Jun 16-21;7(1):168 (abstract no. M.A.1306). Unique Identifier : AIDSLINE ICA7/1130691; Zebender G; Galli C; Capelli C; Baloechini E; Orso M; Boschini A; Mari D; Cargnel A; Zanetti AR; Institute of Virology, University of Milan, Italy; The seroprevalence of antibodies to Human T-Lymphotropic Virus type I (HTLV-I) was evaluated in 1247 subjects belonging to groups at high risk for HIV infection. The percentage of HTLV-I seropositives among intravenous drug abusers was 4.4%, while no serological evidence of HTLV-I infection was found in hemophiliacs,
Multiple transcripts encode the trans-activator of EIAV.: Int Conf AIDS. 1991 Jun 16-21;7(1):168 (abstract no. M.A.1307). Unique Identifier : AIDSLINE ICA7/1130791; Noiman S; Yaniv A; Tsach T; Miki T; Tronick S; Gazit A; University of Tel Aviv, Tel Aviv, Israel; Equine infectious anemia virus (EIAV) induces a disease of horses most often characterized by recurring symptoms of fever, anemia, glomerulonephritis and uremia. Infections can be categorized into three distinct clinical phases: acute disease associated with initial exposure to virus, subacute (or chronic) disease, an
Analysis of indeterminate human T-lymphotropic virus type I (HTLV-I) Western blot interpretations reported for HTLV-type II infected individuals.: Int Conf AIDS. 1991 Jun 16-21;7(1):169 (abstract no. M.A.1308). Unique Identifier : AIDSLINE ICA7/1130891; Blumer SO; Schalla WO; Cross GD; Hancock JS; Muir HW; Hearn TL; Centers for Disease Control, Atlanta, GA, USA; OBJECTIVE: The HTLV-I Western blot (WB) test is frequently used as a supplemental test to confirm the presence of HTLV-I antibodies detected in screening blood donor or clinical specimens. In 1989, the CDC performance evaluation program was expanded to evaluate the performance of laboratories testing for HTLV-I antibo
Immunodominant epitopes of gag and env gene encoded proteins of human T lymphotropic virus type-I and type-II.: Int Conf AIDS. 1991 Jun 16-21;7(1):169 (abstract no. M.A.1309). Unique Identifier : AIDSLINE ICA7/1130991; Lal R; Sinha S; Rudolph D; Coligan J; Folks T; Retrovirus Diseases Branch, CDC, Atlanta, GA, USA; OBJECTIVE: To map immunodominant epitopes of HTLV-I and HTLV-II by using synthetic peptides. METHODS: Twenty seven synthetic peptides representing structural motifs from the envelope of HTLV-I (env-1, 3, 5) or HTLV-II (env-2, 4, 6, 7, 12, 20-25) and core protein of HTLV-I (gag-1, 1a, 3, 5, 10, 14) or HTLV-II (gag-2, 2
Comparative molecular studies on SIVs from African green monkeys from Central and Western Africa.: Int Conf AIDS. 1991 Jun 16-21;7(1):169 (abstract no. M.A.1310). Unique Identifier : AIDSLINE ICA7/1131091; Saksena N; Muller MC; Pouliquen JF; Nerrienet E; Herve V; Durand JP; Lang MC; Digoutte J; Georges-Courbot MC; Sonigo P; et al; Institut Pasteur, Paris, France; OBJECTIVE: To study the genetic variability of SIVagm isolates from Central and Western Africa. METHODS: Cell culture, Hirt extraction of DNA, Polymerase chain reaction, Southern blot analysis and direct sequencing of PCR amplified fragments. RESULTS: Six viral isolates from AGM from
HIV-1/HTLV1 co-infection in intravenous drug users with tropical spastic paraparesis.: Int Conf AIDS. 1991 Jun 16-21;7(1):170 (abstract no. M.A.1312). Unique Identifier : AIDSLINE ICA7/1131291; Zala C; Bouzas MB; Muchinik G; Benetucci J; Cutrin JC; Da Bouza J; Hospital Francisco J. Muniz; OBJECTIVE: To describe clinical features of HIV-1/HTLV-I co-infection in intravenous drug users (IVDU). METHODS: Four white male patients IVDU HIV-1 positive who developed signs and symptoms of TSP/HAM were investigated for HTLV-I infection. Anti-HTLV-I antibody tests were performed in serum and CSF using the particle
Demonstration by PCR of HTLV variants in Italian intravenous drug users.: Int Conf AIDS. 1991 Jun 16-21;7(1):170 (abstract no. M.A.1313). Unique Identifier : AIDSLINE ICA7/1131391; Lee H; Swanson P; Mann T; Zanetti A; Abbott Laboratories, N. Chicago IL; OBJECTIVE: To genomically examine the HTLV provirus of HTLV-I/II seropositive Italian intravenous drug users (IVDU) and compare to seropositive U.S. blood donors and U.S. IVDU. METHODS: Plasma from 29 Italian and 17 U.S. HTLV-I EIA seropositive IVDU were tested for the presence of antibodies to HTLV-I/II by Western bl
Lymphocyte subsets in a naturally SIVcpz infected chimpanzee.: Int Conf AIDS. 1991 Jun 16-21;7(1):170 (abstract no. M.A.1314). Unique Identifier : AIDSLINE ICA7/1131491; Kestens L; Vanham G; Peeters M; Goilav C; van der Groen G; Piot P; Gigase P; Institute of Tropical Medicine, Antwerp, Belgium; OBJECTIVE: The immune status of a chimpanzee, naturally infected by the simian immunodeficiency virus strain cpz (SIVcpz) (1) since at least 3 years, was compared with an age matched seronegative chimpanzee. METHODS: Lymphocyte subsets were quantified with a panel of monoclonals using a whole blood lymphocyte staining
Vertical and horizontal transmission of HTLV-I in 4 generations of a Brazilian family.: Int Conf AIDS. 1991 Jun 16-21;7(1):170 (abstract no. M.A.1315). Unique Identifier : AIDSLINE ICA7/1131591; Cavalcanti M; Schechter M; Sohler M; Leite DM; Ferreira OC; AIDS Program, Federal University of Rio de Janeiro; OBJECTIVE: To investigate the familial transmission of HTLV-I and its association with Spastic Paraparesis Syndrome (SPS). METHODS: From an index case of SPS, 27 individuals of 4 generations of a family (21 blood relatives, 6 spouses) were traced, investigated for evidence of HTLV-I infection and submitted to detailed
A new retrovirus from human promonocyte cell line, HL-CZ.: Int Conf AIDS. 1991 Jun 16-21;7(1):171 (abstract no. M.A.1316). Unique Identifier : AIDSLINE ICA7/1131691; Liu WT; School of Medical Technology, National Yang-Ming College, Taipei,; Taiwan ROC; OBJECTIVE: In the past five years (1985-1991) a human promonocyte cell line, HL-CZ with CD15 marker in 90% of the cells was established. The subclones of the cell line had a new retrovirus detected. This new retrovirus is seeking recognition. METHODS: The electron microscopy was used to observe type C oncornavirus. Re
PCR analysis of HTLV-1 infection in patients with indeterminate W-blots.: Int Conf AIDS. 1991 Jun 16-21;7(1):171 (abstract no. M.A.1317). Unique Identifier : AIDSLINE ICA7/1131791; Agbalika F; Ferchal F; Honderlick P; Perol Y; Morinet F; University Paris VII - France; OBJECTIVE: To use PCR analysis for evaluating the HTLV-1 infection in patients with positive HTLV-1 ELISA and indeterminate Western blots (WB). METHODS: Genomic DNAs were extracted separately from peripheral blood lymphocytes of 10 patients who were repeate reactive HTLV-1 EIA with indeterminate WB. PCR amplification
Rarity of HIV-1/HTLV-1 coinfection in South eastern France.: Int Conf AIDS. 1991 Jun 16-21;7(1):171 (abstract no. M.A.1318). Unique Identifier : AIDSLINE ICA7/1131891; Quaranta JF; Ticchioni M; Ferri P; Cassuto JP; Dellamonica P; Bernard A; University Nice-Sophia Antipolis, CHRU Nice; OBJECTIVE: Coinfection of HIV-1 and HTLV-I has been described in intravenous drug users particularly in American and Italian studies. Indeed, contamination routes are similar. Since in Nice and neighbouring area a high prevalence of HIV-1 seropositivity is due to drug users (about 50% of HIV-infected patients), we hav
Fully artificial proteins with predetermined tertiary structure - a novel approach to vaccine design. Anti-HIV vaccine development.: Int Conf AIDS. 1991 Jun 16-21;7(1):171 (abstract no. M.A.1319). Unique Identifier : AIDSLINE ICA7/1131991; Eroshkin AM; Zhilkin PA; Shamin VV; NPO Vector, Koltsovo. Novosibirsk Region, 633159. USSR; OBJECTIVE: The aims of the work were to develop a new way for designing a vaccine in a form of protein with optimal epitope composition and predetermined tertiary structure (PTS-vaccine); to design a protein - candidate for vaccine against HIV. METHODS: Theoretical analysis of env, pol and gag protein (HIV-1) and expe
Brucella abortus (BA) stimulates normal human CD4+ and CD8+ cells and T-cells from HIV-infected persons to secrete interferon-gamma (IFNg).: Int Conf AIDS. 1991 Jun 16-21;7(1):172 (abstract no. M.A.1320). Unique Identifier : AIDSLINE ICA7/1132091; Blay R; Hernandez D; Hoffman T; Golding B; Lab. of Cell Biology, Bethesda MD 20892.; OBJECTIVE: To determine the effect of BA on CD4+ and CD8+ human T cells from normal and HIV-infected individuals in terms of IFNg production. RATIONALE: BA may be useful as a carrier for HIV-1 to elicit antibody responses in HIV-1 infected individuals with impaired CD4+ T cell function. This is based on our finding th
Antibodies to SIV envelope peptides: their role in vaccine-induced protection.: Int Conf AIDS. 1991 Jun 16-21;7(1):172 (abstract no. M.A.1321). Unique Identifier : AIDSLINE ICA7/1132191; Silvera P; Stott EJ; Rud E; Kent KA; Page M; Taffs LF; Kitchin PA; Mills KH; Chan WL; NIBSC, Potters Bar, Herts, UK; OBJECTIVE: To assay antibodies to SIV envelope peptides in infected or vaccinated macaques. To correlate antibodies with protection. METHOD: Antibodies in plasma of 29 vaccinated and 10 infected macaques were assayed by ELISA using as antigens recombinant gp140 (baculovirus generated) and peptides analogous to 5 regio
Expression of particulated HIV-1 gag and env antigens for use as immunogens.: Int Conf AIDS. 1991 Jun 16-21;7(1):172 (abstract no. M.A.1322). Unique Identifier : AIDSLINE ICA7/1132291; Gallina A; Rossi F; Sala C; Achilli G; Cattaneo E; Milanesi G; Istituto di Genetica Biochimica ed Evoluzionistica CNR, Pavia,; Italy; OBJECTIVE: The production of safe and highly immunogenic viral components to be included in a subunit vaccine represents a valuable task in view of future immunoprophylactic approaches to the prevention and treatment of HIV infection. METHODS: We have developed an Escherichia coli expression system for the synthesis o
Isolation and characterization of the env protein expressed from a recombinant adenovirus vector.: Int Conf AIDS. 1991 Jun 16-21;7(1):173 (abstract no. M.A.1324). Unique Identifier : AIDSLINE ICA7/1132491; Wilhelm J; Chanda P; Hjorth R; Dheer S; Lee S; Mizutani S; Levner M; Davis A; Hung P; Wyeth-Ayerst Research, Radnor, PA, USA; OBJECTIVE: To characterize the env protein expressed by the recombinant as part of a broader program in the development of oral HIV vaccines based on adenovirus vectors. METHODS: The vector was a non-defective type 7 adenovirus (with an E3 deletion) carrying the HIV env and rev genes in the E3 region. Human cells (lin
A highly conserved epitope on HIV-1 gag recognized by an HLA-C-restricted CD8+ CTL clone.: Int Conf AIDS. 1991 Jun 16-21;7(1):173 (abstract no. M.A.1326). Unique Identifier : AIDSLINE ICA7/1132691; Littaua RA; Oldstone MB; Takeda A; Tuazon C; Kievits F; Ennis FA; University of Massachusetts Medical Center, Worcester, MA, USA; OBJECTIVE: To identify conserved HIV-1 virus-specific human leukocyte antigen (HLA)-restricted cytotoxic T lymphocyte (CTL) epitopes for possible inclusion in vaccines against AIDS. METHODS: T cell clones were generated using PBMC from an asymptomatic HIV-1 sero-positive donor and were screened for HIV-1 specific cyto
Lack of FcR mediated immune enhancement in sera from recipients of an RGP 160 experimental HIV vaccine.: Int Conf AIDS. 1991 Jun 16-21;7(1):173 (abstract no. M.A.1327). Unique Identifier : AIDSLINE ICA7/1132791; Haubrich R; Takeda A; Koff W; Smith G; Ennis FA; University of Massachusetts Medical Center, Worcester, MA, USA; OBJECTIVE: To study FcR mediated antibody dependent enhancement (ADE) in sera from volunteers who were immunized with a recombinant gp160 candidate HIV-1 vaccine. METHOD: We have previously shown that subneutralizing concentrations of sera from HIV-1 infected patients augments HIV infection of human monocytes and a mo
An epitope map of immunity to HIV-1: a roadmap for vaccine development.: Int Conf AIDS. 1991 Jun 16-21;7(1):174 (abstract no. M.A.1328). Unique Identifier : AIDSLINE ICA7/1132891; Bradac JA; Mathieson BJ; Division of AIDS, NIAID, NIH, Bethesda, MD, USA; Evaluation of immune responses to HIV has presented a formidable challenge because of the complexities of the immune response to viral proteins. Differences in the fine specificity of epitopes recognized by T and B cells, the variable immunogenicity of specific epitopes and HIV protein due to MHC differences between i
Specific active immunotherapy in asymptomatic HIV disease using recombinant gp160.: Int Conf AIDS. 1991 Jun 16-21;7(1):174 (abstract no. M.A.1329). Unique Identifier : AIDSLINE ICA7/1132991; Tsoukas C; Falutz J; Gold P; Izaguirre C; O'Shaughnessy M; Sekaly RP; Wainberg M; Volvowitz F; Smith GE; MicroGenesys Inc., West Haven, USA; OBJECTIVE: To evaluate the safety and immunogenicity of recombinant HIV envelope precursor protein VaxSyn (R) (rgp160) HIV-1 in HIV infected asymptomatic individuals with CD4 counts greater than 500 cells/mm(3). METHODS: 20 patients (pts) were enrolled in a phase I study. Half received 160 mug (Group A) and half 320 m
PBMC from immunized chimpanzees prevent cell-to-cell transmission of HIV.: Int Conf AIDS. 1991 Jun 16-21;7(1):174 (abstract no. M.A.1330). Unique Identifier : AIDSLINE ICA7/1133091; Fultz P; Girard M; University of Alabama at Birmingham, Birmingham, AL, USA; OBJECTIVE: To determine whether PBMC from HIV-immunized chimpanzees prevent HIV transmission from PBMC of HIV-infected chimpanzees to PBMC of normal chimpanzees, thus providing a method to predict whether immunization elicits an immune response that might protect against an in vivo cell-associated virus challenge. MET
Couples of antiidiotypic monoclonal antibodies mimick HIV 1 gp120-gp41 and gp41-gp41 interactions endowed with neutralization potentials.: Int Conf AIDS. 1991 Jun 16-21;7(1):174 (abstract no. M.A.1331). Unique Identifier : AIDSLINE ICA7/1133191; Pelagi M; Lopalco L; Gasparri A; Ciccomascolo F; De Rossi A; Siccardi AG; Dipartimento di Biologia e Genetica per le Scienze Mediche,; Universita di Milano, Italy; OBJECTIVE: In order to investigate the potentials of idiotypic vaccines for HIV, we have raised antiidiotypic responses against two neutralizing monoclonal antibodies (reactive with gp120 and gp41, respectively), with the aim to obtain internal image type reagents, immunologically equivalent to neutralizable sites. ME
HIV-1 inhibition pattern of succinylated heparins.: Int Conf AIDS. 1991 Jun 16-21;7(1):175 (abstract no. M.A.1332). Unique Identifier : AIDSLINE ICA7/1133291; Lopalco L; Ciccomascolo F; Lanza P; Modena D; Zoppetti G; Beretta A; Siccardi AG; Dipartimento di Biologia e Genetica, Universita di Milano; OBJECTIVE: Aim of the study was to investigate the effects of different chemical modifications of heparin on the inhibition of HIV-1 induced syncytia formation, and to elucidate the mechanism of inhibition. METHODS: For syncytia inhibition studies, different concentrations of modified heparins were added to the syncyt
Immunogenic and antiviral properties of the recombinant HIV-like particles.: Int Conf AIDS. 1991 Jun 16-21;7(1):175 (abstract no. M.A.1333). Unique Identifier : AIDSLINE ICA7/1133391; Haffar O; Moran P; Smithgall M; Sridhar P; Jones R; Travis B; Morton WR; Dykers T; Zarling J; Hu SL; Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle,; WA, USA; OBJECTIVE: To evaluate the immunogenicity of the recombinant HIV-like particles in animal models, and to study their potential as antiviral agents. METHODS: Recombinant particles were formulated in a number of adjuvants and administered to rabbits and macaques. The amount of immunogen used was normalized with respect
Neutralizing and non-neutralizing monoclonal antibodies are generated by immunization with the principal neutralizing determinant (PND) of HIV-1-MN.: Int Conf AIDS. 1991 Jun 16-21;7(1):175 (abstract no. M.A.1334). Unique Identifier : AIDSLINE ICA7/1133491; Martinez P; Devash Y; Rubinstein A; Goldstein H; Albert Einstein College of Medicine, Bronx, NY, USA; OBJECTIVES: To determine if immunization with a 13 amino acid peptide containing the principal neutralizing domain PND of HIV-1-MN (PND-MN) could generate neutralizing antibodies and to determine the role played by epitope recognition and subclass in the capacity of monoclonal antibodies to neutralize HIV-1 infection.
Retrotransposon and retroviral cores as particulate carrier systems.: Int Conf AIDS. 1991 Jun 16-21;7(1):175 (abstract no. M.A.1335). Unique Identifier : AIDSLINE ICA7/1133591; Griffiths J; French T; Harris SJ; Holdsworth LN; Berrie EL; Hill-Perkins M; Senior JM; Burns NR; Kingsman SM; Kingsman AJ; et al; British Bio-technology Ltd., Watlington Road, Oxford, U.K.; OBJECTIVES: Neutralizing antibodies and T-cell responses have been induced following immunization with hybrid HIV:Ty virus-like particles (Ty-VLPs) (Griffiths et al, J Virology 1991 65, 450). The aim of the present study is to broaden these responses and to investigate alternative particulate carrier systems. METHODS:
Production of monoclonal antibodies to conserved sequences of the principal neutralizing determinant (PND) of HIV-1 gp120: in vitro/in vivo studies.: Int Conf AIDS. 1991 Jun 16-21;7(1):176 (abstract no. M.A.1336). Unique Identifier : AIDSLINE ICA7/1133691; Giraldo G; Castello G; Monaco M; Geretti AM; Curcio F; De Biasi S; D'Alessio V; Beth-Giraldo E; Dept. of Viral Oncology, Natl. Cancer Institute, Foundazione; Pascale, Naples, Italy; OBJECTIVE: 1. To search for an appropriate vaccine delivery system in order to develop murine monoclonal antibodies (MoAbs) to the 10 most frequent peptides of the PND of HIV-1 gp 120 with viral neutralizing activity, and 2, to set-up toxicity and efficacy tests in SCID mice with a human immunosystem. METHODS: Balb/c
Surveillance of AIDS vaccine studies in non-human primates.: Int Conf AIDS. 1991 Jun 16-21;7(1):176 (abstract no. M.A.1337). Unique Identifier : AIDSLINE ICA7/1133791; Schultz AM; Warren J; Vaccine Research & Development Branch, Division of AIDS, NIAID,; NIH, Bethesda, Maryland; Relevant animal models essential to AIDS vaccine development are now well-developed. Simian immunodeficiency virus (SIV) originating from the sooty mangabey reliably infects rhesus and cynomolgus macaques, and causes disease in the rhesus. This surrogate model for human AIDS is now well-established and has already pro
HIV-1 ISCOMS as a potent and putative vaccine against AIDS.: Int Conf AIDS. 1991 Jun 16-21;7(1):176 (abstract no. M.A.1338). Unique Identifier : AIDSLINE ICA7/1133891; Marlas G; Institut Pasteur; OBJECTIVE: To elaborate a potent preventive and curative vaccine against the human AIDS virus. METHODS: Whole HIV-1BRU, recombinant gp160 (PMSV) or a synthetic peptide (ANRS) from the V3 loop of gp160 were incorporated into ISCOMS (Immuno Stimulating COMplexeS) and injected separately into mice. Before incorporation i
Immunization of rhesus monkeys (macaca mulatta) with a subunit vaccine of SIVmac.: Int Conf AIDS. 1991 Jun 16-21;7(1):176 (abstract no. M.A.1339). Unique Identifier : AIDSLINE ICA7/1133991; Stahl Hennig C; Nick S; Coulibaly C; Voss G; Luke W; Hunsmann G; German Primate Center, Gottingen, FRG; OBJECTIVE: To develop a vaccine against AIDS on the basis of a macaque model. METHODS: 7 rhesus monkeys were immunized intramuscularly with 140 mug per dose of tween-ether treated SIVmac251/32H adsorbed to an aluminum hydroxide adjuvant on months 0, 1, 2 and 4, respectively. Three control animals received the adjuvant
An HIV-1 neutralizing monoclonal antibody recognizes an epitope in the V2 region of gp120.: Int Conf AIDS. 1991 Jun 16-21;7(1):177 (abstract no. M.A.1340). Unique Identifier : AIDSLINE ICA7/1134091; Fung M; Sun C; Gordon W; Liou R; Daar E; Sun W; Chang N; Chang TW; Ho D; Tanox Biosystems, Inc., Houston, TX, USA; OBJECTIVE: To characterize murine monoclonal antibodies (MAbs) reactive with the variable (V2) region of HIV-1 gp120. METHOD: BALB/c mice were immunized s.c. with purified HTLV-IIIB gp120. Splenocytes were isolated and fused with Sp2/0 myeloma cells. MAbs produced were tested for reactivity with gp120 by ELISA and rad
Continuous large scale HIV production in artificial capillary systems.: Int Conf AIDS. 1991 Jun 16-21;7(1):177 (abstract no. M.A.1341). Unique Identifier : AIDSLINE ICA7/1134191; Gilljam G; Hinkula J; Makitalo B; Department of Virology, Stockholm, Sweden; OBJECTIVE: To obtain a continuous HIV production in an artificial capillary system with high titer. METHODS: A hollow fiber bioreactor was used. The hollow fiber had membranes with a surface of 1.1 m(2) and a molecular weight cutoff of 10000 daltons. The system contains a cartridge for gas exchange and pH , temperatur
Progress in the development of a P17 based HIV vaccine: safety and potential efficacy of the HGP-30 synthetic peptide vaccine in humans.: Int Conf AIDS. 1991 Jun 16-21;7(1):177 (abstract no. M.A.1342). Unique Identifier : AIDSLINE ICA7/1134291; Goldstein AL; Naylor PH; Sarin PS; O'Toole C; Kirkley JE; Rios A; Zagury D; Achour A; Gazzard B; Gibbs CJ; et al; The George Washington University Med. Ctr., Washington, D.C. USA; OBJECTIVE: Evaluation of the safety and potential efficacy of an HIV p17 based synthetic peptide vaccine in humans. METHODS: HIV seronegative volunteers received a synthetic peptide candidate HIV-1 gag vaccine (HGP-30-KLH/Alum) in a phase 1 clinical trial. B cell response to antigen was evaluated by ELISA and T-cell r
Active immunization of recombinant gp160/p24 vaccine (VAXSYN(R)) in HIV-infected individuals: a community-based phase I/II trial of toxicity and immunogenicity.: Int Conf AIDS. 1991 Jun 16-21;7(1):177 (abstract no. M.A.1343). Unique Identifier : AIDSLINE ICA7/1134391; Blick G; Buchanan S; Diamond M; Smith G; Volvovitz F; Greenwich Hospital, Yale University, Greenwich, CT., USA; OBJECTIVE: To evaluate the toxicity and immunogenicity of recombinant gp160 envelope precursor and p24 core proteins in HIV-infected individuals. METHODS: Patient Selection: 30 individuals with HIV infection were randomly immunized regardless of CD4 cell count, with baculovirus expressed gp160 and p24 vaccines. (VAXSY
(Vaxsyn HIV-1) elicits a pattern of HIV-specific immune responses distinct from natural infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):178 (abstract no. M.A.1344). Unique Identifier : AIDSLINE ICA7/1134491; Smith G; Lane HC; Tsoukas C; Redfield R; Hackett C; Volvovitz F; MicroGeneSys, Inc., Meriden, CT, USA; OBJECTIVES: To elucidate the pattern of HIV-specific immunity in HIV-seronegative volunteers and HIV-infected patients following immunization with VaxSyn HIV-1, a recombinant gp160 vaccine (rgp160). METHODS: Phase I/II clinical trials of rgp160 (MicroGeneSys, Inc.) produced in a baculovirus expression system and formu
Isolated from sea cucumbers.: Int Conf AIDS. 1991 Jun 16-21;7(1):178 (abstract no. M.A.1345). Unique Identifier : AIDSLINE ICA7/1134591; Handa A; Numata K; Yamanaka E; Hoshino H; Department of Hygiene, Gunma University School of Medicine, Japan; OBJECTIVE: Glycosaminoglycan extracted from sea cucumbers, Stichopus japonicus Selenka, has anticoagulant, antithrombotic and platelet-aggregating activities. It has already been administered to humans in China as an anticoagulant. We produced and purified depolymerized fragments (D
A gp120/PND consensus peptide (PNDC) and derived P55/PNDC recombinant vaccinia viruses as vaccine candidates.: Int Conf AIDS. 1991 Jun 16-21;7(1):178 (abstract no. M.A.1346). Unique Identifier : AIDSLINE ICA7/1134691; Wagner R; Fliebetabach H; Modrow S; Boltz T; v Brunn A; Wolf H; Max V. Pettenkofer Institute, LMU, Munich, FRG; OBJECTIVE: To develop an HIV-1 vaccine candidate based on a rationally designed consensus sequence of the gp120 principal neutralizing determinant V3 (PNDc) inserted into the HIV-1 group specific antigen (gag). RESULTS AND METHODS: Conventional HIV-1 vaccine strategies, based on rgp160/120 or inactivated virus prepara
Particulate lipoprotein/protein structures of the hepatitis B virus (HBsAG, HBcAG) as carriers for HIV epitopes: construction and evaluation.: Int Conf AIDS. 1991 Jun 16-21;7(1):178 (abstract no. M.A.1347). Unique Identifier : AIDSLINE ICA7/1134791; von Brunn A; Wagner R; Fliebetabach H; Wolf H; Schodel F; Max-von-Pettenkofer-Institut, Ludwig-Maximilians-Universitat,; Munchen, FRG; The utilization of peptides containing immunologically relevant epitopes as vaccines is often limited by the lack of suitable carrier systems which ideally would possess an intrinsic adjuvant potential. The development of subunit vaccines against HIV requires both the identification of immunologically relevant epitope
Design of conformationally constrained peptide tertiary structural templates (PTST) for AIDS vaccine development.: Int Conf AIDS. 1991 Jun 16-21;7(1):179 (abstract no. M.A.1348). Unique Identifier : AIDSLINE ICA7/1134891; Komoriya A; Laboratory of Cell Biology, Division of Cytokine Biology, Center; for Biologics Evaluation and Review, FDA, Bethesda, Maryland USA; OBJECTIVE: A successful peptide vaccine must overcome several problems that currently exist. Specifically, it must be able to elicit antibodies which can cross-react with the native protein, its antigenic determinants must be the same as the desired viral neutralizing epitopes, and the vaccine must induce active immun
Structural analysis of a V3-consensus sequence of gp 120/HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):179 (abstract no. M.A.1349). Unique Identifier : AIDSLINE ICA7/1134991; Borbe H; Wagner R; Holak T; Wolf H; Modrow S; Max von Pettenkofer Institute, Ludwig-Maximilians-Universitat,; Munchen, FRG; OBJECTIVE: The V3 domain of gp120 is the only antigenic region not modified by carbohydrates. Neutralizing antibodies to V3 are present in HIV-patients and can be induced in experimental systems. The high degree of sequence variability makes it difficult to include this compound in general strategies for vaccine desig
Immunization of primates with recombinant HIV-1 gp120 induces broadly neutralizing activity that cannot be explained by V3 sequence homologies.: Int Conf AIDS. 1991 Jun 16-21;7(1):179 (abstract no. M.A.1350). Unique Identifier : AIDSLINE ICA7/1135091; Steimer KS; Nara P; Brooks E; Sakamoto D; Eichberg J; Haigwood N; Chiron Corporation, Emeryville, California, USA; OBJECTIVE: To examine the ability of recombinant HIV-1 gp120 to generate neutralizing activity in primates directed to conformation-dependent epitopes. METHODS: The HIV-SF2 env gene was engineered for the expression of native, glycosylated recombinant gp120 in mammalian cells. The protein (rgp120SF2) was used to immun
Induction of neutralizing activity to HIV with synthetic peptides.: Int Conf AIDS. 1991 Jun 16-21;7(1):179 (abstract no. M.A.1351). Unique Identifier : AIDSLINE ICA7/1135191; Morys-Wortmann C; Jurkiewicz E; Hunsmann G; Kleinschmidt A; Erfle V; Hartmann H; Creutzfeldt W; Schmidt WE; Zentrum Innere Medizin, Georg-August-Universitat, Gottingen; Germany; OBJECTIVE: Synthetic peptides were used to elicit anti-HIV-neutralizing activity in rabbits. METHODS: Peptides corresponding to the hypervariable loop V3 of gp120 of different HIV isolates were synthezised by Fmoc-chemistry. Mixed peptides were synthezised using two or three amino acids in one reaction. Rabbits were i
Clinical trial phase I for selection of an AIDS vaccine.: Int Conf AIDS. 1991 Jun 16-21;7(1):180 (abstract no. M.A.1353). Unique Identifier : AIDSLINE ICA7/1135391; Picard O; Bernard J; Halbreich A; Carelli C; Bizzini B; Achour A; Lebas J; Frottier J; Bigel P; Salaun JJ; et al; Universite Pierre et Marie Curie, Paris, France; A clinical trial phase I was performed in seronegative volunteers at Hospital Saint Antoine in order to define a vaccine preparation available for mass use, which could induce a protective in vitro immune reaction similar to the candidate vaccine prototype previously described(a,b). The latter was obtained by using r
A novel multistep procedure for inactivating and adjuvanting HIV.: Int Conf AIDS. 1991 Jun 16-21;7(1):180 (abstract no. M.A.1354). Unique Identifier : AIDSLINE ICA7/1135491; Race E; Dourmashkin RR; Broadhurst K; Ball A; Frezza P; Oxford JS; The London Hospital Medical College, London, England; OBJECTIVES: To develop a safe inactivated whole HIV vaccine with virion incorporated adjuvant. METHODS: Inactivating agents, beta-propiolactone (BPL), binary ethyleneimine (BEI), cholates, digitonin, nucleases, and aldehydes were applied, individually and in series, to HIV-1 RF in culture supernatants harvested from c
Accelerating HIV vaccine development: a global applied research strategy.: Int Conf AIDS. 1991 Jun 16-21;7(1):180 (abstract no. M.A.1355). Unique Identifier : AIDSLINE ICA7/1135591; Koff WC; Schultz AM; Mathieson BJ; Fast PE; Detrick BJ; Bradac JA; Lawrence DN; Vaccine R&D Branch, Division of AIDS, NIAID, NIH, Bethesda,; Maryland, USA; During the past twelve months, significant scientific advances in preclinical and clinical testing of HIV vaccines have demonstrated that development of safe and effective HIV vaccines will be feasible. The timetable for successfully developing, licensing, and delivering efficacious HIV vaccines would be markedly acce
Analysis of the serological response of rhesus monkeys immunized with HIV-1 recombinant envelope proteins.: Int Conf AIDS. 1991 Jun 16-21;7(1):181 (abstract no. M.A.1356). Unique Identifier : AIDSLINE ICA7/1135691; Francotte M; Bruck C; De Wilde M; Van Opstal O; Culp J; Horton L; Robinson E; Thiriart C; SmithKline Beecham Biologicals, Rixensart, Belgium; OBJECTIVE: Comparison of immunogenicity and functional immune responses between recombinant gp160 (rgp160) and gp120 (rgp120). METHODS: Two groups of 4 Rhesus monkeys were immunized with HIV-1 rgp160 (IIIB isolate) from mammalian cells, and gp120 obtained from drosophila cells, respectively. Sera were characterized fo
Linear polymers of CD4 as polyvalent molecular decoys.: Int Conf AIDS. 1991 Jun 16-21;7(1):181 (abstract no. M.A.1357). Unique Identifier : AIDSLINE ICA7/1135791; Smythe JA; Nardelli B; Chatterjee P; Gallo RC; Gershoni JM; LTCB, NCI, National Institutes of Health, Bethesda, MD. USA; OBJECTIVE: To produce recombinant linear polymers of the gp120-binding domain of CD4. To test the gp120-binding capacity of these recombinant polymers, and assess the potential of these polyvalent molecular decoys for AIDS therapy. METHODS: The gp120-binding site is located within the V1 domain of CD4. We engineered
Protection of macaques with a vaccine of SIV envelope peptides.: Int Conf AIDS. 1991 Jun 16-21;7(1):181 (abstract no. M.A.1358). Unique Identifier : AIDSLINE ICA7/1135891; Eddy GA; Shafferman A; Jahrling PB; Lewis MG; Benveniste RE; Eden-McCutchan F; Burke DS; Henry M Jackson Foundation; OBJECTIVE: Evaluate the efficacy of conserved, hydrophilic envelope peptides as immunogens against SIV associated disease in rhesus macaques. METHODS: Rhesus were immunized with a mixture of four peptides, 10 to 21 amino acids in length, of SIV envelope regions (SIV-88, SIV-500, SIV-582 and SIV-647) given as recombina
Diagnostic utility of lumbar puncture (LP)in HIV-infected individuals.: Int Conf AIDS. 1991 Jun 16-21;7(1):182 (abstract no. M.B.2000). Unique Identifier : AIDSLINE ICA7/1200091; Burack JH; McGuire D; Hollander H; University of California, San Francisco, CA, USA; OBJECTIVE: To evaluate the yield of LP for common HIV-related indications. METHODS: Retrospective chart review of LP s for one or more of the following indications: headache, fever, altered mental status. RESULTS: One hundred forty six LP s were reviewed. Indications included headache - 90, fever - 89, altered mental
5 FU and clindamycin for treatment of cerebral toxoplasmosis.: Int Conf AIDS. 1991 Jun 16-21;7(1):182 (abstract no. M.B.2003). Unique Identifier : AIDSLINE ICA7/1200391; Dhiver C; Dupire B; Somma H; Poizot-Martin I; Bouabdallah R; Gastaut JL; Gastaut JA; Centre d'investigation et de soins de l'immunodeficience; humaine, hopital Salvator, Marseille; OBJECTIVES: A synergistic activity of the association 5 - Fluorouracil (5FU) and Clindamycin (CL) against Toxoplasma gondii in vitro is suggested. Patients (p) who could not receive classical treatment for cerebral toxoplasmosis (T) because of bad tolerance, received this combination. METHODS: 8 p enrolled : 6 p once,
Morphological findings on peripheral nerve biopsies in 15 patients with HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):183 (abstract no. M.B.2004). Unique Identifier : AIDSLINE ICA7/1200491; Vital A; Vital C; Julien J; Beylot M; Delors B; Bloch B; Bordeaux II University, France; Peripheral nerve biopsies from 15 patients with HIV infection were studied by light and electron microscopic examination (EME), and by direct immunopathologic examination (DIPE) with anti-immunoglobulin sera. There were 3 otherwise asymptomatic patients, 2 with ARC and 10 with AIDS. Two cases (1 asymptomatic, 1 ARC) p
Cerebral venous thrombosis in three AIDS-patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):183 (abstract no. M.B.2005). Unique Identifier : AIDSLINE ICA7/1200591; Fernandez-Martin J; Raguin G; Perronne V; Longuet P; Hercot O; Leport C; Vilde JL; Hospital Bichat-Claude Bernard, Paris, France; OBJECTIVE: To describe the occurrence of cerebral venous thrombosis (CVT), a complication which has not been previously reported in AIDS patients (pts). METHODS: Diagnosis of CVT was based on the occurrence of acute neurological signs and confirmed by cerebral angiography and/or brain CT scan. RESULTS: CVT was diagnos
Effect of peptide T on AIDS dementia complex (ADC): correlation of PET scans and cognitive domains.: Int Conf AIDS. 1991 Jun 16-21;7(1):183 (abstract no. M.B.2006). Unique Identifier : AIDSLINE ICA7/1200691; Heseltine P; Eaton E; Buchsbaum M; Parker E; McGrail M; Leedom J; Bridge P; University of Southern California, Los Angeles, USA; OBJECTIVE: Phase I Peptide T (D-ala-1-peptide-T-amide) studies suggest it improves neuropsychologic (NP) test scores in persons with HIV and cognitive impairment. We report a pilot study of Peptide T s effect on severely HIV cognitively impaired persons, i.e. with ADC. METHODS: Five persons with ADC (Price classificat
Survival trends and cryptococcal meningitis.: Int Conf AIDS. 1991 Jun 16-21;7(1):183 (abstract no. M.B.2007). Unique Identifier : AIDSLINE ICA7/1200791; Waskin H; Ingram CW; Cameron M; Drew R; Bartlett JA; Duke University Medical Center, Durham North Carolina USA; OBJECTIVE: To determine prognostic indicators for long-term survival of HIV-infected persons with cryptococcal meningitis. METHODS: Longterm followup of a cohort of 55 HIV+ persons diagnosed with cryptococcal infection at Duke University from 1985-1990. RESULTS: We followed until present or death, 55 HIV+ persons with
AIDS in presenile age: frequency of AIDS-dementia symptoms.: Int Conf AIDS. 1991 Jun 16-21;7(1):184 (abstract no. M.B.2008). Unique Identifier : AIDSLINE ICA7/1200891; Cairoli S; Brustia R; Bono G; Merlo P; Minoli L; Grossi P; Neuro-AIDS Unit, IRCCS C.Mondino, University of Pavia, Italy; OBJECTIVE: Within a series of n.240 HIV-infected pts observed between Jan 87 and Dec 90, n.19 (8%) were in the presenile age. Eventual age-related differences in the evolution of HIV-disease, and CNS involvement are discussed. METHODS: Selection of HIV-patients aged over 50 yrs; clinical staging (CDC); evaluation of
Developmental and neurological status of infants born to HIV infected mothers: birth to 18 months.: Int Conf AIDS. 1991 Jun 16-21;7(1):184 (abstract no. M.B.2009). Unique Identifier : AIDSLINE ICA7/1200991; Mitchell WG; Kovacs AS; University of Southern California School of Medicine, Los; Angeles, CA, USA; We examined infants of HIV+ mothers one or more times: Group 1 consisted of 26 infants between age 2 weeks and age 6 months; Group 2 consisted of 21 infants between 6 months and 18 months. Current HIV infection status is classified as definitely infected CDC classes P1 and P2, definitely uninfected - CDC class P3, or
Archaic reflexes in neurologically asymptomatic HIV positive subjects.: Int Conf AIDS. 1991 Jun 16-21;7(1):184 (abstract no. M.B.2010). Unique Identifier : AIDSLINE ICA7/1201091; Orefice G; Troisi E; Carrieri PB; Chirianni A; DeMarinis T; Marasco G; Rubino S; Campanella G; II e I Clinica Neurologica, II Facolta di Medicina, Universita; Federico II, Napoli, Italy; OBJECTIVE: To identify early markers of central nervous system (CNS) involvement in asymptomatic HIV positive individuals, by the standard neurological examination. METHODS: 106 asymptomatic HIV positive subjects with prevalent past history of i.v. drug abuse (group 1), underwent a clinical examination to ascertain th
An open study on Zidovudine therapy for ADC: one year follow-up.: Int Conf AIDS. 1991 Jun 16-21;7(1):184 (abstract no. M.B.2011). Unique Identifier : AIDSLINE ICA7/1201191; Tozzi V; Galgani S; Gerace C; Narciso P; Balestra P; Sette P; Meccia A; Pau F; Visco G; USL RM10: L. Spallanzani Hosp., Rome, Italy; OBJECTIVE: To assess long term efficacy and tolerance of Zidovudine ( AZT ) therapy for AIDS dementia complex (ADC). METHODS: 30 patients (pts) with ADC (11 stage 1, 6 st.2, 9 st.3, 4 st.4 according to Price) were trd
Gender differences in neuropsychological vulnerability of children with vertically-acquired HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):185 (abstract no. M.B.2012). Unique Identifier : AIDSLINE ICA7/1201291; Moss H; Brouwers P; Wolters P; El-Amin D; Butler K; Pizzo P; National Cancer Institute, Pediatric Branch, Bethesda, MD, USA; OBJECTIVE: To compare female and male children with vertically-acquired HIV infection on tests of general intelligence and on some indices of the natural history of the disease (age, encephalopathy). METHODS: Forty-two female (mean age=3.09 +/- .41 yrs) and 41 male (mean age=2.0 +/- .24 years) pediatric patients with d
Pyrimethamine-plasma-concentrations during prophylaxis of cerebral toxoplasmosis (CTX) in patients with advanced HIV-infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):185 (abstract no. M.B.2014). Unique Identifier : AIDSLINE ICA7/1201491; Klinker H; Joeres R; Richter E; Medizinische Universitatsklinik D-8700 Wurzburg, FRG; OBJECTIVE: Pyrimethamine (pyr) alone or in combination is part of prophylaxis against cerebral toxoplasmosis in AIDS. We have measured dose dependent pyr-plasma-concentrations. METHODS: 34 patients with advanced HIV-infection (T4-cells less than 250/mul) were treated 1-29 (9, 8+6, 6) months; 333 patient-months were obd
Cellular infiltrates in HIV-1 associated neuropathies.: Int Conf AIDS. 1991 Jun 16-21;7(1):185 (abstract no. M.B.2015). Unique Identifier : AIDSLINE ICA7/1201591; Hogenhuis JA; Ratinahirana H; Henin D; Chaunu MP; Hauw JJ; Hopital de la Salpetriere, Paris, France; OBJECTIVE: To classify by immunocytochemistry the composition of the inflammatory infiltrates in the peripheral nerves of HIV-1 infected patients. METHODS: In 9 selected HIV+ patients [5 distal symmetrical polyneuropathies (1 otherwise asymptomatic carrier and 4 AIDS), 3 acute or chronic inflammatory demyelinating pold
Clinical manifestations, diagnosis and outcome of tuberculous meningitis (TM) in patients (PTS) infected with human immunodeficiency virus (VIH+).: Int Conf AIDS. 1991 Jun 16-21;7(1):186 (abstract no. M.B.2016). Unique Identifier : AIDSLINE ICA7/1201691; Berenguer J; Moreno S; Laguna F; Adrados M; Vicente T; Gazapo E; Bouza E; Hosp. G. Maranon, Madrid, Espana; OBJECTIVE: The study of clinical manifestations, diagnosis and outcome of TM in HIV+ PTS. METHODS: Review of cases that fulfilled the following criteria: 1) Isolation of Mycobacterium tuberculosis from CSF, 2) HIV Seropositivity. RESULTS: Between 1985-90, TM has been diagnosed in 37 HIV+ PTS. in two hospitals in Madrid
CNS toxoplasmosis in AIDS patients: a clinical, pathological, serological and radiological review of 39 cases.: Int Conf AIDS. 1991 Jun 16-21;7(1):186 (abstract no. M.B.2017). Unique Identifier : AIDSLINE ICA7/1201791; Speirs G; Mijch AS; Lucas CR; Druce JD; Hayes K; Fairfield Hospital, Melbourne, Victoria, Australia; OBJECTIVE: To review all AIDS patients with a diagnosis of toxoplasmosis who presented between March 1986 and February 1990, and in particular to assess the usefulness of serology in diagnosis. METHODS: Clinical case histories, radiological reports, serological results and histopathological findings were assessed. Antd
Monoaminergic tranmitter systems during HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):186 (abstract no. M.B.2018). Unique Identifier : AIDSLINE ICA7/1201891; Larsson M; Hagberg L; Forsman A; Norkrans G; Dept of Psychiatry, Lillhagen Hospital, University of Goteborg,; Sweden; OBJECTIVE: Analyses of neurobiological parameters measured in blood and CSF and their possible relationships to specific neuropsychiatric symptoms during all stages of HIV infection. METHODS: Ninety individuals at various stages of HIV infection have been investigated with biochemical analyses (HPLC) and clinical stagd
HIV-1 infection and autonomic nervous system.: Int Conf AIDS. 1991 Jun 16-21;7(1):186 (abstract no. M.B.2019). Unique Identifier : AIDSLINE ICA7/1201991; Gastaut JL; Pouget J; Valentin P; Lafeuillade A; Dhiver C; Gastaut JA; Department of Neurology, Hopital Sainte Marguerite, Marseille,; France; OBJECTIVE: Symptomatic cases of autonomic nervous system (ANS) dysfunction are rare. Nevertheless some systematic functional studies of ANS in HIV+ patients have revealed the possibility of a subclinical dysautonomia. We wanted to determine its prevalence in HIV+ patients. METHODS: At the present time we have studied d
Neuropsychological assessment of HIV seropositive hemophiliacs.: Int Conf AIDS. 1991 Jun 16-21;7(1):187 (abstract no. M.B.2020). Unique Identifier : AIDSLINE ICA7/1202091; Kokkevi A; Hatzakis A; Maillis A; Pittadaki J; Zalonis J; Samartzis D; Touloumi G; Mandalaki T; Stefanis C; Department of Psychiatry, Athens University Medical School,; Athens, Greece; OBJECTIVE: The present study aims at evaluating: a. whether asymptomatic or symptomatic (excluding ARC and AIDS) stages of HIV infection are associated with cognitive impairment and b. whether any cognitive impairment in HIV+ individuals constitutes a progressive phenomenon or is associated with the appearance of sympd
Neurologic disease in Ugandan AIDS patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):187 (abstract no. M.B.2021). Unique Identifier : AIDSLINE ICA7/1202191; Baingana G; Katabira E; Hellman N; Makerere University Kampala, Uganda; OBJECTIVES: To evaluate the prevalence, clinical manifestations and cerebrospinal fluid findings of Ugandan patients in Mulago Hospital with HIV infection. To determine potentially treatable illness using simple diagnostic techniques. METHODS: A descriptive study of a sequential, convenience sample of patients admitted
Subtle neuropsychological impairment is related to progression to illness in initially asymptomatic HIV-1 positive intravenous drug users (IVDUs).: Int Conf AIDS. 1991 Jun 16-21;7(1):187 (abstract no. M.B.2022). Unique Identifier : AIDSLINE ICA7/1202291; McKegney FP; Silberstein C; O'Dowd MA; Cochrane K; Hartel D; Schoenbaum E; Friedland G; Albert Einstein College of Medicine/Montefiore Medical Center,; New York, NY USA; OBJECTIVE: To examine whether subtle cognitive impairments in asymptomatic HIV positive IVDUs predict development of symptomatic HIV related illness (ARC). METHODS: In 1985/86, neuropsychological (NP) tests were administered to 64 asymptomatic HIV+ IVDUs with follow-up at a mean of 45.6 months (range 1-54 mos) for thed
Effect of Zidovudine (ZID) on early neurological manifestations of HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):187 (abstract no. M.B.2023). Unique Identifier : AIDSLINE ICA7/1202391; Koralnik IJ; Burkhard P; Ruiz-Scrignari V; Mayer E; Vibert D; Volanschi M; Hirschel B; Clinique Medicale Therapeutique, Hopital Cantonal; Universitaire, Geneva, Switzerland; OBJECTIVE: We have shown previously (NEJM 1990, 323:864-870) that 67% of asymptomatic HIV-seropositives have abnormalities in electrophysiologic tests. This trial aimed to determine whether these could be affected by treatment with zid. METHODS: 7 asymptomatic patients, known to have abnormal electrophysiologic tests d
Focal pontine leukoencephalopathy presenting with CNS signs in an AIDS patient.: Int Conf AIDS. 1991 Jun 16-21;7(1):188 (abstract no. M.B.2024). Unique Identifier : AIDSLINE ICA7/1202491; Farthing C; Cravioto U; Seidlin M; New York University Medical Center, New York, NY, USA; Focal positive leukoencephalopathy (FPL) a recently described pathological entity, distinct from central pontine myelinosis and progressive multifocal leukoencephalopathy (PML), has been described in AIDS patients and other immunocompromised patients but only at autopsy without a clinical correlation. We report the fid
Relating subjective sleep complaints, immune status, and psychological state in HIV.: Int Conf AIDS. 1991 Jun 16-21;7(1):188 (abstract no. M.B.2025). Unique Identifier : AIDSLINE ICA7/1202591; Brown S; Atkinson H; Malone J; Chandler J; McCutchan A; Grant I; University of California at San Diego, San Diego, California; OBJECTIVE: To examine the relationship between sleep complaints, immune status, and psychological states in HIV illness. METHOD: Subjects were categorized as CDC 0 (N=35), CDC II & III (N=95), and CDC IV (N=60), on entry into a longitudinal study of CNS effects of HIV infection. Enrollees were excluded for drug and
Investigation - one year follow-up.: Int Conf AIDS. 1991 Jun 16-21;7(1):188 (abstract no. M.B.2026). Unique Identifier : AIDSLINE ICA7/1202691; Catalan J; Klimes I; Bond A; Garrod A; Day A; Elcombe S; Hodges S; Rizza C; Charing Cross and Westminster Medical School, London, UK; OBJECTIVE: To study the prevalence, nature and possible development of psychosocial problems in HIV+ men with haemophilia in a 3 year prospective study. Twelve month follow-up data is given. METHODS: Twenty-two HIV+ and 29 HIV- haemophiliacs were evaluated to assess their mental status (PSE, POMS, Beck Hopelessness Sc
Treatment of presumed central nervous system toxoplasmosis with doxycycline.: Int Conf AIDS. 1991 Jun 16-21;7(1):188 (abstract no. M.B.2027). Unique Identifier : AIDSLINE ICA7/1202791; Pope-Pegram L; Gathe J Jr; Bohn B; Piot D; Stool E; Speial Diseases Unit, Park Plaza Hospital, Houston, Texas, USA; OBJECTIVE: To describe the efficiency of Doxycycline in AIDS patients with presumed central nervous system toxoplasmosis. METHODS: Prospective evaluation of two AIDS patients in which Doxycycline was utilized in therapy of presumed central nervous system toxoplasmosis (P-CNS-T). Appropriate history, physical examinati
Psychiatric morbidity in early HIV infection in women: results of a 3.5 year prospective study.: Int Conf AIDS. 1991 Jun 16-21;7(1):189 (abstract no. M.B.2028). Unique Identifier : AIDSLINE ICA7/1202891; Brown GR; Rundell JR; Seal G; Plotnick E; Wilford Hall Medical Center, San Antonio, Texas, USA; OBJECTIVE: To prospectively assess psychiatric morbidity in women during the natural history of early (non-AIDS) HIV infection detected as part of a mandatory HIV testing program in the United States Air Force. METHODS: All active duty women testing positive since 1987 have been prospe
Immediate effectiveness of zidovudine in a child with severe HIV-related encefalopathy.: Int Conf AIDS. 1991 Jun 16-21;7(1):189 (abstract no. M.B.2029). Unique Identifier : AIDSLINE ICA7/1202991; Bini E; Mannelli F; Galli L; de Martino M; Vierucci A; Division of Pediatric Infections Diseases, Department of; Pediatrics, University of Florence, Italy; CASE REPORT: The rapid effectiveness of zidovudine ( AZT ) treatment in a 3-year child with HIV-related encefalopathy is reported. The patient was admitted because of unexplained anemia, persistent candidiasis and hepatomegaly. Presence of HIV-1 antibod
Clinical course of HIV-infection in 100 patients with subclinical motor deficits.: Int Conf AIDS. 1991 Jun 16-21;7(1):189 (abstract no. M.B.2030). Unique Identifier : AIDSLINE ICA7/1203091; Arendt G; Hefter H; Jablonowski H; Freund HJ; Department of Neurology and Medicine, University of Dusseldorf; OBJECTIVE/METHODS: 77% of 100 HIV-positive individuals of all CDC-stages - asymptomatic in neurological and psychiatric examinations - showed abnormalities in motor testing parameters (tremor peak frequency = TPF, most rapid alternating index finger movements = MRAM and most rapid index finger extensions = MRC) which
Occurrence of acute mania in two AIDS patients during dideoxyinosine treatment at a dose of 750mg/24h.: Int Conf AIDS. 1991 Jun 16-21;7(1):189 (abstract no. M.B.2031). Unique Identifier : AIDSLINE ICA7/1203191; Orth JP; Ollivier B; Vinti H; Cassuto JP; Groupe d'Etudes et de Recherche Clinique, Biologique et; Therapeutique, Faculte de Medecine, Nice, France; OBJECTIVE: Occurrence of mania in patients with no prior history of this disorder. METHODS: Clinical observations in a 25 year old woman and a 20 year old man by a psychiatrist and psychologist from outside our service. RESULTS: After receiving a daily dose of 750 mg of ddI for 3 months, the appearance of mania was no
Incidence and clinical significance of polyomaviruses in brain tissue from patients with HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):190 (abstract no. M.B.2032). Unique Identifier : AIDSLINE ICA7/1203291; Ingrand D; Moret H; Sazdovitch V; Meharzi J; Huraux JM; Laboratoire de Virologie du CERVI, Hopital de la Pitie, Paris,; France; OBJECTIVE: To determine the incidence and the clinical significance of JC viruses detected in frozen CNS samples obtained at autopsy from 50 adult AIDS patients. METHODS: To detect JC DNA genome, 50 frozen tissues were tested with two techniques: i) Southern blot transfer and hybridization with labeled nucleic acid pr
Hyaline globuli showing AZT positivity in the central nervous system (CNS): Int Conf AIDS. 1991 Jun 16-21;7(1):190 (abstract no. M.B.2033). Unique Identifier : AIDSLINE ICA7/1203391; Artigas J; Grosse G; Niedobitek F; Arasteh K; L'age M; Averdunk R; Hornscheidt M; Institute of Pathology, Auguste-Viktoria-Krankenhaus,; Rubensstrasse 125, D-1000 Berlin 41, FRG; OBJECTIVE: To study the morphological changes in the CNS after treatment with AZT . METHODS: The brains and spinal cords of 40 AIDS-patients who died during 1990 were extensively examined by means of conventional histology and immunohistochemistry. Bio
Cerebrospinal fluid (CSF) beta-2 microglobulin (beta2m) is increased in non-demented neurocognitively-impaired men with HIV-1 infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):190 (abstract no. M.B.2034). Unique Identifier : AIDSLINE ICA7/1203491; McCutchan JA; Gulevich S; Durand D; Velin R; Heaton R; Thal L; Villasana D; Kaplanski M; Malone J; Chandler J; et al; HIV Neurobehavioral Research Center, University of California,; San Diego, CA, USA; OBJECTIVE: Serum beta2m is a prognostic indicator in HIV infection. CSF beta2m is increased in HIV-associated dementia , but its relationship to subtle neuropsychological (NP) deficits is not known. We explored associations between serum and CSF beta2m, CSF inflammation, and NP perf
Nervous system involvement in HIV patients: somatosensory and event related potentials study.: Int Conf AIDS. 1991 Jun 16-21;7(1):190 (abstract no. M.B.2035). Unique Identifier : AIDSLINE ICA7/1203591; Grippo A; Ghidini P; Schiavone V; Ragazzoni A; Mecocci L; Balocchini E; Meli M; Mazzotta F; Vivarelli A; Bartolozzi D; et al; Depart. of Neurology University of Florence; Infectious Disease; Division; Florence ITALY; OBJECTIVE: Both alterations of somatosensory pathways and of a cognitive functions have been described in HIV+ patients. Electrophysiological techniques were proposed for detecting subclinical neurological deficit during HIV1 infection. Their predictive use of clinical evolution in HIV1 infection could be presumed. ME
Decline of AIDS dementia complex notifications in the period 1989-1990 in Italy: possible role of early zidovudine treatment.: Int Conf AIDS. 1991 Jun 16-21;7(1):191 (abstract no. M.B.2036). Unique Identifier : AIDSLINE ICA7/1203691; Chiesi A; Agresti MG; Dally LG; Zaccarelli M; Tomino C; Floridia M; Vella S; Centro Operativo AIDS, Istituto Superiore di Sanita, Rome, Italy; OBJECTIVE: To study the incidence of AIDS dementia complex (ADC) in groups of patients who acquired infection through different risk behaviours, and to evaluate the possible role of zidovudine ( AZT ) treatment in pr
Progressive multifocal leukoencephalopathy in AIDS: favourable response to cytosine arabinoside in three patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):191 (abstract no. M.B.2037). Unique Identifier : AIDSLINE ICA7/1203791; Portegies P; Reiss P; Algra PR; Hollak CE; Prins JM; Valk J; Lange J; Department of Neurology, Academic Medical Centre, University of; Amsterdam, Amsterdam, the Netherlands; OBJECTIVE: To assess the effect of treatment with the nucleic acid analogue cytosine arabinoside (ARA-C) on progressive multifocal leukoencephalopathy (PML) in patients with AIDS. METHODS: Three consecutive AIDS patients with a presumptive clinical diagnosis of PML (based on the following criteria: relentlessly progre
Lower limb nerve conduction studies and somatosensory evoked potentials in HIV infection: two year follow-up - the Middlesex MRC cohort study.: Int Conf AIDS. 1991 Jun 16-21;7(1):191 (abstract no. M.B.2038). Unique Identifier : AIDSLINE ICA7/1203891; Connolly S; McAllister RH; Manji H; Fell M; Durrance P; Boland M; Newman SP; Fowler CJ; Weller IV; Harrison MJ; University College & Middlesex School of Medicine, London UK; OBJECTIVE: To compare the results of repeated nerve conduction studies (NCS) and somatosensory evoked potentials (SEPs) in HIV seropositive gay men with those in a well-matched seronegative comparison group. METHODS: 81 gay men from the MRC Cohort at the Middlesex Hospital have now had 4 neurological and neurophysiolo
Cognitive impairment in AIDS and asymptomatic HIV-seropositive subjects.: Int Conf AIDS. 1991 Jun 16-21;7(1):191 (abstract no. M.B.2039). Unique Identifier : AIDSLINE ICA7/1203991; Villa G; Monteleone D; Marra C; Bartoli A; Solida A; Pallavicini F; Izzi I; Istituto di Neurologia - Universita Cattolica S.C., Roma; OBJECTIVE: The present research has been undertaken as a systematic neuropsychological study of AIDS-Dementia-Complex (ADC) in order to achieve: a) quantification of the cognitive impairment related to HIV damage on CNS across the various stages of HIV infection; b) characterization of this cognitive impairment, parti
Event-related potentials (P3) in the course of HIV-infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):192 (abstract no. M.B.2040). Unique Identifier : AIDSLINE ICA7/1204091; Schroder M; Spieles S; Kubicki S; Ruf B; Grunewald T; Pohle HD; Bienzle U; Abteilung fur Klinische Neurophysiologie, Universitatsklinikum; Rudolf Virchow, Berlin, FRG; OBJECTIVE: Up-to-date subtile methods demonstrate significant changes already in early states of HIV-infection. We examined the usefulness of P3 regarding the evaluation of eventual central-nervous deficits, especially with regard to the development of the AIDS dementia complex and
Cryptococcus neoformans meningitis in AIDS: in vitro susceptibility to antifungal drugs.: Int Conf AIDS. 1991 Jun 16-21;7(1):192 (abstract no. M.B.2041). Unique Identifier : AIDSLINE ICA7/1204191; Mengoli C; Scarin M; Peracchi M; Tonin E; Berliato G; Institute of Microbiology, University of Padua, Padua, Italy; OBJECTIVE: Cryptococcal meningitis in AIDS has a devastating course and often shows a poor outcome. During the past years, several antifungal agents have been formulated and evaluated in the treatment of deep-seated fungal infections. Among these drugs polyene antibi
Serial assessments of mood & psychiatric state - changes over time & relation to neuropsychological assessment - the Middlesex MRC cohort study.: Int Conf AIDS. 1991 Jun 16-21;7(1):192 (abstract no. M.B.2042). Unique Identifier : AIDSLINE ICA7/1204291; Fell M; Newman S; Herns M; Durrance P; Manji H; Connolly S; MacAllister R; Weller I; Harrison M; University College & Middlesex School of Medicine, University of; London, UK; OBJECTIVE: To assess mood and psychiatric state of HIV +ve and ve individuals over time and examine the relationship with neuropsychology. METHODS: Measures of depression (BDI) state anxiety (STAI) and psychiatric state (CIS) and a comprehensive neuropsychological battery were administered to 125 homosexual male volun
Myelopathy in HIV infection and zidovudine therapy.: Int Conf AIDS. 1991 Jun 16-21;7(1):192 (abstract no. M.B.2043). Unique Identifier : AIDSLINE ICA7/1204391; Galgani S; Gerace C; Meccia A; Narciso P; Sette P; Tozzi V; Visco G; USL RM10: San Camillo Hosp., Rome Italy; OBJECTIVE: To evaluate the efficacy of Zidovudine (ACT) in the treatment of myelopathy in HIV infection. METHODS: 20 pts with clinical diagnosis of non compressive myelopathy were included. 18 were AIDS, 2 ARC, All pts but one had CD4 less than 200/mul; CSF and SEP studied were performed in 13 pts; NMR of brain and sp
Lack of racial, gender, and behavior-risk diversity in psychiatric research on AIDS/HIV in the United States.: Int Conf AIDS. 1991 Jun 16-21;7(1):193 (abstract no. M.B.2044). Unique Identifier : AIDSLINE ICA7/1204491; Nakajima GA; Rubin HC; Department of Psychiatry, New York University; New York, New York; USA; OBJECTIVE: To determine the demographics of subjects in psychiatric research on AIDS/ HIV in the United States . METHODS: All articles (31) with subjects who had AIDS/ HIV from the American Journal of Psychiatry (20), Hospital and Community Psychiatry (8) and Archives of General Psychia
Peripheral autonomic surface potential (PASP) as a marker of autonomic nervous system dysfunction in HIV-seropositives.: Int Conf AIDS. 1991 Jun 16-21;7(1):193 (abstract no. M.B.2046). Unique Identifier : AIDSLINE ICA7/1204691; Malessa R; Ohrmann P; Brockmeyer NH; Rimpel J; Department of Neurology, University of Essen, Germany; OBJECTIVE: To study autonomic nervous system function in HIV-infected individuals by means of a quantitative technique for recording sympathetic conduction, the peripheral autonomic surface potential (PASP). METHODS: PASP were recorded from the palm after contralateral stimulation of the median nerve at the wrist in 3
Psychiatric symptoms and neuropsychological deficits in HIV-infected patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):193 (abstract no. M.B.2047). Unique Identifier : AIDSLINE ICA7/1204791; Naber D; Perro C; Schick U; Einhaupl K; Vieler T; Binder J; Schielke E; Riedel RR; Dept. of Psychiatry, University of Munich, Germany; Psychiatric symptoms and neuropsychological deficits occur in all stages of HIV-infection, data on frequency and severity and etiology, however, are controversial. In a longitudinal study, 190 HIV positive patients (64 asym., 48 LAS, 34 ARC, 44 AIDS) and 117 HIV-negative controls underwent psychiatric exploration and
A four year decline in neuropsychological function in symptomatic HIV seropositives compared to asymptomatic seropositives and seronegative former intravenous drug users (IVDUs).: Int Conf AIDS. 1991 Jun 16-21;7(1):194 (abstract no. M.B.2048). Unique Identifier : AIDSLINE ICA7/1204891; Silberstein CH; O'Dowd MA; Friedland G; Schoenbaum E; Cochrane K; Hartel D; McKegney FP; New York University, New York, New York, USA; OBJECTIVE: To examine cognitive function prospectively over the time course of HIV-1 infection in IVDUs, from asymptomatic seropositivity through the development of ARC/AIDS, using seronegative IVDUs as a comparison group. METHODS: A prospective longitudinal study of 220 methadone maintenance clinic former IVDUs was i
Peptide T studies: neuropsychologic results.: Int Conf AIDS. 1991 Jun 16-21;7(1):194 (abstract no. M.B.2049). Unique Identifier : AIDSLINE ICA7/1204991; McGrail M; Heseltine PN; Eaton E; Parker E; Ruff M; Buzy J; Pert C; Bridge P; University of Southern California, Los Angeles, CA., USA; OBJECTIVE: To evaluate neuropsychologic measures assessed at baseline and after Phase I testing of Peptide T (D-ala-1-peptide-T-amide), a homologue of VIP and gp-120, and a gp-120 receptor blocker. METHODS: At baseline and after 12 weeks of escalating intravenous testing of Peptide T in 15 AIDS and ARC patients receiv
Intrathecal zidovudine for AIDS dementia complex.: Int Conf AIDS. 1991 Jun 16-21;7(1):194 (abstract no. M.B.2050). Unique Identifier : AIDSLINE ICA7/1205091; Routy JP; Allfgre T; Blanc AP; Rodriguez E; Kiegel P; Vialley F; Lagier E; Hopital Cezanne Aix en Provence, France; OBJECTIVE: We wanted to evaluate in an open prospective trial the toxicity and efficacy of AZT injected intrathecally (IT) in patients (pts) with AIDS dementia complex (ADC) stage III and IV, previously treated by
Do typical neuropsychological patterns exist in different HIV-positive risk groups?: Int Conf AIDS. 1991 Jun 16-21;7(1):194 (abstract no. M.B.2051). Unique Identifier : AIDSLINE ICA7/1205191; Riedel RR; Schulz-Kindermann F; Helnstaedter CH; Brackmann HH; Viehler T; Haber D; Dep. of Psychiatry, LMC, Nussbaumstr, 7, 8000 Munich 2, FRG; OBJECTIVE: In several studies especially homosexual HIV-patients were examined by neuropsychological tests. But so far we know any data are published to the question if there were a difference in mental deficits considering various cofactors (lues, cerebral toxic effect of drugs, malnutrition) of the different risk gr
Electrophysiological investigations of proximal and distal polyneuropathy in early and late HIV-infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):195 (abstract no. M.B.2052). Unique Identifier : AIDSLINE ICA7/1205291; Malessa R; Ohrmann P; Brockmeyer NH; Mertins L; Department of Neurology, University of Essen, Germany; OBJECTIVE: To describe the development and distribution of HIV-related sensorimotor polyneuropathy by electrophysiological means. METHODS: Thirty-five HIV-seropositive individuals and 20 age and height matched controls underwent the following electrophysiological programm: The distal motor latency (DML) serving as mar
Central nervous system stimulants as symptomatic treatment for AIDS dementia complex.: Int Conf AIDS. 1991 Jun 16-21;7(1):195 (abstract no. M.B.2053). Unique Identifier : AIDSLINE ICA7/1205391; Angrist B; d'Hollosy M; Satriano J; Sanfilipo M; Diamond G; Simberkoff M; Weinreb H; Department of Veterans Affairs Medical Center, New York, New; York, USA; OBJECTIVE: The present investigation is a single blind study of the neuropsychological and clinical effects of psychostimulants on patients with the AIDS Dementia Complex. METHODS: Seven patients who demonstrated significant symptoms of ADC on neuropsychological evaluation were treated with rising doses of Methylpheni
Gender differences apparent on neurological examination of HIV-infected parenteral drug users.: Int Conf AIDS. 1991 Jun 16-21;7(1):195 (abstract no. M.B.2054). Unique Identifier : AIDSLINE ICA7/1205491; Marder K; Malouf R; Dooneief G; Bell K; Tang M; Gorman J; Ehrhardt A; Mayeux R; HIV Center for Clinical and Behavioral Studies, New York State; Psychiatric Institute, Columbia University and Harlem Hospital; Center, New York, NY, USA; OBJECTIVE: HIV may differentially affect men and women, thus our objective to identify neurological manifestations of HIV in parenteral drug users (PDU) of both sexes. METHODS: Blind to HIV status, a structured neurological exam including a symptom checklist was administered to 221 subjects, 145 men (83+, 62-) and 76
Neuro-psychometric assessment and neuroimagining in asymptomatic and symptomatic HIV-1 infected subjects.: Int Conf AIDS. 1991 Jun 16-21;7(1):195 (abstract no. M.B.2055). Unique Identifier : AIDSLINE ICA7/1205591; Mancuso A; Moriconi M; Palattella A; Bianco F; Spadaro M; Tilia G; D'Offizi G; Mezzaroma I; Guerrisi R; Dept. Psychiatry, Univ. of Rome La Sapienza, Rome, Italy; OBJECTIVE: To study the prevalence of HIV-1 related neuropsychiatric disorders in a group of infected subjects by neuroimaging, neurological and psychometric assessment. METHODS: 65 HIV-1 infected subjects, 20 asymptomatic and 45 classified as ARC or AIDS were submitted to neurological examination, Wechsler-Bellevue I
Evidence for neurotoxic activity of tat from human immunodeficiency virus (HIV-1).: Int Conf AIDS. 1991 Jun 16-21;7(1):196 (abstract no. M.B.2056). Unique Identifier : AIDSLINE ICA7/1205691; Elmostafa B; Vives E; Mabrouk K; Benjouad A; Rochat H; Duval A; Hue B; Van Rletschoten J; Sabatier JM; Laboratoire de Biochimie, CNRS URA 1179, Faculte de Medecine,; Secteur Nord, Bd P. Dramard, 13326-Marseille Cedex 15, France; OBJECTIVE: To investigate the neurotoxic activity of tat from human immunodeficiency virus. METHODS: Chemically synthetic tat and tat peptide derivatives were tested for their binding to synaptosomal rat brain membranes or rat glioma and murine neuroblastoma cells. This interaction was also investigated with electroph
Prospective testing using a 2-tone auditory event-related (AEP) task shows significant slowing in HIV infected drug users.: Int Conf AIDS. 1991 Jun 16-21;7(1):196 (abstract no. M.B.2057). Unique Identifier : AIDSLINE ICA7/1205791; Chiswick A; Egan V; Brettle RP; Goodwin GM; Infectious Diseases Unit, City Hospital, Edinburgh, Scotland; OBJECTIVE: To determine whether serial testing of HIV positive drug users (DUs) using a 2-tone AEP task, and in particular the latency of the P300 (P3) component provides evidence of brain involvement by HIV. METHODS: Two hundred and sixty DUs (M:F=2:1) infected 6 - 7 years previously were recruited. One hundred and f
Neuropsychological findings in CDC group II gay men - one year follow-up of the St. Stephen's cohort study.: Int Conf AIDS. 1991 Jun 16-21;7(1):196 (abstract no. M.B.2058). Unique Identifier : AIDSLINE ICA7/1205891; Riccio M; Pugh K; Catalan J; Jadresic D; Baldweg T; Hawkins D; Charing Cross & Westminster Medical School, London, UK; OBJECTIVE: To assess the association between HIV infection and cognitive deterioration in a cohort of asymptomatic gay men. METHOD: 68 HIV seronegative (-ve) and 33 HIV seropositive (+ve) gay men were recruited from an HIV Clinic in central London when requesting an HIV Ab test to take part in a longitudinal study. Ne
Role of 99mTcHM-PAO spect in AIDS patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):196 (abstract no. M.B.2059). Unique Identifier : AIDSLINE ICA7/1205991; Baccega M; Campana M; Cesana P; Ropolo R; Soranzo ML; De Filippi PG; Servizio Medicina Nucleare Ospedaliera, Ospedale Molinette,; Torino, Italy; The aim of work was to evaluate changes of regional cerebral blood flow in AIDS patients (pts) to relate the pattern with Computed Tomography (TC) and to see if correlation exists between scintigraphic pattern and laboratory tests (T4 and T8 lymphocyte counts, whose predictive significance in disease is known). We hav
The effect of zidovudine on neuropsychological performance in patients with asymptomatic HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):197 (abstract no. M.B.2060). Unique Identifier : AIDSLINE ICA7/1206091; Grunseit A; Perdices M; Dunbar N; Craven A; Cooper DA; National Centre in HIV Epidemiology and Clincal Research,; University of New South Wales, Sydney, Australia; OBJECTIVE: To examine the effect of zidovudine (ZDV) on neuropsychological performance (NP) over 12 months in a group of asymptomatic HIV infected persons. METHOD: Subjects were 61 HIV positive patients (CDC groups II-III) enrolled in a double blind, placebo controlled ZDV trial. NP was assessed on a wide range of fun
A longitudinal study of neurocognitive changes in HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):197 (abstract no. M.B.2061). Unique Identifier : AIDSLINE ICA7/1206191; Woo S; Hunter J; Wang P; Wortzman G; Simor A; Fernandes B; Ichise M; Chemerynsky I; Mount Sinai Hospital, University of Toronto, Toronto, Ontario,; Canada; OBJECTIVES: 1) To study over 2 years the neuropsychological functioning of persons with HIV infection. 2) To design a screening measure with the capacity for early detection of neurocognitive impairment in HIV infected individuals. 3) To establish if scores on this measure may be used to infer the degree of CNS or imm
Anxiety and depression within a population suffering from AIDS: comparison with a mixed population affected by malignant hemopathy.: Int Conf AIDS. 1991 Jun 16-21;7(1):197 (abstract no. M.B.2062). Unique Identifier : AIDSLINE ICA7/1206291; Moatti M; Aubin F; Charlot V; Ades J; Hopital Louis Mourier, Service de Psychiatrie, Colombes, France; Anxiety and depressed mood are both the most frequent psychiatric symptoms described in patients with AIDS. For some authors, the anxiodepressive reaction does not differ from the reactions observed in case of mortal illness, like cancer for example; but, these statements do not rely, to the best of our knowledge, upo
Predictive role of EEG in AIDS pediatric patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):197 (abstract no. M.B.2063). Unique Identifier : AIDSLINE ICA7/1206391; Piazza F; Caselli D; Comelli D; Maccabruni A; Veggiotti P; Viri M; Cattedre di Neuropsichiatria Infantile Fondazione C. Mondino; OBJECTIVE: This study was designed to investigate the significance of EEG in predicting the clinical course of HIV infection in pediatric age and to study the usefulness of EEG in revealing initial central nervous system dysfunctions. METHODS: 18 children HIV seropositive have been studied, grouped according to Atlant
Neuropsychiatric impairment in HIV+ persons.: Int Conf AIDS. 1991 Jun 16-21;7(1):198 (abstract no. M.B.2064). Unique Identifier : AIDSLINE ICA7/1206491; McManis SE; Brown GR; Zachary R; Kendall S; Rundell JR; Wilford Hall Medical Center, San Antonio, TX, USA; OBJECTIVE: To assess neuropsychiatric impairment (NPI), as measured by Rey-Osterreith Complex Figure Copy (ROC) and Memory (ROM) scores, in predominantly asymptomatic HIV+ persons compared to matched seronegative controls. METHODS: 97 HIV+ subjects (63 men, 34 women/85% asymptomatic, 8% ARC, 5% AIDS) underwent standar
Psychiatric distress, cortisol, and activated T lymphocytes in HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):198 (abstract no. M.B.2065). Unique Identifier : AIDSLINE ICA7/1206591; Kertzner R; Gorman J; Williams J; Cooper T; Reddy M; Ehrhardt AA; HIV Center for Clinical and Behavioral Studies at the New York; State Psychiatric Institute and Columbia University, New York,; NY, USA; OBJECTIVE: To examine the relationship between depression and anxiety, cortisol levels, and T lymphocyte activation. METHODS: HIV-infected gay men (N=112, Time One; N=104, Time Two; N=65, Time Three) and HIV seronegative gay male controls (N=75, Time One; N=47, Time Two; N=14, Time Three) were assessed for Hamilton De
Accelerated brain atrophy and parenchymal change in patients with AIDS.: Int Conf AIDS. 1991 Jun 16-21;7(1):198 (abstract no. M.B.2066). Unique Identifier : AIDSLINE ICA7/1206691; Hesselink J; Tien R; Spoto G; Jernigan T; Chandler J; Grant I; University of California, San Diego, Naval Hospital, and VA; Medical Center, San Diego, CA, USA; OBJECTIVE: As part of our HIV Neurobehavioral Research Center (HNRC), imaging studies were performed to determine the effects of HIV infection on the brain at various stages of the disease. METHODS: MR imaging was performed on 159 subjects, including 88 in a combined group of CDC classes 2, 3, and 4-C2, 36 CDC Class 4
Beta-endorphin, ACTH and cortisol in CSF (B endl) and plasma (Bendp) of HIV infected patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):198 (abstract no. M.B.2067). Unique Identifier : AIDSLINE ICA7/1206791; Spinazzola F; Barletta C; Demartino G; Natili S; Noto P; Tossini G; Visco G; USL RM/10 L. Spallanzani Hospital for Infectious Diseases Rome,; Italy; OBJECTIVE: Our aim has been to correlate CSF and plasma levels of beta endorphin, ACTH, cortisol in 60 HIV patients, at different stages of infection, who had never been drug addicts, with CD4 cell levels. METHODS: 60 HIV patients and 20 controls underwent one or more lumbar punctures and blood samples. Beta endorphin
The safety and efficacy of mexiletine in HIV-associated painful peripheral neuropathy (PPN).: Int Conf AIDS. 1991 Jun 16-21;7(1):199 (abstract no. M.B.2068). Unique Identifier : AIDSLINE ICA7/1206891; Kent GP; Ganer A; Deresinski SC; Santa Clara Valley Medical Center, San Jose, California, USA; OBJECTIVE: To evaluate the safety and analgesic efficacy of mexiletine (Mx) in HIV-associated PPN. METHODS: Adult HIV-infected patients with PPN having moderate to severe pain and abnormal nerve conduction studies or abnormal neurologic examination were randomized to receive either Mx (10 mg/kg/d) or placebo in a doub
CMV encephalitis in AIDS patients: a clinico-pathological correlation.: Int Conf AIDS. 1991 Jun 16-21;7(1):199 (abstract no. M.B.2069). Unique Identifier : AIDSLINE ICA7/1206991; Castagna A; Vago L; Accordini A; Moscatelli G; Costanzi G; Lazzarin A; Clinic of Infectious Diseases, V University of Milan, Milan,; Italy; OBJECTIVE: To evaluate the frequency and clinical features of CMV encephalitis. METHODS: Brain tissue from 168 AIDS patients were examined at autopsy to assess the presence of CMV infection. When available, clinical data with particular attention to neurologic s
Development of early subclinical alterations in peripheral and central auditory pathways in HIV-infection. An electrophysiological follow up study.: Int Conf AIDS. 1991 Jun 16-21;7(1):199 (abstract no. M.B.2070). Unique Identifier : AIDSLINE ICA7/1207091; Malessa R; Rose N; Brockmeyer N; Mertins L; Schwendemann G; Department of Neurology, University of Essen, Germany; OBJECTIVE: To study the development and distribution of alterations in peripheral and central auditory pathways in HIV-infection by electrophysiological means. METHODS: Twenty-nine HIV-seropositive neurological asymptomatic homosexuals and 27 seronegative controls were tested with auditory evoked potentials (AEP) (cli
Neurological effect of continuous versus intermittent zidovudine therapy in ARC and AIDS patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):199 (abstract no. M.B.2071). Unique Identifier : AIDSLINE ICA7/1207191; Enzensberger W; Madlener J; Herdt P; Staszewski S; Helm EB; Fischer PA; Dept. of Neurology, University Hospital Frankfurt/M, Germany; OBJECTIVE: While the efficacy of zidovudine therapy on the course of the HIV disease is well known, neurological consequences are less clear. Our prospective longitudinal therapy study compares two different regimens (500mg zidovudine per day given continuously vs. 1000mg per day given 4 weeks, alternating with 4 week
Distal symmetrical painful neuropathy (DSPN) in course of HIV-infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):200 (abstract no. M.B.2072). Unique Identifier : AIDSLINE ICA7/1207291; Rizzuto N; Cavallaro T; Bonetti B; Ferrari S; Monaco S; Morbin M; Danzi MC; Di Perri G; Cruciani M; Bassetti D; Institute of Neurology, University of Verone, Verone, Italy; DSPN is the commonest complication of Peripheral Nervous System (PNS) involvement in course of AIDS, and its pathogenesis is still controversial. -Clinical, electrophysiological and pathologic evaluation of nerve biopsy was performed in 14 patients with HIV infection and DSPN without other causes of known neuropathy.
BK-type polyomavirus meningoencephalitis in a patient with AIDS.: Int Conf AIDS. 1991 Jun 16-21;7(1):200 (abstract no. M.B.2073). Unique Identifier : AIDSLINE ICA7/1207391; Gluck T; Lohler J; Dorries K; Vallbracht A; Medizinische Univesitatsklinik Tubingen, Germany; INTRODUCTION: BK-virus infections are occasionally seen in immunocompromised patients, most often as urinary tract infections in renal graft recipients. We report the first case of BK-virus meningoencephalitis in a patient with AIDS. CASE: The 29 year old man had suffered from hemophilia A. HIV-Infection was diagnosed
Cognitive and behavioral effects of AZT-IL2.: Int Conf AIDS. 1991 Jun 16-21;7(1):200 (abstract no. M.B.2074). Unique Identifier : AIDSLINE ICA7/1207491; Ollo C; Bridge TP; Kenagy G; Govoni L; NIMH and LCS, 9000 Rockville Pike, Bethesda MD; OBJECTIVE: To evaluate changes in cognition and affect resulting from acute (Phase I) IL-2 treatment in HIV+ patients stabilized on AZT . METHODS: Behavioral ratings and neuropsychological tests were ad
Rate of CD4 decline and neuropsychological performance in HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):200 (abstract no. M.B.2075). Unique Identifier : AIDSLINE ICA7/1207591; Bornstein R; Nasrallah H; Para M; Fass R; Whitacre C; Rice R Jr; Ohio State University, Columbus, Ohio, USA; OBJECTIVE: The study examined the relationship between rate of CD4 cell loss and performance on a battery of neuropsychological tests. It was hypothesized that rate of cell loss would be inversely related to test performance. METHODS: A neuropsychological test battery was administered including Selective Reminiding Te
Slowed reaction time in asymptomatic HIV-positive patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):201 (abstract no. M.B.2076). Unique Identifier : AIDSLINE ICA7/1207691; Karlsen NR; Reinvang I; Fr:oland SS; The National Hospital, Oslo, Norway; OBJECTIVE: To determine if measures of reaction time show indication of subtle performance deficit in asymptomatic HIV-positives. METHODS: 19 asymptomatic HIV-infected patients (CDC II/III) and 33 HIV-negative controls were tested on visuomotor coordination and speed of reaction. The HIV-positive group consisted of 1
Electroencephalographic monitoring of zidovudine effectiveness.: Int Conf AIDS. 1991 Jun 16-21;7(1):201 (abstract no. M.B.2077). Unique Identifier : AIDSLINE ICA7/1207791; Parisi A; Strosselli M; Caccamo F; Maserati R; Dept of Infectious Diseases - I.R.C.C.S. Policlinico San Matteo,; University of Pavia, Italy; OBJECTIVE: The Authors analyze, by an electroencephalographic monitoring, two groups of HIV seropositive patients, one group receiving a therapy with Zidovudine and a control group, in order to assess the effectiveness of the drug in the treatment of AIDS-encephalopathy. METHODS: A computerized electroencephalographic
Immunohistochemical and ultrastructural observations on the capillary walls of AIDS brain cortex.: Int Conf AIDS. 1991 Jun 16-21;7(1):201 (abstract no. M.B.2078). Unique Identifier : AIDSLINE ICA7/1207891; Malchiodi Albedi F; Taruscio D; Beltrano MP; Pauluzzi S; Costanzi G; Vago L; Donelli G; Laboratorio di Ultrastrutture, Istituto Superiore di Sanita,; Rome, Italy; In a previous study on the immunohistochemical modification of capillary walls in AIDS brain cortex 1), an increase in laminin content was found in affected basement membranes. In this report, we have considered the expression of various types of collagen (I, III, IV and VI), as well as the ultrastructural appearance
Effects of AZT on CNS impairment in pediatric age.: Int Conf AIDS. 1991 Jun 16-21;7(1):201 (abstract no. M.B.2079). Unique Identifier : AIDSLINE ICA7/1207991; Musetti L; Corsi A; Marchisio P; Massironi E; Saccani M; Tornaghi R; Istituto di Neuropsichiatria Infantile-Universita di Milano; Milano Italy; OBJECTIVE: To assess the effect of zidovudine ( AZT ) on neurologic symptomatology in congenitally HIV infected (HIV+) children. METHODS: Prospective study from birth of 3 HIV+ children who subsequently developed an encephalopathy. Neurologic evaluation
The involvement of peripheral nervous system as a differentiating element in the various stages of HIV disease.: Int Conf AIDS. 1991 Jun 16-21;7(1):202 (abstract no. M.B.2080). Unique Identifier : AIDSLINE ICA7/1208091; Parisi L; Calandriello E; Terracciano M; Valente G; Pinter E; Spadaro M; Morocutti C; Institute of Immunology - University La Sapienza Rome, Italy; OBJECTIVE: Electrophysiological study of Peripheral Nervous System (PNS) in HIV+ patients by transversal evaluation, for a possible individuation of different infection stages. METHODS: Forty patients in various stages of disease (HIV+ asymptomatic, LAS, ARC, AIDS) were examined. An electromyographycal study with anal
Electrophysiological motor testing, MRI findings and clinical course in AIDS-patients with dementia.: Int Conf AIDS. 1991 Jun 16-21;7(1):202 (abstract no. M.B.2081). Unique Identifier : AIDSLINE ICA7/1208191; Hefter H; Arendt G; Jablonowski H; Freund HJ; Department of Neurology and Medicine, University of Dusseldorf; OBJECTIVE, METHODS: In 33 AIDS-patients-CDC-stages IV A-E with clinical signs of dementia in clinical (general mental slowing, memory dysfunction, affective nivellation) and psychometrical testing (multiple choice vocabulary test-form b 10 raw points than standard progressive matri
CMV encephalitis: clinical, biological, virological and immunopathological correlation in 22 patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):202 (abstract no. M.B.2082). Unique Identifier : AIDSLINE ICA7/1208291; Chieze F; DeSa M; Katlama C; Henin D; Vazeux R; Hauw JJ; Service Maladies Infectieuses et Parasitaires, Paris, France; OBJECTIVE: Because CMV encephalitis appears to be underdiagnosed this study tries to evaluate the prevalence of CNS-CMV infection and to precise its clinical, biological and virological manifestations. METHODS: Retrospective autopsic study on 58 AIDS-patients fr
CSF markers for monitoring the AIDS dementia complex severity.: Int Conf AIDS. 1991 Jun 16-21;7(1):202 (abstract no. M.B.2083). Unique Identifier : AIDSLINE ICA7/1208391; Mastroianni CM; Liuzzi GM; Paoletti F; Jirillo E; Vullo V; Delia S; Ricco P; University La Sapienza, Rome, Italy; OBJECTIVE: To investigate the usefulness of CSF markers for predicting the degree of neurological impairment during AIDS dementia complex (ADC). METHODS: CSF samples collected from 19 ADC patients and from 8 HIV+ without CNS involvement were assessed for HIV-Ag, beta2M, TNF, IgG co
Psychiatric disorders, drug use, and HIV disease in 100 HIV-infected intravenous drug-users (IVDUs).: Int Conf AIDS. 1991 Jun 16-21;7(1):203 (abstract no. M.B.2084). Unique Identifier : AIDSLINE ICA7/1208491; Batki SL; Ferrando S; London J; Pattillo J; Ryan C; Abbott C; Hartwig R; Goosby E; University of California, San Francisco, CA USA; OBJECTIVE: To describe the prevalence of psychiatric disorders and their relationship to drug use and HIV status in HIV-infected IVDUs in methadone maintenance treatment (MMT). METHOD: The hospital records of 100 HIV-infected patients in MMT at San Francisco General Hospital were examined for psychiatric disorders, HI
Evaluation of cognitive functions in HIV-positive, intravenous drug users.: Int Conf AIDS. 1991 Jun 16-21;7(1):203 (abstract no. M.B.2085). Unique Identifier : AIDSLINE ICA7/1208591; Grassi MP; Clerici F; Perin C; Mezzenzana MA; Dedivitis GF; Cargnel A; Mangoni A; Department of Neurology, L. Sacco Hospital, Milan, Italy; OBJECTIVE: Evaluate the existence of cognitive deficits, as early signs of CNS involvement, and their evolution in HIV-positive (HIV+) intravenous (iv) drug users without clinical signs of neurological impairment. METHODS: Fifty-three HIV+ iv drug users, 19 HIV-negative (HIV-) iv drug users, and 26 healthy subjects we
Interest of neuropsychologic and electrophysiologic evaluation in HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):203 (abstract no. M.B.2086). Unique Identifier : AIDSLINE ICA7/1208691; Moulin T; Vanhems P; Schoenfelder F; Valet B; Monnier G; Rousselot JP; Chopard JL; Drobacheff C; De Wazieres B; Bertrand MA; Besancon University Hospital - 25030 Cedex Besancon - France; To study the frequency, presentation and evolution of the earliest neurologic manifestations in a group of 145 HIV positive patients enrolled prospectively since 1987. The patients (P) were screened with a standard protocol: neurologic exams, neuropsychologic (NP) tests (progressive matrices PM38 or PM47, Weschler mem
Usefulness of magnetic resonance imaging in febrile HIV-infected patients presenting central nervous system manifestations.: Int Conf AIDS. 1991 Jun 16-21;7(1):203 (abstract no. M.B.2087). Unique Identifier : AIDSLINE ICA7/1208791; Arvieux C; Michelet C; Heautot JF; Cartier F; Clinique des Maladies Infectieuses, Hopital Pontchaillou, CHR de; Rennes, France; OBJECTIVE: To appreciate the usefulness, clinical and therapeutic impact of Magnetic Resonance Imaging (MRI) compared with Computed Tomography (CT) in febrile patients with progressive HIV-infection and Central Nervous System (CNS) manifestations. METHODS: In order to appreciate the utility and the clinical or therape
Neuropsychological study of the AIDS dementia complex: a preliminary report.: Int Conf AIDS. 1991 Jun 16-21;7(1):204 (abstract no. M.B.2088). Unique Identifier : AIDSLINE ICA7/1208891; Lacorte TM; Ocana MI; Martinez-Vazquez JM; Vall d'Hebron General Hospital, Barcelona, Spain; OBJECTIVE: A progressive dementing illness is among the most common and devastating manifestations of infection by the retrovirus that causes AIDS. In order to more precisely define the character of the cognitive, motor and behavioral changes associated with this dementia , we have
Neuropsychological status of men with haemophilia and HIV infection: a controlled investigation - one year follow-up.: Int Conf AIDS. 1991 Jun 16-21;7(1):204 (abstract no. M.B.2089). Unique Identifier : AIDSLINE ICA7/1208991; Klimes I; Elcombe S; Hodges S; Catalan J; Day A; Garrod A; Bond A; Rizza C; Department of Clinical Psychology, Warneford Hospital, Oxford, UK; OBJECTIVE: To establish prevalence and possible development of neuropsychological problems in HIV+ men with haemophilia as part of a 3 year prospective study. Twelve month follow-up data is given. METHODS: Twenty-two HIV+ and 29 HIV- men with haemophilia were assessed on measures of pre-morbid IQ (Nart); verbal skills
Toxoplasmic encephalitis in 73 AIDS patients (Bordeaux, France, 1985-1989).: Int Conf AIDS. 1991 Jun 16-21;7(1):204 (abstract no. M.B.2090). Unique Identifier : AIDSLINE ICA7/1209091; Ragnaud JM; Beylot J; Lacut JY; Leng B; Aubertin J; Salamon R; Regional University Hospital, Bordeaux, France; OBJECTIVE: To describe the main epidemiological, clinical, radiological features of Toxoplasmosis encephalitis (TE) and its outcome among AIDS patient. METHODS: Diagnosis of TE was presumptive, using clinical and radiological criterias. RESULTS: TE was diagnosed in 73 of the 428 AIDS patients followed in the Bordeaux
Sulfadiazine crystalluria and acute renal failure in AIDS patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):204 (abstract no. M.B.2091). Unique Identifier : AIDSLINE ICA7/1209191; Patey O; Dellion S; Halioua B; Lafaix C; Departement des Maladies Infectieuses et Tropicales, Villeneuve; St Georges, France; OBJECTIVE: To aware physicians of the potentiel danger of sulfonamide induced injury to the urinary tract. CASE REPORTS: We observed two cases of sulfadiazine crystalluria with transient renal failure in AIDS patients treated for toxoplasmic encephalitis (T.E.) with pyrimethamine (100 mg/day) and sulfadiazine (5 gr./d
Seven cases of primary cerebral lymphoma in AIDS patients: prominence of tumoral necrosis.: Int Conf AIDS. 1991 Jun 16-21;7(1):205 (abstract no. M.B.2092). Unique Identifier : AIDSLINE ICA7/1209291; Vital C; Merlio JP; Rivel J; Vital A; Gautris P; Beylot M; Delors B; Department of Neuropathology, Bordeaux II University, France; Seven cases of primary cerebral lymphoma (PCL) were studied on fragments from biopsy (3 cases), autopsy (3 cases) and both (1 case). These 4 latter cases were selected from a larger group of 39 brains from patients with AIDS autopsied since 1985, and were multicentric PCL. The 7 PCL were immunoblastic-plasmocytoid hig
Specific IgA and IgE in toxoplasmic encephalitis of AIDS patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):205 (abstract no. M.B.2093). Unique Identifier : AIDSLINE ICA7/1209391; Orlando G; Foudrinier F; Adriani BA; Aubert D; Marx C; Mougeot G; Danis M; Cargnel A; Pinon JM; II Divisione malattie Infettive, Osp L. Sacco, Milano, Italy; OBJECTIVE: Diagnosis of toxoplasmic encephalitis in AIDS patients relies mostly on epidemiological, radiological and therapeutic arguments while immunological findings of specific IgG and IgM are disappointing. IgA and IgE specific antibodies on serum and CSF samples of toxoplasmic encephalitis in AIDS patients are he
On the specificity of conjoined activities of major cerebral hemispheres in the HIV infected (based on electroencephalography data).: Int Conf AIDS. 1991 Jun 16-21;7(1):205 (abstract no. M.B.2094). Unique Identifier : AIDSLINE ICA7/1209491; Makarova GV; Central Research Insititue of Epidemiology of the USSR Ministry; of Public Health, Moscow, USSR; In 50 HIV infected persons with lymphoadenopathy several types of CNS reactions were discovered which determine the degree of adaptive brain reactions formation. The adaptive reactions were evaluated using the electroencephalographic method. The general level of brain functional state and the parameters determining in
Cognitive performance in HIV-infected homosexual/bisexual men: cross-sectional and longitudinal study.: Int Conf AIDS. 1991 Jun 16-21;7(1):205 (abstract no. M.B.2095). Unique Identifier : AIDSLINE ICA7/1209591; Mauri M; Sinforiani E; Muratori S; Zerboni R; Alessi E; Bono G; Neuro-AIDS Unit, IRCCS C. Mondino/University of Pavia, Italy; OBJECTIVE: The neuropsychological performance of seropositive asymptomatic homosexual/bisexual men without history of alcohol/drug abuse was investigated in order to detect eventual subclinical cognitive changes predictive of early CNS involvement from HIV. METHODS: N.45 HIV-infected pts. (mean age 36,7 +/- 11,7 yrs,
Adenosine deaminase activity in sera of AIDS patients with cerebral lesion.: Int Conf AIDS. 1991 Jun 16-21;7(1):206 (abstract no. M.B.2096). Unique Identifier : AIDSLINE ICA7/1209691; Veccia S; Giacometti A; Drenaggi D; Fiorentini A; Marsili G; Scalise G; Institute of Infectious Diseases - University of Ancona, Italy; OBJECTIVE: The aim of our study was to value if adenosine deaminase activity may be useful as an early serologic marker of cerebral lesion in AIDS patients. METHODS: Adenosine deaminase activity was estimated in sera of three groups of subjects: A) 26 AIDS patients with cerebral lesion proved by computerized tomograph
Oral AZT therapy and its effect on neuro-developmental functioning.: Int Conf AIDS. 1991 Jun 16-21;7(1):206 (abstract no. M.B.2097). Unique Identifier : AIDSLINE ICA7/1209791; Nozyce M; Weisberger M; Arpadi S; Lambert G; Wiznia A; Bronx Lebanon Hospital Center, Albert Einstein College of; Medicine, Bronx, New York; OBJECTIVE: To assess whether 6 months of oral AZT therapy will improve the neuro-developmental functioning of symptomatic HIV infected children. METHODS: Nineteen symptomatic HIV infected children ranging in age from 2 to 9 years of age were placed on
Human immunodeficiency virus related headache.: Int Conf AIDS. 1991 Jun 16-21;7(1):206 (abstract no. M.B.2098). Unique Identifier : AIDSLINE ICA7/1209891; Brew BJ; Miller J; Centre for Immunology, St. Vincent's Hospital, Sydney, Australia; OBJECTIVES: Headache may be a prominent feature in HIV infected patients and is usually due to an opportunistic infection, neoplasm or aseptic meningitis. However, we have observed several patients who had no such causes found. We, therefore, studied the prevalence and clinical features of such headache. METHODS: Pati
Psychiatric diagnoses in HIV seropositive and AIDS patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):206 (abstract no. M.B.2099). Unique Identifier : AIDSLINE ICA7/1209991; Pasquale P; Nisita C; Galli L; Cassano GB; Scasso A; Stampacchia G; Della Santa M; Institute of Psychiatric Clinic, University of Pisa (Italy); OBJECTIVE: The aim of this study was to investigate the incidence and the diagnosis of psychiatric disorders in a sample of seropositive patients and to correlate them with the HIV infection stage and with the main risk behaviours. METHODS: Among the patients coming to the AIDS Center of Pisa for diagnostic evaluation
Case control study of psychosocial status in HIV seropositive women.: Int Conf AIDS. 1991 Jun 16-21;7(1):207 (abstract no. M.B.2100). Unique Identifier : AIDSLINE ICA7/1210091; Mellers J; Smith JR; Harris JR; King MB; Academic Department of Psychiatry, Royal Free Hospital, London,; UK; OBJECTIVE: This study was designed to assess the prevalence of psychiatric morbidity in a group of HIV seropositive women. METHODS: Using the Clinical Interview Schedule, psychiatric morbidity was assessed in 25 HIV seropositive women and 14 HIV seronegative controls, randomly selected from women enrolled in a colposc
The WHO cross-cultural study on the neuropsychiatric aspects of HIV-1 infection: A progress report.: Int Conf AIDS. 1991 Jun 16-21;7(1):207 (abstract no. M.B.2101). Unique Identifier : AIDSLINE ICA7/1210191; Maj M; Janssen R; Satz P; Zaudig M; Starace F; Bhiron S; Bing E; Luabeya M; Ndetei D; Riedel R; et al; WHO, Geneva, Switzerland; OBJECTIVE: The study aims to explore the nature and prevalence of HIV-1-associated neurological and psychiatric disorders and neuropsychological abnormalities in persons living in different geographic and socio-cultural contexts. METHODS: The study is being performed in Bangkok, Thai
HIV isolation and p24-antigen detection from blood and cerebrospinal fluid of patients in different clinical conditions.: Int Conf AIDS. 1991 Jun 16-21;7(1):207 (abstract no. M.B.2102). Unique Identifier : AIDSLINE ICA7/1210291; Roscioli B; Mascioli M; Andrian P; D'Agaro P; Dal Molin G; Comar M; Faruzzo A; Majori L; Department of Infectious Diseases, Trieste; OBJECTIVE: To relate HIV-1 isolation efficiency and p24 antigen detection in paired specimens of blood and cerebrospinal fluid (CSF) with patients clinical staging according to Walter-Reed (W.R.) criteria. METHODS: 40 paired specimens of CSF and blood from seropositive subjects were examined for HIV virus isolation in
Cryptococcal meningitis em Brazilian AIDS patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):207 (abstract no. M.B.2103). Unique Identifier : AIDSLINE ICA7/1210391; Lima JN; Souza-Dias M; Trabasso P; Aoki F; Abreu W; Oliveira A; Pedro R; UNICAMP Medical School, Campinas, Sao Paulo, Brasil; Between January 1989 and December 1990, 24 patients with Cryptococcal Meningitis were seen at a 400 bed acute care UNIVERSITY HOSPITAL in CAMPINAS, a 1,2 million inhabitants town in southeastern Brazil . Twenty patients had serologic evidence of HIV infection. They represented 9% of
Prevalence of psychiatric and psychosocial complications among HIV infected patients in the Netherlands.: Int Conf AIDS. 1991 Jun 16-21;7(1):208 (abstract no. M.B.2104). Unique Identifier : AIDSLINE ICA7/1210491; Buuren HJ; Lunter CH; van den Boom FM; Storosum JG; Sno HN; Netherlands Institute of Mental Health, Utrecht, The Netherlands; OBJECTIVES: Longitudinal study regarding prevalence and incidence of psychiatric, psychological and social complications of HIV infection, and needs assessment, to improve quality of care. METHODS: Outpatients of 5 major (university) clinics are asked to participate in this study. Instruments used, a.o.: DIS-III-A, Se
Two year follow-up psychiatric disorder in HIV.: Int Conf AIDS. 1991 Jun 16-21;7(1):208 (abstract no. M.B.2105). Unique Identifier : AIDSLINE ICA7/1210591; Atkinson H; Patterson T; Summers J; Brown S; Gutierrez R; Heaton R; Grant I; University of California San Diego, San Diego, CA, USA; OBJECTIVE: To determine the two year incidence of psychiatric disorder and its clinical correlates in HIV+ homosexual men and matched HIV - controls. METHODS: HIV+ (N = 109); CDC IV = 52 and CDC II-III = 57) and HIV- (N = 34) ambulatory homosexual men in a longitudinal cohort study were examined at baseline, and at 12
Neuropsychiatric complications of interferon-alpha treated patients with histories of affective symptomatology or substance use.: Int Conf AIDS. 1991 Jun 16-21;7(1):208 (abstract no. M.B.2106). Unique Identifier : AIDSLINE ICA7/1210691; Govoni LA; Davey V; Ropka ME; Demitrak MA; Lane HC; Clinical Center, NIH Bethesda, MD, USA; OBJECTIVES: To determine if HIV-infected patients with a history of affective symptoms (prior depressive symptoms, anxiety, or mood lability) or substance use (daily alcohol use, recreational drug use, alcoholism, or drug addition) are at greater risk for developing behavioral, affective, or cognitive neuropsychiatric
Symptomatological aspects of anxiety and depression in a cohort of HIV-positive patients and their relationship with the phase of infection and the belonging to a group at risk.: Int Conf AIDS. 1991 Jun 16-21;7(1):208 (abstract no. M.B.2107). Unique Identifier : AIDSLINE ICA7/1210791; Nisita C; Perretta P; Galli L; Cassano GB; Scasso A; Nuccorini A; Della Santa M; Institute of Psychiatric, University of Pisa (Italy); OBJECTIVE: Data from Anxiety and Depression items of specific rating scales have been analyzed and compared to explore the relevance of such components in the phases of infection and in the group at risk. METHODS: Seventy patients were included in the study and evaluated by the following rating scales: HAM-A HRSD, BPR
Cerebrospinal fluid levels of fibronectin in AIDS patients with central nervous system disorders.: Int Conf AIDS. 1991 Jun 16-21;7(1):209 (abstract no. M.B.2108). Unique Identifier : AIDSLINE ICA7/1210891; Zeroli C; Issi M; Ferraro G; Fiori GP; Torre D; Division of Infectious Diseases Regional Hospital, Varese, Italy; OBJECTIVE: To evaluate levels of fibronectin (FN) in cerbrospinal fluid (CSF) from AIDS patients with central nervous system (CNS) disorders. METHODS: CSF samples were collected from 36 patients with CNS disorders (AIDS dementia complex, leucoencephalopathy, opportunistic infection
Clinical utility of head CT scan (HCTs) in HIV/AIDS patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):209 (abstract no. M.B.2109). Unique Identifier : AIDSLINE ICA7/1210991; Ferro S; Fanning M; Toronto General Hospital, University of Toronto, Toronto, Canada; OBJECTIVE: To define the role of HCTs in the clinical management of the different neurologic conditions associated with HIV/AIDS. METHODS: Retrospective review of HCTs from 1984-1990. RESULTS: HCTs (n=153) were done in 92 patients (76% AIDS, 24% non-AIDS). At first HCTs, 59% were on
Improvement in psychological function in HIV+ IVDU population receiving outpatient psychiatric care.: Int Conf AIDS. 1991 Jun 16-21;7(1):209 (abstract no. M.B.2110). Unique Identifier : AIDSLINE ICA7/1211091; O'Dowd MA; McKegney FP; Albert Einstein College of Medicine, Montefiore Medical Center,; Bronx, New York, USA; OBJECTIVE: To assess changes in psychological distress in predominantly minority substance abusing patients after a year of care in an AIDS related psychiatric clinic. METHODS: 38 patients referred for psychiatric treatment due to HIV related stress were given a survey of past and present suicidal ideation/behaviors a
Relationship of HIV serostatus and education with neuropsychological impairment in asymptomatic men.: Int Conf AIDS. 1991 Jun 16-21;7(1):209 (abstract no. M.B.2111). Unique Identifier : AIDSLINE ICA7/1211191; Visscher BR; Morgenstern H; Miller EN; Satz P; Jin S; Jacobson L; Selnes O; Becker J; Cohen B; Univ of California, Los Angeles, CA, USA; OBJECTIVE: To investigate the separate and combined effects of HIV serostatus and education on neuropsychological (NP) impairment among asymptomatic homosexual men. METHODS: 1741 asymptomatic homosexual male participants in the Multicenter AIDS Cohort Study (MACS) were given a battery of NP tests that included Digit S
Cognitive deficits and ocular microangiopathic syndrome (OMS) in AIDS.: Int Conf AIDS. 1991 Jun 16-21;7(1):210 (abstract no. M.B.2112). Unique Identifier : AIDSLINE ICA7/1211291; Perro C; Geier S; Naber D; Klauss V; Binder J; Riedel R; Dept. of Psychiatry University of Munich, Germany; OBJECTIVE: Does a correlation between OMS and cognitive deficits in HIV-infected patients and AIDS exist? METHOD: 33 HIV-positive patients (21 with AIDS) underwent a neuropsychological (AVLT, trail making B) and opthalmological, including funduscopical examination. Criteria for OMS were: Cotton-Wool leasions, bleeding
Acute meningitis during HIV seroconversion: Int Conf AIDS. 1991 Jun 16-21;7(1):210 (abstract no. M.B.2113). Unique Identifier : AIDSLINE ICA7/1211391; Enzensberger R; Enzensberger W; Madlener J; Fischer PA; Doerr HW; Dept. of Virology, University of Frankfurt/M, Germany; OBJECTIVE: A Columbian female prostitute came to the out-patient department suffering from severe headache, fever, and malaise. We describe the clinical and serological evolution of the patient, who became HIV positive during her hospital stay. METHODS: The patient was submitted to complete neurological examination in
HIV seroprevalence and syphilis infection in psychiatric inpatients.: Int Conf AIDS. 1991 Jun 16-21;7(1):210 (abstract no. M.B.2114). Unique Identifier : AIDSLINE ICA7/1211491; Sacks M; Dermatis H; Looser-Ott S; Cornell University Medical College, NYC, NY, USA; OBJECTIVE: To determine whether HIV seropositivity (HIV+) is associated with a positive serologic test for syphilis (STS+) in psychiatric inpatients. METHODS: Consecutive admissions to an acute psychiatric hospital in NYC were tested for HIV in an unlinked design using waste bloods. Risk factors were obtained from the
Peripheral neuropathy in AIDS: Possible role of AZT treatment?: Int Conf AIDS. 1991 Jun 16-21;7(1):210 (abstract no. M.B.2115). Unique Identifier : AIDSLINE ICA7/1211591; Arrigo M; Zandrini C; Bollani E; Brustia R; Parisi A; University of Pavia, Pavia, Italy; OBJECTIVE: To demonstrate presence/incidence of clinical/subclinical peripheral neuropathy in AIDS patients after treatment with AZT . METHODS: 15 AIDS patients (CDC group IVC), 3 females and 12 males,
Predictive value of a single episode of Herpes zoster for HIV-1 infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):211 (abstract no. M.B.2116). Unique Identifier : AIDSLINE ICA7/1211691; Hailu K; Yigeremu A; Bekure D; Armed Forces General Hospital, Addis Ababa, Ethiopia; OBJECTIVE: To determine the predictive value of a single episode of Herpes Zoster for HIV-1 infection. METHODS: All 43 (M - 36: F-7) consecutively seen patients, at the Armed Forces General Hospital, from Sept. 1989 - Sept. 1990, with a single episode of Herpes Zoster were evaluated for the presence of both major and
A cross sectional study of psychiatric diagnoses (PD) in inner city patients infected with HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):211 (abstract no. M.B.2117). Unique Identifier : AIDSLINE ICA7/1211791; Lynch PE; Beckett A; Magocsi S; Steger KA; Craven DE; AIDS Program, Boston City Hospital, Boston, MA, USA; OBJECTIVE: To assess the prevalence of PD in HIV-1 infected persons evaluated at an inner-city hospital clinic. METHODS: Twenty consecutive patients with HIV-1 infection were evaluated by clinical psychiatric interview, symptom checklist (SCL-90-R) and neuropsychological test battery. RESULTS: Subjects were 85% male,
Diagnostic and prognostic value of neurologic clinical and electrophysiological findings in asymptomatic HIV-1 subjects.: Int Conf AIDS. 1991 Jun 16-21;7(1):211 (abstract no. M.B.2118). Unique Identifier : AIDSLINE ICA7/1211891; Giuseppina T; Spadaro M; Massara MC; Giampiero S; Valle E; Pinter E; Mezzaroma I; Aiuti F; Morocutti C; Institute of Mental and Nervous Diseases, University of Rome,; Rome, Italy; OBJECTIVE: To determine the prevalence of clinical signs of nervous system involvement in HIV-1 asymptomatic subjects and evaluate their prognostic significance. METHODS: 32 HIV-1 asymptomatic subjects were enrolled in a clinical and paraclinical (electrophysiological, neuropsychological and neuroradiological) longitu
A follow-up study in HIV positive schizophrenic patients treated with antipsychotic drugs.: Int Conf AIDS. 1991 Jun 16-21;7(1):211 (abstract no. M.B.2119). Unique Identifier : AIDSLINE ICA7/1211991; Altamura CA; Mauri MC; Coppola MT; Gianetti S; Ferrara A; Department of Clinical Psychiatry - University of Milan, Milan,; Italy; 25 HIV positive schizophrenic patients diagnosed according to DSM III-R, treated with different neuroleptic drugs at flexible dosages, were studied during 4 weeks hospitalization phase and a 4 months follow-up period. Psychopathological picture, extrapyramidal and anticholinergic side-effects were evaluated using resp
Peripheral nervous system involvement in AIDS and HIV patients: A comparative neurophysiologic and morphologic study.: Int Conf AIDS. 1991 Jun 16-21;7(1):212 (abstract no. M.B.2120). Unique Identifier : AIDSLINE ICA7/1212091; Comi G; Medaglini S; Nemni R; Quattrini A; Comola M; Galardi G; Canal N; Castagna A; Lazzarin A; Dept. of Neurology, University of Milan, Scient. Inst. S.; Raffaele; Peripheral nerve damage in HIV infections can be due to various pathogenetic mechanisms. As a consequence, different types of peripheral neuropathy (PN), have been described. We evaluate different neurophysiological and morphological aspects in AIDS and HIV+ patients.
Neurological involvement in HIV patients: A negative prognostic factor for developing AIDS.: Int Conf AIDS. 1991 Jun 16-21;7(1):212 (abstract no. M.B.2121). Unique Identifier : AIDSLINE ICA7/1212191; Medaglini S; Comi G; Galardi G; Locatelli T; Comola M; Nemni R; Quattrini A; Lazzarin A; Castagna A; Canal N; IV Neurologic Department Scient. Inst. H San Raffaele, University; of Milan; The literature is lacking of data concerning the prognostic significance of the presence of neurological complications to assess the risk to develop AIDS. We studied 40 pts consecutively admitted to the Department of Infectious Diseases, University of Milan - H Sacco -. Neurological evaluation included clinical examin
Subjective vs objective - A study of patients' perceived state and its relationship to their psychometry.: Int Conf AIDS. 1991 Jun 16-21;7(1):212 (abstract no. M.B.2122). Unique Identifier : AIDSLINE ICA7/1212291; Winwood MA; Hopper C; Walls F; Wycherley H; Kocsis AE; Neuropsychology of AIDS Research, St. Mary's Hospital, London,; U.K.; OBJECTIVES: To assess the accuracy of perceived neuropsychological impairment and change over time in HIV+ hospital inpatients. To compare these perceptions with their performance on an objective neuropsychological test battery. METHODS: In patients are approached during the first few days of their hospital admission
Structure and validation of a clinical database for the study of neurological impairment in HIV+ patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):212 (abstract no. M.B.2123). Unique Identifier : AIDSLINE ICA7/1212391; Ruiz L; Sandrini G; Turla M; Minoli L; Grossi P; Nappi G; NeuroAIDS Unit, IRCCS, University of Pavia, Italy; OBJECTIVE: The aim of the AIDS Research Project was to develope an Information System for a correct management of epidemiological, clinical and laboratory data of a large number of HIV+ patients. Within this project the working out of a clinical database for the study of the Nervous System involvement in HIV+ patients
Chronic uveitis and progressive encephalytis associated with long term HIV-2 infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):213 (abstract no. M.B.2124). Unique Identifier : AIDSLINE ICA7/1212491; Terrinna AM; Nunes JFM; Teixeira C; Gameiro P; Veloso M; Lima C; Leitao O; Centro de Virologia, Inst. Portugues de Oncologia, Lisboa,; Portugal; Two cases of CNS disease etiologically associated with HIV-2 infection has been reported, most cases of CNS impairment corresponding to opportunistic infections. We report the case of a 15 years old Caucasian male, presenting a chronic uveitis of non determined cause for the last 3 years. In the last 3,5 months progre
HTLV-I in blood donors and neurology patients observations from Dakar, Senegal: Int Conf AIDS. 1991 Jun 16-21;7(1):213 (abstract no. M.B.2125). Unique Identifier : AIDSLINE ICA7/1212591; Thiam D; Ndiaye IP; Diop AG; Kabou F; Lequin RM; Diakhate L; Centre National de Transfusion Sanguine, Dakar, Senegal; OBJECTIVE: To obtain insight in the epidemiology of HTLV-I infection in blooddonors and patients from a department of neurology at the urban area of Dakar, Senegal . METHODS: 434 blood donations were screened and blood samples from 121 patients with various neurological disorders,
The relationship between HIV symptomatology and affect.: Int Conf AIDS. 1991 Jun 16-21;7(1):213 (abstract no. M.B.2126). Unique Identifier : AIDSLINE ICA7/1212691; Dunbar N; Perdices M; Grunseit A; Cooper D; National Centre in HIV Epidemiology and Clinical Research,; University of New South Wales, Sydney, Australia; AIM: To determine if HIV symptoms predict objective measures of anxiety and depression. METHOD: Self-report symptoms and immunological measures were collected for 207 homo/bisexual men (AIDS=34, ARC=72, HIV+=101). The affect measures used were the State/Trait Anxiety Questionnaire and the CES-D Depression Rating Scale
Zidovudine treatment and incidence of progressive multifocal leukoencephalopathy (PML) in AIDS patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):213 (abstract no. M.B.2127). Unique Identifier : AIDSLINE ICA7/1212791; Galli M; Rizzardini G; Vago L; Castagna A; Isabella L; Coen M; Fasan M; Albarello V; Costanzi GC; Moroni M; Infectious Diseases Dep. and V Chair of Pathology, L. Sacco; Hospital, Milan, Italy; OBJECTIVE: Recent observations suggest possible response of PML to AZT . The aim of this study is to evaluate retrospectively the influence of AZT on the incidence of PML in a autoptical case file. METHODS: Reviewing an autoptical series of 485 AIDS ca
A retrospective study of clinical and cerebrospinal fluid parameters of 11 cases of meningoencephalitis due to mycobacterium avium-intracellulare in AIDS patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):214 (abstract no. M.B.2128). Unique Identifier : AIDSLINE ICA7/1212891; Hadad DJ; Cavalcante NJ; Araujo A; Petry TC; Palaci M; Ferrazoli L; Martins MC; Centro de Referencia e Treinamento - AIDS; The presence of Mycobacterium avium-intracellulare is being increasingly described from a few sites in AIDS patients however its isolation from central nervous system (C.N.S) is very rare particularly in Brazil . This report document the presence of Mycobacterium avium-intracellular
Neuropsychological consequences of HIV infection in drug addicts.: Int Conf AIDS. 1991 Jun 16-21;7(1):214 (abstract no. M.B.2129). Unique Identifier : AIDSLINE ICA7/1212991; Cominelli C; Cappa SF; Signorini L; Vangi D; Vignolo LA; Chiodera A; Carosi G; Neurological Clinic, University of Brescia, Italy; OBJECTIVE: investigation of neuropsychological performance in HIV negative, asymptomatic HIV positive and AIDS-affected drug addicts in comparison to normal controls to evaluate the consequences of drug abuse and HIV infection on cognitive function. METHODS: 70 subjects (20 controls, 15 HIV-, 20 HIV+ and 15 AIDS drug
Gerstman syndrome and HIV encephalitis.: Int Conf AIDS. 1991 Jun 16-21;7(1):214 (abstract no. M.B.2130). Unique Identifier : AIDSLINE ICA7/1213091; Cirelli A; Ciardi M; Salotti A; Rossi F; University of Rome La Sapienza, Rome, Italy; OBJECTIVE: At the Institute of Infectious Diseases we have studied clinical forms of HIV encephalitis to establish their features and their evolution also in relation to zidovudine therapy. We describe the unusual Gerstman syndrome (agraphia, acalculia, fingeragnosia) in an AIDS patient. METHODS: We have studied 25 co
The correlation between AIDS dementia complex and the presence of macrophage tropic viruses in the cerebrospinal fluid of patients infected with HIV-1.: Int Conf AIDS. 1991 Jun 16-21;7(1):214 (abstract no. M.B.2131). Unique Identifier : AIDSLINE ICA7/1213191; Evans L; Hurran L; Mooney J; Penny R; Cooper DA; Brew BJ; Centre for Immunology, St. Vincent's Hospital, Sydney, Australia; OBJECTIVES: Neurotropic strains of HIV-1 have been shown to replicate more efficiently in macrophages than in T cells. The aim of this study was to assess the prevalence and nature of such strains in patients with/without AIDS dementia complex (ADC). METHODS: Cerebrospinal fluid (C
Otoneuroogical abnormalities in AIDS patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):215 (abstract no. M.B.2132). Unique Identifier : AIDSLINE ICA7/1213291; Salami A; Cordone G; Melagrana A; Barbieri M; Morandi N; Rizzo F; University of Genoa, O.R.L. Clinic, S. Martino Hospital, Genoa,; Italy; In order to study subclinical Cortical Nervous System (CNS) disturbances in healthy HIV-infected patients and in AIDS patients without neurological symptoms, we used two sensitive neurophysiological tests. Auditory Brainstem Response audiometry (ABR) and oculometer tests: Vestibular Ocular Reflex (VOR), Optokinetik My
Headache in HIV-infected patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):215 (abstract no. M.B.2133). Unique Identifier : AIDSLINE ICA7/1213391; Trenkwalder C; Schielke E; Pfister W; Einhapl KM; Department of Neurology, Klinikum Grosshadern, Munich, Germany; Headache represents a frequent symptom in HIV-infected patients and can be due to different etiologies. We investigated prospectively 51 out of 300 HIV-infected patients who developed headache of recent onset with a standard interview, EEG, CCT or MRI and lumbar puncture when indicated to classify types of headache, t
Mechanism of toxicity of AZT and other anti-HIV-1 dideoxynucleosides.: Int Conf AIDS. 1991 Jun 16-21;7(1):215 (abstract no. M.B.2134). Unique Identifier : AIDSLINE ICA7/1213491; Hobbs G; Keilbaugh SA; Prusoff WA; Simpson MV; Dept. of Biochemistry and Cell Biology, State University of New; York, Stony Brook, NY USA; OBJECTIVES: We aim to elucidate the role of mtDNA in AZT-induced anemia and ddC- (and ddI-) induced peripheral neuropathy (PN), and to use this information to develop less toxic dideoxynucleoside (ddN) analogs. RATIONALE: DNA polymerase gamma is known to incorporate d
Psychiatric disorder in HIV disease: description of 200 referrals to a liaison psychiatry service.: Int Conf AIDS. 1991 Jun 16-21;7(1):215 (abstract no. M.B.2135). Unique Identifier : AIDSLINE ICA7/1213591; Douzenis A; Brener N; Catalan J; Meadows J; Academic Department of Psychiatry, Westminster Hospital. London.; SW1. United Kingdom; AIMS: To describe the problems and characteristics of HIV patients referred to a psychiatric service. METHOD: Descriptive study of 200 consecutive referrals with systematic collection of data about the patients, their diagnosis and treatment. RESULTS: The majority were males (98%), mean age 36 (SD=7.9), the majority s
HIV and psychiatric illness - How healthy are the normal controls? A prospective study of a large volunteer group.: Int Conf AIDS. 1991 Jun 16-21;7(1):216 (abstract no. M.B.2136). Unique Identifier : AIDSLINE ICA7/1213691; Gaist PA; Jacobsen F; Alcohol, Drug Abuse, and Mental Health Administration, National; Institute of Mental Health, Bethesda, Maryland, USA; OBJECTIVE: Although a control group is frequently considered critical in psychiatric research, few studies have investigated the characteristics of persons volunteering to be controls. METHODS: A prospective study using a newspaper recruitment screening questionnaire for healthy normal volunteers (without psychiatric,
Impact of HIV infection on neurodevelopment in perinatally infected children.: Int Conf AIDS. 1991 Jun 16-21;7(1):216 (abstract no. M.B.2137). Unique Identifier : AIDSLINE ICA7/1213791; Durako S; Muenz L; Hittelman J; Nozyce M; Willoughby A; Westat, Inc., Rockville, MD, USA; OBJECTIVE: To assess the impact of HIV infection on neurodevelopment by comparing repeated measurements of infected children (I), uninfected children of HIV+ women (U), and controls (C). METHODS: Bayley Scales of Infant Development (MDI/PDI) were administered at 3, 6, 9, 12, 15, 18, and 24 months to children enrolled
Correlation between urinary and liquoral serotonin and cathecholamine levels and psychopathology during HIV infection.: Int Conf AIDS. 1991 Jun 16-21;7(1):216 (abstract no. M.B.2138). Unique Identifier : AIDSLINE ICA7/1213891; Marazziti D; Perretta P; Nisita C; Galli L; Cassano GB; Scasso A; Gori S; Della Santa M; Istituto di Clinica Psichiatricca, Universita di Pisa; OBJECTIVE: The aim of this study was to investigate if there are deficits of the main biogenic amine metabolites in urine and liquor of HIV infected, as well as to investigate the eventual correlations between these metabolites and psychiatric disorders, especially with mood disorders. SUBJECTS AND METHODS: We include
HIV related cognitive impairment: associated management and residential problems.: Int Conf AIDS. 1991 Jun 16-21;7(1):216 (abstract no. M.B.2139). Unique Identifier : AIDSLINE ICA7/1213991; Boccellari A; Dilley JW; Barlow I; Hernandez S; Haskell W; Steketee M; San Francisco General Hospital, San Francisco, California, USA; OBJECTIVE: To estimate the number of HIV spectrum patients with significant residential placement or management problems (Res Pro) secondary to HIV-related cognitive impairment (HCI) and to describe salient characteristics of HCI associated with management and Res Pro. METHODS: To assess presence and severity of HCI (
P.Carinii induces-TNF-alpha production by macrophages.: Int Conf AIDS. 1991 Jun 16-21;7(1):217 (abstract no. M.B.2140). Unique Identifier : AIDSLINE ICA7/1214091; Tamburrini E; De Luca A; Ventura G; Maiuro G; Siracusano A; Ortona E; Antinori A; Clinica Malattie Infettive, Universita Cattolica S. Cuore, Roma,; Italy; OBJECTIVE: To test the ability of P.carinii to induce release of cytokines by monocytes/macrophages from healthy adult donors. METHODS: 2.10(5)/ml monocytes or monocyte derived macrophages (mdM) (greater than 7 days cultures) were exposed to P.carinii cysts (from Sprague-Dawley rats) at the concentration of 10(3) and
Detection of HTLV-1 or HTLV-2 in HIV infected patients with lymphoid interstitial pneumonitis.: Int Conf AIDS. 1991 Jun 16-21;7(1):217 (abstract no. M.B.2141). Unique Identifier : AIDSLINE ICA7/1214191; Couderc LJ; Matheron S; Clauvel JP; Caubarrere I; Desgranges C; Hopital Foch, Suresnes, France; OBJECTIVE: To examine the presence of human T lymphocytic virus type 1 (HTLV-1) or type 2 (HTLV-2) in HIV infected patients with lymphoid interstitial pneumonitis. PATIENTS AND METHODS: Patients: 3 male patients (1 caucasian homosexual man; 2 heterosexual black patients). None was IV drug user. The two black patients
The diagnostic value of simple investigations for PCP.: Int Conf AIDS. 1991 Jun 16-21;7(1):217 (abstract no. M.B.2142). Unique Identifier : AIDSLINE ICA7/1214291; Smith D; Boag F; Barton S; Davies S; Gazzard B; Westminster Hospital, London, England; OBJECTIVE: To produce a clinical algorithm of appropriate simple investigations for predicting Pneumocystis carinii pneumonia (PCP) in high risk patients. METHODS: All HIV infected patients presenting to this unit with suspected PCP were assessed. Features considered to be suggestive of PCP were: 1) symptoms of dyspno
The impact of the indirect fluorescent monoclonal antibody (IFA) stain on the detection of Pneumocystis carinii (PC) compared with conventional stains.: Int Conf AIDS. 1991 Jun 16-21;7(1):217 (abstract no. M.B.2143). Unique Identifier : AIDSLINE ICA7/1214391; Jenny EB; Widrow CA; Coburn KD; Choi Y; McCabe KM; Graham FG; Wittner M; Harris CA; Bronx Municipal Hospital Center/Albert Einstein College of; Medicine, Bronx, New York, USA; OBJECTIVE: To investigate the incremental contribution made by the addition of the IFA stain to a battery of conventional stains including Gram-Weigert (GW) and methenamine-silver (MS) used for the detection of PC in respiratory secretions. METHODS: Laboratory records for the period 7/1/90 to 1/15/91 were reviewed. Th
Prognostic value of the evaluation of BAL populations in patients with HIV-1 infection and pulmonary involvement.: Int Conf AIDS. 1991 Jun 16-21;7(1):218 (abstract no. M.B.2144). Unique Identifier : AIDSLINE ICA7/1214491; Siviero F; Trentin L; Zambello R; Gritti F; Poletti V; Cipriani A; Carretta M; Cadrobbi P; Agostini C; Semenzato G; Cittadella Hospital, 35128 PADUA, Italy; OBJECTIVE: To investigate the prognostic utility of the morphological and immunologic evaluation of bronchoalveolar lavage (BAL) cell populations in determining the death hazard in patients with HIV-1-related pulmonary complications. METHODS: BAL cell subpopulations obtained from 73 patients with first confirmed episo
Role of high resolution computed tomography (HRCT) in the valuation of AIDS suspected infectious pulmonary diseases.: Int Conf AIDS. 1991 Jun 16-21;7(1):218 (abstract no. M.B.2145). Unique Identifier : AIDSLINE ICA7/1214591; Morresi A; Santoro D; Rinaldi E; Ferzi A; Scarpellini P; Bertelli D; Department of Radiology, S. Anna Hospital, Como - Italia; OBJECTIVE: The role of HRCT of the lungs in patients with AIDS is discussed. METHODS: Eighteen patients with AIDS and clinically suspected infectious pulmonary abnormalities underwent chest X-rays and HRCT. Radiological findings were compared with bronchoalveolar lavage (BAL) in 14 out of 18 patients and with clinical
Steroid-related bronchopulmonary kaposi sarcoma in a homosexual man with AIDS.: Int Conf AIDS. 1991 Jun 16-21;7(1):218 (abstract no. M.B.2146). Unique Identifier : AIDSLINE ICA7/1214691; Medrano FJ; Aguado I; Andreu J; Calderon E; Sanchez-Quijano A; Lissen E; Leal M; Virgen del Rocio University Hospital, Seville, Spain; INTRODUCTION: Kaposi s sarcoma (KS) is the most frequently observed tumor in patients with AIDS, particularly in male homosexual. Recently, a few cases of patients infected by HIV-1 in whom the administration of steroids induced or activated a cutaneous KS which regressed after discontinuation of treatment, have repor
Significance of cytomegalovirus (CMV) detection in bronchoalveolar lavage (BAL) in AIDS patients (PS) with interstitial pneumonia. A preliminary study.: Int Conf AIDS. 1991 Jun 16-21;7(1):218 (abstract no. M.B.2147). Unique Identifier : AIDSLINE ICA7/1214791; Costa JR; Rubio R; Cervero M; Crespo L; Carnevali D; Guijarro C; Casillas A; Pulido F; Delgado R; Villena V; Hospital 12 de Octubre. Universidad Complutense. Madrid, Spain; OBJECTIVE: To assess the potential usefulness of CMV culture from BAL for the diagnosis of CMV pneumonia in ps with AIDS. METHODS: 24 episodes of interstitial pneumonia in 22 AIDS ps during a 18 months period were examined. We correlated the detection of CMV in
Strongyloides stercoralis hyperinfection; diagnosis by transbronchial lung biopsy.: Int Conf AIDS. 1991 Jun 16-21;7(1):219 (abstract no. M.B.2148). Unique Identifier : AIDSLINE ICA7/1214891; Leite KR; Fernandez A; Miziara AC; Hospital Sirio Libanes, Sao Paulo, Brazil; Strongyloides stercoralis (SS) is an endemic parasitosis in tropical and subtropical areas of the world and immunodepressed patients may have severe infections with this parasite. The authors present 3 patients with acquired immuno deficiency syndrome with interstitial pneumonitis demonstrated on chest X ray. They wer
Comparison of 99Tc-human policlonal immunoglobulin (99Tc-HIG) scintigraphy and 67gallium-citrate (67Ga).: Int Conf AIDS. 1991 Jun 16-21;7(1):219 (abstract no. M.B.2149). Unique Identifier : AIDSLINE ICA7/1214991; Galli G; Salvatori M; Antoni M; Pirronti T; Ventura G; Maiuro G; Ortona L; Istituto di Medicina Nucleare, Roma; OBJECTIVE: To assess the efficacy and safety of 99Tc-HIG scintigraphy versus 67Ga scan in AIDS patients with pulmonary infections. METHODS: Eleven AIDS patients (pts) (9 males 2 females; median age 31 y.o. range 25-44) with pulmonary infections (5 Pneumocystis carinii (PCP), 2 tuberculosis (TBC), 1 Mycobacterium avium
Alveolar hemorrhage among HIV individuals.: Int Conf AIDS. 1991 Jun 16-21;7(1):219 (abstract no. M.B.2150). Unique Identifier : AIDSLINE ICA7/1215091; Quieffin J; Brechot JM; Poubeau P; Laaban JP; Capron F; Rochemaure J; Department of Respiratory Medicine, Hotel Dieu Hospital, Place du; Parvis Notre Dame - Paris - France; OBJECTIVE: To describe the frequency, the causes and the clinical presentation of alveolar hemorrhage (AH) diagnosed by broncho-alveolar lavage (BAL) in HIV individuals. METHODS: All BAL performed in HIV individuals between September 1987 and December 1990 showing evidence of AH (hemosiderin laden macrophage score gre
Use of high resolution CT scan (HR CT scan) in the management of seropositive patients with pulmonary complications.: Int Conf AIDS. 1991 Jun 16-21;7(1):219 (abstract no. M.B.2151). Unique Identifier : AIDSLINE ICA7/1215191; Leclercq P; Lambrinidis M; Manquat G; Coulomb M; Micoud M; Maladies Infectieuses, Grenoble - France; AIMS of the study: This study was conducted to evaluate prospectively the use of HR CT scan in the screening of pulmonary complications in HIV disease and to focus on the early diagnosis of pneumocystis carinii pneumonia (PCP) by the HR CT scan technique. METHODS: All HIV positive patients who were admitted in our cen
The humoral response to specific antigen from M. tuberculosis in AIDS patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):220 (abstract no. M.B.2152). Unique Identifier : AIDSLINE ICA7/1215291; Saad MH; Ferreira MA; Kritski AL; Werneck EB; Cordeiro SG; Horta RM; Cavalcante SC; Santos VG; Fonseca LS; SH-FIOCRUZ, Rio de Janeiro, Brazil; To test whether antibodies (IgG) to specie specific glycolipid (SL-IV) from M. tuberculosis are formed in HIV infection, we investigated by ELISA sera from 86 patients with pulmonary tuberculosis (18 had AIDS by CDC criteria; 24 were HIV+ without AIDS and 44 were HIV-) and 104 non-tuberculous controls (19 had AIDS; 40
A comparison of five bronchodilator agents and two delivery systems for the prophylaxis of nebulised pentamidine induced bronchoconstriction.: Int Conf AIDS. 1991 Jun 16-21;7(1):220 (abstract no. M.B.2153). Unique Identifier : AIDSLINE ICA7/1215391; Leigh T; Wiggins J; Gazzard BG; Collins JV; Department of Respiratory Medicine, Westminster Hospital, London,; UK; OBJECTIVE: We set out to characterise the severity and duration of bronchial narrowing (BN) induced by nebulised pentamidine isethionate (PI), and to compare the prophylactic efficacy of 2 delivery systems and 5 bronchodilator agents in preventing PI induced BN. METHODS: Sixty three HIV positive patients without chron
TB and HIV: a review of all cases in British Columbia during 1984-1990.: Int Conf AIDS. 1991 Jun 16-21;7(1):220 (abstract no. M.B.2154). Unique Identifier : AIDSLINE ICA7/1215491; Korzeniewska-Kosela M; Fitzgerald JM; Vedal S; Allen EA; Schechter MT; Lawson L; Phillips P; Montaner JS; St. Paul's Hospital AIDS Research Group, Vancouver, Canada; OBJECTIVE: To review all cases of TB/HIV in the province of British Columbia, Canada diagnosed from 1984-90. METHODS: Cases were identified from the provincial TB registry and the St Paul s Hospital-HIV Clinic. All cases were reviewed with regard to clinical features, treatment and
Breakthrough-pneumocystis-carinii-pneumonia (PCP) in HIV-patients receiving primary (PP) or secondary prophylaxis (SP) with aerosolized pentamidine (AP).: Int Conf AIDS. 1991 Jun 16-21;7(1):220 (abstract no. M.B.2155). Unique Identifier : AIDSLINE ICA7/1215591; Ewig S; Rockstroh J; Niese D; Medizinische Klinik der Universitat Bonn; OBJECTIVE: To study incidence, clinical presentation and therapeutic results of PCP and PCP-relapses (PCP-R) under AP. METHODS: Since May 1989, 50 HIV-infected pts were started on AP. 30 pts received PP. 20 pts SP. PP was begun in pts with a CD4+ count of less than 100/mul. 60 mg of AP were administered biweekly by a
Pentamidine is chemotactic for human neutrophils and monocytes.: Int Conf AIDS. 1991 Jun 16-21;7(1):221 (abstract no. M.B.2156). Unique Identifier : AIDSLINE ICA7/1215691; Tepass T; Wassermann K; Schell-Frederick E; Internal Medicine I, University of Cologne, FRG; OBJECTIVE: To investigate the mechanisms underlying the prophylactic action of inhaled Pentamidine against Pneumocystis carinii pneumonia (PCP). PATIENTS AND METHODS: Peripheral blood mononuclear cells (PBMC) and neutrophils were isolated from normal donors and 10 HIV-infected patients (WR3-5) by density gradient cent
Induced sputum (IS) for detection of mycobacteria in immunodeficient HIV positive patients (IHPP).: Int Conf AIDS. 1991 Jun 16-21;7(1):221 (abstract no. M.B.2157). Unique Identifier : AIDSLINE ICA7/1215791; Tocci G; Antonelli L; Bordi E; D'amato C; Falco MC; Noto P; Visco G; U.S.L. RM/10 L.Spallanzani Hospital for Infectious Diseases.; Rome, Italy.; OBJECTIVE: To assess the efficacy of IS for detection of mycobacteria in IHPP. METHODS: IS was examined in 191 consecutive patients with suspected respiratory disease. Sputum was induced by inalation of 20 ml of 3% saline through an ultrasonic nebuliser. In 108 patients (56,5%), who had spontaneous sputum (SS), both S
Pleural effusion due to opportunistic infection in AIDS.: Int Conf AIDS. 1991 Jun 16-21;7(1):221 (abstract no. M.B.2158). Unique Identifier : AIDSLINE ICA7/1215891; Denis M; Chouaid C; Cadranel J; Gonzales G; Mayaud C; Rothschild, St Antoine Hospital, Paris, France; The objective of this retrospective study is to analyse the frequency, the pathogens responsible and the clinical data of pleural effusion (P.E) due to opportunistic infection (O.P.E) in AIDS pts. Incidence and responsible pathogens: between 1988 and 1990, 75 HIV-infected pts were admitted to the East Paris Hospitals
Predictors of pulmonary tuberculosis in sputum-negative Brazilian AIDS patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):221 (abstract no. M.B.2159). Unique Identifier : AIDSLINE ICA7/1215991; Schechter M; Teixeira ML; Carvalho AC; Perez M; AIDS Program, Federal University of Rio de Janeiro, Brazil; OBJECTIVE: To derive a rule based on simple clinical and lab data for predicting a diagnosis of pulmonary tuberculosis (Tb) in AIDS patients whose sputum investigation is negative. METHODS: Chart review of AIDS patients submitted to diagnostic bronchoscopy between Dec 88 - Feb 90 in a hospital in
A comparison of the effectiveness of three drug regimens in the prevention of Pneumocystis carinii pneumonia in AIDS and ARC patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):222 (abstract no. M.B.2160). Unique Identifier : AIDSLINE ICA7/1216091; Martin M; Cox P; Styer C; Beall G; Harbor-UCLA Medical Center, Torrance, CA, USA; OBJECTIVE: To determine and compare the incidence of Pneumocystis carinii pneumonia (PCP) in AIDS and ARC patients on three PCP prophylaxis drug regimens. METHODS: A retrospective chart review of 211 patients, with CD-4 cell counts less than 200/mm(3) or a diagnosis of AIDS who received either trimethoprim/sulfamethox
Does a precipitous decline in the diffusing capacity in asymptomatic HIV-infected persons predict the presence of PCP or other pulmonary infection?: Int Conf AIDS. 1991 Jun 16-21;7(1):222 (abstract no. M.B.2161). Unique Identifier : AIDSLINE ICA7/1216191; Kyale P; Rosen M; Game S; Markowitz N; Johnson C; Saravolatz L; Fulkerson W; Henry Ford Hospital Detroit, MI, USA; OBJECTIVE: To determine whether a precipitous decline greater than or equal to 20%) in diffusing capacity (Decline-DLCO) without new pulmonary symptoms or new chest x-ray abnormalities (CXR-Abn) predicts the presence of PCP or other pulmonary infections in HIV-infected patients (pts.). METHODS: In a multicenter study
Antibody responses to a Pneumocystis carinii antigen in HIV infected patients with and without P. carinii-pneumonia.: Int Conf AIDS. 1991 Jun 16-21;7(1):222 (abstract no. M.B.2162). Unique Identifier : AIDSLINE ICA7/1216291; Lundgren B; Lundgren JD; Jensen K; Mathiesen L; Nielsen JO; Kovacs JA; University of Copenhagen, Hvidovre Hospital, Denmark; OBJECTIVE: To investigate changes in antibody responses to one of the major surface antigens of P. carinii (gp95) in HIV positive patients who underwent bronchoscopy for pulmonary symptoms. METHOD: Antibody responses to the purified human P. carinii surface antigen (gp 95) were determined by IgG ELISA, as a ratio of t
Role of the Il-2 receptor in the mechanisms accounting for T cell alveolitis in the lung of HIV-1 patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):222 (abstract no. M.B.2163). Unique Identifier : AIDSLINE ICA7/1216391; Zambello R; Trentin L; Crivellaro C; Salmaso L; Milani A; Cipriani A; Agostini C; Semenzato G; Padua University, PADUA, Italy; OBJECTIVE: To investigate the mechanisms accounting for the proliferation of CD8+ cells in the lung of HIV-1+ patients we analyzed the role of two lymphokines which are involved in the mechanisms of T cell proliferation, i.e., interleukin-2 ( IL-2 ) and interleukin-4 (IL
The clinical utility of screening pulmonary function tests (PFTs) in patients eligible for PCP prophylaxis.: Int Conf AIDS. 1991 Jun 16-21;7(1):223 (abstract no. M.B.2164). Unique Identifier : AIDSLINE ICA7/1216491; Doyle R; Chernoff D; Golden J; Department of Medicine, University of California, San Francisco,; California, USA; OBJECTIVE: To determine the clinical utility of PFTs in screening for PCP in HIV+ patients eligible for aerosolized pentamidine prophylaxis. METHODS: We studied all patients referred to initiation of aerosolized pentamidine prophylaxis over an 8 month period. Patients with symptoms suggestive of active pulmonary disea
Pleural effusion due to Kaposi's sarcoma: diagnostic value of clinical examination, chest x-ray and thoracocentesis.: Int Conf AIDS. 1991 Jun 16-21;7(1):223 (abstract no. M.B.2165). Unique Identifier : AIDSLINE ICA7/1216591; Chouaid C; Cadranel J; Winter C; Mehoyas MC; St Antoine Hospital, Paris, FRANCE; In HIV-infected patients (pts) with pleural effusion (P.E), the usual diagnostic problem is to distinguish between the two main causes which are infection and Kaposi s sarcoma (K.S). The objective of this retrospective study is to determine the contribution of clinical and radiological examination and analysis of pleu
Mycobacterium tuberculosis (TB) in HIV+/HIV-homo/bisexual men (HB), intravenous drug users (IVDU) and women with heterosexually-acquired HIV (HF).: Int Conf AIDS. 1991 Jun 16-21;7(1):223 (abstract no. M.B.2166). Unique Identifier : AIDSLINE ICA7/1216691; Markowitz N; Reichman L; Kvale P; Saravolatz L; Johnson C; Hultsing J; Game S; Henry Ford Hospital, Detroit, MI, USA; OBJECTIVE: The study examines epidemiologic features of TB infection in HIV+ and HIV- patients (pts). METHODS: PACS is a prospective multicenter study aimed at early detection of pulmonary processes related to HIV disease. Two groups of HIV+ pts were followed: A - 505 asymptomatics with CD4/mm(3) (CD4) greater than or
Pulmonary toxoplasmosis in AIDS patients: incidence in northeastern Italy.: Int Conf AIDS. 1991 Jun 16-21;7(1):223 (abstract no. M.B.2167). Unique Identifier : AIDSLINE ICA7/1216791; Libanore M; Sighinolfi L; Trevisani L; Poletti V; Gritti F; Divisione Malattie Infettive e Ia Divisione Medica, Arcispedale; S. Anna, Ferrara, Italy; OBJECTIVE: To evaluate the incidence of pulmonary toxoplasmosis in AIDS patients of the Northeastern Italy . METHODS: From January 1984 to December 1990, 352 AIDS patients were admitted to Department of Infectious Diseases of Ferrara and Bologna, Italy. 286 (81,2%) with clinical and
Alveolar macrophages from patients with AIDS and ARC constitutively express mRNA for TNF alpha and release TNF alpha.: Int Conf AIDS. 1991 Jun 16-21;7(1):224 (abstract no. M.B.2168). Unique Identifier : AIDSLINE ICA7/1216891; Agostini C; Zambello R; Trentin L; Garbisa S; Francia Di Celle P; Ralse E; Poletti V; Foa R; Semenzato G; Padua University, PADUA, Italy; OBJECTIVE: To study the capacity of alveolar macrophages (AMs) from HIV-1 infected patients to release tumor necrosis factor alpha (TNF alpha). METHODS: Resting AMs freshly recovered from the bronchoalveolar lavage fluid of 8 patients with AIDS and 7 with ARC were tested for their ability to destroy TNF alpha-suscepti
Long term survival following intensive care support in HIV patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):224 (abstract no. M.B.2169). Unique Identifier : AIDSLINE ICA7/1216991; Casalino E; Wolff M; Gaudebout C; Bedos JP; Lucet JC; Fegueux S; Vachon F; Hopital Bichat-Claude Bernard, Paris, France; OBJECTIVE: To determine the long term survival for the HIV patients (pts.) who survived the Intensive Care Unit (ICU) stay. METHODS: From Jan. 89 to Dec. 89, 124 HIV patients were admitted to our ICU. The causes of admission were: PCP revealing AIDS (PCP r) = 22 pts.; PCP non revealing AIDS (PCP nr) = 15 pts.; neurolo
Cutaneous hypersensitivity reactions (CHR) to thiacetazone among HIV-1 seropositive tuberculosis (TB) patients, Nairobi.: Int Conf AIDS. 1991 Jun 16-21;7(1):224 (abstract no. M.B.2170). Unique Identifier : AIDSLINE ICA7/1217091; Nunn P; Wasunna K; Kwanyah G; Gathua S; Imalingat A; Lucas S; Gilks C; Brindle R; Omwega M; Were J; et al; Kenya Medical Research Institute, Nairobi, Kenya; OBJECTIVES: To compare relative incidence of CHR among HIV+ and HIV- TB patients. METHODS: 287 consecutive patients with newly diagnosed TB presenting to IDH and Ngaira Avenue Chest Clinic were enrolled as part of a longitudinal follow-up study. 227 patients were treated with 1 month of streptomycin plus 12 months thi
Multiple opportunistic infections of the lung in acquired immunodeficiency syndrome (AIDS). A comparison of 42 post mortem findings with premortem diagnosis.: Int Conf AIDS. 1991 Jun 16-21;7(1):224 (abstract no. M.B.2171). Unique Identifier : AIDSLINE ICA7/1217191; Hofman P; Mondain V; Toussaint MG; Laffont C; Galibert A; Cassuto JP; Dellamonica P; Department of Pathology, University of Nice - FRANCE; OBJECTIVE: We studied 42 post mortem cases of lung s multiple opportunistic infection in patients (pts) with AIDS. We compared these lesions with premortem diagnosis performed on transbronchial biopsy (TBB), bronchoalveolar lavage (BAL) and open lung biopsy (OLB). METHODS: 101 consecutive autopsies were performed betw
A nosocomial outbreak of isoniazid- and streptomycin-resistant tuberculosis (TB) among AIDS patients at a new york city hospital.: Int Conf AIDS. 1991 Jun 16-21;7(1):225 (abstract no. M.B.2172). Unique Identifier : AIDSLINE ICA7/1217291; Edlin BR; Tokars JI; Grieco MH; Crawford J; Williams J; Sordillo E; Ong K; Walter A; Jarvis WR; Holmberg SD; CDC, Atlanta, GA, USA; OBJECTIVE: In 1990, the first recognized clusters of primary multiply resistant TB (MRTB) among hospitalized U.S. AIDS patients were reported. We investigated a cluster of 18 isoniazid- and streptomycin-resistant TB (ISRTB) cases among homosexual AIDS patients occurring over 16 months in a New York City hospital, to a
Home-based PCP prophylaxis using aerosolized pentamidine.: Int Conf AIDS. 1991 Jun 16-21;7(1):225 (abstract no. M.B.2173). Unique Identifier : AIDSLINE ICA7/1217391; Duguay D; Falutz J; Tsoukas CM; McGill AIDS Centre, Montreal, Canada; OBJECTIVE: PCP prophylaxis using aerosolized pentamidine (AP) reduces PCP incidence in susceptible HIV-infected patients (pts). Given the large numbers of pts at risk for PCP, effective home-based treatment would be practical. We analyzed the outcome of pts receiving home AP. METHODS: Pts received 60 mg AP at home eve
A rise in drug-resistant mycobacterium tuberculosis (Mtb) in patients with HIV infection, substance abuse, and homelessness.: Int Conf AIDS. 1991 Jun 16-21;7(1):225 (abstract no. M.B.2174). Unique Identifier : AIDSLINE ICA7/1217491; DePalo VA; Salomon N; Kolokathis A; Perlman DC; Pumerantz A; Raucher B; Beth Israel Medical Center, The Mount Sinai School of Medicine,; New York, NY, USA; OBJECTIVE: To describe the characteristics of patients with drug-resistant Mtb. METHODS: We retrospectively reviewed mycobacteriology logbooks and patient medical records from 1986 to 1990 for cases of Mtb. Isolates were tested for sensitivity to 0.2 and 1.0 ug/ml isoniazid (INH), 1.0 and 2.0 ug/ml rifampin (RIF), 2.0
Reduced expression of phenotypic markers on alveolar macrophages in HIV infected patients with pneumonitis.: Int Conf AIDS. 1991 Jun 16-21;7(1):225 (abstract no. M.B.2175). Unique Identifier : AIDSLINE ICA7/1217591; Bray DH; Squire SB; Bagdades EK; Mulvenna PM; Johnson MA; Poulter LW; Departments of Immunology, Virology and Thoracic Medicine, Royal; Free Hospital and School of Medicine, Hampstead, London NW3 2QG,; UK; OBJECTIVE: To investigate whether occurrence of pneumonitis in HIV infected patients is related to phenotypic changes in alveolar macrophages (AM). METHODS: AM were obtained by bronchoalveolar lavage (BAL) from 35 HIV infected patients presenting with pneumonitis. AM from 15 transplant recipients with pneumonitis and
Prolonged chemotherapy for tuberculosis following standard short course treatment among HIV-infected persons in Zaire.: Int Conf AIDS. 1991 Jun 16-21;7(1):226 (abstract no. M.B.2176). Unique Identifier : AIDSLINE ICA7/1217691; Mukadi Y; Perriens J; Kaboto M; Roscigno G; Prignot J; Portaels F; Ngamboli K; Williame J; Piot P; St Louis M; Projet SIDA, Kinshasa, Zaire; OBJECTIVES: Evaluate the need for and efficacy of prolonged treatment (Rx) for primary pulmonary tuberculosis (TB) in HIV-infected persons. METHODS and RESULTS: 200 HIV- and 315 HIV+ patients with pulmonary TB were Rx d with rifampicin (R), pyrazinamide, ethambutol, and INH (H) daily for 2 mos. followed by rifampicin
Pentamidine, pneumocystosis, and pneumothorax.: Int Conf AIDS. 1991 Jun 16-21;7(1):226 (abstract no. M.B.2177). Unique Identifier : AIDSLINE ICA7/1217791; Coker R; Moss F; Peters B; Nieman R; Mitchell D; Harris J; Department of Genitourinary Medicine, St. Mary's Hospital,; London, UK; OBJECTIVE: To determine the risk of pneumothorax associated with Pneumocystis carinii pneumonia (PCP) and the role of aerosolised pentamidine in its aetiology. METHOD: 298 patients with AIDS treated at St. Mary s Hospital between Jan. 1989 and Jan. 1991 were studied. Episodes of pneumothorax were documented, and past
Treatment of pulmonary Kaposi's sarcoma (PKS) with combination chemotherapy.: Int Conf AIDS. 1991 Jun 16-21;7(1):226 (abstract no. M.B.2178). Unique Identifier : AIDSLINE ICA7/1217891; Sloand E; Kumar P; Pierce P; National Heart, Lung, and Blood Institute, Bethesda, Maryland; OBJECTIVE: To determine the effect of chemotherapy on survival in AIDS patients with PKS and compare it to the 3 mo median survival of historical controls. METHODS: 14 AIDS patients with PKS were treated with combination chemotherapy consisting of daunorubicin (20 mg/m(2)), bleomycin (15 units/m(2)), vinblastine (4mg/
Respiratory tract infections by bordetella bronchiseptica in AIDS.: Int Conf AIDS. 1991 Jun 16-21;7(1):226 (abstract no. M.B.2179). Unique Identifier : AIDSLINE ICA7/1217991; Amador C; Guitierrez F; Chiner E; Calpe JL; Ortiz de la Tabla V; Pasquau F; Vilar A; Hospital de Villajoyosa, Alicante, Spain; Bordetella bronchieseptica (BB) is a common veterinary pathogen. It has rarely been implicated in human infections and most of them occurred in immunocompromissed patients. However, there are not reports in the literature of infections in BB in AIDS patients. We present two cases of AIDS with respiratory infections by
Unsuspected pulmonary tuberculosis in patients with suspected pneumocystis carinii pneumonia (PCP).: Int Conf AIDS. 1991 Jun 16-21;7(1):227 (abstract no. M.B.2180). Unique Identifier : AIDSLINE ICA7/1218091; Klein RS; Motyl M; Montefiore Medical Ctr/Albert Einstein College of Medicine,; Bronx, NY, USA; OBJECTIVE: To determine the rate of unsuspected tuberculosis (TB) among patients suspected of having PCP, in a city with many cases of TB. METHODS: Patients having sputum induction for suspected PCP had specimens stained for P. carinii by direct immunofluorescence and for mycobacteria by Kinyoun stain and cultured for
Serodiagnosis of tuberculosis in HIV infected patients using ELISA and glycolipid antigens from mycobacterium tuberculosis.: Int Conf AIDS. 1991 Jun 16-21;7(1):227 (abstract no. M.B.2181). Unique Identifier : AIDSLINE ICA7/1218191; Berlie HC; David HL; Cruaud P; Papa F; Petit JC; Hopital Saint-Antoine, Paris, France; Sera from more than 50 tuberculous patients infected with the Human Immunodeficiency Virus (HIV) were tested by ELISA using the phenol glycolipid (PGL-Tb1) and the sulfolipid (SL-IV) antigens isolated from M tuberculosis. In all cases the diagnosis of tuberculosis was bacteriologically confirmed by isolation and ident
Symptomatic cytomegaloviral rhinosinusitis in patients with AIDS.: Int Conf AIDS. 1991 Jun 16-21;7(1):227 (abstract no. M.B.2182). Unique Identifier : AIDSLINE ICA7/1218291; Brillhart T; Gathe J Jr; Piot D; Stool E; Ussery F; Pope-Pegram L; Mabrie H; Foscarnet Research Study, Houston, Texas, USA; OBJECTIVE: To document and describe the first cases of cytomegaloviral rhinosinusitis in patients with AIDS. METHODS: Prospective follow up of two patients with biopsy proven cytomegaloviral rhinosinusitis. Appropriate x-rays, laboratory, and histopathology are available for review. RESULTS: (Patient 1) 41 year old ma
Use of interventions to prevent HIV associated lung infections.: Int Conf AIDS. 1991 Jun 16-21;7(1):227 (abstract no. M.B.2183). Unique Identifier : AIDSLINE ICA7/1218391; Glassroth J; Wallace J; Hopewell P; Jordan M; Northwestern U, Chicago, IL, USA; OBJECTIVE: To define the use of available interventions to prevent HIV associated lung infections by participants in a study of pulmonary complications of HIV. METHODS: Responses to a standardized questionnaire by 1369 participants at 6 U.S. Centers were analyzed for the use of preventive interventions. Subjects inclu
AIDS-related Kaposi's sarcoma: clinical and immunological follow-up 10 patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):228 (abstract no. M.B.2184). Unique Identifier : AIDSLINE ICA7/1218491; Brandini L; Mastrolorenzo A; Tiradritti L; Rapaccini AL; Zuccati G; Panconesi E; Department of SID, Institute of Dermatology, University of; Florence, Italy; The clinical course of AIDS-related Kaposi s sarcoma is profoundly variable depending on the subject. In order to assess if a close relationship exists between the clinical stages of the tumor and certain immunologic parameters, we studied the possibility of a correlation between the clinical types and the degree of i
Incidence of the isolation of yeasts in the oropharynx of HIV positive patients.: Int Conf AIDS. 1991 Jun 16-21;7(1):228 (abstract no. M.B.2185). Unique Identifier : AIDSLINE ICA7/1218591; Pestiglione A; Conte M; Galderisi P; Di Nicuolo G; Esposito C; Smeraglia R; Gnarini R; Ospedale D. Cotugno Naples - Italy; OBJECTIVE: our study aims at evaluating the incidence of yeasts presence in the oropharyngeal cavity of HIV positive patients and their association whith pathogenic bacteria. METHODS: 250 oropharingeal smears of as many patients hospitalized at Ospedale D. Cotugno of Naples from 7/9/88 to 31/12/90 have been examined.
Odansetron as an anti-emetic in AIDS patients receiving high dose co-trimoxazole (TMP-SMX): an open study.: Int Conf AIDS. 1991 Jun 16-21;7(1):228 (abstract no. M.B.2186). Unique Identifier : AIDSLINE ICA7/1218691; Main J; Gompels M; McWilliams S; Harris JR; Pinching AJ; Dept of Medicine, St. Mary's Hospital, London, UK; OBJECTIVE: To access the efficacy and tolerance of Odansetron, a novel anti-emetic agent, for TMP-SMX induced nausea and vomiting. METHOD: Subjects presenting with their first episode of Pneumocystis carinii pneumonia (PCP) were treated with Odansetron 8mg tds orally for 25 days and TMP-SMX (TMP 20 mg/Kg, SMX 100mg/Kg
Clinical risk situations for malnutrition and outcome of nutritional intervention in advanced HIV-disease.: Int Conf AIDS. 1991 Jun 16-21;7(1):228 (abstract no. M.B.2187). Unique Identifier : AIDSLINE ICA7/1218791; Schwenk J; Buerger B; Salzberger B; Factkenheuer G; Skorupka M; Ollenschlaeger G; Schrappe M; Nutrition and HIV-Infection Study Group, University of Cologne,; Germany; OBJECTIVE: To identify clinical risk situations in HIV infection, and to evaluate the therapeutic effect of intensified oral nutritional intervention (IONI) in severely ill and malnourished patients (pts). PATIENTS AND METHODS: From all 98 HIV-positive pts in our ongoing prospective study on IONI (11/89-1/91), 38 pts
Cutaneous side effects: a major problem in the treatment of toxoplasmosis encephalitis (TE).: Int Conf AIDS. 1991 Jun 16-21;7(1):229 (abstract no. M.B.2188). Unique Identifier : AIDSLINE ICA7/1218891; Annick G; Zamora L; Caumes E; Katlama C; Bricaire F; Gentilini M; Hopital Pitie-Salpetriere - 75013 PARIS, FRANCE; OBJECTIVE: To compare clinical and biological characteristics of cutaneous side effects (SE) of pyrimethemine/sulfadiazine (P/S) vs pyrimethamine/clindamy