KAPOSI SARCOMA LIKE CELLS (KSLC) OBTAINED FROM PERIPHERAL BLOOD OF PATIENTS WITH KAPOSI'S SARCOMA (MEETING ABSTRACT)

Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

KAPOSI SARCOMA LIKE CELLS (KSLC) OBTAINED FROM PERIPHERAL BLOOD OF PATIENTS WITH KAPOSI'S SARCOMA (MEETING ABSTRACT)

Proc Annu Meet Am Soc Clin Oncol; 10:A5 1991. Unique Identifier : AIDSLINE ICDB/91671337
Feigal EG; Looney DJ; Poeschla E; Badel P; Wong-Staal F; Univ. of California, San Diego Medical Center, La Jolla, CA

Abstract: Introduction: To determine the capacity of infected primary cells, plasmas, and culture supernatants from different patient (pt) populations to stimulate the growth of KS cells of KSLC lines in vitro, and to identify factors involved in such stimulation. To determine if KSLC can be isolated more frequently from the blood of pts with KS or at risk for KS. Methods: We cultured peripheral blood mononuclear cells (PBMC) from 23 pts: 2 HIV-KS+; 4 HIV+KS- hemophiliacs, and 17 HIV+KS+, compared to a control group of HIV-KS-pts. Samples of PBMC were frozen for PCR of cytokine MRNA. Results: KSLC were obtained from 0/2 HIV-KS+, 0/4 HIV+KS- hemophiliacs, and 7/19 HIV+KS+ pts, compared to 0 of HIV-KS- controls. IL-2, PHA, IL-6 containing supernatant from THP-1 LPS stimulated cells, HIV-2 conditioned media, HTLV-I TAX-containing supernatants, hydrocortisone, dexamethasone, heparin and EGF inhibited or failed to promote growth of KSLC when added directly to primary cultures. In contrast, supernatants from HIV-IL-2 treated PBMCs stimulated growth of KSLC in every case. Two continuous KSLC lines are negative for factor VIII, Fc receptors, leukocyte peroxidase, leukocyte alkaline phosphatase, alpha-chloronaphthyl esterase, CD4, CD3, and CD11, and strongly positive for vimentin and cytokeratin. Angiogenic potential of these cells is currently in progress. Preliminary results indicate that KSLC respond to supernatants from cells transfected with HIV TAT-producing plasmids with a 2- to 3-fold increase in proliferation. Comments: KSLC can be cultured from HIV+ pts with KS compared to HIV+ pts without KS, and HIV- controls. KSLC have a 2- to 3-fold increase in proliferation with supernatants transfected with TAT-producing plasmids. Cytokine production by PBMC and lesion-derived KSLC and their responsiveness to cytokines and mutant and wild-type TAT is under investigation.

Keywords: Cell Division/*PHYSIOLOGY Gene Products, tat/PHYSIOLOGY Human HIV Seropositivity/*PATHOLOGY *Neoplasm Circulating Cells Sarcoma, Kaposi's/*PATHOLOGY Tumor Cells, Cultured/*PATHOLOGY ABSTRACT

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