Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.
HUMAN HERPESVIRUS 6 STRAIN Z29: GROWTH CHARACTERISTICS, VIRION SPECIFIC PROTEIN IDENTIFICATION AND THE EFFECT OF INFECTION ON THE HOST
Diss Abstr Int [B]; 51(5):2199 1990. Unique Identifier : AIDSLINE ICDB/91669611 Black JB; Emory Univ.
Abstract:
Human herpesvirus 6 (HHV-6) was first isolated from the peripheral blood lymphocytes of patients with various lymphoproliferative disorders, including AIDS, in 1986. It has since been shown that greater than 95% of adults have serologic evidence of infection and that it is the causative agent of exanthem subitum. This thesis reports the first growth, cell tropism and virion component characterization of strain HHV-6(Z29), and the effect of infection with this virus on host cell proliferation and protein synthesis. Preliminary characterization of HHV-6(Z29) indicated that it was antigenically and genetically distinct from the other known human herpes viruses. The virus was found to be highly cell associated and T-cell tropic. Furthermore, this strain of HHV-6 grew poorly in tissue culture. Experiments were designed to optimize the growth conditions of HHV-6(Z29). The virus grows best in phytohemagglutin-stimulated human umbilical cord blood lymphocytes in media containing interleukin 2 and hydrocortisone. Once the virus adapted to growth in CBL higher titers of infectious virions were released into the cell culture supernatant. Methods were devised for purification of virions from the supernatant and for identification of virion proteins. Over 20 virion proteins were identified ranging in molecular weight from less than 30,000 to greater than 200,000. A 101 kD protein was found to be a specific serologic marker of HHV-6 infection by multiple criteria. The effect of infecting human umbilical cord blood lymphocytes with HHV-6(Z29) was examined. A large and unusual increase in host cell protein synthesis was noted in infected cultures relative to uninfected cultures. Experiments were designed to determine the stage of viral replication during which the factor(s) that mediates this effect is expressed. I found that the increase in protein synthesis in the infected cell cultures was primarily host protein and was dependent on a factor(s) whose expression is dependent on viral DNA replication. (Full text available from University Microfilms International, Ann Arbor, MI, as Order No. AAD90-27895)
Keywords: Acquired Immunodeficiency Syndrome/MICROBIOLOGY Cells, Cultured DNA Replication Herpesviridae Infections/MICROBIOLOGY Herpesvirus 6, Human/GROWTH & DEVELOPMENT/METABOLISM/*PHYSIOLOGY Human Molecular Weight T-Lymphocytes/MICROBIOLOGY Viral Proteins/*BIOSYNTHESIS Virion/GROWTH & DEVELOPMENT/METABOLISM/*PHYSIOLOGY Virus Replication THESIS 910430
M9140686
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Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.
