THE ROLE OF EARLY PROTEINS AND THE LATE REGION OF COTTONTAIL RABBIT PAPILLOMAVIRUS IN CELL TRANSFORMATION AND TUMOR INDUCTION.

Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

THE ROLE OF EARLY PROTEINS AND THE LATE REGION OF COTTONTAIL RABBIT PAPILLOMAVIRUS IN CELL TRANSFORMATION AND TUMOR INDUCTION.

Diss Abstr Int [B]; 51(7):3255 1991. Unique Identifier : AIDSLINE ICDB/91671012
Meyers CM; Univ. of California, Los Angeles

Abstract: Papillomas induced by the cottontail rabbit papillomavirus (CRPV) progress at a high frequency to cancers. This property makes the CRPV system unique for the study of tumorigenesis and carcinogenesis. In this dissertation we have looked at CRPV regulatory sequences and early gene products that are likely to be involved in tumor induction. Two types of regulatory sequences have been identified in the late region. One sequence a segment of about 300 bp located within ORF L2 inhibits transformation of fibroblasts (NIH 3T3) but does not affect transformation of rabbit skin epithelial cells (sf1Ep) and tumor induction in rabbits. The second sequence is located in the 3' half of ORF L1 and is absolutely required for transformation of epithelial cells and tumor induction but has only a quantitative effect on transformation of fibroblasts. These findings have permitted the establishment of systems for the genetic analyses of early CRPV genes. Expression of the long E6, E7, and E5 proteins are required for maximal transformation of NIH 3T3 and sf1Ep cells, and for efficient colony formation in soft agar. The short E6 protein, initiated at the second ATG of ORF E6 does not affect morphologic transformation of cells but has an effect on colony formation in soft agar. In contrast to CRPV transformed NIH 3T3 cells, CRPV transformed sf1Ep cells are not tumorigenic for nude mice and thus may provide a system to study the interaction of virus and carcinogens in the development of malignancies. Finally, expression of all four early proteins is required for tumor induction in rabbits. (Full text available from University Microfilms International, Ann Arbor, MI, as Order No. AAD90-33996).

Keywords: Animal Base Sequence Cell Transformation, Neoplastic/*GENETICS Cell Transformation, Viral/*GENETICS Genes, nef Mice Mice, Nude Oncogene Proteins, Viral/GENETICS Papilloma/*GENETICS/MICROBIOLOGY Papillomavirus/*GENETICS Rabbits Tumor Virus Infections/*GENETICS/MICROBIOLOGY THESIS
910430
M9140684

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