GENETIC ANALYSIS OF THE L1MD REPEAT ELEMENT AND THE HIV 1 POL GENE

Important note: Information in this article was accurate in 1990. The state of the art may have changed since the publication date.

GENETIC ANALYSIS OF THE L1MD REPEAT ELEMENT AND THE HIV 1 POL GENE

Diss Abstr Int [B]; 50(10):4380 1990. Unique Identifier : AIDSLINE ICDB/90665818
Loeb DD; Univ. of North Carolina, Chapel Hill

Abstract: The complete nucleotide sequence of a 6851 bp member of the L1Md repetitive family was determined. Five kb of the element contains two overlapping reading frames of 1,137 and 3,900 bp. The relative organization of these reading frames, which overlap by 14 bp, bear resemblance to protein-coding mobile genetic elements. This analogy is further supported by amino acid homology between regions in the 3900 bp frame and several reverse transcriptases. The 5' ends of two described L1Md elements have multiple copies, 4(2/3) copies and 1(2/3) copies, of a 208 bp direct tandem repeat. This 5' end sequence differs from a previously described 5' end sequence, indicating L1Md can have two different 5' end motifs. A heterologous expression system to study HIV 1 protease function has been developed. It involves the expression of the pol reading frame of HIV 1 under the control of an inducible promoter in E coli. The pol precursor protein is processed by the pol encoded protease to yield the mature pol derived products; the protease, the two forms of the reverse transcriptase, and the integrase. Using oligonucleotide-directed mutagenesis procedures we have constructed a residue by residue missense mutation map of the 99 amino acid HIV 1 protease. Three regions in which consecutive residues were sensitive to mutational inactivation were found. The relationship of these mutation sensitive regions to known functions of the protease will be discussed, along with several conditional mutants. (Full text available from University Microfilms International, Ann Arbor, MI, as Order No. AAD90-07296)

Keywords: DNA, Viral/*GENETICS *Genes, pol HIV-1/ENZYMOLOGY/*GENETICS Mutation Oligonucleotide Probes *Repetitive Sequences, Nucleic Acid RNA-Directed DNA Polymerase/GENETICS Sequence Homology, Nucleic Acid THESIS
901030
M90A0670

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