HIV DETECTION BY GENETIC ENGINEERING METHODS NLM AIDSLINE Important note: Information in this article was accurate in 1990. The state of the art may have changed since the publication date.

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HIV DETECTION BY GENETIC ENGINEERING METHODS

HIV Detection by Genetic Engineering Methods. Luciw PA and Steimer KS, eds. New York, Marcel Dekker, 301 p., 1989.. Unique Identifier : AIDSLINE ICDB/90660251
Anonymous; No affiliation given

Abstract: By early 1985, several isolates of HIV were molecularly cloned and completely sequenced. This made it possible to develop specific nucleic acid probes for HIV and to apply genetic engineering methodologies to the production of viral polypeptides in recombinant microorganisms. These events led to the development of sensitive assay systems for detecting the virus and for measuring immune responses to virus infection. Progress in molecular approaches to diagnosing HIV infection is reviewed under the following headings: diagnostic implications of the molecular features of HIV, epidemiology of AIDS, detection of HIV antibodies using recombinant envelope and core proteins, immunologic analysis of HIV-recombinant proteins and their use as reagents for serologic tests, recombinant antigens as diagnostic and screening reagents for HIV infections (experience with a commercial test), suitability of recombinant antigens and a synthetic peptide as HIV-diagnostic reagents, recombinant immunoblot assay for the detection of antibodies to HIV, immunoreactivity of AIDS/AIDS-related complex patient sera with synthetic oligopeptides from HIV gp41, an assay for the detection of HIV-seropositive individuals based on a chemically synthesized peptide, antigen assays for HIV, detection of HIV antigen(s), in situ hybridization as a histomolecular approach to the detection and study of HIV infection, diagnosis of HIV in seropositive individuals (enzymatic amplification of HIV viral sequences in peripheral blood mononuclear cells), genetic variation among isolates of HIV-1 and HIV-2, and diagnosis of human T-cell leukemia virus infection.
Keywords: *AIDS Serodiagnosis *Genetic Engineering Human HIV/*GENETICS HIV Infections/*DIAGNOSIS MONOGRAPHKWDaidsserodiagnosisKWDgeneticengineeringhumanhiv/KWDgeneticshivinfections/KWDdiagnosismonograph
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Copyright © 1990 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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