MOLECULAR FEATURES OF HIV--IMPLICATIONS FOR DIAGNOSTICS NLM AIDSLINE Important note: Information in this article was accurate in 1990. The state of the art may have changed since the publication date.

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MOLECULAR FEATURES OF HIV--IMPLICATIONS FOR DIAGNOSTICS

HIV Detection by Genetic Engineering Methods. Luciw PA and Steimer KS, eds. New York, Marcel Dekker, p. 1-14, 1989.. Unique Identifier : AIDSLINE ICDB/90660252
Luciw PA; Univ. of California at Davis, Davis, CA

Abstract: In early attempts to detect HIV, tissue culture procedures were used to recover virus from peripheral blood lymphocytes. Since the revelation of the genetic structures of HIV-1 and HIV-2 by molecular cloning and DNA sequencing, application of recombinant DNA technologies has led to the development of sensitive, specific, and rapid diagnostic systems for detecting viral nucleic acids, viral proteins, and antiviral antibodies. The molecular features of HIV structure and replication that are relevant to diagnostic procedures are reviewed, and investigations with implications for further developments in methods of detecting HIV components and antiviral immune response are discussed. Genetic organization of HIV, virion structure and assembly, regulation of HIV gene expression, and sequence variation are considered. The molecular methods of HIV detection can also provide critical information on the AIDS epidemic and on the mechanisms of viral pathogenesis. In addition, sensitive and specific diagnostic systems are essential for evaluating the efficacy of candidate HIV vaccines and for monitoring individuals undergoing chemotherapeutic regimens aimed at inhibiting HIV replication. (89 Refs)
Keywords: Base Sequence *Cloning, Molecular Gene Expression Regulation, Viral Genes, Viral Human HIV Infections/*DIAGNOSIS HIV-1/*GENETICS HIV-2/*GENETICS Virion/GENETICS MONOGRAPH REVIEW, TUTORIAL REVIEWKWDbasesequenceKWDcloning,moleculargeneexpressionregulation,viralgenes,viralhumanhivinfections/KWDdiagnosishiv-1/KWDgeneticshiv-2/KWDgeneticsvirion/geneticsmonographreview,tutorialreview
900530
M9051022

Copyright © 1990 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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