Safe and efficient ecotropic and amphotropic packaging lines for use in gene transfer experiments. NLM AIDSLINE Important note: Information in this article was accurate in 1990. The state of the art may have changed since the publication date.

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Safe and efficient ecotropic and amphotropic packaging lines for use in gene transfer experiments.

Trans Assoc Am Physicians. 1988;101:212-8. Unique Identifier : AIDSLINE MED/90070372
Markowitz DG; Goff SP; Bank A; Department of Genetics and Development, Columbia University,; College of Physicians and Surgeons, New York, New York 10032.

Abstract: The construction of a retrovirus packaging cell line which produces high-titer, helper-free retrovirus is an essential prerequisite for experiments whose goal is gene therapy. We have constructed an ecotropic packaging cell line, GP + E-86, and an amphotropic packaging cell line, GP + envAm12, in which the viral gag and pol genes are on one plasmid and the viral env gene is on another plasmid. Both plasmids contain deletions of the psi packaging sequence and the 3' LTR. The fragmented helper virus genomes, when introduced into 3T3 cells, produce titers of delta neo or N2 retrovirus which are comparable to titers produced from packaging cells containing the helper virus genome on a single plasmid. We have found no evidence for the generation of wild-type retrovirus using the GP + E-86 and GP + envAm12 packaging lines, either alone or in combination with the N2 retroviral vector. These results indicate that these packaging lines are safe and efficient for use in experiments designed for murine (using GP + E-86) and human (using GP + envAm12) gene therapy.
Keywords: Animal *Cell Line DNA, Recombinant DNA, Viral Gene Therapy Genes, env *Genetic Vectors Human Mice Plasmids Retroviridae/*GENETICS *Transfection JOURNAL ARTICLEKWDanimalKWDcelllinedna,recombinantdna,viralgenetherapygenes,envKWDgeneticvectorshumanmiceplasmidsretroviridae/KWDgeneticsKWDtransfectionjournalarticle
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M9030689

Copyright © 1990 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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