Live-cell immunofluorescence for detection of human immunodeficiency virus type 1 (HIV-1)-related antigens in peripheral blood mononuclear cells from HIV-1 seropositive individuals. NLM AIDSLINE Important note: Information in this article was accurate in 1990. The state of the art may have changed since the publication date.

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Live-cell immunofluorescence for detection of human immunodeficiency virus type 1 (HIV-1)-related antigens in peripheral blood mononuclear cells from HIV-1 seropositive individuals.

Microbiologica. 1989 Oct;12(4):281-9. Unique Identifier : AIDSLINE MED/90066063
Valensin PE; Zazzi M; Valassina M; Bianchi Bandinelli ML; Toti M; Almi P; Rubino M; Caruso A; Institute of Microbiology of the University, Siena, Italy.

Abstract: Live-cell indirect immunofluorescence was used to detect HIV-associated antigens in fresh uncultured peripheral blood mononuclear cells from 29 HIV-seropositive subjects. Conventional fixed-cell immunofluorescence and live-cell immunofluorescence were run in parallel on each sample using monoclonal antibodies directed against HIV gag and env gene products. Sera from the same patients were also tested for HIV antigen by a sandwich enzyme immunoassay. Results strongly indicate that nondenaturing live-cell immunofluorescence is much more sensitive for detection of HIV-associated antigens and may be useful in the diagnosis of HIV infection as well as in the study of pathogenesis.
Keywords: Antibodies, Monoclonal Blotting, Western Fluorescent Antibody Technique Human HIV Antigens/*ANALYSIS HIV Infections/*DIAGNOSIS HIV Seropositivity/*BLOOD/DIAGNOSIS/IMMUNOLOGY HIV-1/*IMMUNOLOGY Immunoenzyme Techniques Leukocytes, Mononuclear/*IMMUNOLOGY JOURNAL ARTICLEKWDantibodies,monoclonalblotting,westernfluorescentantibodytechniquehumanhivantigens/KWDanalysishivinfections/KWDdiagnosishivseropositivity/KWDblood/diagnosis/immunologyhiv-1/KWDimmunologyimmunoenzymetechniquesleukocytes,mononuclear/KWDimmunologyjournalarticle
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M9030347

Copyright © 1990 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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