Important note: Information in this article was accurate in 1990. The state of the art may have changed since the publication date.
THE JAAGSIEKTE RETROVIRUS: ISOLATION AND COMPARISON WITH A NEW SOUTH AFRICAN LENTIVIRUS
Diss Abstr Int [B]; 50(5):1923 1990. Unique Identifier : AIDSLINE ICDB/90660241 York DF; Univ. of Natal, South Africa
Abstract:
Jaagsiekte is a contagious cancer affecting the lungs of sheep. Although the etiological agent is Jaagsiekte retrovirus (JSRV), two other retroviruses--viz, South African maedi visna virus (SA OMVV) and a novel Bovine retrovirus (BRV)--have been associated with or implicated in the jaagsiekte disease complex. JSRV was partially purified from lung rinse material, its polypeptide composition was studied by gel electrophoresis and its morphology observed electron microscopically. Monoclonal antibodies were made against purified preparations of the virus and used in the development of JS-specific immunoassays. A cross reaction between JSRV and a polyclonal serum against Mason Pfizer monkey virus (MPMV) was confirmed and used in a Western blot technique to identify, monitor and differentiate JSRV from other viruses. During the study of JSRV another retrovirus, found to be a novel South African isolate of maedi visna virus (SA - OMVV I), was often found in purified JSRV preparations. Being a retrovirus it is also detected by the reverse transcriptase assay which was the only method used to assay and monitor for JSRV during the early stages of our work. Using a Western blot technique and sera against MVV and MPMV it was possible to simultaneously detect and differentiate JSRV from SA - OMVV I. A method was also developed whereby the two viruses could be separated from each other during purification. BRV is a novel virus which originated from bovine cells that had been co-cultivated with white blood cells from an ox suffering from malignant catarrhal fever. Three out of four sheep inoculated with BRV developed JS. A comparative study revealed that BRV was biochemically and morphologically quite different from JSRV. However, serum against MPMV cross-reacted with a 32-kD protein of BRV indicating a serological relationship between JSRV, MPMV and BRV. The possible role of BRV in the etiology of jaagsiekte remains to be elucidated.
Keywords: Animal Antigens, Viral/IMMUNOLOGY Cattle Comparative Study Cross Reactions Malignant Catarrh/MICROBIOLOGY Pulmonary Adenomatosis, Ovine/*MICROBIOLOGY Retroviridae/IMMUNOLOGY/*ISOLATION & PURIF Sheep South Africa SIV/ISOLATION & PURIF Visna-Maedi Virus/ISOLATION & PURIF THESIS 900630
M9060639
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Important note: Information in this article was accurate in 1990. The state of the art may have changed since the publication date.
