INFECTION OF TERMINALLY DIFFERENTIATED CHICKEN MYOTUBES AND CHICKEN EMBRYO FIBROBLASTS (CEF) WITH ROUS SARCOMA VIRUS (RSV)

Important note: Information in this article was accurate in 1990. The state of the art may have changed since the publication date.

INFECTION OF TERMINALLY DIFFERENTIATED CHICKEN MYOTUBES AND CHICKEN EMBRYO FIBROBLASTS (CEF) WITH ROUS SARCOMA VIRUS (RSV)

Diss Abstr Int [B]; 50(7):2772 1990. Unique Identifier : AIDSLINE ICDB/90662258
Park HT; Univ. of Pennsylvania

Abstract: It is established in our laboratory that in terminally differentiated chicken myotubes, where no chromosomal DNA replication takes place, RSV can infect and synthesize RSV-DNA. We have examined the synthesis of RSV mRNA and the particles produced from the late-infected myotubes (LIM) (defined as such, because they were infected at late stage of myotube development). The average ratio of RSV 38S (gag and pol) to 28S (env) to 21S (src) was comparable to CEF, as revealed by Northern analysis. SDS-PAGE of immunoprecipitate of [35S]-labeled viral particles showed comparable amount of gag protein but decreased amount of env protein for LIM compared to CEF. The ratio of focus forming unit to reverse transcriptase activity (FFU/RTase) of particles from LIM was lower than that of CEF. Microinjection of molecularly cloned RSV-DNA (SRA-2) into myotubes produced infectious particles and showed positive indirect immunofluorescence, suggesting that small amount of infectious particles observed in LIM culture could have come from the myotubes and not from the contaminating fibroblasts. In addition to LIM, the infected CEF on day 2 post infection (2pi) and 5pi were examined. FFU/RTase and env/gag ratios of particles from 2pi were less than that of 5pi. There was less cell-bound env protein in 2pi than in 5pi CEF. RNA analysis indicated that normal amount of 38S, 28S and 21S were synthesized. This was similar to what was observed in LIM. We conclude, therefore, differentiation of myotube affects the life cycle of RSV. Infected CEF at 2pi behaves similar to the late-infected myotube. (Full text available from University Microfilms International, Ann Arbor, MI, as Order No. AAD89-22581)

Keywords: Animal Blotting, Northern Cell Differentiation Chick Embryo Chickens Fibroblasts/MICROBIOLOGY Gene Products, env/GENETICS Gene Products, gag/GENETICS Muscles/CYTOLOGY RNA, Messenger/BIOSYNTHESIS RNA, Viral/BIOSYNTHESIS Sarcoma Viruses, Avian/*GENETICS/PHYSIOLOGY Virus Replication THESIS
900630
M9060637

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