Important note: Information in this article was accurate in 1990. The state of the art may have changed since the publication date.
FREQUENCY OF RECOMBINATION BETWEEN ONC GENE SEQUENCES IN RETROVIRUS VECTORS AND CELLULAR PROTO-ONC GENES
Diss Abstr Int [B]; 49(11):4696 1989. Unique Identifier : AIDSLINE ICDB/90658064 Phares WF; Univ. of California, Berkeley
Abstract:
Transformation by a molecularly cloned transformation-defective (td) mutant of Rous sarcoma virus (RSV) with an internal deletion in its onc gene, src, was studied both in chickens and in chicken embryo fibroblasts in tissue culture. Tumor formation (fibrosarcomas at the injection site in the wing, and internal tumors in muscle and organs) occurred in 25% of injected birds, and the latency varied from 37-98 days. From most of the tumors, transforming viruses were obtained with focus-forming activity of wild-type RSV, and an isogenic src gene. In addition, transforming viruses were obtained from cells infected in vitro. Structure of three independent isolates was very uniform; no differences in 23 cleavage sites of five different restriction endonucleases were seen, 11 of which sites were in the src sequences. The analysis indicated that these viruses were recombinants, in which the deleted src sequences were restored by homologous recombination with cellular proto-src. The appearance of recombinant virus in vitro was measured by fluctuation analysis of a parallel series of secondary tdRSV stocks, prepared from molecularly cloned virus further purified by end-point dilution. Under these conditions, the frequency of transduction of proto-src sequences was reproducible, and it varied from one transduction per 4.1 x 10(6) to 1.6 x 10(7) infectious events. Tumorigenesis by the weakly oncogenic PRCII, which carries an internal deletion of the fps portion of the onc gene, Delta gag-fps, was also examined. Mixed sarcoma and fibromyxosarcoma, or multinodular fibrosarcoma, formation at the site of injection in 67% of inoculated birds occurred within 23-40 days; no internal tumors were observed as long as 46 days after injection. Fujinami sarcoma virus and PRCII, each of which contains a nondeleted Delta gag-fps transforming gene, caused fibromyxosarcomas in 100% of inoculated birds in 10-14 days. Only transforming virus with the typical Delta gag-Delta fps genome of PRCII was obtained from PRCII-induced tumors; no evidence for restoration of the sequences deleted in fps was seen. (Full text available from University Microfilms International, Ann Arbor, MI, as Order No. AAD89-02238)
Keywords: Animal Base Sequence Chick Embryo Chickens Gene Products, gag/GENETICS *Genes, Viral Mutation *Proto-Oncogenes *Recombination, Genetic Retroviridae Proteins, Oncogenic/*GENETICS Sarcoma Viruses, Avian/*GENETICS Sarcoma, Avian/*GENETICS/MICROBIOLOGY THESIS 900228
M9020583
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Important note: Information in this article was accurate in 1990. The state of the art may have changed since the publication date.
