Important note: Information in this article was accurate in 1990. The state of the art may have changed since the publication date.
A NOVEL SYSTEM FOR GENERATING PRIMATE RECOMBINANT HYBRID VIRIONS
Diss Abstr Int [B]; 51(3):1129 1990. Unique Identifier : AIDSLINE ICDB/90668550 Wilson CA; George Washington Univ.
Abstract:
The host range of a retrovirus, as determined by the cell types and species that it may infect, is a critical component of viral pathogenicity. The interaction of a retrovirus, via the envelope, with a specific cellular receptor is one of the key factors which determine the viral host range. Retroviral gene transfer technology provides an interesting and useful method to study envelope-receptor interactions. Since the packaged genome is replication defective, the infectious particles produced from such helper cell lines can only infect a cell once and, therefore, are not pathogenic. In the absence of other viral genes, the specific contribution of the envelope to the host range can be studied. Moreover, by varying the envelope portion of a retroviral vector, it may be possible to map the different functional domains of viral envelope proteins. In this study, several recombinant plasmids encoding retroviral components were derived in order to generate novel recombinant hybrid virions. These hybrid virions are composed of a Moloney murine leukemia virus (MoMLV) core and genome surrounded by the envelope proteins from one of the following retroviruses: MoMLV, gibbon ape leukemia virus (GALV), strain SEATO, or human T cell leukemia virus, type I (HTLVI). Use of a retroviral recombinant genome containing a marker that can be selected or histochemically detected, such as the neomycin resistance gene or beta-galactosidase gene, provides a way to determine the presence and host range of hybrid virions generated by transfecting these plasmids into NIH3T3 cells, simply by assaying by histochemical staining or selecting for neomycin resistant colonies in infected cell populations. A battery of target cell lines were exposed to recombinant hybrid virions in order to determine the host range. These data demonstrate that the envelope is the critical determinant of host range for these retroviruses in all cells assayed. A stable cell line expressing the MoMLV gag and pol proteins, as well as a genome that can be packaged, has been developed. High titer hybrid virions bearing a variety of envelopes can now be generated upon transfection and subsequent expression of single plasmids encoding retroviral envelopes. This cell line should facilitate further studies of envelope function. (Full text available from University Microfilms International, Ann Arbor, MI, as Order No. AAD90-20322)
Keywords: Animal Cell Line Genes, gag Genes, pol Human HTLV-I/GENETICS Moloney Leukemia Virus/GENETICS Primates *Recombination, Genetic Retroviruses, Simian/*GENETICS *Transfection Viral Envelope Proteins/GENETICS Virion/*GENETICS THESIS 901230
M90C3718
AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.
Important note: Information in this article was accurate in 1990. The state of the art may have changed since the publication date.
