Detection of HIV-1 infected individuals in a seronegative high-risk population by polyclonal B cell activation in vitro. NLM AIDSLINE Important note: Information in this article was accurate in 1990. The state of the art may have changed since the publication date.

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Detection of HIV-1 infected individuals in a seronegative high-risk population by polyclonal B cell activation in vitro.

Int Conf AIDS. 1990 Jun 20-23;6(1):137 (abstract no. Th.A.13). Unique Identifier : AIDSLINE ICA6/10001390
Sell K; Yehuda-Cohen T; Slade B; Powell J; Villinger F; McClure H; Ansari A; Emory University Sch. of Med., Atlanta, GA, USA

Abstract: OBJECTIVE: To determine if polyclonal activation of B cells in vitro leads to the secretion of anti HIV-1 reactive antibodies. METHODS: PBMC from a total of 215 individuals from a high-risk patient population at Grady Memorial Hospital were cultured in vitro with pokeweed mitogen (PWM) for 5 days. Serum and supernatant fluids (S.F.) were analyzed for anti HIV-1 reactive antibodies, using the HIV-1 Abbott ELISA kit and the Dupont Western blot kit. RESULTS: Of the 165 HIV-1 seronegative samples, 30 were found to secrete significant HIV-1 antibody which mostly reacted with HIV-1 env. A high frequency of DNA samples from these 30 individuals was also found to be HIV-1 positive by PCR. CONCLUSION: These data suggest that in addition to PCR techniques, polyclonal activation in vitro with PWM provides a simple assay for the detection of occult HIV-1 infection. The immunological and epidemiological implications of this study are readily apparent. TABULAR DATA, SEE ABSTRACT VOLUME.
Keywords: B-Lymphocytes/*IMMUNOLOGY Blotting, Western DNA, Viral/ANALYSIS Enzyme-Linked Immunosorbent Assay Human HIV Antibodies/*BIOSYNTHESIS HIV Infections/*DIAGNOSIS HIV-1/GENETICS/*IMMUNOLOGY *Lymphocyte Transformation Pokeweed Mitogens Polymerase Chain Reaction Risk Factors ABSTRACTKWDb-lymphocytes/KWDimmunologyblotting,westerndna,viral/analysisenzyme-linkedimmunosorbentassayhumanhivantibodies/KWDbiosynthesishivinfections/KWDdiagnosishiv-1/genetics/KWDimmunologyKWDlymphocytetransformationpokeweedmitogenspolymerasechainreactionriskfactorsabstract
901230
M90C3705

Copyright © 1990 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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