Early detection of HIV infection by PCR before seroconversion in multitransfused seronegative hemophiliacs and sexual partners.

Important note: Information in this article was accurate in 1990. The state of the art may have changed since the publication date.

Early detection of HIV infection by PCR before seroconversion in multitransfused seronegative hemophiliacs and sexual partners.

Int Conf AIDS. 1990 Jun 20-23;6(1):137 (abstract no. Th.A.14). Unique Identifier : AIDSLINE ICA6/10001490
Moncany ML; Berthier A; Ferris S; Montagnier L; Unite d'Oncologie Virale, Institut Pasteur, Paris, France

Abstract: Limitations of the available serological tests led us to study by PCR a heterogenous at risk population composed of paradoxical seronegative hemophiliacs and of seronegative partners of seropositive patients. We designed a complete set of primers covering the entire genomes of the HIV1 and of the HIV2. They were tested for their specificity and their efficiency and only 36 of them are currently used for our studies concerning the gag, pol, env, nef1, nef2, vif1, vif2, vpr, vpx, and vpu genes. The first study included four patients who have now seroconverted, the lymphocytes of whom being harvested during their seronegative period. Three of them are hemophiliacs, the fourth one being a partner of a seropositive hemophiliac. By PCR analysis, we could reproducibly detect the nef1, the vpr and the vpu genes, but not the complete set of HIV genes. This detection was performed one to three years before seroconversion of the patients. We obtained similar data for the hemophiliacs remaining seronegative and for the partners of seropositives: the nef, vpr and vpu genes were again more detected than the gag, pol and env genes. Computation of all the 35 patients indicates the following respective percentages for the gene detection: 77% for nef1, 77% for vpr and 74% for vpu (or 85% for nef1 + nef2, 76% for vpx + vpu and 77% for vpr). Control experiments with in-vitro HIV infected lymphocytes indicate that all genes could be detected by our PCR technique. These data, correlated to the clinical/immunological state, are open to several interpretations which will be discussed.

Keywords: Blood Transfusion Female Hemophilia/*COMPLICATIONS Human HIV Infections/COMPLICATIONS/*DIAGNOSIS HIV-1/GENETICS HIV-2/GENETICS Male *Polymerase Chain Reaction Reproducibility of Results *Sexual Partners ABSTRACT
901230
M90C3704

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