Mixed HIV-1/HIV-2 infection in Brazil confirmed by PCR analysis.

Important note: Information in this article was accurate in 1990. The state of the art may have changed since the publication date.

Mixed HIV-1/HIV-2 infection in Brazil confirmed by PCR analysis.

Int Conf AIDS. 1990 Jun 20-23;6(1):137 (abstract no. Th.A.16). Unique Identifier : AIDSLINE ICA6/10001690
Peralta JM; Ferreira JA; Pieniazek D; Krebs JW; Sion FS; Ferrera Ramos Filho C; Morais de Sa CA; Weniger BG; Heyward WL; Ou CY; et al; Fundacao Oswaldo Cruz, Rio de Janeiro (RJ), Brazil

Abstract: OBJECTIVE: Analysis of sera from Brazil in 1989 suggested the presence of HIV-1, -2, and mixed infections within the populace. To further evaluate these observations HIV-1 and -2 PCR analysis was performed on selected sero-reactive persons. METHODS: Peripheral blood mononuclear cells were cultured and HIV proviral sequences amplified by PCR using type-specific primers and probes from the LTR, gag, or protease gene regions. Amplified HIV protease sequences from mixed infections were endonuclease digested and compared to established HIV isolates. RESULTS: Preliminary serological findings for the Brazilian samples were confirmed using independent sets of primers and probes. Endonuclease digests from mixed infections demonstrated both HIV-1 and -2 sequences that were distinct from other Brazilian specimens as well as the HIV controls. CONCLUSIONS: A combination of culture and PCR findings demonstrated the presence of both HIV-1 and -2 in Brazil. Restriction enzyme analysis of PCR products confirmed mixed HIV-1/-2 infection in 2 Brazilians.

Keywords: Brazil/EPIDEMIOLOGY DNA, Viral/*ANALYSIS Human HIV Infections/*DIAGNOSIS/EPIDEMIOLOGY HIV-1/*GENETICS HIV-2/*GENETICS *Polymerase Chain Reaction Restriction Mapping ABSTRACT
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M90C3702

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