Outlines objectives of bone marrow transplantation in a HIV-1 infected patient: to treat the underlying disease; to eradicate HIV-1 infected cells; to inhibit infection of donor cells.
Discusses the incidence of pruritic eruptions in a San Francisco population study composed of homosexual men and IV drug users. Reviews dermatologic disorders frequently seen in this population.
Asserts that carefully controlled serum glucose levels during pregnancy can dramatically decrease morbidity and mortality among women with gestational diabetes and their babies. Suggests early diagnosis of high risk patients.
Garden Grove, CA : Medcom/Trainex, c1989 7 videocassettes : sd., col. ; 1/2 in. + 3 guides + 1 workbook Unique Identifier : AIDSLINE AV/9000808A
Neu HC
(Producer) Meets OSHA requirements for CDC training and provides documentation for OSHA inspections. Designed to teach all employees (housekeeping, maintenance, technicians, nursing assistants, nurses, and doctors) how to protect themselves from being exposed to HBV and HIV infection at work.
San Francisco, CA : Visiting Nurses and Hospice of San Francisco, c1989 1 videocassette (ca. 35 min.) : sd., col. ; 1/2 in Unique Identifier : AIDSLINE AV/9000813A
Clark J; Martin JP; Schietinger H
(Producer) Using actual patient and staff interventions, this video helps nurses and other caregivers to recognize the physical and psychosocial complications associated with HIV infection. It describes the continuum of disability and necessary interventions through narration and interviews with nurses, social worke
Reviews the CDC universal precautions in handling blood and body fluids. Discusses the administrative decisions when dealing with a health care worker who is HIV positive while maintaining confidentiality.
Discusses the development of vaccines and the use of subunit vaccines. Explains how the unique properties of HIV make a vaccine difficult ; protocols and strategies used in developing and testing an HIV vaccine.
San Francisco, CA (Box 0884, San Francisco 94143-0884) : AIDS Health Project, University of California, San Francisco, c1987 100 p Unique Identifier : AIDSLINE CAT/9008269
Stuttgart : F. Steiner, 1987 14 p (Sitzungsberichte der Wissenschaftlichen Gesellschaft an der Johann Wolfgang Goethe-Universitat Frankfurt am Main ; Bd. 23, Nr. 4) Unique Identifier : AIDSLINE CAT/9008274
[San Francisco] (Box 0884, San Francisco 94143-0884) : AIDS Health Project, University of California, San Francisco, c1987 xii, 299 p Unique Identifier : AIDSLINE CAT/9009931
Washington, D.C. : U.S. Dept. of Justice, National Institute of Justice, Office of Communication and Research Utilization, 1988 xvii, 286 p. : ill (Issues and practices in criminal justice) Unique Identifier : AIDSLINE CAT/9008271
Oxford ; Boston : Blackwell Scientific Publications ; Chicago, Ill. : Distributors, USA, Year Book Medical Publishers, 1990 xi, 144 p. : ill Unique Identifier : AIDSLINE CAT/9008270
Amsterdam ; New York : Elsevier ; New York, NY, U.S.A. : Distributors for the U.S. and Canada, Elsevier Science Pub. Co., 1990 x, 371 p. : ill (Pharmacochemistry library ; v. 14) Unique Identifier : AIDSLINE CAT/9009276
This study concerns 15 together H.I.V. positive and psoriatic patients. The existing relationship between psoriasis and H.I.V. infection associated in the same patient still remains quite unclear. However, some conclusions may be drawn from those studies devoted to the association psoriasis-H.I.V. infection. Psoriasis
Bull World Health Organ. 1989;67(6):613-8. Unique Identifier : AIDSLINE MED/90227785
Many plant products are being used by patients with acquired immunodeficiency syndrome (AIDS) in some countries without any scientific proof that they possess anti-HIV (human immunodeficiency virus) activity. Traditional healers are now offering their remedies for scientific evaluation, and a few studies provide infor
Puccioni M; Favoreto AC; Andre C; Peixoto CA; Novis SA; Hospital Universitario Clementino Fraga Filho da Universidade; Federal do Rio de Janeiro, Brasil.
We studied the clinical records of 280 patients admitted to our Hospital between 1985 and 1988, with a positive Elisa test for HIV-related antibodies: 15.71% (44) of these patients exhibited clinical abnormalities related to disease of the CNS. In 6 (13.6%) patients the neurological complication was the first manifest
Bianchi S; Scaranari V; Tomatis R; Dipartimento Scienze Farmaceutiche, Universita di Ferrara,; Italy.
The cyclo [Thr-Thr-Thr-Tyr-Asn-Thr] hexapeptide related to peptide T, H-Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr-OH, competitor of the Human Immunodeficiency Virus in the binding to human T cells, was synthesized and tested for its ability to stimulate monocyte migration (chemotaxis). The new cyclic derivative showed negligibl
Ozel M; Hoglund S; Gelderblom HR; Morein B; Robert Koch-Institute of the Federal Health Office, Berlin,; Federal Republic of Germany.
Proteins of either HIV-1, hepatitis B, or rabies virus were incorporated with the adjuvant substance Quil A and cholesterol into the immunostimulating complex: iscom. Formation and symmetry of this regular complex were analyzed by electron microscopy. Micellar structures with a diameter of about 12 nm, occasionally wi
Av Odontoestomatol. 1989 May;5(5):293-6. Unique Identifier : AIDSLINE MED/90241363
Llanes F; Lopez G; Asenjo JA; Martin C
We present two cases of AIDS in drug-addicts, with mouth lymphoma. The diagnosis was established by a gum biopsy which gave high-grade non-Hodgkin lymphoma lymphoblastic B type. It is very rare that the oral mucous is affected by lymphomas, but in AIDS and immunodeficiencies it is more common.
118 patients with HIV infection underwent complete ophthalmologic examination to detect the presence of any eye pathology. The study was intended to show a correlation between such pathology and the total CD4 + lymphocyte count considered to be indicative of the immunologic status. The most frequently encountered lesi
Villanacci V; Favret M; Marocolo D; Mangiarini M; Grigolato P; Cestari R; Ravelli P
The symptoms of the Acquired Immuno-Deficiency Syndrome (A.I.D.S.) in the gastrointestinal tract are principally represented by neoplastic processes (Kaposi s sarcoma, lymphoma) and by opportunistic infections. These infections, for the diversity of bacterial, viral or parasitic etiological agents which are involved,
Cell-mediated immunity plays a pivotal role in the pathogenesis and in the recovery mechanisms of visceral leishmaniasis (V.L.). This disease, observed in two patients with AIDS, has peculiar anatomical and clinical characteristics and it is usually characterized by a severe clinical course. In addition, V.L. has been
Actual Odontostomatol (Paris). 1989 Jun;42(166):287-93. Unique Identifier : AIDSLINE MED/90247160
Soulhiard F; Beziat JL; Vitrey D; Nove-Josserand R
The authors report of a case of voluminous palate Kaposi s Sarcoma in a patient with A.I.D.S. Oral Kaposi s Sarcoma occurs in a large percentage of patients with A.I.D.S. (30 to 50%). The therapeutic of this voluminous Kaposi s Sarcoma is not easy. Surgery is the best therapeutic it can bring, as in this case, functio
Vago L; Antonacci MC; Cristina S; Parravicini C; Lazzarin A; Moroni M; Negri C; Uberti-Foppa C; Musicco M; Costanzi G; Servizio di Anatomia patologica dell'Ospedale Sacco,; Convenzionato con la V Cattedra dell'Universita di Milano,; Italia.
Morphological changes in lymph node biopsies of HIV-infected patients can be classified in four stages, based upon the degree of damage to follicular structures: (1) follicular hyperplasia, (2) follicular lysis, (3) follicular atrophy and (4) follicular and lymphocytic depletion. To define the relative usefulness of m
This paper reports a case of AIDS whose first symptom was SAE. The main clinic characteristics were epilepsy, and brain atrophy. In addition, there were also other features in this case, such as multiorgan damage, weight loss, fever and diarrhea. The patient s serum showed a positive result to anti HTLV-III, and TH:Ts
One thousand nine hundred and fifty-five pupils age 15 from Zagreb, Petrinja and the area of Zadar were tested in order to ascertain their knowledge on AIDS. The results obtained revealed that all the pupils tested are equally well informed about AIDS and that there is no statistically significant difference in that r
Plucne Bolesti. 1989 Jul-Dec;41(3-4):133-6. Unique Identifier : AIDSLINE MED/90260048
Vidmar L; Tomazic J
The problem of tuberculosis (TB) in population, infected by human immunodeficiency virus I (HIV I) in Slovenia is presented in the period from 1986 when the first patient was registered, till 1989. In Slovenia it has been established that TB plays an important role in patients wi
At present the increased incidence of infections with mycobacteria other than tuberculosis and leprae bacilli seems to be correlated with several causes: improved diagnostic techniques, prolonged life expectancy, immunodepression. Rational chemotherapy depends upon the identification of the etiologic mycobacterium and
Advances in biomedical technology have contributed effectively to the resolution of basic and clinical problems in Nephrology. Most of our insights on glomerular diseases come from animal models. Antibodies against components of the extracellular matrix have been shown to induce glomerular changes in vivo and the non-
Ferreira A; Silva Junior BA; Braga FM; Gargiulo NM; Stavale JN; Hospital Municipal Arthur Ribeiro de Saboya, Sao Paulo, Brasil.
A case of a 23 year-old-man with a history of polyarthritis and chronic tophaceous gout is reported. He developed a crural paraparesis caused by sodium urate deposits in the spinal canal. Decompressive laminectomy and removal of urate-laden ligamentum flavum was performed without improvement. According to the literatu
Gendelman HE; Meltzer MS; Henry M. Jackson Foundation for the Advancement of Military; Medicine, Rockville, Maryland.
Macrophages are an important in vivo reservoir for HIV. Conclusive evidence that CNS, pulmonary, lymph node and blood-derived mononuclear phagocytes harbor and support HIV replication is supported by numerous independent studies. HIV variants which preferentially replicate in macrophages have been recovered from infec
Rev Paul Med. 1989 May-Jun;107(3):169-74. Unique Identifier : AIDSLINE MED/90239452
Spina-Franca A
Considering data on cerebrospinal fluid (CSF) composition in infectious diseases of the central nervous system (CNS), an evaluation of the pathogeny of CNS infections is made. Basic factors considered in the evaluation are those related to the blood-brain barrier and to immune phenomena. The analysis supports the dist
Med Cutan Ibero Lat Am. 1989;17(6):409-13. Unique Identifier : AIDSLINE MED/90244827
Viglioglia PA; Rodriguez E; Carril A; Dermatologia de la Universidad de Buenos Aires, Argentina.
At present HL: should be considered a marker of immunodeficiency, though not necessarily associated with HIV infection. In eight patients with HL it was confirmed that this lesion, in male homosexuals, in a precursor and marker of AIDS. The diagnosis of HL warrant all efforts to protect the immune system and avoid pro
JPMA J Pak Med Assoc. 1989 Dec;39(12):312-6. Unique Identifier : AIDSLINE MED/90230490
Sonawalla AB; Lance JW; Lloyd A
Neurological disorders were studied in 18 patients diagnosed to have AIDS and their findings are analysed. Amongst the problems seen were toxoplasmosis (9), cryptococcal meningitis (5), tuberculous meningitis (1), AIDS dementia complex (3),
Spertzel RO; National Institutes of Health, Bethesda, MD 20892.
The search for a model of HIV infection continues. While much of the initial work focussed on animal models of AIDS, more recent efforts have sought animal models of HIV infection in which one or more signs of AIDS may be reproduced. Most initial small animal modelling efforts were negative and many such efforts remai
The concentrations of seven proteins [albumin (Alb), immunoglobulins (IgG, A, M), lactoferrin (Lf), lysozyme (Lzm) and beta 2-microglobulin (beta 2 m)] were measured in the tears and serum of 10 patients with acquired immune deficiency syndrome (AIDS) and in 13 normal subjects. In the AIDS group, alteration of the blo
Pediatr Med Chir. 1989 Nov-Dec;11(6):607-15. Unique Identifier : AIDSLINE MED/90259824
Stucchi C; Giovanniello A; Dozzi M; Careddu P; Carnelli V; Clinica Pediatrica I dell'Universita di Milano, Italia.
Thanks to recent developments and evolution in prenatal diagnosis and early onset within the first year of life, hemophilia may now be considered a pathology of primarily pediatric interest. The treatment of hemophilia in children has furthermore undergone a number of changes that include 2 main events in therapy that
The Authors propose a revision of the most recently literature on the Acquired Immune Deficiency Syndrome (AIDS), by stopping on ethiologic aspects and on the transmission modalities of HIV virus for the purpose to observe some precautional normes adoptable in odontoiatric practise.
AMB Rev Assoc Med Bras. 1989 May-Jun;35(3):111-6. Unique Identifier : AIDSLINE MED/90232181
Brigido LF; Duarte AJ
Infection by HIV usually leads to dysfunction of the immune system which, facilitates the development of opportunistic infections or neoplasia in which almost always are fatal. The pathological process associated with this infection involves, characteristically, the destruction or malfunction of helper T lymphocytes (
Ippolito F; Passi S; Caprilli F; Di Carlo A; Morrone A; Picardo M; Valenzano L
Seventy-six patients diagnosed as having seborrheic dermatitis (SD) were divided into two groups: group A (n = 22) otherwise healthy subjects (HIV negative) and group B (n = 54) HIV positive (ARC and AIDS cases). Thirty normal healthy subjects without SD were considered as control (group C). The three groups were subj
Hepatitis B remains a significant risk to patients and staff members of hemodialysis unit. Prior to immunization, 38 hemodialysis patients and 24 staff members were screened for HBs antigen, HBc antibodies and HBs antibodies using ELISA method (Abbott). Of 18 (47%) patients, five were chronic carriers of HBsAg and 13
Therizol-Ferly PM; Tagliante-Saracino J; Kone M; Konan A; Ouhon J; Assoumou A; Aka K; Assale G; Laboratoire de Parasitologie-Mycologie, CHU de Cocody.
148 adult patients with chronic diarrhoea and suspected to be HIV infected have had stool examinations. 46 are without any enteric parasite. Those detected in the others patients are Sporozoans: I. belli (16.2%) and Cryptosporidium sp. (6.7%) found alone or joint, together or with other parasites. Among those, all Fla
Funamoto K; Takada K; Inoue K; Sawada Y; Araga S; Takahashi K; Division of Neurology, Institute of Neurological Sciences,; Yonago, Japan.
A patient came to our clinic suffering from mild sensory loss, exaggeration in knee reflexes, bilateral extensor plantar responses and elevation of the antibody level to the human T-lymphotropic virus type I (HTLV-I). Electrophysiological and pathological studies showed axonal and demyelinating neuropathy. The conditi
Narayan O; Johns Hopkins University School of Medicine, Division of; Comparative Medicine, Baltimore, Maryland.
The immunopathogenesis of lentiviral lesions in sheep and goats requires continuous replication of the virus in tissues of the animal. This entails escape from various defense mechanisms of the host. Viral expression occurs mainly in tissue-specific macrophage populations and viral proteins produced by the cells induc
Castro LH; Chaves CJ; Callegaro D; Nobrega JP; Scaff M; Departamento de Neurologia, Faculdade de Medicina da Universidade; de Sao Paulo, Brasil.
In this preliminary report the authors present the finding of a high prevalence (37.5%) of seropositivity of antibodies to HTLV-I tested by Western blotting in a sample of 16 Brazilian patients with chronic myelopathies of undetermined origin.
Tao PZ; Johansson NG; Stening G; Oberg B; Datema R; Department of Virology, Chinese Academy of Medical Sciences,; Bejing.
The 5 -triphosphates of some 5-substituted 2 -deoxyuridine analogs were investigated for their effects on purified recombinant reverse transcriptase of human immunodeficiency virus type 1 (HIV-1) as well as cellular DNA polymerase alpha. The triphosphates were competitive inhibitors of the viral enzyme with dTTP as th
Ann Ig. 1989 Sep-Oct;1(5):1057-66. Unique Identifier : AIDSLINE MED/90234336
Sansone R; Carobbi S; Alloro G; Strigini P
While AIDS (Acquired Immunodeficiency Syndrome) certainly represents a worldwide health problem, the attention of many researchers and epidemiologists, besides the WHO itself, has recently focused on Africa for the following reasons: 1) The etiologic agent of AIDS, the Human Immunodeficiency Virus (HIV) (previously na
Bergamini L; Durelli L; Istituto di Clinica delle Malattie del Sistema Nervoso,; Universita Torino.
Animal models indicate that chronic relapsing demyelinating diseases of the central nervous system (CNS) can be induced both by certain viruses and by sensitization to myelinic antigens. Epidemiological data (studies on emigrants and on so-called Multiple Sclerosis (MS) epidemics) suggest that an environmental transmi
Albert J; Abrahamsson B; Nagy K; Aurelius E; Gaines H; Nystrom G; Fenyo EM; Department of Virology, National Bacteriological Laboratory,; Stockholm, Sweden.
The kinetics of appearance and specificity of HIV-1 neutralizing antibodies was studied in four individuals. HIV-1 was isolated during symptomatic primary HIV-1 infection and repeatedly thereafter, and tested against autologous sera collected in parallel. Our patients developed isolate-specific low-titer neutralizing
Wilks D; Walker LC; Habeshaw JA; Youle M; Gazzard B; Dalgleish AG; Division of Immunology, Clinical Research Centre, Harrow, UK.
The cell-surface antigen CD4 is the major receptor for HIV. Anti-CD4 autoantibodies and anti-idiotypic antibodies to murine monoclonal anti-CD4 antibodies have been described in HIV-infected people. Ninety-seven sera from HIV-infected people at all stages of disease were examined for the presence of anti-idiotypic ant
von Wussow P; Jakschies D; Block B; Tschechne B; Schedel I; Horisberger MA; Hochkeppel HK; Deicher H; Department of Immunology and Transfusion Medicine, Medical School; of Hannover, Federal Republic of Germany.
Twenty-six people with symptomatic HIV-1 infection were screened for the presence of interferon (IFN) alpha and IFN alpha antibodies in their sera and the presence of the IFN-induced intracellular Mx-homologous protein in their peripheral blood leukocytes. Eleven people had measurable IFN alpha levels ranging from 1 t
Blaxhult A; Granath F; Lidman K; Giesecke J; Department of Infectious Diseases, Karolinska Institute, Danderyd; Hospital, Stockholm, Sweden.
We collected information on the development of clinical signs and laboratory results from a cohort of 58 people infected with HIV through blood transfusion. The observation time ranged from 36 to 100 months. Belonging to the older age group at the time of transfusion was found to be an important factor for rapid progr
Simonsen JN; Plummer FA; Ngugi EN; Black C; Kreiss JK; Gakinya MN; Waiyaki P; D'Costa LJ; Ndinya-Achola JO; Piot P; et al; Centre for Microbiologic Research, Kenya Medical Research; Institute.
A cohort of 418 lower socioeconomic strata prostitutes were enrolled in a study of the epidemiology of sexually transmitted diseases (STDs) between January and April 1985. Sixty-two per cent of the women were seropositive for HIV infection at enrollment. Significant associations were found between HIV seropositivity a
Perry SW; Jacobsberg LB; Fishman B; Weiler PH; Gold JW; Frances AJ; Cornell University Medical College--New York Hospital, New York.
To determine the emotional impact of serological testing for HIV, 218 physically asymptomatic adults were evaluated in a confidential clinical setting 2 weeks before HIV test notification, immediately before and after notification, and 2 and 10 weeks later. All received extensive pre- and post-test counseling. The 179
Kall KI; Olin RG; Department of Psychiatry, Karolinska Institute, St Gorans; Hospital, Stockholm, Sweden.
An HIV study initiated in January 1987 is being conducted at the Remand Prison in Stockholm, Sweden , in order to follow HIV seroprevalence and HIV risk behaviour among intravenous drug users (IVDUs) in Stockholm. Up to December 1988 1152 arrested and detained IVDUs were interviewed
AIDS Res Hum Retroviruses. 1990 Feb;6(2):183-91. Unique Identifier : AIDSLINE MED/90226888
Morgan RA; Looney DJ; Muenchau DD; Wong-Staal F; Gallo RC; Anderson WF; Laboratory of molecular Hematology, National Heart, Lung, and; Blood Institute, National Institutes of Health, Bethesda, MD.
Retroviral vectors have been developed which produce a secreted form of the helper/inducer T-cell antigen, CD4. Amphotropically packaged vectors were used to transduce cells, and these cells were shown to express the secreted CD4 (sCD4) gene product. The sCD4 produced by the viral vectors is immunoprecipitated by mono
AIDS Res Hum Retroviruses. 1990 Feb;6(2):193-203. Unique Identifier : AIDSLINE MED/90226889
Matsushita S; Koito A; Maeda Y; Hattori T; Takatsuki K; Blood Transfusion Service, Kumamoto University Medical School,; Japan.
Either ricin A chain (RAC) or Pseudomonas exotoxin (PE) was conjugated with a murine monoclonal antibody (0.5 beta) directed against an external envelope glycoprotein (gp120) of human immunodeficiency virus (HIV). Effects of the immunotoxins produced against infected cells were evaluated. Selective inhibition of the p
AIDS Res Hum Retroviruses. 1990 Feb;6(2):219-28. Unique Identifier : AIDSLINE MED/90226891
Boudinot FD; Schinazi RF; Gallo JM; McClure HM; Anderson DC; Doshi KJ; Kambhampathi PC; Chu CK; Department of Pharmaceutics, and Medicinal Chemistry and; Pharmacognosy, College of Pharmacy, University of Georgia,; Athens.
The pharmacokinetics of the anti-human immunodeficiency virus type 1 nucleosides, 3 -azido-2 ,3 -dideoxyuridine (AzddU) and 3 -azido-3 -deoxythymidine ( AZT ) were characterized in rhesus monkeys. Half-life, total clearance, and steady-state volume of d
Eisenberg D; Wesson M; Molecular Biology Institute, University of California, Los; Angeles 90024.
A search for highly amphiphilic alpha-helices has been made in a data base of protein sequences, using the helical hydrophobic moment as a criterion of amphiphilicity. The protein segments of largest hydrophobic moment have been analyzed. For the segments whose structures are known, they are in fact alpha-helices. Two
Can Assoc Radiol J. 1990 Apr;41(2):100-2. Unique Identifier : AIDSLINE MED/90227473
Mirich D; Gray R; Hyland R; Department of Diagnostic Radiology, Wellesley Hospital, Toronto,; Ontario.
Pneumonia due to Legionella has been identified in a small percentage of patients suffering from acquired immune deficiency syndrome (AIDS), with and without coexisting Pneumocystis carinii pneumonia. On chest radiographs Legionella typically produces focal parenchymal opacities and confluent lobar consolidation. The
Can Assoc Radiol J. 1990 Apr;41(2):83-6. Unique Identifier : AIDSLINE MED/90227486
Harrison PB; Clarke SD; Silver SF; Department of Radiology, St. Paul's Hospital, Vancouver, British; Columbia.
Neurologic involvement is common in patients with acquired immune deficiency syndrome (AIDS). To assess the computed tomographic findings in AIDS, the authors reviewed 12 consecutive patients found to have focal brain lesions representing AIDS complications on computed tomography and having histologic or clinical proo
J Chromatogr. 1990 Feb 2;500:681-96. Unique Identifier : AIDSLINE MED/90229479
Righetti PG; Wenisch E; Jungbauer A; Katinger H; Faupel M; Chair of Biochemistry, University of Milan, Italy.
The performance of a multi-compartment electrolyser with isoelectric Immobiline membranes for large-scale protein purification is evaluated. Owing to the presence of isoelectric membranes possessing a high buffering capacity and ionic strength, isoelectric protein precipitation inside the membranes, one of the major d
J Immunol. 1990 May 1;144(9):3375-84. Unique Identifier : AIDSLINE MED/90229724
Tyler DS; Stanley SD; Nastala CA; Austin AA; Bartlett JA; Stine KC; Lyerly HK; Bolognesi DP; Weinhold KJ; Department of Surgery, Duke University Medical Center, Durham NC; 27710.
HIV-1-specific cell-mediated cytotoxicity (CMC) is a form of antibody-dependent cellular cytotoxicity (ADCC) in which HIV-1-specific antibodies arm NK cells directly to become cytotoxic for targets bearing HIV-1 antigenic determinants. This non-MHC-restricted cytotoxic activity is present in early stages of disease an
J Med Chem. 1990 May;33(5):1400-6. Unique Identifier : AIDSLINE MED/90230244
Farrow SN; Jones AS; Kumar A; Walker RT; Balzarini J; De Clercq E; Chemistry Department, University of Birmingham, Great Britain.
A series of aryl bis(3 -O-acetylthymidin-5 -yl) phosphate derivatives have been synthesized in order to find a suitable aryl derivative which would hydrolyze to the bis(nucleosid-5 -yl) phosphate under physiological conditions. The 4-(methylsulfonyl)phenyl derivative was selected and 4-(methylsulfonyl)phenyl bis[(E)-5
J Med Chem. 1990 May;33(5):1470-6. Unique Identifier : AIDSLINE MED/90230254
Cavalleri B; Turconi M; Tamborini G; Occelli E; Cietto G; Pallanza R; Scotti R; Berti M; Romano G; Parenti F; Merrell Dow Research Institute--Lepetit Research Center,; Gerenzano, Italy.
A series of derivatives of rifamycin P, an antibiotic produced by fermentation of a mutant strain of Nocardia mediterranea or by chemical modification of rifamycin S, have been prepared. The structures of these compounds were determined by 1H NMR, IR, UV, and LC/MS. Their in vitro and in vivo antibacterial activities
J Med Chem. 1990 May;33(5):1505-10. Unique Identifier : AIDSLINE MED/90230259
Aggarwal SK; Gogu SR; Rangan SR; Agrawal KC; Department of Pharmacology, Tulane University School of Medicine,; New Orleans, Louisiana 70112.
A series of prodrugs of zidovudine ( AZT ) has been synthesized in an effort to enhance the uptake of the prodrugs by the HIV-1 infected cells and to increase the plasma half-life of AZT. The 5 -OH function of AZT was esterified with various acids in th
Endo Y; Mamiya S; Iwamoto K; Niitsu H; Itoh T; Miura A; 3rd Department of Internal Medicine, Akita University.
Among 300 patients receiving blood and/or blood products because of blood disorders, 3 (1%) were positive for HIV by ELISA. However, 3 patients were thought to be false positive because they had received bolus injection of gamma-globulin and their serum became negative after 6 months. Moreover, no viral inclusion or H
We studied the prevalence of liver disease and the carrier state for hepatitis B (HBV), delta virus (HDV) and HIV-1 infection in two Valencian penitentiaries, one for males and the other for females. Serological results were correlated with history of intravenous drug addiction, alcohol abuse, homosexuality or prostit
AIDS is one of the most pressing challenges facing health care professionals today. Nurse educators are determining the role of the student in this new clinical situation. This case study provides a look at some of the core issues that may be encountered when a nursing student cares for a patient with this deadly dise
We determined intrathecal synthesis (ITS) of anti-HIV-1 immunoglobulin in 62 CSF samples from 51 HIV-1 seropositive homosexual men using an ELISA technique with paired serum and CSF samples diluted to a concentration of IgG of 10 micrograms/ml. All subjects were neurologically normal and none was taking zidovudine. We
Many people with Aids develop a range of neurological manifestations, which can cause great distress to them and their loved ones. Nurses are ideally placed to offer both practical and psychological support--if it is required.
Wolcott DL; Sullivan G; Klein D; Department of Psychiatry and Biobehavioral Sciences, University; of California, Los Angeles, School of Medicine.
Gay male physicians were surveyed in 1984 and 1985 about their knowledge and attitudes regarding HIV transmission and AIDS and changes they had made in social, health-related, and sexual activities since the onset of the AIDS epidemic. Most of the 37 subjects who participated in both surveys progressively lessened the
Santos I; Garcia-Polo I; del Arco C; Vivancos J; Quijano Y; Padilla B; Herraiz C; Ortega O; Noguerado A; Servicio de Medicina Interna-Infecciosas, Hospital de la; Princesa, Madrid.
Spinal fluid from 53 HIV infected patients has been reviewed. A diagnosis was made exclusively from the spinal fluid exam in 15 cases while in 15 others it contributed to diagnosis together with other exams. The exam did not contribute any positive data in 23 cases. AIDS diagnosis was obtained in 11 patients by the sp
del Rio C; Soffer O; Widell JL; Judd RL; Slade BA; Department of Medicine, Emory University School of Medicine,; Atlanta, Georgia.
A previously healthy 29-year-old homosexual man presented with a 4-day history of fever, malaise, sore throat, and bleeding gums. Rhabdomyolysis, acute renal failure, and nephrotic range proteinuria were also present. The patient was found to have acute human immunodeficiency virus (HIV) infection confirmed by the pre
Robinson PA; Knirsch AK; Joseph JA; Pfizer Central Research, Groton, Connecticut 06340.
Fluconazole therapy was evaluated prospectively in patients with serious fungal infections who failed to respond to or could not tolerate conventional antifungal therapy. Patients were enrolled if they had a life-threatening fungal infection and conventional therapy had failed to eradicate the infection, had caused se
Salud Publica Mex. 1990 Jan-Feb;32(1):20-5. Unique Identifier : AIDSLINE MED/90232374
Gongora-Biachi RA; Arcila-Herrera H; Gonzalez-Martinez P; Franco-Monsreal J; Puerto-Manzano F; Martinez-Reynoso A; Kuyoc-Castro E; Centro de Investigaciones Regionales Dr. Hideyo Noguchi,; Universidad Autonoma de Yucatan, Merida.
Between August/1987, and February/1988, 60 homosexual-men from Cancun, Q. Roo, Mexico , were studied to determine the prevalence of HIV antibodies (Elisa and Western Blot). Six subjects were seropositive. No relationship was found between HIV antibodies and age, socioeconomic level
Salud Publica Mex. 1990 Jan-Feb;32(1):26-37. Unique Identifier : AIDSLINE MED/90232375
Gonzalez Block MA; Liguori AL; Bazua LF; Colegio de la Frontera Norte, Tijuana, Baja California.
The prevalence of AIDS in Mexico and the frequency of sexually transmitted risks was analyzed in relation to the socioeconomic strata of the patients. The study was based on the obligatory notifications of AIDS received by the Ministry of Health between 1982 and september of 1988.
Salud Publica Mex. 1990 Jan-Feb;32(1):3-14. Unique Identifier : AIDSLINE MED/90232376
Perez-Padilla JR; Ponce de Leon-Rosales S; Departamento de Fisiologia Pulmonar del Instituto Nacional de; Enfermedades Respiratorias.
We evaluated, with a questionnaire, ethical attitudes towards the clinical attention of patients with AIDS in 88 physicians. Most of the surveyed were residents and all were working in two mexican hospitals with experience managing patients with AIDS: The National Institute of Nutrition and the National Institute of R
Salud Publica Mex. 1990 Jan-Feb;32(1):38-42. Unique Identifier : AIDSLINE MED/90232377
Vazquez-Valls E; Torres-Mendoza BM; Jauregui-Rios ML; Campos-Lopez PI; Instituto de Patologia Infecciosa y Experimental Dr. Francisco; Ruiz Sanchez de la Universidad de Guadalajara.
In Mexico , since may 1986, the mandatory detection of antibodies against HIV in blood and derivates for the use by humans was legislated, one year later the prohibition of blood trade took effect. The impact of the Sanitary Normativity in the seroprevalence of anti HIV antibodies b
Salud Publica Mex. 1990 Jan-Feb;32(1):80-3. Unique Identifier : AIDSLINE MED/90232382
Fineberg HV; Escuela de Salud Publica, Universidad de Harvard.
Since the AIDS epidemic was first detected, it has had a profound influence on the organization of health services in the United States . In this essay, some of the more relevant aspects of this process are analyzed, and the main problems that need to be confronted in order to fight suc
Salud Publica Mex. 1990 Jan-Feb;32(1):84-7. Unique Identifier : AIDSLINE MED/90232383
Gorab-Ramirez A; Hernandez-Gamboa LE; Rescala Baca E; del Rio Gonzalez B; Niesvizky Iszaevich R; Direccion General de Servicios Medicos UNAM.
The prevention of HIV infection in school and university communities acquires great importance in adolescents and young adults where the risk of HIV infection is caused by age, occupation, location, and also adjoining relations with other groups of high incidence of infection, such as teachers and administrative emplo
Seiki M; Hikikoshi A; Yoshida M; Department of Viral Oncology, Cancer Institute, Tokyo, Japan.
The long terminal repeat (LTR) of HTLV-1 consists of U3-R-U5 and contains elements which regulate the viral gene expression. In addition to these elements, we found that the U5 sequence contained a repressive element in the gene expression. Sequential deletions of the LTR substantially increased CAT expression of a LT
Am J Trop Med Hyg. 1990 Apr;42(4):365-73. Unique Identifier : AIDSLINE MED/90233454
Matsumoto M; Sugimoto M; Nakashima H; Imamura F; Kawano O; Uyama E; Takatsu K; Araki S; Institute for Medical Immunology, Kumamoto University Medical; School, Japan.
Patients with human T lymphotropic virus type I (HTLV-I)-associated myelopathy (HAM) show increased serum levels of soluble interleukin-2 receptors (sIL-2R), a marker of T cell activation. We found that peripheral blood lymphocytes from HAM patients proliferated spontaneously and released sIL-2R when cultured in vitro
Ann Intern Med. 1990 May 15;112(10):750-7. Unique Identifier : AIDSLINE MED/90233582
Jules-Elysee KM; Stover DE; Zaman MB; Bernard EM; White DA; Memorial Sloan-Kettering Cancer Center, New York, New York.
STUDY OBJECTIVE: To determine the effect of previous aerosolized pentamidine therapy on diagnosis and presentation of Pneumocystis carinii pneumonia. DESIGN: A retrospective study. SETTING: A tertiary care hospital. PATIENTS: Fifty-two consecutive patients with P. carinii pneumonia and underlying infection with the hu
Dorsett BH; Cronin W; Ioachim HL; Department of Pathology, Lenox Hill Hospital, New York, NY 10021.
The presence of antilymphocyte antibody (ALA) in patients with the acquired immunodeficiency syndrome (AIDS), identified in a previous study, was confirmed by testing a population of 200 patients with AIDS. Of these, 88% had significant levels of ALA vs only 8% of a control group of patients with non-AIDS-related dise
1840 homosexual and bisexual men were tested for human immunodeficiency virus type I (HIV) infection at the Landesinstitut fur Tropenmedizin, Berlin between April 1983 and December 1987. A total of 25.1% were seropositive for HIV, but the percentage was seemingly declining. During the study period we observed also a s
Can J Public Health. 1990 Mar-Apr;81(2):125-8. Unique Identifier : AIDSLINE MED/90235133
Kerr CI; Horrocks MJ; Dalhousie University.
To assess the knowledge, values, attitudes and behavioural intent of Nova Scotia nurses toward AIDS and patients with AIDS, questionnaires were sent to a random sample of 400 general duty nurses. Analysis of the 179 returned questionnaires (45%) showed that although 88% of the nurses indicated that they intended to nu
Can J Public Health. 1990 Mar-Apr;81(2):129-34. Unique Identifier : AIDSLINE MED/90235134
Adrien A; Boivin JF; Tousignant Y; Hankins C; Department of Community Health, Montreal General Hospital,; Canada.
We used interviews of 268 subjects to ascertain knowledge, attitudes, beliefs and practices related to AIDS among Montrealers of Haitian origin, from November 1987 through October 1988. The questions on knowledge included items on general knowledge about the disease (prevention of AIDS, treatment, nature and manifesta
Can J Public Health. 1990 Mar-Apr;81(2):135-8. Unique Identifier : AIDSLINE MED/90235135
Dolan R; Corber S; Zacour R; Affairs Division, Ottawa-Carleton Health Dept., University of; Ottawa.
With parental consent, we surveyed 942 students in grade 7 and 8 at the Ottawa Board of Education before the introduction of compulsory AIDS education. Television was seen as the most common and credible source of information about AIDS. Parents (by girls) and school nurse (by boys) were also seen as credible sources
Can J Public Health. 1990 Mar-Apr;81(2):141-5. Unique Identifier : AIDSLINE MED/90235137
Herold ES; Fisher WA; Smith EA; Yarber WA; Department of Family Studies, University of Guelph, Ontario,; Canada.
The prevention of unwanted pregnancy and STD/AIDS are two major goals of health educators. Usually these are discussed as separate goals. In this paper both of these health concerns are presented from a behavioural preventive perspective. Our objective is to outline the essential components to be used by health educat
Zack JA; Arrigo SJ; Weitsman SR; Go AS; Haislip A; Chen IS; Department of Microbiology and Immunology, UCLA School of; Medicine.
Productive infection of human T lymphocytes by HIV-1 is dependent upon proliferation of the infected cell. Nonproliferating quiescent T cells can be infected by HIV-1 and harbor the virus in an inactive state until subsequent mitogenic stimulation. We use a modification of the polymerase chain reaction method, which i
Johnson JE; Anders GT; Hawkes CE; LaHatte LJ; Blanton HM; Pulmonary Disease Service and Infectious Disease Service, Brooke; Army Medical Center, Fort Sam Houston, Tex.
To evaluate bronchoalveolar lavage (BAL) findings in patients infected with human immunodeficiency virus (HIV), 39 patients seropositive for the virus but with no history of opportunistic infection were studied. Opportunistic organisms such as Pneumocystis carinii were not found in any of the 35 BAL fluids sent for sp
Reiss TF; Golden J; Department of Medicine, University of California, San Francisco.
Pneumocystis carinii pneumonia was suggested by a diffuse, bilateral pulmonary uptake of gallium-67 in an asymptomatic, homosexual male with the antibody to the immunodeficiency virus (HIV) who was undergoing staging evaluation for lymphoma clinically localized to a left inguinal lymph node. Chest radiograph and pulmo
Health Bull (Edinb). 1990 Mar;48(2):62-72. Unique Identifier : AIDSLINE MED/90236697
Chalmers JW; Department of Community Medicine, Lothian Health Board,; Edinburgh.
The intravenous drug abusers of Edinburgh are known to have a high prevalence of infection with the Human Immunodeficiency Virus (HIV). A new service for the treatment of drug abusers in Edinburgh was commenced in April 1988. In this service, oral methadone substitution is frequently used to try to reduce the level of
Dimitrov DH; Melnick JL; Hollinger FB; Division of Molecular Virology, Baylor College of Medicine,; Houston, Texas 77030.
To define the optimal conditions for human immunodeficiency virus (HIV) detection in microcultures, experiments were conducted with different ratios of patient and donor peripheral blood mononuclear cells (PBMCs). Donor/patient PBMC ratios ranged from 1:1 to 1:125. Optimal results were obtained when 1,500,000 donor ce
Beebe JL; Briggs LC; Laboratory Division, Colorado Department of Health, Denver 80220.
Five commercial enzyme-linked immunoassay systems for the detection of human immunodeficiency virus type 1 antibody from filter paper disks impregnated with whole blood were evaluated for technical and operational performance. All five systems performed adequately in the technical challenges posed, with specificities
Starkey CA; Yen-Lieberman B; Proffitt MR; Department of Microbiology, Cleveland Clinic Foundation, Ohio; 44195.
The Recombigen HIV-1 Latex Agglutination (LA) Test was recently licensed by the U.S. Food and Drug Administration for use as a rapid screening assay for human immunodeficiency virus type 1 (HIV-1) antibodies. However, its performance in various settings and in different populations has not been firmly established. Con
Acute exanthema occurs in patients who are human immunodeficiency virus (HIV) positive before they become seropositive. The patients have influenza like symptoms and a macular skin rash on the upper trunk. Histopathological investigation of skin punch biopsy specimens from four patients with acute HIV exanthema showed
Goldin RD; Fish DE; Hay A; Waters JA; McGarvey MJ; Main J; Thomas HC; Department of Histopathology, St Mary's Hospital Medical School,; London.
Because the risk factors for human immunodeficiency virus (HIV) infection and hepatitis B (HBV) are similar and therefore coinfection is not uncommon, a detailed histological and immunohistochemical study of chronic hepatitis B infection in a group of 20 HIV positive Caucasian males (who did not have AIDS) and 30 HIV
Bond S; Rhodes T; Philips P; Setters J; Foy C; Bond J; University of Newcastle upon Tyne.
A postal survey of 5243 community nursing staff throughout England sought information about their experience, education, knowledge, confidence and beliefs in relation to the human immunodeficiency virus (HIV) and the acquired immune deficiency syndrome (AIDS). A response rate of 74% was obtained. While experience of p
Bartolome FJ; Moraleda G; Castillo I; Martinez MG; Porres JC; Carreno V; Department of Gastroenterology, Fundacion Jimenez Diaz,; Universidad Autonoma, Madrid, Spain.
The presence of hepatitis B virus DNA (HBV-DNA) in the peripheral blood mononuclear cells (PBMC) of 29 anti-HIV symptomless carriers (eleven HBeAg positive, eleven anti-HBe positive and seven HBsAg negative) and of 40 anti-human immunodeficiency virus (HIV)-negative patients (15 HBeAg positive, 15 anti-HBe positive an
Zygmunt DJ; Department of Medicine, Vanderbilt University School of Medicine,; Nashville, Tennessee 37232-2561.
Toxoplasmosis encompasses a variety of clinical conditions. Serious sequelae are seen in congenital toxoplasmosis and in infections of the immunocompromised host. In the former, prevention of maternal acquisition of toxoplasmosis during pregnancy is paramount. Infection in the compromised host often presents with neur
Saito S; Ando Y; Furuki K; Tanigawa T; Kakimoto K; Moriyama I; Ichijo M; Department of Obstetrics and Gynecology, Nara Medical University.
We developed a new method to detect human T cell leukemia virus type I (HTLV-I) provirus by amplifying the pX region of the HTLV-I genome by means of the polymerase chain reaction (PCR) method. This new method was used to examine babies delivered by HTLV-I carrier women for HTLV-I vertical transmission. It was found t
J Math Biol. 1990;28(3):271-91. Unique Identifier : AIDSLINE MED/90237689
Artzrouni M; Department of Mathematical Sciences, Loyola University, New; Orleans, LA 70118.
During the initially exponential spread of the human immunodeficiency virus (HIV--the causative agent of AIDS) the growth rate of the number of AIDS cases decreased from plus infinity to the growth rate of HIV infections. A sensitivity analysis shows that for all reasonable values of the parameters of the HIV epidemic
Mikovits JA; Raziuddin; Gonda M; Ruta M; Lohrey NC; Kung HF; Ruscetti FW; Department of Biological Carcinogenesis Development Program,; Program Resources, Inc., Frederick, Maryland.
In THP-1 monocytoid cells infected with HIV, viral expression can be regulated in several ways: (a) latency (no viral expression); (b) restricted expression (chronic low-level viral expression with little or no detectable virus released); and (c) continuous production. In cells with restricted HIV expression, nuclear
Nickens H; Division of Minority Health, Association of American Medical; Colleges, Washington, DC 20036.
In the black community, many health indicators are going in the wrong direction. Black life expectancy is going down. Our middle class is growing but our poor are growing also. It is no longer even jarring to talk about black males as an endangered species. Black women are groaning under family responsibilities. Too m
Eskander GS; Jahan MS; Carter RA; Meharry Medical College, Nashville, Tennessee.
To study the ethnic differences in knowledge, attitudes, and beliefs about AIDS, we surveyed 161 US residents from 31 different countries. An anonymous self-administered questionnaire was developed to compile these data. The results of this survey suggest that AIDS-related knowledge is generally incomplete and there a
Proc Natl Acad Sci U S A. 1990 May;87(9):3445-9. Unique Identifier : AIDSLINE MED/90239030
Devash Y; Calvelli TA; Wood DG; Reagan KJ; Rubinstein A; E. I, du Pont de Nemours, Medical Products Department, Glasgow.
Many, but not all, infants born to mothers infected with the human immunodeficiency virus (HIV) are infected in utero. We have now shown that mothers who have high-affinity/avidity antibodies directed toward the principal neutralizing domain (PND) of gp120 are less likely to transmit HIV to their children. An ELISA th
Proc Natl Acad Sci U S A. 1990 May;87(9):3534-7. Unique Identifier : AIDSLINE MED/90239049
Camerman N; Mastropaolo D; Camerman A; Department of Biochemistry, University of Toronto, ON, Canada.
The crystal and molecular structures of the anti-human immunodeficiency virus agent 3 -fluoro-3 -deoxythymidine have been determined by x-ray diffraction and stereochemical comparisons with thymidine have been made. Atomic charge distributions have been calculated by the complete neglect of differential overlap method
Proc Natl Acad Sci U S A. 1990 May;87(9):3624-8. Unique Identifier : AIDSLINE MED/90239066
Marciniak RA; Garcia-Blanco MA; Sharp PA; Center for Cancer Research, Massachusetts Institute of; Technology, Cambridge.
Human immunodeficiency virus type 1 RNAs contain a sequence, trans-activation-response (TAR) element, which is required for tat protein-mediated trans-activation of viral gene expression. We have identified a nuclear protein from extracts of HeLa cells that binds to the TAR element RNA in a sequence-specific manner. T
Singapore Med J. 1990 Feb;31(1):33-7. Unique Identifier : AIDSLINE MED/90239616
Ratnam KV; Middle Road Hospital, Singapore.
A study of the awareness of Acquired Immunodeficiency Syndrome (AIDS) among Transsexual prostitutes attending the Middle Road Hospital was made. The present study involved 71 subjects of a cohort of 100 who were interviewed a year earlier and had subsequently been given intensive health education. The results show tha
Ter Arkh. 1990;62(1):97-9. Unique Identifier : AIDSLINE MED/90239781
Kalikanov SA; Chabanenko AI; Reznikov IuP; Arakelov SA; Stakhanova VM
The authors describe a patient who demonstrated positive blood responsiveness to the nuclear antigens of human immunodeficiency virus (HIV) (p17, p31 and p55), observed steadily for 1 year and 4 months. The donor s disease history consideration made it impossible to include him in one of the known groups at risk for H
Mangos JA; Doran T; Aranda Naranjo B; Rodriguez-Escobar Y; Scott A; Setzer JR; South Texas Children's AIDS Center, Department of Pediatrics,; University of Texas Health Science Center, San Antonio.
For 9 years we have recognized HIV infection/AIDS as an unstoppable epidemic affecting infants, children, and adolescents as well as adults. Now we see the tremendous impact of this disease on the health systems of this country. During these past 9 years, we have witnessed the transformation of the epidemic from one p
Goldschmidt RH; Family Practice Inpatient Service, San Francisco General; Hospital, CA 94110.
The health care worker s exposure to HIV presents a cascade of real and potential problems. Medical, psychological, employment, and legal problems present ongoing challenges, often in uncharted territory. The references listed below provide additional information to help in both individual cases and in prospectively s
Roscoe B; Kruger TL; Central Michigan University, Mount Pleasant 48859.
Approximately 300 late adolescents were surveyed to assess their knowledge of AIDS, and to determine if and how their sexual behavior had changed as a result. Findings suggested that late adolescents were quite knowledgeable regarding AIDS and its transmission; however, only about one-third had altered their sexual be
Armstrong MY; Hrdy DB; Carlson JR; Friedlaender JS; Department of Epidemiology, Yale University School of Medicine,; New Haven, Connecticut 06510.
Serum samples obtained in 1986 from healthy individuals in three distinct Solomon Islands populations were screened for antibodies to human lymphotropic virus type I (HTLV-I). One of the populations tested lives on the remote Polyne
Aebischer ML; Martorana MC; Costa F; Battaggia C; Madera A; Destito D; Machera F; Bailly C; Angeloni P; National Center of Blood Transfusion, Italian Red Cross, Rome,; Italy.
Microfilter absorbed whole blood samples from 223 Tanzanian babies and 189 adults from Cameroon have been examined. Blood specimens are difficult to obtain from African suburban and rural areas, and lack of storage and transportation facilities can prevent the collection of sampl
Morse GD; Portmore A; Olson J; Taylor C; Plank C; Reichman RC; Department of Pharmacy, State University of New York, Buffalo.
The disposition of zidovudine (ZDV) was examined during chronic oral dosing (300 mg every 4 h while awake) for 12 weeks in eight asymptomatic patients with hemophilia who were infected with the human immunodeficiency virus. Pharmacokinetic studies were conducted at the initiation of drug administration and after 6 and
Larder BA; Chesebro B; Richman DD; Department of Molecular Sciences, Wellcome Research Laboratories,; Beckenham, Kent, United Kingdom.
Conventional assays based on infection of T-cell lymphoblastoid lines with tissue culture-adapted strains of human immunodeficiency virus (HIV) are well established and have been used successfully to discover potent inhibitors of HIV replication. In this report we show that such assays are not easily applied to testin
Antoniskis D; Larsen RA; Los Angeles County-University of Southern California Medical; Center 90033.
We report four cases of acute reversible renal failure in patients with acquired immune deficiency syndrome who received both amphotericin B (for systemic mycoses) and pentamidine isethionate (for Pneumocystis carinii pneumonia). The concurrent use of amphotericin B with either inhaled pentamidine or trimethoprim-sulf
Freed CR; Breeze RE; Rosenberg NL; Schneck SA; Wells TH; Barrett JN; Grafton ST; Huang SC; Eidelberg D; Rottenberg DA; Department of Medicine, University of Colorado Health Sciences; Center, Denver 80262.
In an effort to improve the clinical signs of Parkinson s disease, we have implanted mesencephalic dopamine cells from a 7-week human embryo into the caudate and putamen of a 52-year-old man with Parkinson s disease. Fetal tissue was obtained from elective abortion. The woman and the patient with Parkinson s disease w
Benghuzzi HA; Barbaro RM; Bajpai PK; University of Dayton, Ohio 45469.
Conventional methods of administration of 3-azido-2,3-dideoxythymidine ( AZT ) to treat AIDS patients is associated with negative side effects, including toxic effects on bone marrow. This study was designed to investigate the capability of tricalcium p
Stimson GV; Centre for Research on Drugs and Health Behaviour, Charing Cross; and Westminster Medical School, London, UK.
British drug policies are undergoing a major reassessment and reformulation in response to problems raised by HIV disease and AIDS. The years from 1986 to 1989 are one of the key periods of crisis and transformation in the history of the British response to drug problems, with the emergence in current debate of a new
Klee H; Faugier J; Hayes C; Boulton T; Morris J; Centre for Health Education Studies, Manchester Polytechnic, UK.
The sexual mediation of HIV infection by injecting drug users to the heterosexual population has become a major issue in AIDS prevention. Infection that is acquired through sharing contaminated injecting equipment can be passed on to non-injecting drug using partners and to non-drug using partners through sexual inter
Krcmery V; Catar G; Hlavkova Z; Kollarova E; Sobota K; Bohunicka L; Katedry klinickej onkologie ILF v Bratislave.
The authors analyze ethico-philosophical and socio-legal problems of patients with AIDS in their relation to people they are in frequent contact with, i.e. family, relatives, sexual partners, as well as medical and paramedical staff. They deal with the problem of compulsory notification of the disease on the one hand,
Obata S; Tsukamoto A; Kimura K; Maeda K; Kawamura R; Department of Gastroenterology, Kumamoto National Hospital,; Japan.
A patient with gastric involvement of adult T-cell leukemia is reported. Endoscopically, it mimicked an early gastric cancer IIa + IIc, but had the nature of a submucosal tumor, and could be distinguished from carcinoma. It is very important to distinguish it from carcinoma because of the different mode of therapy.
Gen Hosp Psychiatry. 1990 May;12(3):166-9. Unique Identifier : AIDSLINE MED/90243139
Blumenfield M; Milazzo J; Wormser GP; Department of Psychiatry, New York Medical College, Valhalla.
Compliance with treatment is an important concern in the management of patients with a wide array of medical disorders. A chart review of 20 hospitalized AIDS patients at one medical center was done to determine the degree of noncompliance in this extremely ill population. Ninety percent of AIDS patients declined some
Plebani A; Biolchini A; Bucceri A; Buscaglia M; Pardi G; Semprini AE; First Department of Pediatrics, University of Milan, Medical; School, Italy.
Human immunodeficiency virus (HIV) has been isolated from fetal tissues as early as 13 weeks and later from fetal blood. These findings have raised the possibility of prenatal diagnosis of infected fetuses by identification of the virus in the fetal compartment. Study of the fetal immune status has proved reliable in
Valle A; Garrone P; Yssel H; Bonnefoy JY; Freedman AS; Freeman G; Nadler LM; Banchereau J; UNICET, Laboratory for Immunological Research, Dardilly, France.
A new monoclonal antibody (mAb) of the IgG1 subclass, mAb 104, has been obtained after immunization of mice with the Burkitt lymphoma cell line Jijoye. It only weakly binds to a small proportion of non-activated normal B cells and binds to a larger proportion of in vitro-activated normal B cells. All tested Epstein-Ba
Nyborg JK; Dynan WS; Department of Chemistry and Biochemistry, University of Colorado,; Boulder 80309.
Nuclear extracts of cultured human cells contain multiple proteins that recognize specific sequence elements within the transcriptional control region of the human retrovirus, human T-cell leukemia virus type I (HTLV-I). Here we report the purification of host expression factor 1T (HEF-1T), a DNA-binding protein from
Clark R; Greer DL; Carlisle T; Carroll B; Section of Infectious Disease, Tulane University, New Orleans,; LA.
Zygomycosis, an invasive fungal infection, is usually seen in persons with diabetes, particularly in those with diabetic ketoacidosis. The infection most frequently occurs in the rhinocerebral region and rapidly spreads, causing a swift demise. Rarely, the infection is confined to the cutaneous tissues. We describe a
Carvalho P; Bannerjee AK; Goodman A; Department of Radiology, Westminister Hospital, London, U.K.
We present two human immunodeficiency virus positive patients with rectal lymphoma who on CT both demonstrated similar appearances of the rectal tumour, consisting of concentric thickening of the rectal wall and thickening of the levator ani muscles.
Sugimoto M; Araki S; First Department of Internal Medicine, Kumamoto University; Medical School, Japan.
Patients with HTLV-I-associated myelopathy (HAM) show subclinical pulmonary involvement. Bronchoalveolar lavage (BAL) studies of HAM patients have shown that there is T-lymphocytosis in association with increased soluble IL-2 receptor levels in BAL fluid. Furthermore,
Schwartz S; Felber BK; Benko DM; Fenyo EM; Pavlakis GN; Department of Virology, School of Medicine, Karolinska Institute,; Stockholm, Sweden.
We have used the polymerase chain reaction technique to clone the small multiply spliced mRNA species produced after infection of human cells by a molecular clone of human immunodeficiency virus type 1 (HIV-1). We identified six Rev-expressing mRNAs, which were generated by the use of two splice acceptors located imme
Ellison V; Abrams H; Roe T; Lifson J; Brown P; Department of Pediatrics, Stanford University Medical Center,; California 94305-5428.
Integration of the viral genome into the nuclear DNA of a host cell plays a pivotal role in the replication of retroviruses. We have developed an in vitro method for studying the biochemistry of human immunodeficiency virus (HIV) integration by using extracts from HIV-infected cells. Analysis of the reaction products
Greaves RF; O'Hare P; Marie Curie Research Institute, Oxted, Surrey, United Kingdom.
Herpes simplex virus type 1 virion protein Vmw65 forms a complex (TRF.C) with TAATGARAT sequences and the cellular transcription factor oct-1, which has been implicated as an intermediate in the activation of gene expression by Vmw65. To examine structural requirements within Vmw65 for this interaction, we analyzed ex
Toyoshima H; Itoh M; Inoue J; Seiki M; Takaku F; Yoshida M; Department of Viral Oncology, Cancer Institute, Tokyo, Japan.
Rex protein of human T-cell leukemia virus type 1 (HTLV-1) induces cytoplasmic expression of unspliced gag/pol mRNA and singly spliced env mRNA and thus is essential for replication of the virus. This regulation requires a cis-acting rex-responsive element (RXE), located in the 3 region of the viral RNA. By external
Fenouillet E; Gluckman JC; Bahraoui E; CERVI, Groupe Hospitalier Pitie-Salpetriere, Paris, France.
We have shown that enzymatic removal of N-linked glycans from human immunodeficiency virus type 1 (HIV-1) recombinant envelope glycoproteins gp160 and gp120 produced in BHK-21 cells did not significantly reduce their ability to bind to CD4, the cellular receptor for the virus. Because recombinant proteins may behave d
Molina JM; Scadden DT; Amirault C; Woon A; Vannier E; Dinarello CA; Groopman JE; Division of Hematology/Oncology, New England Deaconess Hospital,; Harvard Medical School, Boston, Massachusetts.
The production of interleukin-1 beta (IL-1 beta), IL-6, and tumor necrosis factor alpha (TNF-alpha) by fresh peripheral blood mononuclear cells was evaluated after exposure to human immunodeficiency virus (HIV) or purified recombinant HIV-1 envelope glycoprotein (rgp120). To exclude the role of contaminating endotoxin
Levrero M; Balsano C; Natoli G; Avantaggiati ML; Elfassi E; I Clinica Medica, Universita di Roma La Sapienza, Italy.
The X gene product of the hepatitis B virus (HBV) has been expressed transiently in HepG2 cells, and the 17-kilodalton protein has been detected by Western (immuno-) blot analysis. Cotransfection of the X gene with the long terminal repeat of human immunodeficiency virus type 1 or 2 results in a stimulation of long te
Renjifo B; Speck NA; Winandy S; Hopkins N; Li Y; Center for Cancer Research, Massachusetts Institute of; Technology, Cambridge 02139.
A series of 5 deletions and a point mutation in the binding site for nuclear factor kappa B were introduced into the U3 region of a molecular clone of simian immunodeficiency virus from macaques (SIVmac142). The transcriptional activity of the mutated U3 regions was analyzed by transient chloramphenicol acetyltransfe
Bray LF; Kansas Department of Health and Environment, Topeka.
Clearly, medical professionals and educators must mobilize in supporting personal, professional and public AIDS education efforts. AIDS health education should continue to emphasize facts and dispel myths about risk associated with blood donation, casual contact, mosquitos and sharing eating utensils. Messages should
Med Clin (Barc). 1990 Jan 13;94(1):5-8. Unique Identifier : AIDSLINE MED/90244772
Soriano V; Tor J; Ribera A; Clotet B; Muga R; Pauplana M; Abella E; Fernandez JL; Flores A; Foz M; Unidad de Enfermedades Infecciosas (Servicio de Medicina; Interna), Hospital de Badalona Germans Trias i Pujol, Barcelona.
HTLV-1 was the first human retrovirus to be isolated. It has been shown that it is the causative agent of T cell leukemia in the adult and some types of subacute myelopathies. The virus is transmitted by similar routes as the AIDS virus. HTLV-1 infection is endemic in South Japan , t
Mangos JA; Doran T; Aranda-Naranjo B; Rodriguez-Escobar Y; Scott A; Setzer JR; South Texas Children's AIDS Center, Department of Pediatrics,; University of Texas Health Science Center, San Antonio; 78284-7802.
In order to provide comprehensive care to infants, children, and adolescents with HIV infection/AIDS, a systematic approach to clinical management had to be developed. This approach involves case management by experienced health professionals, including initial evaluation and needs assessment, planning of services, br
Medaglini S; Comi G; Galardi G; Locatelli T; Comola M; Nemini R; Lazzarini A; Castagna A; Canal N; IV Neurologic Department, University of Milan, S. Raffaele; Hospital.
About 30-40% of AIDS patients present CNS and/or PNS involvement, due to direct action of HIV virus or opportunistic infections. Nervous system involvement in the HIV correlated syndromes is not a rare occurrence; nevertheless no studies about prognosis of AIDS related syndromes have been published yet. We tested 38 H
Vago L; Trabattoni G; Lechi A; Cristina S; Budka H; V Cattedra di Anatomia ed Istologia Patologica dell'Universita di; Milano.
109 brains were selected after the exclusion of those affected by space occupying lesions or opportunistic infections. All brains with HIV specificity (nodules with multinucleated cells and, at times, two peculiar kinds of myelin involvement) were atrophic (62%); nevertheless dementia
Bono G; Zandrini C; Brusta R; Sinforiani E; Barbarini G; Moglia A; Neuro-Aids Unit, IRCCS C. Mondino, Universita di Pavia, Italy.
A group of n. 86 HIV-pts. (CDC stages from II to IV) were cross-sectionally studied with neuropsychological and neurophysiological methods. The percentage of abnormalities detected even among pre-AIDS stages is very high (from 25 to 50% of the cases). Sensibility and specificity of the above investigations is discusse
Scalzini A; Scura G; Stellini R; Cristini G; 1st Division of Infectious Diseases, Spedali Civili, Brescia.
HIV infection may display neurological symptoms at any stage; the virus can be isolated from the cerebrospinal fluid (CSF) of both symptomatic and asymptomatic patients and of two third of patients with AIDS. This study sought to determine the sensitivity of HIV1-Ag in the CSF of an HIV-Ab positive population to evalu
Catania S; Nobili C; Trinchieri V; Mascellino MT; Cirelli A; Institute of Infectious Diseases, La Sapienza, University of; Rome, Italy.
A black heterosexually HIV-infected woman, initially presented with cryptococcal meningitis (satisfactorily responding to fluconazole treatment), which was soon followed by lethal cerebral toxoplasmosis.
Grippo A; Ghidini P; Mazzotta F; di Pietro M; Bartolozzi D; Milo D; Mazzotta D; Paci P; Pinto F; Department of Neurological and Psychiatric Sciences, University; of Florence.
Spinal and cortical SEP responses were recorded during tibial and median nerve stimulation in 58 HIV+ subjects (8 IV/C1, 24 IV/C2 & IV/A, 11 III and 15 II), all asymptomatic from a neurological point of view. The electrophysiological features were compared with clinical assessment and serum HIV markers for purposes
Ferrante P; Seresella M; Cocuzza CE; Caputo D; Cattaneo E; Achilli G; Cazzullo CL; Multiple Sclerosis Center, Milan, Italy.
The sera and the CSFs from 157 Multiple Sclerosis (MS) patients and 43 other neurological diseases (OND) cases have been evaluated for the presence of HTLVI antibodies. A commercial passive agglutination assay (PPA), an indirect fluorescence assay (IFA), and a Western Immunoblotting (WIB) performed in our laboratories
Blood. 1990 May 15;75(10):1920-3. Unique Identifier : AIDSLINE MED/90248558
Zucker-Franklin D; Seremetis S; Zheng ZY; Department of Medicine, New York University Medical Center, NY.
Direct infection of megakaryocytes and platelets by human immunodeficiency virus type I (HIV-I) or other retroviruses has not been demonstrated. To determine whether this could occur, murine bone marrow was co-cultivated with the amphotropic retrovirus-producing cell line PA317-N2, and freshly isolated normal human bo
Inouye Y; Take Y; Tokutake Y; Yoshida T; Yamamoto A; Yamase T; Nakamura S; School of Medicine Hiroshima University, Japan.
A Keggin polyoxotungstate PM-19 K7[PTi2W10O40].6H2O was found to be a potent inhibitor of the replication of human immunodeficiency virus (HIV), which causes acquired immunodeficiency syndrome (AIDS), in OKT4+ cells. In contrast, the effect of HPA 23 (NH4)17Na[NaSb9W21O86], an inhibitor of reverse transcriptase of HIV
Stanley MW; Horwitz CA; Burton LG; Weisser JA; Department of Pathology, Metropolitan-Mount Sinai Medical Center,; Minneapolis, MN.
In patients with the acquired immunodeficiency syndrome (AIDS), four cases of mycobacterial infection in which bacilli appeared as cylindrical, nonstaining negative images have been previously described. These may have been extracellular or within histiocyte cytoplasm, and they have been described in aspirations from
Ozbek MR; Kutlu N; Ankara University Faculty of Medicine, Department of Plastic and; Reconstructive Surgery, Cebeci-Ankara, Turkey.
A case is reported of Kaposi s sarcoma in a very rare location, the palmar surface of the hand. Kaposi s sarcoma, which still has an unresolved etiology has recently gained great interest because of its close association with AIDS. In our case there had been recurrences even after two surgical interventions. The resul
Kent EF Jr; Crawford J; Cohen HJ; Buckley RH; Department of Pediatrics, Duke University Medical Center, Durham,; North Carolina 27710.
We have identified five patients with severe combined immunodeficiency (SCID) who developed multiple monoclonal serum immunoglobulin components (multiclonal gammopathy) following bone marrow transplantation. Four patients received haploidentical bone marrow stem cells depleted of T cells and other mature marrow cells
Takamizawa S; Kaito K; Hori S; Kikuchi A; Hashimoto T; Shimada J; Miyahara T; Jyo K; Ushigome S; Nikaido T; et al; Second Department of Internal Medicine, Jikei University School; of Medicine, Tokyo Japan.
This is a report on the clinical courses and pathological findings in two gay male patients with acquired immunodeficiency syndrome (AIDS) infected in Japan . Case 1. A 39 year-old Japanese homosexual male was diagnosed as amebic dysentery complicated with liver abscess on admission.
Perdices M; Cooper DA; NHMRC Special Unit in AIDS Epidemiology, University of New South; Wales, Australia.
A group of 34 homosexual men with acquired immune deficiency syndrome (AIDS) spectrum disorders were assessed for cognitive impairment on a range of neuropsychological tests. There were 17 patients with AIDS, and 17 with AIDS-related complex (ARC). Although none of the patients showed signs of the severe dementing syn
Cohen EA; Lu Y; Gottlinger H; Dehni G; Jalinoos Y; Sodroski JG; Haseltine WA; Division of Human Retrovirology, Dana-Farber Cancer Institute,; Boston, Massachusetts 02115.
Sequence analysis of multiple isolates of the human immunodeficiency virus type 1 (HIV-1) reveals the existence of a conserved open reading frame, designated T, that partially overlaps the tat, rev, and vpu coding sequences. Here we show that in vitro translation of RNA derived from this region of the viral genome yie
Ahlgren DJ; Gorny MK; Stein AC; Department of Engineering and Computer Science, Trinity College,; Hartford, Connecticut 06106.
By combining dynamic modeling of human immunodeficiency virus (HIV) transmission with mathematical optimization techniques, we calculate values of epidemiological parameters characterizing the early epidemic (1978-1986) among homosexual and bisexual men in San Francisco. The seroconversion fraction data reported by th
Menendez BS; Drucker E; Vermund SH; Castano RR; Perez-Agosto RR; Parga FJ; Blum S; Department of Health Services, Lehman College CUNY, Bronx, New; York 10468.
Analysis of AIDS mortality data for New York City for 1981-1987 reveals that Puerto Ricans represent the racial/ethnic group most severely affected by this city s AIDS epidemic. Cumulative age-adjusted AIDS mortality rates among Puerto Rico-born males are significantly higher (362 per 100,000) than among blacks (267),
Med Care. 1990 May;28(5):468-72. Unique Identifier : AIDSLINE MED/90250975
Weil PA; Stam LM; American College of Healthcare Executives, Chicago, IL 60614.
Executives are just beginning to adapt to the presence of the epidemic. Two-thirds have been involved in AIDS care and these hospitals appear to be in the forefront in developing structural responses and initiating procedures to deal with the disease. Through the use of rating scale analysis, we were able to show a co
Fridland A; Connelly MC; Ashmun R; Department of Biochemical and Clinical Pharmacology, St. Jude; Children's Research Hospital, Memphis, Tennessee 38101.
The metabolism and cytostatic effects of 3 -azido-3 -deoxythymidine ( AZT ), one of the most effective agents being used in the treatment of acquired immunodeficiency syndrome, were investigated in the CCRF-CEM line of human T lymphoid cells. The concen
Belec L; Bouquety JC; Georges AJ; Siopathis MR; Martin PM; Institut Pasteur de Bangui, Central African Republic.
Reports of rare cases of suspected transmission of the human immunodeficiency virus (HIV) from mother to children by breast milk have been recently published. To study the factors that possibly limit HIV transmission through breast-feeding, milk samples obtained from 15 healthy, seropositive mothers and 4 seronegative
Proc Natl Acad Sci U S A. 1990 May;87(10):3695-9. Unique Identifier : AIDSLINE MED/90251623
Syu WJ; Huang JH; Essex M; Lee TH; Department of Cancer Biology, Harvard University School of Public; Health, Boston, MA 02115.
Human immunodeficiency virus (HIV) vaccines targeted at blocking HIV-CD4 interactions are expected to be less affected by the sequence heterogeneity of HIV than those targeted at variable regions of the envelope outercoat glycoprotein, gp120. All potential CD4 binding sites identified thus far in HIV are localized in
Thomasma DC; McElhinney TK; Loyola University of Chicago Stritch School of Medicine, Maywood; 60153.
The HIV/ARC/AIDS story continues to unfold. It is both the old, sadly familiar experience of plague and disease, of lepers isolated as unclean, of smallpox decimating the American Indians, of a Black Death sweeping medieval Europe, of the 1918 influenza. It is also a new story, one in which medical scientists rather q
Schweiz Rundsch Med Prax. 1990 May 2;79(18):547-50. Unique Identifier : AIDSLINE MED/90251867
Somaini B; Bundesamt fur Gesundheitswesen, Bern.
From the institution of an European statistic for the incidence of Aids onwards Switzerland has had the highest per capita rate. The marked increase of Aids victims in drug-addicts over the past years is also well established. An improved surveillance of HIV spread is mandator
Schweiz Rundsch Med Prax. 1990 May 2;79(18):569-73. Unique Identifier : AIDSLINE MED/90251871
von Eiff M; Roos N; Wilms B; Walger P; Baumgart P; Steimann R; van de Loo J; Abteilung Innere Medizin A, Universitat Munster.
Bronchoalveolar lavage was used in 170 immunocompromised patients for detection of causative agents. Pneumocystis carinii was isolated 18 times from HIV-positive patients and 14 times from patients suffering from immunodeficiencies due to various other diseases. Patients with AIDS were in better clinical condition, ha
Nilsen R; Stromme HK; Laake K; Nasjonalforeningens forskergruppe i geriatri.
We reviewed the routines for providing information on drugs, and for training in the use of drugs and aids to medication in hospital and nursing homes by interviewing 11 ward supervisors. We found that little attention was given to systematic training in the use of drugs, training and aids to medication as part of the
Tidsskr Nor Laegeforen. 1990 May 10;110(12):1490-2. Unique Identifier : AIDSLINE MED/90252180
Kraft P; Traeen B; Rise J; Seksjon for epidemiologi, Universitetet i Bergen.
This paper describes use of contraception at first intercourse among Norwegian adolescents and sheds some light upon whether the HIV-epidemic has influenced the use of contraception. The data stem from a population-based survey of 3,000 17-, 18- and 19-year-olds. The response rate was 60.9%. A total of 1,172 persons h
Tidsskr Nor Laegeforen. 1990 May 10;110(12):1509-12. Unique Identifier : AIDSLINE MED/90252185
Harvei S; Langmark F; Haldorsen T; Kreftregisteret Institutt for epidemiologisk, Oslo.
The article analyzes the incidence of Sarcoma Kaposi in Norway during the period 1957-86 (30 years). A clear increase is found for both sexes, particularly over the last 15 years. Whether this is due to changes in diagnostics, classification etc. can best be decided by histopatholo
Tidsskr Nor Laegeforen. 1990 May 10;110(12):1551-4. Unique Identifier : AIDSLINE MED/90252199
Welle-Strand GK; Akuttinstitusjonen, Oslo.
Akuttinstitusjonen (a detoxification clinic) and Uteseksjonen (a municipal outreach program) are two important institutions for drug abusers in the centre of Oslo. Both institutions offer voluntary HIV-antibody testing. 328 HIV-tests were performed on 214 different persons during a three-year period ending 31 December
Clinical observations and empirical findings with regard to possible negative and positive consequences of the HIV-Antibody-Test are discussed. It was concluded that at the present time we should not recommend the test generally for all people, but rather suggest the way of test-counselling. Results of a pilot study d
Am J Hematol. 1990 Jun;34(2):132-9. Unique Identifier : AIDSLINE MED/90252754
Herbert V; Fong W; Gulle V; Stopler T; Hematology and Nutrition Research Laboratory, Veterans Affairs; Medical Center, Bronx, New York.
In AIDS, as previously found in pernicious anemia (PA), the earliest serum marker of subnormal vitamin B12 (cobalamin) absorption, and therefore of negative B12 balance, is low serum holotranscobalamin II (holo-TC II; B12-TC II) despite normal total serum B12 level, normal serum homocysteine, and normal classic (oral
Biochem J. 1990 May 1;267(3):759-66. Unique Identifier : AIDSLINE MED/90253391
Holschbach C; Schneider J; Geyer H; Biochemisches Institut am Klinikum, Universitat Giessen, Federal; Republic of Germany.
The envelope glycoprotein 130 ( 130 referring to an Mr of 130,000) of simian immunodeficiency virus from sooty mangabey (Cercocebus atys) (SIVSM) was isolated from the cell-free supernatant of the SIVSM-infected human T-cell line H9, metabolically labelled with D-[6-3H]glucosamine. After digestion with Staphylococcus
Coan MH; Dobkin MB; Brockway WJ; Mitra G; Research Division, Cutter Biological of Miles Inc., Berkeley,; California.
Alpha 1-proteinase inhibitor (alpha 1-PI) concentrate is prepared from Cohn fraction IV-1 by precipitation and ion-exchange chromatography. A pasteurisation step (60 degrees, 10 hours) is introduced in this process with the aim at lowering the risk of transmitting infectious agents. Characterisation studies of alpha 1
Kraus DH; Rehm SJ; Orlowski JP; Tubbs RR; Levine HL; Department of Otolaryngology and Communicative Disorders,; Cleveland Clinic Foundation, OH 44195.
Head and neck manifestations of human immunodeficiency virus (HIV) infection are common and include diffuse cervical lymphadenopathy, cutaneous and mucosal Kaposi s sarcoma, mucosal herpes simplex infection, upper aerodigestivetractcandidiasis, and parotidlymphadenopathy and cysts. Recurrent otitis media and chronic s
Noble JT; Stearns NS; Wolff SM; Department of Medicine, Tufts University School of Medicine,; Boston, Massachusetts.
Curriculum guidelines for the education of primary care practitioners (PCPs) about AIDS and AIDS-related disorders have been developed by the National Fund for Medical Education (NFME). The guidelines resulted from a modified Delphi authority opinion survey with two iterations and have been edited by the authors. The
AIDS Res Hum Retroviruses. 1990 Mar;6(3):275-85. Unique Identifier : AIDSLINE MED/90253916
Allan JS; Kanda P; Kennedy RC; Cobb EK; Anthony M; Eichberg JW; Department of Virology and Immunology, Southwest Foundation for; Biomedical Research, San Antonio, TX 78284.
Cercopithecus aethiops (African Green monkey), a nonhuman primate species distributed throughout subsaharan Africa, has been shown to have high seroprevalence rates of antibodies to simian immunodeficiency virus (SIV), and therefore, has been proposed as a natural reservoir for immunodeficiency viruses. Our laboratori
The immunoreactivity of HTLV-III-infected individuals and virus-inoculated chimpanzees with gp120 synthetic peptides of the HTLV-III gp120 envelope principle neutralizing domain (amino acid 301-324 sequences), derived from the HTLV-III isolates 3B, RF, MN, WMJ2, and SC were determined. Sequential bleeds from an infect
AIDS Res Hum Retroviruses. 1990 Mar;6(3):357-70. Unique Identifier : AIDSLINE MED/90253923
Lucas C; Peterson ML; Bennett GL; Frie SW; Berman PW; Chen AB; Department of Medicinal and Analytical Chemistry, Genentech Inc.,; SouthSan Francisco, CA 94080.
A radioimmunoprecipitation (RIP) assay was developed to detect antibodies to the envelope glycoprotein gp120 of the human immunodeficiency virus (HIV-1). The assay, which utilized recombinant gp120 (rgp120), was quantitative, reproducible, and specific for antibodies to rgp120 or antibodies to native gp120 resulting f
AIDS Res Hum Retroviruses. 1990 Mar;6(3):417-21. Unique Identifier : AIDSLINE MED/90253929
Manzari V; Modesti M; De Marchis L; Gradilone A; Cifaldi L; Marchei P; Department of Experimental Medicine and Biochemical Science, II; University of Rome Tor Vergata, Italy.
Lymphadenopathy with no apparent cause had been reported in a group of women participating in a mammary tumor prevention program. A screening for retrovirus infection was organized to detect the virus as possible etiological agents. Data show a high percentage of positivity for HIV-1 among these lymphadenopathy patien
AIDS Res Hum Retroviruses. 1990 Apr;6(4):431-41. Unique Identifier : AIDSLINE MED/90253932
Cox RA; Anders GT; Cappelli PJ; Johnson JE; Blanton HM; Seaworth BJ; Treasure RL; Department of Research Immunology, San Antonio State Chest; Hospital, TX 78223.
Tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 (IL-1) are potent immunomodulatory cytokines which are produced principally by cells of the macrophage-monocyte lineage. We conducted an investigation to assess the secretion of these cytokines by bronchoalveolar macrophages from patients with progressive stage
A rapid procedure for the inactivation of HIV-1-infected cells using psoralen and ultraviolet (UV) light is described. Exposure of HIV-1-infected cells to 5 micrograms/ml psoralen followed by UV irradiation (320-380 nm) for 5 minutes yields cells that are noninfectious as assessed by extended infectivity assays. The p
AIDS Res Hum Retroviruses. 1990 Apr;6(4):553-60. Unique Identifier : AIDSLINE MED/90253943
Furth PA; Westphal H; Hennighausen L; Department of Medicine, University of Maryland Medical School,; Baltimore.
Gene regulation in several retroviral systems is subject to steroid hormone control. Clinical studies have shown that the human immunodeficiency virus (HIV) core protein p24 is more readily detectable in infected women during pregnancy. Here we show that expression of the HIV long terminal repeat (LTR) is induced by g
Cas Lek Cesk. 1990 Mar 16;129(11):343-9. Unique Identifier : AIDSLINE MED/90254755
Strauss J; Burkacka M; Institut hygieny a epidemiologie, Praha.
Cultivation and serological tests for Chlamydia trachomatis were made in a total of 39 male patients (24 married, 11 single, 3 divorced, 1 widower) and their female partners (9 extra-marital, 12 wives) on average 35 years old in both groups. Apart from the clinical diagnosis of non-specific urethritis found in all mal
Anders GT; Timmons JH; Johnson JE; Blanton HM; Hartshorne MF; Pulmonary Disease Service, Brooke Army Medical Center, Fort Sam; Houston, Texas 78234.
The use of gallium scanning in the treatment of patients with AIDS has been well described. In this study, 27 HIV-infected patients (Walter Reed Staging Classification I-V) with normal chest roentgenograms were evaluated to determine the occurrence of thoracic gallium abnormalities in early HIV infection. SPECT was us
Health Psychol. 1990;9(3):253-65. Unique Identifier : AIDSLINE MED/90255458
Doll LS; O'Malley PM; Pershing AL; Darrow WW; Hessol NA; Lifson AR; AIDS Program Center for Infectious Diseases, Centers for Disease; Control, Atlanta, GA 30333.
To evaluate the effectiveness of human immunodeficiency virus (HIV) testing and counseling among homosexual and bisexual men participating in the San Francisco City Clinic Cohort, compared behavioral data from 181 men who learned their HIV antibody status between 1985 and 1987 with data from 128 men who were tested bu
Merelli E; Sola P; Montagnani G; Neurological Department, University of Modena, Italy.
Paired cerebrospinal fluid (CSF) and serum samples from 52 Italian patients affected by myelopathy of unknown origin (MUO) were tested for the presence of antibodies to human T cell lymphotrophic virus type I (HTLV-I) by an enzyme-linked immunosorbent assay, in an attempt to demonstrate a common retroviral origin of M
A national study of the prevalence of HIV antibody in homosexual and heterosexual patients attending sexually transmitted disease clinics in the years 1985-7 was continued in 1988. Among homosexual men in two clinics in south east England HIV antibody was less prevalent in 1988 (30 (7.1%) of 420) than in 1987 (81 (14.
Moyle G; Barton SE; Midgley J; Rowe IF; Keat AC; Lawrence AG; Department of Genitourinary Medicine, Kobler Centre, St Stephen's; Clinic, London, UK.
The development of gonococcal arthritis is reported in a man with HIV infection and CDC Stage IVC2 disease. The diagnosis of disseminated Neisseria gonorrhoeae was facilitated by microbiological examination of a joint aspirate. The auxotype identified by culture was moderately resistant to penicillin, a characteristic
Hillman RJ; Goldmeier D; Taylor-Robinson D; Clinical Research Centre, Harrow, Middlesex, UK.
Four patients with scrotal ulceration were seen during a period of 9 months. All of them were HIV-positive homosexual men with coexisting skin conditions. Herpes simplex virus was not isolated from any of the ulcers, but a variety of bacteria was recovered. It is suggested that multiple factors peculiar to HIV-positiv
Blood histamine levels (BHL; ng histamine base/ml blood, mean +/- SEM) were determined in 118 infants born to HIV-1-infected mothers and in children infected after blood transfusion. In asymptomatic infected infants, BHL were not significantly different from HIV-1-negative infants born to HIV-infected mothers (54.1 +/
Mizuochi T; Matthews TJ; Kato M; Hamako J; Titani K; Solomon J; Feizi T; Division of Biomedical Polymer Science, Fujita Health University,; Aichi, Japan.
The N-linked oligosaccharide structures on the envelope glycoprotein gp120 of human immunodeficiency virus 1 derived from chronically infected lymphoblastoid (H9) cells have been investigated by enzymatic microsequencing after release from protein by hydrazinolysis, labeling with NaB3H4, and chromatography on adsorben
Chow TP; Feldman RA; Lovett M; Piatak M; Genelabs Incorporated, Redwood City, California 94063.
alpha-Trichosanthin (alpha-TCS) is a ribosome-inactivating protein that has recently been shown to inhibit the replication of human immunodeficiency virus. We have isolated a gene encoding alpha-TCS and have determined its DNA sequence. The data indicate that alpha-TCS is synthesized as a preproprotein consisting of 2
Bryson GJ; Keegan RP; Quinn CP; Collins RJ; Department of Pathology, Royal Brisbane Hospital, Herston,; Australia.
The findings of recent surveys indicate a need for standardisation in lymphocyte subset analysis by flow cytometry. Major areas of concern are the methods used for labelling subsets and the choice of appropriate monoclonal antibodies. A standard dual colour manual whole blood lysis technique for flow cytometry was com
Derdiarian AK; Schobel D; Oncology Nursing Graduate Program, School of Nursing, University; of California, Los Angeles.
The major changes resulting from the AIDS diagnosis and illness were measured, described and classified in the Behavioural Systems Model s eight subsystems--achievement, affiliative, aggressiveness/protectiveness, dependence, ingestive, eliminative, restorative and sexual. Thirty male AIDS patients with Kaposi s Sarco
J Immunol. 1990 Jun 1;144(11):4183-8. Unique Identifier : AIDSLINE MED/90257332
Gomatos PJ; Stamatos NM; Gendelman HE; Fowler A; Hoover DL; Kalter DC; Burke DS; Tramont EC; Meltzer MS; Department of Cellular Immunology, Walter Reed Army Institute of; Research, Washington, D.C. 20307-5100.
Macrophages are major viral reservoirs in the brain, lungs, and lymph nodes of HIV-infected patients. But not all HIV isolates infect macrophages. The molecular basis for this restrictive target cell tropism and the mechanisms by which HIV infects macrophages are not well understood: virus uptake by CD4-dependent and
Eeftinck Schattenkerk JK; Lange JM; van Steenwijk RP; Danner SA; Department of Internal Medicine, Academic Hospital of the; University of Amsterdam, The Netherlands.
Fifty consecutive patients with confirmed PCP received a high dose of cotrimoxazole for 14 d, or until development of intolerance, directly followed by reduced dose maintenance therapy. Seven individuals died during the high dose course. Twenty (47%) of the 43 survivors showed toxicity reactions that necessitated dose
J Med Virol. 1990 Mar;30(3):163-8. Unique Identifier : AIDSLINE MED/90257596
Reiss P; Lange JM; de Ronde A; de Wolf F; Dekker J; Debouck C; Goudsmit J; Department of Virology, University of Amsterdam, the Netherlands.
Antibodies to E. coli-produced HIV-1 nef, rev, tat, vpu, and vpr proteins were measured by enzyme immunoassay in serial sets of sera from 72 men seroconverting for antibodies to HIV-1 structural proteins, and from 190 initially symptom-free men who were seropositive for these antibodies at entry into the study. In the
Mazzocchio R; Schieppati M; Scarpini C; Rossi A; Istituto di Scienze Neurologiche, Universita di Siena, Italy.
The recurrent inhibition of the soleus alpha-motoneurons at rest, evaluated by a specially designed method of paired H reflexes, was estimated in 10 patients with spastic paraparesis. In three of these patients, Renshaw cell activity produced inhibition of the corresponding alpha-motor neurons comparable to that obtai
Pentamidine isethionate has been approved by the Food and Drug Administration as a treatment for Pneumocystis carinii pneumonia, the most common opportunistic infection associated with acquired immunodeficiency syndrome. This article outlines the protocol for intravenous administration of pentamidine isethionate in us
J Med Chem. 1990 Jun;33(6):1553-61. Unique Identifier : AIDSLINE MED/90257947
Chu CK; Ullas GV; Jeong LS; Ahn SK; Doboszewski B; Lin ZX; Beach JW; Schinazi RF; Department of Medicinal Chemistry and Pharmacognosy, College of; Pharmacy, University of Georgia, Athens 30602.
In order to study the structure-activity relationships of 2 ,3 -dideoxypurine nucleosides as potential anti-HIV agents, various 6-substituted purine analogues have been synthesized and examined in virus-infected and uninfected human peripheral blood mononuclear cells. N6-methyl-2 ,3 -dideoxyadenosine (D2MeA, 7a) was i
J Med Chem. 1990 Jun;33(6):1663-6. Unique Identifier : AIDSLINE MED/90257961
Warshaw JA; Watanabe KA; Memorial Sloan-Kettering Cancer Center, Sloan-Kettering Division; of Graduate School of Medical Sciences, Cornell University, New; York, New York 10021.
A series of four 2 -azido-2 ,3 -dideoxypyrimidine nucleosides were synthesized and their activity against human immunodeficiency virus was explored. 2,2 -Anhydro-5-O-benzoyluridine (6a) was prepared from 5-O-benzoyluridine (5a) and converted into 3 -deoxy analogue 8a by imidazolylthiocarbonylation followed by Bu3SnH r
J Med Chem. 1990 Jun;33(6):1833-9. Unique Identifier : AIDSLINE MED/90257987
Van Aerschot A; Everaert D; Balzarini J; Augustyns K; Jie L; Janssen G; Peeters O; Blaton N; De Ranter C; De Clercq E; et al; Laboratories of Pharmaceutical Chemistry, Rega Institute for; Medical Research, Leuven, Belgium.
In view of the selective anti-HIV activity of 2 ,3 -dideoxy-3 -fluoro-5-chlorouridine (11), a series of eight 2 ,3 -dideoxy-5-chloropyrimidines were synthesized and evaluated for their inhibitory activity against human immunodeficiency virus type 1 (HIV-1) replication in MT-4 cells. A marked improvement in selectivity
Hammond JS; Eckes JM; Gomez GA; Cunningham DN; Division of Trauma, University of Miami/Jackson Memorial Medical; Center, Florida.
The medical, legal, and ethical problems associated with routine HIV screening have led to the recommendation that all patients should be presumed to be seropositive and thus protective measures should be taken by all health care workers. This philosophy, termed universal precautions, has been difficult to adhere to o
Fabricius EM; Lipp T; Kaboth W; Augenklinik des Stadtischen Akademischen Lehrkrankenhauses; Munchen-Harlaching.
A 43-year-old homosexual man was hospitalized in April 1988 because of acute epigastric pain. It was known that he had had a HIV infection for a year, and in April 1988 it was defined as stage Walter Reed I. Acute, exudative, nonspecific pancreatitis was diagnosed. Three weeks later cerebral symptoms (disturbances of
N Engl J Med. 1990 Jun 7;322(23):1646-51. Unique Identifier : AIDSLINE MED/90259016
Surgenor DM; Wallace EL; Hao SH; Chapman RH; Center for Blood Research, Harvard Medical School, Boston, MA; 02115.
Widespread concern about the safety of the national blood supply, particularly with respect to the human immunodeficiency virus (HIV), has reportedly affected the use of blood products to support patients. To examine these changes, we conducted national surveys of blood collection and transfusion in the
Cox PH; Martin MA; Styer CM; Beall GN; Department of Medicine/Immunology, Harbor-UCLA Medical Center,; Torrance.
Although many experimental treatments are being evaluated for the treatment of acquired immunodeficiency syndrome (AIDS) and symptomatic HIV infection (ARC), only zidovudine ( AZT ) has been shown to prolong the lives of such patients. This article revi
A Committee Opinion on human immunodeficiency virus (HIV) and physician responsibility was developed by The American College of Obstetricians and Gynecologists (ACOG) Committee on Ethics. The committee s conclusion is that the physician s responsibility to care for patients who may have an HIV infection does not diffe
Pathol Res Pract. 1990 Apr;186(2):228-37. Unique Identifier : AIDSLINE MED/90259873
Artigas J; Grosse G; Niedobitek F; Department of Pathology, Auguste-Viktoria-Krankenhaus, Berlin,; FRG.
Between 1986 and 1988 we studied the spinal cord of 40 patients dying of AIDS. Transverse and longitudinal sections from a minimum of four levels of the spinal cord were examined by means of conventional histology, immunohistochemistry and electron microscopy. Out of 22 cases there were 6 showing a mild, 11 a moderate
Chaffey MH; Klein JS; Gamsu G; Blanc P; Golden JA; Department of Radiology, University of California, San Francisco,; School of Medicine 94143.
The radiographic distribution of Pneumocystis carinii pneumonia was studied in 64 consecutive patients with acquired immunodeficiency syndrome to determine the demographic and clinical factors that might be associated with predominance of the disease in the upper zones of the lungs. Twenty-three patients were receivin
Amorosa JK; Nahass RG; Nosher JL; Gocke DJ; Department of Radiology, University of Medicine and Dentistry of; New Jersey, Robert Wood Johnson Medical School, New Brunswick; 08903-0019.
Thirty-four episodes of pyogenic pulmonary infection in 30 patients infected with human immunodeficiency virus (HIV) and 30 episodes of Pneumocystis carinii pneumonia (PCP) in 30 AIDS patients were reviewed to determine if the radiologic features were more helpful than clinical and laboratory findings in the different
Miller WT Jr; Edelman JM; Miller WT; Department of Radiology, Hospital of the University of; Pennsylvania, Philadelphia 19104.
The clinical, laboratory, and radiographic findings in seven patients with acquired immunodeficiency syndrome (AIDS) and cryptococcal pulmonary infections were reviewed. The infection was most commonly seen on radiographs as lymphadenopathy, interstitial infiltrates, or both. Interstitial infiltrates were commonly nod
Carney MD; Combs JL; Waschler W; Department of Ophthalmology, Medical College of Virginia,; Virginia Commonwealth University, Richmond.
Two patients with acquired immune deficiency syndrome presented with headaches and fevers. A diagnosis of cryptococcal meningitis was made by lumbar puncture and elevated cryptococcal antigens. Complaints of decreased vision in both patients led to the diagnosis of optic disc edema and cryptococcal choroiditis with ye
Dummer JS; Department of Medicine and Surgery, University of Pittsburgh,; Pennsylvania 15261.
Pneumocystis carinii is an organism of uncertain taxonomy that causes diffuse pneumonitis in malnourished infants and immunocompromised hosts. In transplant recipients, the infection most commonly presents from 2 to 6 months after transplantation with symptoms of dyspnea, fever, and dry cough lasting from a few days t
In trauma surgery there is a particularly high proportion of patients in the age group most at risk of infection with AIDS. The result of an epidemiological study in our patients (HIV screening of all patients scheduled for surgery at a trauma center over 18 months) showed a prevalence of 0.1%. Specific therapeutic st
The prevalence of HIV-infection was studied in a district hospital in Botswana , in southern Africa. Sera from 466 patients were analysed. The sexually transmitted diseases (STD) group consisted of 175 women and 178 men, who consulted the STD-clinic for complaints which could be a
Infection of Molt-3 cells with human immunodeficiency virus-1 (HIV-1) was found to cause a rapid increase in extractable poly(A) polymerase activity, while the activity of poly(A) degrading endoribonuclease IV strongly decreased at the same time. The increase in poly(A) polymerase activity seems not to be due to a cha
Pokrovskii VV; Eramova IIu; Arzamastsev VP; Nikonova VA; Mozharova GI
The existing system of the epidemiological surveillance of HIV infection in the USSR makes it possible to evaluate the present situation as the beginning of epidemic. The main routes of the transfer of HIV infection as per data for April 1989: 50% by the sexual route, 25.8% by parenteral manipulations.
In 58 persons infected with HIV the comparative study of the content of immunocompetent cells was carried out by the methods of rosette formation and immunofluorescence with the use of monoclonal antibodies. An increase in the content of rosette-forming cells (RFC), such as active E-RFC, E-RFC, theophylline-resistant
Stevens JR; Papadopoulos NM; Resnick M; Department of Neurology, Oregon Health Sciences University,; Portland.
Numerous immunologic abnormalities have been reported in patients with schizophrenia, suggesting that this disorder may in some cases represent a viral or immunologic disorder of the brain. To further examine this question, evidence for intrathecal production of immunoglobulin (IgG) and immunoglobulin oligoclonal band
Magura S; Shapiro JL; Grossman JI; Siddiqi Q; Lipton DS; Amann KR; Koger J; Gehan K; Long Island Jewish/Hillside Medical Center.
The emotional and behavioral responses of methadone patients to HIV antibody testing were examined by comparing 8 seropositives with 8 matched seronegatives. Seropositives displayed a wide range of immediate emotional reactions to learning their results, but no severe or damaging reactions; seronegatives were uniforml
Tricoire J; Robert A; Puel J; Boneu B; Gayet C; Regnier C; Service de Medecine Infantile B, CRTS, CHU Purpan, Toulouse.
In order to investigate the progression to AIDS, we studied 39 patients with hemophilia ranging presently in age from 8 to 20 years, who had been positive for HIV antibodies for a mean of 36 months. Every 3 to 6 months a clinical and laboratory examination was performed. The number of patients with AIDS increased from
Neveu I; Labrune P; Huguet P; Musset D; Chaussain JL; Odievre M; Service de Pediatrie, Hopital Antoine-Beclere, Clamart.
The case of a 3 year-old boy who presented isolated cholestasis as the initial symptom of histiocytosis X is described. Other symptoms occurred later on. Despite treatment (corticosteroid and Vinblastin), the disease continued to progress and the child died at the age of 4 1/2 years with HIV infection (contaminated bl
Aust N Z J Med. 1990 Apr;20(2):141-8. Unique Identifier : AIDSLINE MED/90262489
Edwards P; Wodak A; Cooper DA; Thompson IL; Penny R; Bruce Hall Dept of Gastroenterology, St Vincent's Hospital,; Sydney, NSW, Australia.
Gastrointestinal disease in AIDS is common and is due to opportunistic infections, aggressive malignancy and possible direct HIV enteropathy. Disabling gastrointestinal symptoms are prominent both in patients with established AIDS and in patients with earlier stages of HIV infection. We report the cases of 160 patient
Ayres L; Avillez F; Garcia-Benito A; Deinhardt F; Gurtler L; Denis F; Leonard G; Ranger S; Grob P; Joller-Jemelka H; et al; National Institute of Health, Lisbon, Portugal.
A newly developed recombinant antigen-based anti-HIV-1/HIV-2 enzyme immunoassay (Abbott Recombinant HIV-1/HIV-2 EIA) was evaluated against a second generation anti-HIV-1 EIA (Abbott Recombinant HIV-1 EIA). Five thousand and twenty-nine sera from European blood donors and 403 sera from central African blood donors were
This study initiates a new method of developing an antigen which might be useful in the prevention of HIV-1 infection. Using a mannan preparation from Saccharomyces cerevisiae neutralizing antiserum was raised in rabbits which prevents HIV-1 infection in vitro up to a titre of 1:128. The corresponding antibody prepara
AIDS Res Hum Retroviruses. 1990 Feb;6(2):251-60. Unique Identifier : AIDSLINE MED/90226894
Piedimonte G; Petronini PG; Guetard D; Favier V; Borghetti AF; Montagnier L; Istituto di Patologia Generale, University of Parma, Italy.
The mechanism of cytopathic effects associated with HIV infection in a continuous line of CD4-positive lymphocytes (CEM cells, clone 13) has been studied. Here we report the following observations: (1) HIV infection killed a variable but always significant number of cells without a strict relationship with the syncyti
EMBO J. 1990 May;9(5):1551-60. Unique Identifier : AIDSLINE MED/90228355
Stevenson M; Stanwick TL; Dempsey MP; Lamonica CA; Department of Pathology and Microbiology, University of Nebraska; Medical Center, Omaha 68105.
During progression of the Acquired Immune Deficiency Syndrome (AIDS), the human immunodeficiency virus type 1 (HIV-1) is harbored in CD4+ T cells, which act as the primary reservoir for the virus. In vitro, HIV-1 requires activated T cells for a productive infection; however, in vivo, the number of circulating T cells
Hospitals. 1990 May 5;64(9):26-34. Unique Identifier : AIDSLINE MED/90228940
Larkin H; Koska MT; Hudson T; Eybanks P
Jackson Memorial Hospital, Miami, lost $5.6 million treating AIDS patients last year. Executives at the 1,246-bed public hospital project losses of more than $7 million in 1990. Jackson Memorial s experience is unfortunately all too typical of the effect that AIDS has had on public hospitals. But AIDS is not just a pu
Int J Immunopharmacol. 1990;12(2):145-7. Unique Identifier : AIDSLINE MED/90229140
Hording M; Gotzsche PC; Bygbjerg IC; Christensen LD; Faber V; Department of Infectious Diseases, Rigshospitalet, Copenhagen,; Denmark.
Disulfiram (Antabuse (R)) is metabolized to two molecules of diethyldithiocarbamate, which has been reported to be an immunomodulating agent. In a double blind trial, 15 HIV antibody positive homosexual men were given daily doses of 100 mg or 400 mg of disulfiram or placebo, for 4 weeks. All had a CD4-count below 500
JAMA. 1990 May 9;263(18):2510-4. Unique Identifier : AIDSLINE MED/90230409
The DATTA panelists considered aerosolized pentamidine to be both safe and effective for primary and secondary prophylaxis of PCP. T4 helper cell counts offer guidance as to the best candidates for primary prophylaxis. Patients with a T4 helper cell count of fewer than 200/mm3 are the most appropriate group to receive
The prevalence of antibodies against HIV-1 regulatory proteins in sera of HIV-infected patients from different stages of disease was investigated. HIV-1 vif, tat, and nef genes were cloned in procaryotic vectors and were expressed as MS-2 fusion proteins (vif and nef) or as a non-fusion protein (tat). These recombinan
Takahashi H; Takeshita T; Morein B; Putney S; Germain RN; Berzofsky JA; Molecular Immunogenetics and Vaccine Research Section, National; Cancer Institute, National Institutes of Health, Bethesda,; Maryland 20892.
To reduce the risks of immunization with killed or live attenuated virus vaccines, it may be advantageous to use a pure, defined antigen that contains determinants for both humoral and cellular immunity. However, although most non-living intact protein preparations induce antibodies and CD4+ major histocompatibility c
Nature. 1990 May 3;345(6270):84-6. Unique Identifier : AIDSLINE MED/90231470
Ensoli B; Barillari G; Salahuddin SZ; Gallo RC; Wong-Staal F; Laboratory of Tumor Cell Biology, National Cancer Institute,; National Institutes of Health, Bethesda, Maryland 20892.
Kaposi s sarcoma (KS) is frequently associated with human immunodeficiency virus-1 (HIV-1) infection. Supernatants from HIV-1-infected T cells carrying the CD4 antigen promote the growth of cells derived from KS lesions of AIDS patients (AIDS-KS cells), and the HIV-1 tat gene, introduced into the germ line of mice, in
Tonn BE; Travis CB; Goeltz RT; Phillippi RH; Oak Ridge National Laboratory, Tennessee 37831.
Beliefs about risks associated with two risk agents, AIDS and toxic waste, are modeled using knowledge-based methods and elicited from subjects via interactive computer technology. A concept net is developed to organize subject responses concerning the consequences of the risk agents. It is found that death and advers
Somma-Mauvais H; Regis H; Gastaut JL; Gastaut JA; Farnarier G; Service d'Explorations Fonctionnelles du Systeme Nerveux,; Hopital de La Timone, Marseille.
We have studied 95 HIV seropositive patients (77 males and 18 females; mean age: 31 years): 67 had no neurological symptoms or signs, 28 had various neurological symptoms and signs. This study included a full multimodal evoked potentials (MEP) assessment: visual evoked potentials by flash and reversal checkerboard; br
Friedlander WJ; Department of Preventive and Societal Medicine, University of; Nebraska College of Medicine, Omaha.
This study examines the historical bases of what physicians ought to do as well as what they actually have done with regard to the treatment of diseases such as AIDS, which put them at risk for their lives. The earliest explicit statement of what ought to be done goes back only to 1847, when the
Buskila D; Gladman D; Rheumatic Disease Unit, University of Toronto, Women's College; Hospital, Ontario, Canada.
Musculoskeletal manifestations and autoimmune phenomena are recently recognized complications of infection with human immunodeficiency virus (HIV). Patients with HIV infection share several clinical and serologic features with patients with systemic lupus erythematosus and Sjogren s syndrome; these similarities may le
Gallis HA; Drew RH; Pickard WW; Division of Infectious Diseases, Duke University Medical Center,; Durham, North Carolina 27710.
Amphotericin B, the first commercially significant antifungal drug, has been available for more than 30 years. This polyene macrolide antifungal agent continues to play a major role in the treatment of systemic fungal infections, despite the introduction of newer agents such as the azoles. Given the proved efficacy of
Hay RJ; Department of Clinical Sciences, London School of Hygiene and; Tropical Medicine, United Kingdom.
Studies with fluconazole in oropharyngeal candidiasis have focused primarily on three groups of infections: chronic atrophic candidiasis, oropharyngeal infections associated with either neutropenia or AIDS, and chronic mucocutaneous candidiasis. In two studies of chronic atrophic candidiasis associated with dentures,
Sugar AM; Stern JJ; Dupont B; Evans Memorial Department of Clinical Research, Boston University; Medical Center, Massachusetts.
Infections caused by Cryptococcus neoformans cause significant morbidity and high mortality, particularly among immunocompromised patients. Cryptococcal meningitis is an important cause of central nervous system disease and death in patients with AIDS. Although the i
Salud Publica Mex. 1990 Jan-Feb;32(1):15-9. Unique Identifier : AIDSLINE MED/90232373
Leo-Amador GE; Ramirez-Rodriguez JM; Galvan-Villegas F; Perla-Navarro AV; Servicio de Medicina Interna, Hospital de Especialidades,; Instituto Mexicano del Seguro Social, Guadalajara, Jalisco.
After studying a bisexual male with a clinical picture suggestive of AIDS a positive ELISA test for antibodies against the Human Immunodeficiency Virus (HIV), but negative results on indirect immunofluorescence testing, as well, as absence of core and envelope HIV antibodies by ELISA, and who later turned out to have
Subramanian T; Kuppuswamy M; Venkatesh L; Srinivasan A; Chinnadurai G; Institute for Molecular Virology, St. Louis University School of; Medicine, Missouri 63110.
The tat gene of HIV is a strong activator of the viral LTR. The Tat protein contains a highly basic domain that is important for its transport to the nuclear/nucleolar locations. The Tat basic domain when fused to Escherichia coli beta-galactosidase directed the chimeric protein to the nucleus and nucleolus. Tat mutan
Ma XY; Sakai K; Sinangil F; Golub E; Volsky DJ; Molecular Virology Laboratory, St. Luke's/Roosevelt Hospital; Center, New York, New York.
Recently described noncytopathic human immunodeficiency viruses type-1 (HIV-1) form a new category within the HIV-1 family due to their unique biological properties and predominant occurrence in symptom-free individuals. To study the mechanism of noncytopathic HIV-1 infection, we compared the infectivity and life cycl
Sainz Corton E; Torreblanca Pacheco M; Garcia Reyes S; Servicio de Psiquiatria II del Hospital General Gregorio; Maranon, Madrid.
88 patients presenting HIV seropositivity are studied at the Psychiatric Intersurgery of the Gregorio Maranon General Hospital in Madrid. All of them but 4 are drug addicts having acquired the illness through homo or heterosexual relations. After a wide bibliographical survey of the psychologic ann psychiatric aspects
Annu Rev Med. 1990;41:159-68. Unique Identifier : AIDSLINE MED/90233706
Bridge TP; Ingraham LJ; Division of Intramural Research, National Institute of Mental; Health, Bethesda, Maryland 20892.
Following its early entry into the central nervous system (CNS), HIV-1 alters cerebral cell architecture and may subsequently affect higher cognitive functions, leading eventually in some patients to HIV-1 encephalopathy. The CNS may also be the target of opportunistic infections and malignancy secondary to HIV-1 immu
Annu Rev Med. 1990;41:339-47. Unique Identifier : AIDSLINE MED/90233723
Brasitus TA; Sitrin MD; Department of Medicine, University of Chicago, Illinois 60637.
This chapter is a review of selected causes of intestinal malabsorption. The pathogenesis and clinical features of malabsorption due to acquired immunodeficiency syndrome (AIDS), celiac sprue, and small bowel bacterial overgrowth are discussed, with emphasis on recent developments. The etiology and diagnostic challeng
Cancer Genet Cytogenet. 1990 May;46(1):9-19. Unique Identifier : AIDSLINE MED/90235188
Hecht F; Hecht BK; Genetics Center and Cancer Center of Genetrix, Inc., Scottsdale,; Arizona.
A gene locus for ataxia-telangiectasia (A-T) is in chromosome region 11q22 to 11q23 and predisposes to cancer. Ataxia-telangiectasia patients appear to have two separate clinical patterns of malignancy. One pattern involves solid tumors, which have not been stressed and which include malignancies in the oral cavity, b
We have characterized the immunoreactivity to human T-cell lymphotropic virus type I (HTLV-I) among 26,983 persons of various seroprevalence groups by using enzyme immunoassay, immunoblot (IB), and radioimmunoprecipitation assays (RIPA) in accordance with Public Health Service recommended guidelines for the interpreta
J Am Podiatr Med Assoc. 1990 Mar;80(3):127-34. Unique Identifier : AIDSLINE MED/90237971
Cohen EJ; Cole D; Stewart DM; Weiss G; Kosinski M; Giorgini R; Division of Orthopedics, New York College of Podiatric Medicine,; NY 10035.
The authors review four cases of Kaposi s sarcoma that were presented to the Foot Clinics of New York and affiliated North General Hospital during a 1-year period from the fall of 1987 to the fall of 1988. The authors conclude that it is sometimes difficult to diagnose Kaposi s sarcoma and to differentiate between the
Md Med J. 1990 Apr;39(4):365-9. Unique Identifier : AIDSLINE MED/90238381
Chaisson RE; Smith MD; AIDS Service, Johns Hopkins Hospital, Baltimore.
As management of HIV infection becomes more proactive, early identification of persons at risk for PCP and initiation of preventive therapy will become more routine, and the clinical impact of P. carinii may be ameliorated.
Schiodt M; Bakilana PB; Hiza JF; Shao JF; Bygbjerg IB; Mbaga I; Vestergaard BF; Nielsen CM; Lauritzen E; Lerche B; et al; Department of Oral Medicine and Oral Surgery, University; Hospital, Copenhagen.
We report a detailed study on oral lesions and their association with the WHO revised provisional case definition of AIDS as well as serologic signs of HIV infection among 186 patients in Dar Es Salaam, Tanzania . The patient material consisted of 39 hospitalized suspected AIDS pa
Goodman P; Maklad NF; Verani RR; Gottlieb HE; Department of Radiology, University of Texas Medical School,; Houston.
A patient with acquired immune deficiency syndrome (AIDS) who presented with right testicular swelling is described. Sonography demonstrated diffuse enlargement and inhomogeneity of the testicle with central hypoechoic areas. Testicular abscesses were seen at surgery and Mycobacterium tuberculosis was cultured from th
Wray D; Felix DH; Cumming CG; Department of Oral Medicine and Oral Pathology, University of; Edinburgh.
The objectives of this preliminary study were to determine the prevalence of oral candidal carriage and infection in a group of HIV-positive individuals and compare the humoral immune responses in serum and saliva in this group with a control group of HIV-negative subjects. Patients were examined clinically with parti
Int J Cancer. 1990 May 15;45(5):865-8. Unique Identifier : AIDSLINE MED/90243370
Kuroda N; Washitani Y; Shiraki H; Kiyokawa H; Ohno M; Sato H; Maeda Y; Fukuoka Red Cross Blood Center, Japan.
We describe an enzyme-linked immunosorbent assay (ELISA) for antibodies to HTLV-I using synthetic peptides corresponding to antigenic regions of HTLV-I structural protein. We observed that the peptides amino acid 100-119, 100-130, 131-160 and 295-314 of the gag protein, and 89-115, 175-199, 350-386 and 458-488 on the
Int J Cancer. 1990 May 15;45(5):980-3. Unique Identifier : AIDSLINE MED/90243390
Iwahara Y; Takehara N; Kataoka R; Sawada T; Ohtsuki Y; Nakachi H; Maehama T; Okayama T; Miyoshi I; Department of Medicine, Kochi Medical School, Japan.
Four rabbits inoculated intravenously with milk cells from 4 post-partum women seropositive for human T-cell leukemia virus type I (HTLV-I) and one rabbit inoculated with semen cells from a seropositive healthy man seroconverted for HTLV-I after 3-5 weeks but no seroconversion occurred in 2 rabbits inoculated with mil
Richards AD; Phylip LH; Farmerie WG; Scarborough PE; Alvarez A; Dunn BM; Hirel PH; Konvalinka J; Strop P; Pavlickova L; et al; Department of Biochemistry, University of Wales College of; Cardiff, United Kingdom.
By replacement of the P1 residue in a capsid/nucleocapsid cleavage site mimic with 4-NO2-phenylalanine (Nph), an excellent chromogenic substrate, Lys-Ala-Arg-Val-Leu*Nph-Glu-Ala-Met, for HIV-1 proteinase (kappa cat = 20 s-1, Km = 22 microM) has been prepared. Substitution of the Leu residue in P1 with norleucine, Met
Nyborg JK; Matthews MA; Yucel J; Walls L; Golde WT; Dynan WS; Wachsman W; Department of Chemistry and Biochemistry, University of Colorado,; Boulder 80309.
We present a map describing the binding of cellular proteins to a 300-base pair (bp) region of the human T-cell leukemia virus type I (HTLV-I) long terminal repeat. The map accounts for nearly all of the DNase I protection reported in a previous study using crude nuclear extracts. Notable features include a complex ar
Meichle A; Schutze S; Hensel G; Brunsing D; Kronke M; Klinische Arbeitsgruppe der Max-Planck-Gesellschaft, Gottingen,; Federal Republic of Germany.
Tumor necrosis factor (TNF) is one of the few physiological inducers of the pleiotropic transcription factor NF kappa B. NF kappa B may play a central role in mediating TNF s gene regulatory action; however, the molecular mechanisms of TNF-induced NF kappa B activation are poorly understood. In this study, we demonstr
Benko DM; Schwartz S; Pavlakis GN; Felber BK; National Cancer Institute-Frederick Cancer Research Facility,; Maryland 21701-1013.
We have characterized a novel 28-kilodalton protein, p28tev, detected in human immunodeficiency virus type 1-infected cells. tev is recognized by both tat and rev monospecific antibodies. tev is initiated at the tat AUG and contains the first exon of tat at its amino terminus, a small portion of env in the middle, and
Weaver TA; Talbot KJ; Panganiban AT; McArdle Laboratory for Cancer Research, University of; Wisconsin-Madison 53706.
The nature of spleen necrosis virus pol gene expression and the role of gag and gag-pol polyproteins in virion assembly was investigated. The DNA sequence of the gag-pol junction revealed that the two genes occupy the same open reading frame but are separated by an in-frame amber stop codon. Biochemical analysis of ga
Haffar O; Garrigues J; Travis B; Moran P; Zarling J; Hu SL; Department of Virology, Oncogen, Seattle, Washington 98121.
We report the assembly of human immunodeficiency virus (HIV)-like particles in African green monkey kidney cells coinfected with two recombinant vaccinia viruses, one carrying the HIV-1 gag and protease genes and the other the env gene. Biochemical analysis of particles sedimented from culture supernatants of doubly i
Since human immunodeficiency virus (HIV) nef has been suggested to exert regulatory effects on HIV long terminal repeat (LTR) activity, we transiently transfected HIV LTR chloramphenicol acetyltransferase or luciferase expression vectors into a human astrocytoma clone (U-373nef) that constitutively expresses the HIV n
Chun R; Glabe CG; Fan H; Department of Molecular Biology and Biochemistry, University of; California, Irvine 92717.
Full-length (86-residue) polypeptide corresponding to the human immunodeficiency virus type 1 tat trans-activating protein was chemically synthesized on a semiautomated apparatus, using an Fmoc amino acid continuous-flow strategy. The bulk material was relatively homogeneous, as judged by sodium dodecyl sulfate-polyac
Haffar OK; Nakamura GR; Berman PW; Department of Developmental Biology, Genetech Inc., South San; Francisco, California 94080.
Mutagenesis of the transmembrane domain and cytoplasmic tail of human immunodeficiency virus type 1 envelope glycoprotein gp160 revealed that its intracellular transport and processing in transfected cell lines were modulated by a functional domain included in the carboxy-terminal sequence consisting of residues 751 t
N Engl J Med. 1990 May 24;322(21):1488-93. Unique Identifier : AIDSLINE MED/90245039
Fischl M; Galpin JE; Levine JD; Groopman JE; Henry DH; Kennedy P; Miles S; Robbins W; Starrett B; Zalusky R; et al; University of Miami, Fla.
Bone marrow suppression and anemia are frequent side effects of treatment of the acquired immunodeficiency syndrome (AIDS) with zidovudine (formerly azidothymidine [ AZT ]). We conducted a randomized, double-blind, placebo-controlled clinical trial of r
Meyerhans A; Vartanian JP; Wain-Hobson S; Laboratoire de Biologie et Immunologie Moleculaires des; Retrovirus, Institut Pasteur, Paris, France.
PCR co-amplification of two distinct HIV1 tat gene sequences lead to the formation of recombinant DNA molecules. The frequency of such recombinants, up to 5.4% of all amplified molecules, could be decreased 2.7 fold by a 6 fold increase in Taq DNA polymerase elongation time. Crossover sites mapped essentially to three
Berkhout B; Gatignol A; Silver J; Jeang KT; Laboratory of Molecular Microbiology, National Institute of; Allergy and Infectious Diseases, Bethesda, MD 20892.
Human immunodeficiency viruses HIV-1 and HIV-2 encode a Tat protein that activates transcription from the long terminal repeats. The target for transactivation is termed the trans-acting responsive (TAR) element. TAR has an extensively folded RNA secondary structure and is present at the 5 end of all viral mRNAs. Con
Mermer B; Felber BK; Campbell M; Pavlakis GN; National Cancer Institute-Frederick Cancer Research Facility,; BRI-Basic Research Program, MD 21701-1013.
A synthetic rev gene containing substitutions which introduced unique restriction sites but did not alter the deduced amino acid sequence was used as a vehicle to construct mutations in rev. Insertion or substitution mutations within a domain of Rev resulted in proteins able to inhibit the function of Rev protein in t
Alessandrini A; Canessa A; Mantia E; Artioli S; del Bono V; de Leo P; Pagano G; Terragna A; 1a Clinica delle malattie infettive dell'Universita, Genova,; Italy.
The personal experience on neurological disturbances associated with early HIV infection and AIDS is reported. Central nervous system (CNS) involvement occurred during the early stages in 3 cases: 2 patients with HIV-seroconversion (CDC category III) and one patient with persistent generalized lymphadenopathy (PGL, II
Imberciadori G; Piersantelli N; Bo A; Calderisi S; Emanuelli F; Marchetto E; Penco G; Farinelli M; Arata L; Ribizzi G; et al; Institute of Hygiene, University of Genoa.
Gallo P; Laverda AM; De Rossi A; Pagni S; Cogo P; Argentiero V; Francavilla E; Piccinno MG; del Mistro A; Cadrobbi P; et al; University of Padova, School of Medicine, Institute of Neurology.
Am J Med. 1990 May 21;88(5B):8S-10S. Unique Identifier : AIDSLINE MED/90247438
Richman DD; Department of Pathology, University of California, San Diego.
The emergence of human immunodeficiency virus resistant to 3 -azido-3 -deoxythymidine (zidovudine, AZT ) in patients with acquired immunodeficiency syndrome (AIDS) or AIDS-related complex has been documented. Isolates from non-AZT-treated persons or th
Raviglione MC; Mariuz P; Pablos-Mendez A; Battan R; Ottuso P; Taranta A; Department of Medicine, Cabrini Medical Center, New York, NY; 10003.
Staphylococcus aureus has been reported to cause a high number of infections and septicemias, often related to intravenous catheters, in patients with acquired immunodeficiency syndrome (AIDS). Our objective was to assess the frequency of S. aureus nasal carriage among patients with AIDS or AIDS-related complex (ARC).
Sirianni MC; Tagliaferri F; Aiuti F; Department of Allergy and Clinical Immunology, University of Rome; La Sapienza, Italy.
Deficiency in natural killer (NK) cell activity is a common feature of acquired immune deficiency syndrome (AIDS). This is part of a general immune dysfunction in AIDS and may lead to progression of the disease, since NK cells are known to be involved in protection against tumors and against viral infections. The lack
Rosenberg ZF; Fauci AS; National Institute of Allergy and Infectious Diseases, National; Institutes of Health, Bethesda, MD 20892.
In this paper Zeda Rosenberg and Anthony Fauci review the prevailing hypotheses on the mechanisms by which human immunodeficiency virus (HIV) progressively and relentlessly destroys immune function in infected individuals. Although HIV can directly kill CD4+ T cells in vitro, the protracted course of HIV infection in
Montaner JS; Le T; Fanning M; Gelmon K; Tsoukas C; Falutz J; O'Shaughnessy M; Wainberg MA; Ruedy J; Department of Medicine, St. Paul's Hospital, University of; British Columbia, Vancouver, Canada.
Seventy-four HIV-infected homosexual males belonging to CDC groups IIB, III, and IVC2 were treated with increasing doses of zidovudine within the Multicentre Canadian AZT Trial. Following a 3 week observation period, consenting volunteers received 600
Andreassen H; Bohr H; Bohr J; Brunak S; Bugge T; Cotterill RM; Jacobsen C; Kusk P; Lautrup B; Petersen SB; et al; Laboratory for Protein Chemistry, University of Copenhagen,; Denmark.
A neural network computer program, trained to predict secondary structure of proteins by exposing it to matching sets of primary and secondary structures from a database, was used to analyze the human immunodeficiency virus (HIV) proteins p17, gp120, and gp41 from their amino acid sequences. The results are compared t
Proc Natl Acad Sci U S A. 1990 May;87(10):3933-7. Unique Identifier : AIDSLINE MED/90251671
Baldwin GC; Fleischmann J; Chung Y; Koyanagi Y; Chen IS; Golde DW; Division of Hematology-Oncology, UCLA School of Medicine 90024.
There is compelling clinical evidence for dysfunction of the mononuclear phagocyte system in patients with AIDS, which is believed due in part to loss of T-cell cooperativity. The direct consequences of human immunodeficiency virus infection on macrophage function are unknown. To address this question we infected norm
Proc Natl Acad Sci U S A. 1990 May;87(10):3997-4001. Unique Identifier : AIDSLINE MED/90251684
Weiser B; Peress N; La Neve D; Eilbott DJ; Seidman R; Burger H; Department of Medicine, State University of New York, Stony Brook; 11794.
To investigate human immunodeficiency virus type 1 (HIV-1) pathogenesis in infected individuals and examine the correlation of HIV-1 expression with extent of clinical and pathologic disease, we studied spinal cords from acquired immunodeficiency syndrome patients with a wide range of spinal cord pathology. By perform
de la Cruz CA; de Diego R; Canal MI; Cabrerizo P; Tisner M; Navia J; Servicio de Anestesiologia y Reanimacion, Hospital General; Gregorio Maranon, Madrid.
Schweiz Rundsch Med Prax. 1990 May 2;79(18):557-61. Unique Identifier : AIDSLINE MED/90251869
Vonwiller HM; Kantonsspital, Luzern.
HIV-infected patients are prone to frequent opportunistic infections (OI). Their fundamental differences to infections in the immunocompetent host are explained. The most frequent OI is pneumonia caused by pneumocystis carinii. A brief overview on diagnostic and therapeutic aspects of encephalitis due to toxoplasma go
Neuropathological lesions were studied in a consecutive autopsy series of 206 cases, comprising 61% of all patients who died of Aids in Switzerland between April 1981 and December 1988. Central nervous system involvement was found in 84% of the patients, and 17% showed multipl
von Allmen M; Bastard B; Cardia-Voneche L; Languin N; Institut de medecine sociale et preventive, Universite de; Geneve.
This article reviews the literature on choice of partner, with the aim of stimulating though about the aids prevention. The criteria involved in choosing a partner are discussed, and a model describing the process by which a couple meet, continue to see one another, and finally decide to live together is presented. Th
Am J Trop Med Hyg. 1990 May;42(5):460-4. Unique Identifier : AIDSLINE MED/90252913
Schindzielorz AH; Belshe RB; Mufson MA; Marshall University School of Medicine, Huntington, West; Virginia.
To further characterize HIV-1 and HIV-2 Western blot indeterminate (IWB) sera, 402 sera from 318 healthy low-risk individuals from West Virginia and 159 African sera obtained in the pre-AIDS era (1968-1972) were studied. All IWB sera tested for antigen by HIV-1 enzyme immunoassay (EIA-Ag) were negative. HIV-1 and HIV-
Ferdinand FJ; Paul-Ehrlich-Institut, Bundesamt fur Sera und Impfstoffe,; Langen, Fed. Rep. of Germany.
In designing antiviral drugs and therapeutic schemes some basic considerations should be taken into account: 1. RNA viruses and especially HIV (Human Immunodeficiency Virus) respond to environmental changes with evading mutations, hence, the high degree of variability of these viruses. All drugs interfering with viral
1) The aspartic proteinase of the human immunodeficiency virus type 1 (HIV-1) was purified from cultures of recombinant E. coli. The enzyme preparation is homogeneous as judged by SDS-polyacrylamide gel electrophoresis and isoelectric focusing. 2) A rapid assay procedure for the proteinase was established which makes
AIDS Res Hum Retroviruses. 1990 Mar;6(3):299-305. Unique Identifier : AIDSLINE MED/90253918
Chirmule N; Kalyanaraman VS; Saxinger C; Wong-Staal F; Ghrayeb J; Pahwa S; Department of Pediatrics, North Shore University Hospital-Cornell; University Medical College, Manhasset, NY.
Patients with AIDS are known to have B-cell hyperactivity. We have previously demonstrated that an extract of HIV-1 could induce differentiation of peripheral blood B lymphocytes of healthy volunteers into immunoglobulin-secreting cells. In an attempt to delineate the B-cell stimulatory subregion in HIV-1, we have inv
We have previously shown that the cell line 6D5(451) chronically infected with the HIV-1 isolate HTLV-III(451), secretes the HIV-1 envelope glycoproteins gp120 and gp160 in the extracellular medium. The HTLV-III(451) gp120 and gp160 were purified by sequential affinity chromatographic steps using a monoclonal antibody
A truncated molecule containing the N-terminal 183 amino acid residues of CD4 (sCD4-183) has been produced in Escherichia coli at high levels, using the trp promoter and an AT-rich ribosome binding site to direct expression in a pBR322-derived vector. A culture has been selected which allows large-scale fermentation a
AIDS Res Hum Retroviruses. 1990 Apr;6(4):525-34. Unique Identifier : AIDSLINE MED/90253940
Zuber M; Samuel KP; Lautenberger JA; Kanki PJ; Papas TS; Program Resources, Inc., Frederick, MD.
Five unique recombinant polypeptides, each encoded by a DNA segment representing a different region of the HIV-2 (NIH-Z strain) env gene, were produced at relatively high levels (greater than or equal to 5%) as cII-fusion products in Escherichia coli. These recombinant polypeptides were characterized serologically by
AIDS Res Hum Retroviruses. 1990 Apr;6(4):535-42. Unique Identifier : AIDSLINE MED/90253941
Tacket CO; Baqar S; Munoz C; Murphy JR; Department of Medicine, University of Maryland School of; Medicine, Baltimore 21201.
The protective immune response to human immunodeficiency virus type 1 (HIV-1) induced by vaccination will likely include cellular immune responses. We measured lymphoproliferative responses in persons vaccinated with a baculovirus-derived recombinant gp160 candidate AIDS vaccine. Twelve volunteers received either 40 m
A simple Escherichia coli system has been developed for the detection of human immunodeficiency virus (HIV) protease activity. In this system, the protease sequence is placed downstream of the HIV gag polypeptide in an operon arrangement. Upon expression of the operon, gag serves as the substrate for the protease; the
AIDS Res Hum Retroviruses. 1990 Apr;6(4):561-5. Unique Identifier : AIDSLINE MED/90253944
Kwok S; Gallo D; Hanson C; McKinney N; Poiesz B; Sninsky JJ; Department of Infectious Diseases, Cetus Corporation, Emeryville,; CA.
The polymerase chain reaction (PCR) was used to confirm the presence of human T-cell lymphotropic viruses (HTLV) in intravenous drug users (IVDU) whose sera were reactive by immunofluorescence assay (IFA) for HTLV-1/-II antibody. Peripheral blood mononuclear cells from 41 IFA-positive and 19 IFA-negative individuals w
FEMS Microbiol Lett. 1990 Mar 15;56(3):233-8. Unique Identifier : AIDSLINE MED/90255919
Hay PE; Clarke JR; Strugnell RA; Taylor-Robinson D; Goldmeier D; Clinical Research Centre, Harrow, Middlesex, London.
The polymerase chain reaction was used to detect Treponema pallidum in specimens of cerebrospinal fluid (CSF), as a means of diagnosing syphilis. Segments of the TmpA and 4D genes were amplified to provide an estimated threshold sensitivity of approximately 65 organisms in 0.5 ml. A spectrum of pathogens known to caus
J Immunol. 1990 Jun 1;144(11):4202-11. Unique Identifier : AIDSLINE MED/90257335
Tanaka Y; Tozawa H; Koyanagi Y; Shida H; Department of Immunology, School of Hygienic Sciences, Kitasato; University, Kanagawa, Japan.
We established rat T cell lines expressing human T cell leukemia virus type I (HTLV-I) Ag from inbred strains of rats, WKA/H, DA, and F344, to study CTL response against the HTLV-I-infected cells. HTLV-I-specific Ag expressed in these rat cells were HTLV-I gag Ag, p19, p24, and p15, and pX Ag, p40tax and p27rex, but n
J Med Virol. 1990 Mar;30(3):206-10. Unique Identifier : AIDSLINE MED/90257605
Tardy JC; Basson J; Douglas A; Aymard M; Departement d'Etude des Maladies Virales, Laboratoire National; de la Sante, Lyon, France.
A blocking ELISA was developed to confirm the specificity of screening tests for anti-HIV-1 antibodies. A murine monoclonal antibody (McAb) raised against recombinant gp160 was used in combination with a commercial technique (ELA-VIA-1). After determining the optimal experimental conditions, the assay was applied to 9
Mandel ID; Columbia University, School of Dental and Oral Surgery, New York,; New York 10032.
It is becoming increasingly apparent to investigators and clinicians in a variety of disciplines that saliva has many diagnostic uses and is especially valuable in the young, the old and infirm and in large scale screening and epidemiologic studies. The highly sensitive test procedures that are now commonplace makes i
Nature. 1990 May 24;345(6273):356-9. Unique Identifier : AIDSLINE GENBANK/X52154
Huet T; Cheynier R; Meyerhans A; Roelants G; Wain-Hobson S; Laboratoire de Biologie et Immunologie Moleculaires des; Retrovirus, Institut Pasteur, Paris, France.
Simian immunodeficiency viruses have been isolated from four species of monkey, the captive macaque and mangabey and the feral African green monkey and mandrill. While none of these viruses is a replica of HIV-1, the macaque and mangabey viruses represent correct genetic models for HIV-2, possessing exactly the sa
De Mercato R; Perna E; Oriani G; Siciliano V; Iovinella V; Ciampi R; Bassi A; Tagliaferro R; II Facolta di Medicina, Universita degli Studi di Napoli.
Serum beta 2-microglobulin levels (beta-2-M) were studied in 150 drug addicts, 50 of them asymptomatic carriers of anti HIV-1 antibodies, 50 symptomatic carriers with persistent generalized lymphadenopathy (P.G.L.) and 50 serum negative patients who had been living in a closed community for at least 2 years. The resul
Kuhlman JE; Deutsch JH; Fishman EK; Siegelman SS; Russell H. Morgan Department of Radiology and Radiological; Science, Johns Hopkins Medical Institutions, Baltimore, MD 21205.
The authors review the computed tomographic (CT) features of thoracic tuberculosis and other mycobacterial infections throughout their progressive stages. The spectrum of parenchymal findings seen in mycobacterial disease as well as the chronic changes of prior tuberculosis are illustrated. Altered appearances of tube
De Clercq E; Faculty of Medicine, Katholieke Universiteit Leuven, Belgium.
Eradication of human immunodeficiency virus (HIV) from infected cells or organisms has remained an elusive goal. In principle, any of the steps in the replicative cycle could form the basis for rational design of chemotherapeutic agents, although in practice not all have so far proved amenable to intervention. Here, E
Am J Psychiatry. 1990 Jun;147(6):696-710. Unique Identifier : AIDSLINE MED/90261766
Perry SW; Department of Psychiatry, Cornell University Medical College-The; New York Hospital, NY 10021.
HIV directly affects the CNS, primarily causing subcortical neuropathology. Dementia as the initial presentation is rare, but organic mental changes that mimic many functional disorders can occur during the course of infection. The mental status examination is not adequately sensitive to detect noncognitive dysfunctio
Ann Dermatol Venereol. 1990;117(2):117-21. Unique Identifier : AIDSLINE MED/90262076
Caumes E; Baspeyras M; Touraine R; Roujeau JC; Service de Dermatologie, Hopital Henri-Mondor, Universite Paris; XII, Creteil.
On the occasion of the Journees Dermatologiques de Paris, in March 1988 we designed a survey to evaluate the sterile practices of French dermatologists in the AIDS era. During the two days meeting, attended by 2,584 participants, 472 questionnaire forms were filled in (answer rate 18.5 p. 100). Among responders 43 p.
Bethea M; Forman DT; Division of Clinical Chemistry, North Carolina Memorial Hospital,; University of North Carolina, Chapel Hill 27599.
beta 2-Microglobulin (beta 2M), an interesting and underutilized metabolite, can be used in assessing renal function, particularly in kidney-transplant recipients and in patients suspected of having renal tubulointerstitial disease. It also can serve as a nonspecific but relatively sensitive marker of various neoplast
Biemer JJ; St. Joseph's Hospital, Tampa, FL 33607.
An increased incidence of malignant lymphomas is common to all types of immunodeficient patients whether they be of the natural or constitutionally occurring type, acquired as in acquired immunodeficiency syndrome (AIDS) or of iatrogenic origin as in organ transplantation. Although there is some degree of heterogeneit
Schweiberer L; Stutzle H; Mandelkow HK; Chirurgische Klinik Innenstadt der Universitat Munchen, Federal; Republic of Germany.
According to one principle of surgery, the transplantation of vital tissue is the best method of reconstructing a defect. Because of absent immunologic reactions, high osteogenic potency, and preserved stability, transplantation of autogenous bone shows the best results. Necrosis of transplanted bone, leading inevitab
AIDS Res Hum Retroviruses. 1990 Feb;6(2):243-9. Unique Identifier : AIDSLINE MED/90226893
Bernard J; Reveil B; Najman I; Liautaud-Roger F; Fouchard M; Picard O; Cattan A; Mabondzo A; Laverne S; Gallo RC; et al; Institut Jean-Godinot, Reims, France.
Most attempts to produce a vaccine against HIV-1 infection are utilizing envelope protein components. Hypothetically such vaccine candidates could stimulate production of antibodies that enhance HIV-1 infection via the macrophage route of entry and, consequently, cannot be detected in the conventional neutralization a
Akutsu Y; Mori K; Suzuki S; Ishikawa M; Sakai H; Third Department of Internal Medicine, Tohoku University School; of Medicine, Sendai.
Intravenous administration of a high dose of intact-immunoglobulin to a human immunodeficiency virus (HIV) infected patient with hemophilia A, whose total lymphocyte and CD4 positive lymphocyte counts showed a decreasing tendency, resulted in a gradual rise in these cell counts. The results were quite reproducible on
Venkatesh LK; Mohammed S; Chinnadurai G; Institute for Molecular Virology, St. Louis University School of; Medicine, Missouri 63110.
The rev gene of human immunodeficiency virus type 1 (HIV-1) encodes a 116 amino acid nuclear regulatory protein (Rev) that increases the cytoplasmic expression of viral mRNAs containing the Rev response element (RRE) and coding for the structural proteins, Gag and Env. To identify the functional domains of Rev, amino
Shirazian D; Herzlich BC; Mokhtarian F; Grob D; Department of Medicine, Maimonides Medical Center, Brooklyn, NY; 11219.
There is a significant rate of percutaneous injury with needles during the care of patients with acquired immunodeficiency syndrome (AIDS). Following puncture injury, it is recommended that the source of the contaminating blood be checked, and if human immunodeficiency virus-type 1- (HIV-1)-seropositive, zidovudine pr
Science. 1990 May 4;248(4955):588-91. Unique Identifier : AIDSLINE GENBANK/M33672
Wright JJ; Gunter KC; Mitsuya H; Irving SG; Kelly K; Siebenlist U; Laboratory of Immunoregulation, National Institute of Allergy and; Infectious Diseases, National Institutes of Health, Bethesda, MD; 20892.
Gene products encoded by the human T cell leukemia virus (HTLV) types I and II mediate transformation by the transactivation of cellular genes necessary for proliferation, probably including transcriptional regulatory factors. By searching for factors that may control proliferation, a zinc finger gene (225) was identi
FEBS Lett. 1990 Apr 24;263(2):217-21. Unique Identifier : AIDSLINE MED/90242976
Aepinus C; Voll R; Broker M; Fleckenstein B; Institut fur Klinische und Molekulare Virologie, Universitat; Erlangen-Nurnberg, FRG.
The rev protein of human immunodeficiency virus type 1 (HIV-1), a phosphoprotein of 20 kDa apparent molecular mass, is essential to target the mRNA for virion polypeptides into the cytoplasm. This effect is mediated by a specific RNA stretch (rev-responsive element = RRE) localized within a 3 -terminal segment of the
Mondino BJ; Sidikaro Y; Mayer FJ; Sumner HL; Uveitis Center, Jules Stein Eye Institute, University of; California-Los Angeles School of Medicine 90024-1771.
We measured levels of protein, of complement-derived anaphylatoxins (C3a, C4a, and C5a), and of the lymphokines interleukin-2 and gamma interferon, in vitreous humor from 10 AIDS patients with vitritis and retinitis (group 1). We compared these measurements with levels in vitreous from 7 patients with vitritis but wit
Rocancourt D; Bonnerot C; Jouin H; Emerman M; Nicolas JF; Unite de Biologie moleculaire du Developpement, Unite; Associee 1148 du Centre National de la Recherche Scientifique,; Institut Pasteur, Paris, France.
A quantitative bioassay for human immunodeficiency viruses has been developed on the basis of the ability of the tat gene to transactivate the expression of an integrated beta-galactosidase gene in a HeLa-CD4+ cell line. Infection by a single virion of HIV-1 or HIV-2 corresponds to a unique blue syncytium or a cell cl
Dormont D; Laboratoire de Neuropathologie Experimentale et Neurovirologie,; Centre de Recherches, Fontenay-Aux-Roses, France.
Neither vaccine nor therapy are, to date, available against human HIV infections. Because few is known on human pathogenesis, a standardized animal model is urgently required. Today, different models have been available: 1) partial models of the human infection (murine retrovirus, infection of SCID or transgenic mice
Eiseman B; Schrotter G; Health Sciences Center, University of Colorado, Denver.
The surgeon has to keep abreast of changing advantages and disadvantages as well as alternatives in the context of homologous blood transfusions. We ought to realise that normovolaemic anaemia is better tolerated than we used to assume in the past. It is no longer necessary to provide transfusions to patients with 10
Martino GV; Grimaldi LM; Moiola L; Castagna A; Novati R; D'Armini-Monforte A; Massironi E; Marchisio P; Tornaghi R; Principi N; et al; Universita di Milano, Clinica Neurologica IV, IRCSS Ospedale,; San Raffaele.
We have performed an isoelectric focusing study (IEF) to detect the presence of oligoclonal bands (OB) in serum and in 5 of the correspondent cerebrospinal fluid (CSF) from 14 HIV-seropositive mothers and their newborns. CB were also searched in serum from 4 newborns every 3 months over a period of 12 months. OB were
Heagy W; Groopman J; Schindler J; Finberg R; Dana-Farber Cancer Institute, Laboratory of Infectious Diseases,; Boston, MA 02115.
The tolerance and toxicity of interferon-gamma (IFN-gamma) was assessed in a phase I/II study of 21 patients with acquired immune deficiency syndrome (AIDS). A highly purified preparation of human recombinant E. coli-produced IFN-gamma was given i.v. twice weekly for an 8 week period. Patients were enrolled in the stu
Proc Natl Acad Sci U S A. 1990 May;87(10):3972-6. Unique Identifier : AIDSLINE MED/90251679
Jehuda-Cohen T; Slade BA; Powell JD; Villinger F; De B; Folks TM; McClure HM; Sell KW; Ahmed-Ansari A; Department of Pathology, Emory University School of Medicine,; Atlanta, GA 30322.
Identification of human immunodeficiency virus type 1 (HIV-1)-infected individuals is of paramount importance for the control of the spread of AIDS worldwide. Currently, the vast majority of screening centers throughout the world rely on serological techniques. As such, clinically asymptomatic but HIV-infected, serone
Meignan M; Guillon JM; Denis M; Joly P; Rosso J; Carette MF; Baud L; Parquin F; Plata F; Debre P; et al; Service de Medecine Nucleaire, Hopital Henri Mondor, Creteil,; France.
HIV-related lymphocytic alveolitis is common in HIV-seropositive patients without lung infection or tumor. In some of them a fraction of alveolar lymphocytes are HIV-specific cytotoxic T-lymphocytes (CTL) bearing the CD8 and D44 cell surface markers and capable of killing HIV-infected alveolar macrophages. In order to
Ann Intern Med. 1990 Jun 15;112(12):942-8. Unique Identifier : AIDSLINE MED/90253109
Johanson JF; Sonnenberg A; Veterans Affairs Medical Center, Milwaukee, Wisconsin.
STUDY OBJECTIVE: To compare the efficacy and cost effectiveness of alternative strategies for the evaluation and medical management of diarrhea in patients with the acquired immunodeficiency syndrome (AIDS). DESIGN: Medical decision analysis using a decision tree. METHODS: Three management strategies were compared: fu
AIDS Res Hum Retroviruses. 1990 Mar;6(3):393-9. Unique Identifier : AIDSLINE MED/90253926
Masihi KN; Lange W; Rohde-Schulz B; Chedid L; Robert Koch Institute, Federal Health Office, West Berlin,; Germany.
In the search for compounds capable of inducing endogenous production of colony-stimulating factor (CSF) and possessing activity against human immunodeficiency virus (HIV), an immunomodulator, muramyl dipeptide (MDP), was investigated. MDP can enhance monocyte-macrophage CSF in serum and promote nonspecific resistance
AIDS Res Hum Retroviruses. 1990 Apr;6(4):455-63. Unique Identifier : AIDSLINE MED/90253934
Croxson TS; Mathur-Wagh U; Handwerger S; Siroty WE; Kaminisky D; Reagan KJ; Yancovitz SR; Mildvan D; Department of Medicine, Beth Israel Medical Center, New York, NY.
Human immunodeficiency virus, type 1 (HIV-1), produces a chronic infection with a long latency before clinical disease. We followed 214 untreated subjects for 12-42 months to study the natural history of HIV infection: 110 were classified as asymptomatic, 11 as AIDS-related complex (ARC), 15 as AIDS with Kaposi s sarc
AIDS Res Hum Retroviruses. 1990 Apr;6(4):515-24. Unique Identifier : AIDSLINE MED/90253939
Holzer TJ; Allen RG; Heynen CA; Kennedy MM; Knigge MF; Paul DA; Dawson GJ; Abbott Laboratories, Experimental Biology Research, North; Chicago, IL 60064.
Serum and plasma samples were collected from blood donors who were confirmed positive for antibodies to HIV-1 in the United States , and from blood donors and individuals in West Africa and Portugal w
Int J Dermatol. 1990 Jan-Feb;29(1):24-30. Unique Identifier : AIDSLINE MED/90229171
Lim W; Sadick N; Gupta A; Kaplan M; Pahwa S; Department of Pediatrics, North Shore University Hospital,; Manhasset, NY 11030.
Since the recognition of the acquired immunodeficiency syndrome (AIDS) in 1981, several dermatologic manifestations have been associated with the syndrome, including candidiasis, dermatophyte infection, molluscum contagiosum, herpes simplex and zoster, bacterial infection, and malignancy. Skin diseases have been obser
Ikewaki N; Tamauchi H; Yamaura N; Takahashi H; Inoko H; Department of Microbiology, School of Nursing, Kitasato; University, Kanagawa.
A cell line secreting a human monoclonal antibody was established by Epstein-Barr virus transforming B cells derived from an enlarged cervical lymph node excised from a patient bearing a carotid body tumor. The reactivity of the monoclonal antibody, designated as mNISP, was tested on various cells and cell lines. An a
Shellito J; Suzara VV; Blumenfeld W; Beck JM; Steger HJ; Ermak TH; Section of Respiratory Care, Veterans Administration AIDS; Research Center, San Francisco, California.
Pulmonary infections with Pneumocystis carinii are an important cause of morbidity and mortality in patients with AIDS. P. carinii infections are seen in patients with decreased numbers of helper T lymphocytes, suggesting that these cells are important in preventing infection. To test this hypothesis, we sought to est
J Immunol. 1990 May 15;144(10):3928-35. Unique Identifier : AIDSLINE MED/90237509
Ruegg CL; Strand M; Department of Pharmacology and Molecular Sciences, Johns Hopkins; University School of Medicine, Baltimore, MD 21205.
We have previously shown that a synthetic peptide containing env residues 581-597 from HIV-1 inhibits lymphoproliferation of human PBMC. We have investigated the molecular mechanism(s) by which this HIV-1-derived peptide inhibits CD3-mediated signal transduction. We show that the peptide containing residues 581-597 fr
West J Med. 1990 Mar;152(3):261-7. Unique Identifier : AIDSLINE MED/90239929
Dixon PS; Bodner AJ; Okihiro M; Milbourne A; Diwan A; Nakamura JM; Department of Medicine, Miriam Hospital, Providence, Rhode; Island.
Tropical spastic paraparesis or human T-lymphotropic virus type I (HTLV-I)-associated myelopathy is a degenerative encephalomyelopathy with pyramidal tract dysfunction affecting the lower extremities. It is associated with HTLV-I infection and found primarily in the Caribbean region and in southwestern
Bruckova M; Kopecka D; Syrucek L; Institut hygieny a epidemiologie, Praha.
The author describes and evaluates different approaches in laboratory diagnosis of HIV infection and AIDS. Serological detection of anti-HIV antibodies provides despite some limitations very good diagnostic opportunities. ELISA screening tests supplemented by confirming tests, in particular the Western blot, ensure ma
Cohen EA; Dehni G; Sodroski JG; Haseltine WA; Division of Human Retrovirology, Dana-Farber Cancer Institute,; Boston, Massachusetts.
The vpr product of the human immunodeficiency virus type 1 (HIV-1) acts in trans to accelerate virus replication and cytopathic effect in T cells. Here it is shown that the HIV-1 viral particle contains multiple copies of the vpr protein. The vpr product is the first regulatory protein of HIV-1 to be found in the viru
Klin Wochenschr. 1990 Mar 16;68(6):299-305. Unique Identifier : AIDSLINE MED/90244582
Krapf FE; Herrmann M; Leitmann W; Schwartlander B; Kalden JR; Institut fur Klinische Immunologie und Rheumatologie,; Universitat Erlangen-Nurnberg.
Circulating immune complexes are part of normal immune defense mechanisms and, therefore, present in various infectious--bacterial and viral--diseases. On the other hand, they are obviously involved in pathogenic mechanisms, e.g., autoimmune diseases or different forms of malignancies. Both autoimmune and infectious f
Schellekens PT; Roos MT; De Wolf F; Lange JM; Miedema F; Central Laboratory, The Netherlands Red Cross Blood Transfusion; Service, University of Amsterdam.
In a cohort of 300 HIV-1-infected homosexual men, studied longitudinally, the prognostic value of T-cell proliferative responses for development of AIDS was analyzed. In 15 persons we observed that, at seroconversion, T-cell reactivity to anti-CD3 monoclonal antibody (Mab) and phytohemagglutinin (PHA) was decreased to
Till MA; Ghetie V; May RD; Auerbach PC; Zolla-Pazner S; Gorny MK; Gregory T; Uhr JW; Vitetta ES; Department of Microbiology, University of Texas Southwestern; Medical Center, Dallas 75235.
We have previously reported that immunoconjugates composed of deglycosylated ricin A chain coupled to either recombinant (r) CD4 or two different monoclonal human anti-gp41 antibodies (rCD4-dgA and anti-gp41-dgA, respectively) are specifically toxic to HIV-infected lines of human T cells (H9) and monocytes (U937). In
Rio B; Louvet C; Gessain A; Dormont D; Gisselbrecht C; Martoia R; Auzanneau G; Miclea JM; Baumelou E; Dombret H; et al; Service d'hematologie, Hotel Dieu, Paris.
Adult T-cell leukaemia is the first blood disease caused by a retrovirus: HTLV-1. The authors report the first French series of 15 patients, of whom 9 came from the classical endemic areas--the Antilles and outer Caribbean Islands--and 6 from Africa where the serological prevalence of HTLV-1 is high but few cases of a
Laulan S; Bernard JF; Boivin P; Service d'Hematologie Clinique, Hopital Beaujon, Clichy.
Eleven patients (5 men and 6 women) with sickle-cell anaemia were treated with systematic repeated blood transfusions to avoid the sickling crises and complications of the disease and were followed up for 5 to 10 years. On average, the patients received 22 erythrocyte concentrates per year, to a mean individual total
Peripheral T-cell lymphomas (PTCL) represent a new subset of malignant lymphomas, which demonstrates a marked morphological, immunological and clinical diversity. They seem to have a worse prognosis globally than B-cell lymphomas. The main clinical characteristics and outcome of PTCL are analysed in this series of 22
A review of the spectrum of dermatologic findings related to human immunodeficiency virus infection is presented. Oral candidiasis, seborrheic dermatitis, oral hairy leukoplakia, herpes simplex infections and molluscum contagiosum were among the most frequently encountered dermatologic disorders among the HIV-seroposi
Alter MJ; Favero MS; Moyer LA; Miller JK; Bland LA; Centers for Disease Control, Atlanta, GA 30333.
To determine trends in a variety of dialysis-associated diseases and practices, the Centers for Disease Control surveyed 1,734 chronic hemodialysis centers in the United States in 1988 in conjunction with the annual facility survey performed by the Health Care Financing Administration.
Davey RT Jr; Masur H; Laboratory of Immunoregulation, National Institute of Allergy and; Infectious Diseases, National Institutes of Health, Bethesda,; Maryland 20892.
In summary, recent advances in our ability to diagnose, treat, and prevent recurrences of pneumocystis pneumonia have significantly improved the clinical management of this infection, especially in HIV-1-infected individuals. As current investigations allow our therapeutic armamentarium in this disease to be strengthe
Blood and body fluids and Universal Precautions have become part of the everyday vocabulary in health care. How body fluids are handled by individuals depends on their perception of risk for acquiring an infection from these substances and is not always based on sound knowledge. An understanding of how infections are
AIDS Res Hum Retroviruses. 1990 Feb;6(2):229-41. Unique Identifier : AIDSLINE MED/90226892
Mawle AC; Thieme ML; Ridgeway MR; McDougal JS; Schmid DS; Immunology Branch, Centers for Disease Control, U.S. Public; Health Service, Atlanta, GA.
Previously, we demonstrated that cultures of human peripheral blood lymphocytes (PBL) stimulated with herpes simplex virus type 1 (HSV-1) generate antigen-specific, major histocompatibility complex (MHC) restricted cytotoxic T lymphocytes (CTL) that tend to be CD4+ and restricted to HLA-DR antigens. In this study, we
AIDS Res Hum Retroviruses. 1990 Feb;6(2):261-9. Unique Identifier : AIDSLINE MED/90226895
Rossi G; Gorla R; Stellini R; Franceschini F; Bettinzioli M; Cadeo G; Sueri L; Cattaneo R; Marinone G; Sezione di Ematologia, Universita di Brescia, Spedali Civili,; Italy.
Prevalence and clinical features of human immunodeficiency virus (HIV)-related thrombocytopenia have been investigated among a random population of 657 anti-HIV-positive individuals. A platelet count below 100 X 10(9)/liter was detected in 72 patients (10.9%). Compared with anti-HIV-positive controls with normal plate
Can Med Assoc J. 1990 May 1;142(9):937-46. Unique Identifier : AIDSLINE MED/90227559
Stock SR; Gafni A; Bloch RF; Community Medicine Residency Program, McMaster University,; Hamilton, Ont.
The universal precautions recommended by the US Centers for Disease Control (CDC), Atlanta, for the prevention of HIV (human immunodeficiency virus) transmission to health care workers are widely accepted, despite little documentation of their effectiveness and efficiency. We reviewed the evidence on the risk of HIV t
J Am Vet Med Assoc. 1990 Apr 1;196(7):1049-52. Unique Identifier : AIDSLINE MED/90229326
Swenson CL; Kociba GJ; Mathes LE; Hand PJ; Neer CA; Hayes KA; Olsen RG; Department of Veterinary Pathobiology, College of Veterinary; Medicine, Ohio State University, Columbus 43210.
Feline leukemia virus status and antibody titer to feline oncornavirus-associated cell membrane antigen (FOCMA) were determined on plasma from 183 outpatient cats and 61 cats from 2 closed, FeLV-positive, multiple-cat households. Cats with FOCMA antibody titer had a significantly (P less than 0.02) higher prevalence o
J Med Chem. 1990 May;33(5):1353-60. Unique Identifier : AIDSLINE MED/90230236
Beres J; Sagi G; Tomoskozi I; Gruber L; Baitz-Gacs E; Otvos L; De Clercq E; Central Research Institute for Chemistry, Hungarian Academy of; Sciences, Budapest.
Enantiomerically pure (+)- and (-)-carbocyclic thymidine, (-)-carbocyclic 3 -epi-thymidine, (+)-carbocyclic 3 -deoxy-3 -azidothymidine, (+)-carbocyclic 2,3 -O-anhydrothymidine, (+)-carbocyclic 3 -O,6 -methylenethymidine, and (+)-(6 S)-carbocyclic 6 -methylthymidine were synthesized in a stereospecific manner from comm
Med Clin (Barc). 1990 Jan 20;94(2):49-52. Unique Identifier : AIDSLINE MED/90230868
de Luis A; Montalban J; Fernandez JM; Ocana I; Codina A; Martinez-Vazquez JM; Servicio de Medicina Interna-Patologia Infecciosa, Hospital; General Vall d'Hebron, Universitat Autonoma, Barcelona.
We report five patients with human immunodeficiency virus (HIV) infection in stages IV-C1, IV-C2 and IV-D who developed peripheral polyneuropathy (PNP). Two patients had invalidating PNP with a demyelinating predominance, while in the remaining three axonal component predominated. However, both components were present
Krilov LR; Rubin LG; Frogel M; Gloster E; Ni K; Kaplan M; Lipson SM; Department of Pediatrics Schneider Children's Hospital, Long; Island Jewish Medical Center, New Hyde Park, New York.
Disseminated adenovirus infection with fatal hepatic necrosis has been reported in 16 patients, 15 of whom had immunocompromising conditions. Herein we report three patients with AIDS and fatally disseminated adenovirus infection with hepatic necrosis. The median age of these 16 patients was 4.7 years, and their illne
Baier M; Garber C; Muller C; Cichutek K; Kurth R; Paul-Ehrlich-Institute, Langen, Federal Republic of Germany.
We have determined the entire nucleotide sequence of a full-length molecular clone, termed SIVagm3, which is infectious in vitro and in vivo. The genomic organization was found to be similar to other immunodeficiency viruses of human and simian origin. Comparison of SIVagm3 with SIVagmTYO-1, the only other completely
Rando RF; Srinivasan A; Feingold J; Gonczol E; Plotkin S; Department of Pathology, Pennsylvania Hospital, Philadelphia; 19107.
In transient gene expression assays we observed an increase in expression of the bacterial chloramphenicol acetyl-transferase (CAT) gene, under the transcriptional control of the HIV-1 LTR (pLTR-CAT), when this plasmid was cotransfected into Vero or MRC-5 cells with a plasmid containing either the HCMV immediate early
No To Hattatsu. 1990 Mar;22(2):103-11. Unique Identifier : AIDSLINE MED/90234562
Matsumoto S; Sakiyama Y; Kajii N; Okano M; Ishizaka A; Ariga T; Department of Pediatrics, Hokkaido University School of Medicine,; Sapporo.
Ataxia telangiectasia (AT) is a primary immunodeficiency syndrome characterized by cerebellar ataxia, extrapyramidal signs, oculocutaneous telangiectasia, recurrent respiratory infections and development of malignancies. AT is a complex autosomal recessive disorder involving several systems other than lymphoid cells o
Fedor-Chaiken M; Deschenes RJ; Broach JR; Department of Biology, Princeton University, New Jersey 08544.
We have identified a gene, SRV2, mutations of which alleviate stress sensitivity in strains carrying an activated RAS gene. Epistasis analysis suggests that the gene affects accumulation of cAMP in the cell. Direct assays of cAMP accumulation indicate that mutations of the gene diminish the rate of in vivo production
Miles PR; Baughman RP; Linnemann CC Jr; Department of Internal Medicine, University of Cincinnati Medical; Center.
This study investigated the significance of detecting cytomegalovirus in the bronchoalveolar lavage fluid of patients with human immunodeficiency virus infection. Bronchoscopy with BAL was performed on all patients. Lavage was examined for
Gene. 1990 Mar 15;87(2):243-8. Unique Identifier : AIDSLINE GENBANK/M36450
Cheng YS; McGowan MH; Kettner CA; Schloss JV; Erickson-Viitanen S; Yin FH; Central Research and Development Department, E.I. duPont de; Nemours and Co., Wilmington, DE 19880-0328.
A complete chemical synthesis and assembly of genes for the production of human immunodeficiency virus type-I protease (HIV-PR) and its precursors are described. The T7 expression system was used to produce high levels of active HIV-PR and its precursors in Escherichia coli inclusion bodies. The gene encoding the open
Tremblay M; Meloche S; Sekaly RP; Wainberg MA; Lady Davis Institute, Jewish General Hospital, Montreal, Quebec,; Canada.
Although the CD4 glycoprotein is the primary receptor for HIV-1, recent reports have suggested that other molecules might be involved in the enhancement of HIV-1 infection. We investigated the possible role of the complement receptor 2 in enhancement of HIV-1 infection in CD4+ EBV-containing B cells by infecting such
Ringler DJ; Walsh DG; Chalifoux LV; MacKey JJ; Daniel MD; Desrosiers RC; King NW; Hancock WW; Harvard Medical School, New England Regional Primate Research; Center, Southborough, Massachusetts.
More than 80% of rhesus monkeys infected with simian immunodeficiency virus (SIV) were found to have elevated levels of soluble interleukin-2 receptor (IL-2R) in their serum during the course of infection. All long-term survivors had stably elevated levels of soluble IL-2R. The highest levels of soluble IL-2R correlat
Proc Natl Acad Sci U S A. 1990 May;87(9):3479-83. Unique Identifier : AIDSLINE MED/90239037
Tada H; Rappaport J; Lashgari M; Amini S; Wong-Staal F; Khalili K; Department of Biochemistry and Molecular Biology, Jefferson; Institute of Molecular Medicine, Jefferson Medical College,; Thomas Jefferson University, Philadelphia, PA 19107.
Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central nervous system caused by the JC virus (JCV), a human papovavirus. PML is a relatively rare disease seen predominantly in immunocompromised individuals and is a frequent complication observed in AIDS patients. The significantly h
Horn TD; Hood AF; Department of Dermatology, Johns Hopkins University School of; Medicine, Baltimore, Md.
We examined five consecutive skin biopsy specimens taken from perineal ulcers on immunosuppressed patients. Examination of hematoxylin-eosin-stained sections in conjunction with immunohistochemistry using monoclonal antibodies to early and late viral antigens resulted in identification of
Int J Cancer. 1990 May 15;45(5):883-8. Unique Identifier : AIDSLINE MED/90243374
Boiocchi M; Carbone A; De Re V; Dolcetti R; Volpe R; Tirelli U; Division of Experimental Oncology I, Centro di Riferimento; Oncologico, Aviano, Italy.
Six AIDS-related NHLs from direct blood-stream HIV-infected patients were characterized for clonality, maturation cell characteristics, activation of c-myc proto-oncogene and presence of HIV and EBV genomes. Four out of the 6 AIDS-related NHLs were of immature B-cell origin, contrasting with the lower frequency (2 out
Vacca A; Martinotti S; Screpanti I; Maroder M; Felli MP; Farina AR; Gismondi A; Santoni A; Frati L; Gulino A; Department of Experimental Medicine, University La Sapienza,; Rome, Italy.
Dexamethasone treatment of the Jurkat T77 cell clone inhibited the enhancing effect of 12-O-tetradecanoylporbol-13-acetate (TPA) and the calcium ionophore A23187 on the interleukin 2 (IL2) mRNA levels and gene transcription from intact nuclei. Dexamethasone treatment of Jurkat T77 cells inhibited the TPA/A23187-depend
Cao YZ; Friedman-Kien AE; Huang YX; Li XL; Mirabile M; Moudgil T; Zucker-Franklin D; Ho DD; Department of Microbiology, New York University Medical Center,; New York 10016.
Five hepatoma cell lines, including CZHC/8571, PLC/PRF/5, Hep3B, HepG2, and HUH7, were inoculated with three diverse isolates of human immunodeficiency virus type 1 (HIV-1). Productive infection was noted in all hepatoma cell lines, and expression of viral p24 antigen lasted for over 3 months, but its level decreased
Watanabe N; Nishi M; Ikawa Y; Amanuma H; Laboratory of Gene Technology and Safety, Institute of Physical; and Chemical Research (RIKEN), Ibaraki, Japan.
To determine the biological significance of the 585-base-pair deletion in the env gene of Friend spleen focus-forming virus (SFFV) encoding a leukemogenic glycoprotein (gp55), we examined the pathogenicity of a constructed mutant SFFV (SFFVDF). In the SFFVDF genome, the env deletion was filled in with the correspondin
Lusso P; Markham PD; DeRocco SE; Gallo RC; Laboratory of Tumor Cell Biology, National Cancer Institute,; National Institutes of Health, Bethesda, Maryland 20892.
The in vitro susceptibility of several nonhuman primate species to human herpesvirus 6 (HHV-6) was investigated. Only peripheral blood mononuclear cells from chimpanzees (Pan troglodytes) were found permissive to productive infection by HHV-6, indicating that the host range of HHV-6, albeit limited, may not be restric
Barry PA; Pratt-Lowe E; Peterlin BM; Luciw PA; Department of Medical Pathology, University of California, Davis; 95616.
Proteins encoded by a variety of DNA viruses activate gene expression from the promoter within the long terminal repeat (LTR) of the human immunodeficiency virus type 1 (HIV-1). The mechanism by which immediate-early (IE) gene products of human cytomegalovirus (
Hirschel B; Departement de medecine interne, Hopital cantonal; universitaire, Geneve.
The majority of patients with Aids suffer from diarrhea and weight loss, as well as opportunistic infection and tumors of the gastrointestinal tract; endoscopy is frequently necessary. Often, but not always, it is possible to identify an opportunistic tumor or infection which explains the patient s signs and symptoms.
Ozel M; Pauli G; Gelderblom HR; Robert Koch-Institut des Bundesgesundheitsamtes, Berlin, Germany.
Electron spectroscopic imaging (ESI) and conventional bright-field transmission electron microscopy (TEM) were applied comparatively for the analysis of the fine structure and the antigenic make-up of human immunodeficiency virus and two herpes viruses. In addition to the information obtained in conventional bright-fi
Tomita T; Chiga M; Lenahan M; Balachandran N; Department of Pathology, University of Kansas Medical Center,; Kansas City 66103.
Cytomegalovirus ( CMV ) infection was observed in 10 of 12 autopsy cases of acquired immunodeficiency syndrome (AIDS) and appears to be the commonest life-threatening viral infection in AIDS. In all 10 cases, adrenal glands were affected with CMV and adrenal medul
Martino GV; Grimaldi LM; Moiola L; Cichero P; Ieri R; Bonini PA; Clinica Neurologica IV, Universita di Milano.
We have measured the levels of antibody (ab) against different Epstein-Barr virus (EBV) antigens in 10 AIDS patients, 7 HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients and 20 control subjects. We found comparable serum levels of anti-EBV ab between AIDS patients, HAM/TSP patients and contr
Ferrari S; Bonetti B; Monaco S; Cavallaro T; Polo A; Di Perri G; Danzi MC; Malena M; Luzzati R; Cruciani M; et al; Istituto di Neurologia, Universita di Verona.
Three patients with predominantly sensory neuropathy in course of HIV-1 infection are reported. In all cases ultrastructural examination showed herpes virus-like particles in the axoplasmic matrix of myelinated fibers and in endoneurial macrophages. In 2 cases clinical patterns of posterior column involvement were obs
Am J Med. 1990 May 21;88(5B):11S-15S. Unique Identifier : AIDSLINE MED/90247431
Merigan TC; Skowron G; Division of Infectious Diseases, Stanford University School of; Medicine, California 94305.
Phase I and II clinical studies have been conducted to test the safety and potential activity of the reverse transcriptase inhibitor, dideoxycytidine ( ddC ), in treating human immunodeficiency virus-1-infected patients. Although ddC appears to be activ
Am J Med. 1990 May 21;88(5B):16S-19S. Unique Identifier : AIDSLINE MED/90247432
Pizzo PA; Pediatric Branch, National Cancer Institute, Bethesda, Maryland; 20892.
The safety and activity of several antiretroviral agents are being evaluated for treatment of acquired immunodeficiency syndrome (AIDS) in infants and children. Intermittent oral and intravenous regimens and continuous intravenous infusion of the dideoxynucleoside, 3 -azido-3 -deoxythymidine (zidovudine,
Am J Med. 1990 May 21;88(5B):20S-23S. Unique Identifier : AIDSLINE MED/90247434
Skowron G; Merigan TC; Division of Infectious Disease, Roger Williams General Hospital,; Providence, Rhode Island 02908.
The deoxynucleoside analogues 2 ,3 -dideoxy-cytidine ( ddC ) and 3 -azido-3 -deoxythymidine (zidovudine, AZT ) are active as single agents in conferring immunologic and virologic benefit
Am J Med. 1990 May 21;88(5B):24S-26S. Unique Identifier : AIDSLINE MED/90247435
Bozzette SA; Richman DD; Division of Infectious Diseases, University of California, San; Diego.
Zidovudine ( AZT ) prolongs life in patients with acquired immunodeficiency syndrome (AIDS) or AIDS-related complex but often causes dose-limiting bone marrow suppression in this population. This has prompted a search for salvage therapies for use in pe
Am J Med. 1990 May 21;88(5B):27S-30S. Unique Identifier : AIDSLINE MED/90247436
Meng TC; Fischl MA; Richman DD; University of California San Diego Treatment Center 92103.
The dideoxynucleosides 3 -azido-3 -deoxythymidine (zidovudine, AZT ) and 2 ,3 -dideoxycytidine ( ddC ) are potent inhibitors of human immunodeficiency virus (HIV) in vitro and improve su
Am J Med. 1990 May 21;88(5B):31S-33S. Unique Identifier : AIDSLINE MED/90247437
Broder S; Yarchoan R; National Cancer Institute, National Institutes of Health,; Bethesda, Maryland 20892.
The reverse transcriptase inhibitor 2 ,3 -dideoxycytidine ( ddC ) is capable of mediating virologic and immunologic improvements in some patients with acquired immunodeficiency syndrome (AIDS) or AIDS-related complex. However, severe
Rouah E; Rogers BB; Wilson DR; Kirkpatrick JB; Buffone GJ; Department of Pathology, Baylor College of Medicine, Houston, TX.
Primary lymphomas of the central nervous system (CNS) account for 0.3% to 1.5% of all intracranial neoplasms. Several reports have noted a coincidence between this neoplasm and serologic evidence of Epstein-Barr virus (EBV) infection, but in only a few instances has the EBV genome been demonstrated in these tumors. To
Albert S; Kuhnl P; Sibrowski W; Seidl S; Doerr HW; Zentrum der Hygiene, Universitats-Klinik Frankfurt/Main.
The subjects of the present study are diagnostic and epidemiologic aspects in the determination of serum antibodies to cytomegalovirus in blood donors, organ donors, dialysis patients and drug addicts (some of whom were HIV-positive); antibodies were determined using the ELA-Test (
Greenspan D; De Souza YG; Conant MA; Hollander H; Chapman SK; Lennette ET; Petersen V; Greenspan JS; Oral AIDS Center, University of California, San Francisco; 94143-0512.
The efficacy of desciclovir, an analog of acyclovir , in eliminating lesions of oral hairy leukoplakia (HL) and suppressing Epstein-Barr virus (EBV) infection was evaluated in a double-blind, placebo-controlled study of 14 patients. Patients were randomized to receive either the
Jessurun J; Angeles-Angeles A; Gasman N; Unidad de Patologia, Hospital General de Mexico SS, Departamento; de Patologia, Facultad de Medicina, UNAM.
In order to identify the characteristics of the acquired immune deficiency syndrome (AIDS) as it occurs in Mexico , a comparative study of the demographic and pathological findings of the first 58 patients who died of AIDS and were autopsied at two Mexican hospitals, Hospital Genera
Ruprecht RM; Chou TC; Chipty F; Sosa MG; Mullaney S; O'Brien L; Rosas D; Department of Medicine, Harvard Medical School, Boston,; Massachusetts.
This study was undertaken to calculate the in vivo drug interactions between recombinant human interferon-alpha A/D (rHuIFN-alpha A/D) and 3 -azido-3 -deoxythymidine ( AZT ) in a quantitative model for retroviral viremia. When given as single agents, bo
Sera from 353 cats with naturally occurring feline leukemia virus (FeLV) infection were collected in Boston, Los Angeles, New York City, and Seattle between 1968 and 1988. These sera were retrospectively assayed by enzyme-linked immunosorbent assay for antibodies to feline immunodeficiency virus (FIV). Fifty-one (14.4
J Med Primatol. 1990;19(1):9-20. Unique Identifier : AIDSLINE MED/90250754
Honjo S; Narita T; Kobayashi R; Hiyaoka A; Fujimoto K; Takasaka M; Sakakibara I; Mukai R; Ishikawa K; Ohta Y; et al; Tsukuba Primate Center for Medical Science, National Institute of; Health, Tsukuba-shi, Japan.
An infection occurred in all African green monkeys and cynomolgus monkeys experimentally inoculated with SIVAGM [TYO-1], as demonstrated by the appearance of an antibody to SIVAGM [TYO-1] and the isolation of the virus. No monkey exhibited overt clinical disorders throughout the experimental period of 42 weeks. Thus,
J Med Primatol. 1990;19(2):109-18. Unique Identifier : AIDSLINE MED/90250755
King NW; Chalifoux LV; Ringler DJ; Wyand MS; Sehgal PK; Daniel MD; Letvin NL; Desrosiers RC; Blake BJ; Hunt RD; New England Regional Primate Research Center, Harvard Medical; School, Southborough, MA.
Epidemiologic and clinicopathologic data from 11 macaques with naturally acquired SIV infection--10 of which have died--were compared with those from 34 rhesus monkeys that have died of experimental SIVmac infection. Several differences, including gender affected, age at time of death, and the occurrence of certain op
JAMA. 1990 Jun 6;263(21):2911-6. Unique Identifier : AIDSLINE MED/90250826
Palefsky JM; Gonzales J; Greenblatt RM; Ahn DK; Hollander H; Department of Laboratory Medicine, University of California-San; Francisco 94143.
Ninety-seven male homosexuals with the acquired immunodeficiency syndrome or other group IV human immunodeficiency virus disease were studied for anal human papillomavirus infection and intra-anal cytological abnormalities. Human papillomavirus DNA was detected in 52 subjects (54%), and 38 subjects (39%) were found to
Rohrig S; Grob PJ; Departement fur Innere Medizin, Universitatsspital Zurich.
382 i.v. drug abusers were tested for a past or ongoing infection with the hepatitis viruses A (HAV), B (HBV), the newly discovered C (HCV) and with the Aids virus HIV. The cohort studied was representative for i.v. drug users of the Zurich street scene including occasional users, weekend users and severe drug addicts
Burnouf-Radosevich M; Burnouf T; Huart JJ; Centre Regional de Transfusion Sanguine de Lille, France.
A fibrin glue preparation has been obtained from pooled human plasma using a procedure which includes a solvent-detergent (SD) treatment to inactivate lipid-enveloped viruses. The SD treatment inactivated greater than or equal to 5.5 log10 of HIV in less than 45 min, and greater than or equal to 5 log10 and greater th
Williams MD; Cohen BJ; Beddall AC; Pasi KJ; Mortimer PP; Hill FG; Haematology Department, Children's Hospital, Birmingham, UK.
The prevalence of antibody to human parvovirus B19 was determined in 86 children with congenital bleeding disorders. Forty-seven of 53 boys (89%) receiving non-heat-treated factor VIII or prothrombin complex concentrates were anti-B19 IgG positive compared with 38% of their age-matched controls and 48% of children tre
Zh Nevropatol Psikhiatr Im S S Korsakova. 1990;90(2):3-8. Unique Identifier : AIDSLINE MED/90252515
Zavalishin IA
The review is concerned with the problems of multiple sclerosis etiology, with the new data enriching the existing concepts of the given question. Special attention is paid to an analysis of the role of retroviruses, new genetic markers, and environmental factors. Approaches to further studies into the disease are out
Krieg AM; Steinberg AD; Cellular Immunology Section, National Institute of Arthritis and; Musculoskeletal and Skin Diseases, National Institutes of Health,; Bethesda, MD 20892.
Goustin AS; Galanopoulos T; Kalyanaraman VS; Pantazis P; Center for Blood Research, Boston, Massachusetts 02115.
Several HTLV-I-infected T-cell lines express the two genes encoding platelet-derived growth factor (PDGF). Therefore, we examined the question of a possible self-stimulatory mechanism of proliferation involving PDGF in these cells. Using a nucleic acid probe and an antibody specific for the PDGF-B receptor (PDGFR-B),
Pedroso de Lima MC; Chiche BH; Debs RJ; Duzgunes N; Cancer Research Institute, University of California, San; Francisco 94143-0128.
Liposomes can be used as carriers of drugs in the treatment of viral, bacterial and protozoal infections. The potential for liposome-mediated therapy of Mycobacterium avium-intracellulare complex infections, one of the most common opportunistic infections in AIDS, is currently under study. Here, we have investigated t
Cordier G; Cozon G; Greenland T; Rocher F; Guiguen F; Guerret S; Brune J; Mornex JF; INSERM U 80, CNRS URA 1177, Universite Claude Bernard, Hopital; Edouard Herriot, Lyon, France.
Sheep infected by visna-maedi virus, a lentivirus related to the human immunodeficiency virus, develop a chronic interstitial lung disease. Since monocyte/macrophages are known to be specifically infected by visna-maedi virus, we investigated the role of macrophages in the appearance of pulmonary lesions in animals wi
Eur Respir J. 1990 Mar;3(3):282-7. Unique Identifier : AIDSLINE MED/90255571
Durand-Amat S; Zalcman G; Mazeron MC; Sarfati C; Beauvais B; Gerber F; Perol Y; Hirsch A; Service de Pneumologie, Hopital Saint-Louis, Paris, France.
During a ten month period, 117 fibreoptic bronchoscopies and bronchoalveolar lavages (BAL) were performed in human immunodeficiency virus (HIV) infected patients suspected of having opportunistic pulmonary infections. The BAL were classified into 3 groups, according to clinical manifestations related to HIV infection
Mitchell SA; Welch JM; Weston-Smith S; Nicholson F; Bradbeer CS; Department of Genito-Urinary Medicine, St Thomas' Hospital; Medical School, London.
Opportunistic infections such as Pneumocystis carinii pneumonia are well-recognised in patients with the acquired immune deficiency syndrome (AIDS). Anaemia due to a variety of causes also occurs in AIDS. Persistent infection with parvovirus (B19) causing severe anaemia has been reported in patients with leukaemia and
Connolly GM; Gazzard BG; Hawkins DA; Department of Medicine, Westminster Hospital, London.
A case of fixed drug eruption (FDE) secondary to foscarnet is reported. This drug has recently become available on a compassionate use basis for treatment of cytomegalovirus ( CMV ) infection which may cause significant disease
J Immunol. 1990 Jun 1;144(11):4347-55. Unique Identifier : AIDSLINE MED/90257356
Makino M; Morse HC 3d; Fredrickson TN; Hartley JW; Laboratory of Immunopathology, National Institute of Allergy and; Infectious Diseases, National Institutes of Health, Bethesda, MD; 20892.
Host genetic determinants of resistance or susceptibility to a retrovirus-induced immunodeficiency syndrome, termed MAIDS, were evaluated in Fv-1b mice infected with the mixture of ecotropic, MCF, and defective murine leukemia viruses designated LP-BM5 murine leukemia viruses. Genes of the MHC were shown to exert a ma
Oral ulcerations associated with disseminated cytomegalovirus ( CMV ) infection were observed in four patients with AIDS manifestations showing low CD4 counts. Virus cultures of urine and saliva samples were positive for CMV in
Gross JG; Bozzette SA; Mathews WC; Spector SA; Abramson IS; McCutchan JA; Mendez T; Munguia D; Freeman WR; Department of Ophthalmology, University of California San Diego; School of Medicine.
The authors prospectively evaluated 67 consecutive patients with the acquired immune deficiency syndrome (AIDS) and cytomegalovirus ( CMV ) retinitis during a 33-month period to assess the clinical patterns of retinal infection
Anderson DJ; Jordan MC; Department of Medicine, University of Minnesota Medical School,; Minneapolis.
Viral pulmonary infections are a major cause of morbidity and mortality in solid organ transplant recipients. The herpes viruses- cytomegalovirus , herpes simplex virus, varicella zoster virus, and Epstein-Barr virus--cause most of the viral infections in this population. Respiratory
Science. 1990 Jun 1;248(4959):1109-12. Unique Identifier : AIDSLINE GENBANK/M33262
Kestler H; Kodama T; Ringler D; Marthas M; Pedersen N; Lackner A; Regier D; Sehgal P; Daniel M; King N; et al; New England Regional Primate Research Center, Harvard Medical; School, Southborough, MA 01772.
Better understanding of the pathogenesis of acquired immunodeficiency syndrome (AIDS) would be greatly facilitated by a relevant animal model that uses molecularly cloned virus of defined sequence to induce the disease. Such a system would also be of great value for AIDS vaccine research. An infectious molecular clone
Orholm M; Nielsen TL; Nielsen JO; Lundgren JD; Department of Infectious Diseases, Hvidovre Hospital, University; of Copenhagen, Denmark.
The diagnostic value of the CD4 cell counts and the HIV p24 antigen were evaluated in a consecutive series of 105 HIV-infected patients experiencing 128 episodes of pulmonary symptoms which required bronchoscopy. One-third of patients with opportunistic infection (OI) had CD4 counts greater than 0.200 x 10(9)/l, and 6
Int J Immunopharmacol. 1990;12(2):225-33. Unique Identifier : AIDSLINE MED/90229150
Itoh W; Sugawara I; Kimura S; Tabata K; Hirata A; Kojima T; Mori S; Shimada K; Department of Pathology, University of Tokyo, Japan.
Sulfated schizophyllans of differing sulfur content were prepared and their mitogenic activities and effects on human immunodeficiency virus (HIV) were investigated in vitro. The sulfated schizophyllans were found to possess mitogenic activity. Anti-viral activity was evaluated in terms of cytopathic effect, and by th
J Immunol. 1990 May 1;144(9):3266-71. Unique Identifier : AIDSLINE MED/90229709
Clerici M; Stocks NI; Zajac RA; Boswell RN; Via CS; Shearer GM; Experimental Immunology Branch, National Cancer Institute,; National Institutes of Health, Bethesda, MD 20892.
PBL from approximately 50% of asymptomatic individuals infected with HIV have been previously demonstrated to exhibit defective in vitro Th function that is selective for influenza A virus (FLU), but not for HLA alloantigens (ALLO). In this report, we have further studied HIV+ individuals with this selective Th defect
J Immunol. 1990 May 1;144(9):3341-6. Unique Identifier : AIDSLINE MED/90229719
Callahan KM; Fort MM; Obah EA; Reinherz EL; Siliciano RF; Department of Medicine, Johns Hopkins University School of; Medicine, Baltimore, MD 21205.
The propensity of HIV-1 to undergo sequence variation, particularly in the envelope glycoprotein gp120, complicates vaccine development and may enable the virus to evade ongoing immune responses in infected individuals. We present here a molecular analysis of the effects of this variability on human T cell recognition
Lancet. 1990 May 5;335(8697):1047-50. Unique Identifier : AIDSLINE MED/90230940
Levy JA; Ferro F; Greenspan D; Lennette ET; Department of Medicine, University of California, School of; Medicine, San Francisco 94143.
Human herpesvirus-6 (HHV-6) was recovered at high frequency (greater than 85%) from the saliva of both healthy individuals and those infected with the human immunodeficiency virus (HIV). The level of isolation mirrored the high prevalence of antibodies to HHV-6 found in sera obtained from residents of diverse areas of
Chowdhury IH; Koyanagi Y; Kobayashi S; Hamamoto Y; Yoshiyama H; Yoshida T; Yamamoto N; Department of Virology and Parasitology, Yamaguchi University; School of Medicine, Japan.
Cocultivation of MOLT-4 and MOLT-4/HIVHTLV-IIIB cells with more than 0.01 ng/ml of 12-O-tetradecanoylphorbol-13-acetate (TPA) for 20 hr strikingly inhibited HIV-induced syncytia formation resulting from cell to cell infection. Interestingly, the production of HIV-specific p24 antigen in the culture fluid was significa
Am J Trop Med Hyg. 1990 Apr;42(4):374-9. Unique Identifier : AIDSLINE MED/90233455
Reeves WC; Levine PH; Cuevas M; Quiroz E; Maloney E; Saxinger WC; Gorgas Memorial Laboratory, Panama.
The human T-lymphotropic virus (HTLV) and associated diseases, adult T cell leukemia and spastic paraparesis, appear to be endemic in southwestern Japan and the Caribbean. This cross-sectional population-based study was conducted to describe the seroepidemiology of HTLV in the Repub
Ann Intern Med. 1990 May 15;112(10):727-37. Unique Identifier : AIDSLINE MED/90233579
Fischl MA; Richman DD; Hansen N; Collier AC; Carey JT; Para MF; Hardy WD; Dolin R; Powderly WG; Allan JD; et al; University of Miami School of Medicine, Department of Medicine,; FL 33101.
OBJECTIVE: To evaluate the efficacy and safety of zidovudine early in the treatment of human immunodeficiency virus type 1 (HIV) infection. DESIGN: A double-blind, randomized, placebo-controlled trial with subject stratification by pretreatment CD4 T lymphocyte counts. SETTING: Multicenter trial at AIDS Clinical Trial
Brunkhorst U; Sturner M; Willers H; Deicher H; Schedel I; Medizinische Hochschule, Zentrum Innere Medizin, Hannover, FR; Germany.
40 adults with symptomatic HIV-1 infection (AIDS related complex [ARC] WR 2B-4B or AIDS WR 5-6) were randomized into two groups, receiving either 200 mg of an i.v. immunoglobulin preparation (ivIg)/kg body weight every other week or no such treatment. Medical care and antibiotic therapy were comparable in the two grou
McSharry JJ; Costantino R; Robbiano E; Echols R; Stevens R; Lehman JM; Department of Microbiology, Albany Medical College, New York; 12208.
A flow cytometric assay has been developed to detect and quantitate human immunodeficiency virus (HIV)-infected peripheral blood mononuclear cells obtained from HIV-seropositive patients. Peripheral blood was obtained from patients attending an acquired immune deficiency syndrome clinic, and mononuclear cells were sep
J Immunol. 1990 May 15;144(10):3779-83. Unique Identifier : AIDSLINE MED/90237487
Willerford DM; Gale MJ Jr; Benveniste RE; Clark EA; Gallatin WM; Division of Basic Sciences, Fred Hutchinson Cancer Research; Center, Seattle, WA 98104.
HIV and the related simian immunodeficiency virus (SIV), which causes AIDS in macaques, infect only a small percentage of CD4+ lymphocytes at any point during the disease. We have identified three distinct cellular phenotypes within the CD4+ subpopulation in macaques, based on cell surface expression of CD44 and CD45R
Chappuis BB; Muller H; Stutte J; Hey MM; Hubner K; Muller-Hermelink HK; Institute of Pathology, University of Wurzburg, Federal Republic; of Germany.
Fourteen examples of non-Hodgkin s lymphoma (NHL) and four of Hodgkin s disease in patients with AIDS as well as lymph nodes exhibiting changes related to the lymphadenopathy syndrome (LAS) from 11 HIV-positive individuals were studied for the presence of Epstein-Barr virus (EBV) g
Philos Trans R Soc Lond B Biol Sci. 1990 Apr 12;327(1241):577-83. Unique Identifier : AIDSLINE MED/90245901
Moray N; Department of Mechanical and Industrial Engineering, University; of Illinois, Urbana-Champaign 61801.
Lattice theory is proposed to provide a formalism for the knowledge base used as a mental model by the operator of a complex system. The ordering relation greater than or equal to is interpreted as is caused by , and the lattice becomes a representation of the operator s causal hypotheses about the system. A given
Bagnara GP; Zauli G; Giovannini M; Re MC; Furlini G; La Placa M; Institute of Histology and General Embryology, University of; Bologna, Italy.
We investigated the in vitro growth of circulating progenitors from mononuclear nonadherent cells (MNAC) and T-depleted MNAC (non-T-MNAC) in the peripheral blood (PB) of 20 human immunodeficiency virus type 1 (HIV-1) seropositive subjects, compared with 12 normal adult volunteers, in order to clarify whether the loss
Perronne C; Lazanas M; Bellou A; Leport C; Canton P; Vilde JL; Service des Maladies infectieuses et tropicales, Hopital; Claude-Bernard, Paris.
Between January 1981 and April 1989, 50 patients infected with HIV were examined for herpes zoster. Herpes zoster enabled HIV infection to be detected in 23 patients (46 percent). It had only one localisation (involving contiguous dermatomes) in 37 patients, two localisations in 6 patients, three or four localisations
Salmon D; Lambin P; Courouce AM; Lefrere JJ; Doinel C; Rouger P; Salmon C; Institut National de Transfusion Sanguine, Universite Pierre et; Marie Curie, Paris.
Among the HIV-1 seropositive subjects detected through blood donation in Paris area who every six months voluntarily went through a thorough clinical and biological follow-up in the Institut National de Transfusion Sanguine, a cohort of 77 subjects had completed at least four biannual controls by september 1988. Upon
AIDS Res Hum Retroviruses. 1990 Mar;6(3):341-56. Unique Identifier : AIDSLINE MED/90253922
Sawyer LA; Katzenstein DA; Hendry RM; Boone EJ; Vujcic LK; Williams CC; Zeger SL; Saah AJ; Rinaldo CR Jr; Phair JP; et al; Laboratory of Retrovirus Research, Food and Drug Administration,; Bethesda, MD 20892.
We studied the relationship between early human immunodeficiency virus type 1 (HIV-1) specific immune responses and pathogenesis of infection in participants enrolled in the multicenter AIDS cohort study (MACS). Sera collected at 6-month intervals for 2 years (visit 1-5) from 39 persons who seroconverted by enzyme-lin
Nokta MA; Pollard RB; Department of Internal Medicine, University of Texas Medical; Branch, Galveston 77550.
A number of immune parameters have been described as impaired in AIDS patients. The patterns of interferon-gamma (IFN-gamma) production by peripheral blood mononuclear cells (PBMC) from AIDS-related complex (ARC) and AIDS patients in response to specific and nonspecific mitogens and their relationship to proliferative
Lancet. 1990 Jun 2;335(8701):1307-9. Unique Identifier : AIDSLINE MED/90258601
Presant CA; Blayney D; Proffitt RT; Turner AF; Williams LE; Nadel HI; Kennedy P; Wiseman C; Gala K; Crossley RJ; et al; Wilshire Oncology Medical Group, Los Angeles Oncologic Institute,; Los Angeles, California 91790.
Kaposi sarcoma and malignant lymphoma were successfully imaged with high purity liposomes containing indium-111 in two patients with AIDS. Gamma camera images of the patient with Kaposi sarcoma showed four discrete lesions along the left foot and calf with no foci along the right foot, and no uptake into clinically en
Pantazis P; Kalyanaraman VS; Bing DH; Center for Blood Research, Harvard Medical School, Boston,; Massachusetts 02115.
The third component of complement (C3) plays key roles in complement activation of both the classical and alternative pathways. The liver is the major site of C3 synthesis; monocytes, B-lymphocytes and leukemic cell lines of the myeloid lineage also synthesize C3. Here we report that the C3 gene is inactive in fresh T
Ann Intern Med. 1990 Jun 1;112(11):805-11. Unique Identifier : AIDSLINE MED/90262042
Lane HC; Davey V; Kovacs JA; Feinberg J; Metcalf JA; Herpin B; Walker R; Deyton L; Davey RT Jr; Falloon J; et al; National Institutes of Health, Bethesda, Maryland.
STUDY OBJECTIVE: To evaluate the toxicity and clinical efficacy of interferon-alpha 2b (IFN-alpha) in patients with asymptomatic human immunodeficiency virus (HIV) infection. DESIGN: Randomized, placebo-controlled, and double-blind study. SETTING: Outpatient clinic of a government referral-based research hospital. PAT
Ann Intern Med. 1990 Jun 1;112(11):812-21. Unique Identifier : AIDSLINE MED/90262043
Krown SE; Gold JW; Niedzwiecki D; Bundow D; Flomenberg N; Gansbacher B; Brew BJ; Memorial Sloan-Kettering Cancer Center, New York, New York.
OBJECTIVE: To evaluate safety, tolerance, and potential efficacy of interferon-alpha and zidovudine combination therapy in patients with Kaposi sarcoma and the acquired immunodeficiency syndrome (AIDS). DESIGN: Open, phase-I study with randomization between two prepara
AIDS Res Hum Retroviruses. 1990 Feb;6(2):205-17. Unique Identifier : AIDSLINE MED/90226890
Lai PK; Donovan J; Takayama H; Sakagami H; Tanaka A; Konno K; Nonoyama M; Department of Virology, Tampa Bay Research Institute, St.; Petersberg, FL 33716.
We have shown previously that two fractions (PC6 and PC7) extracted from cones of the Japanese white pine Pinus parvifloria Sieb. et Zucc have potent immunopotentiating effects. Here, we show that PC6 and PC7 inhibited HIV-1 replication (greater than 95%), in a dose-dependent manner, in chronically infected CR10/HIV-1
EMBO J. 1990 May;9(5):1583-93. Unique Identifier : AIDSLINE MED/90228359
Ricchetti M; Buc H; Institut Pasteur, Unite de Physicochimie des Macromolecules; Biologiques, (URA1149 du CNRS), Paris, France.
Kinetics of incorporation of correct and incorrect deoxynucleotides by three reverse transcriptases have been followed, by gel assay, on a series of DNA templates, including part of the HIV-1 gag DNA minus strand. Insertion kinetics for the properly matched nucleotide at a given place on the template vary strongly fro
J Immunol. 1990 May 1;144(9):3385-91. Unique Identifier : AIDSLINE MED/90229725
Yamamoto H; Miller MD; Tsubota H; Watkins DI; Mazzara GP; Stallard V; Panicali DL; Aldovini A; Young RA; Letvin NL; Harvard Medical School, New England Regional Primate Research; Center, Southborough, MA 01772.
CD8+ CTL inhibit the replication of HIV and simian immunodeficiency virus of macaques (SIVmac) in PBL and, therefore, are likely to play an important role in containing the spread of the AIDS virus in infected individuals. We have generated a series of gag-specific lytic T lymphocyte clones from PBL: of an SIVmac-infe
Rooke R; Tremblay M; Wainberg MA; Lady Davis Institute--Jewish General Hospital, Montreal, Quebec,; Canada.
The reverse transcriptase enzyme of HIV-1 is known to be error-prone. We were interested in the possibility of isolating a variant HIV-1 strain that might be capable of replication in the presence of AZT , thought to act by antagonizing reverse transcr
Sommerfelt MA; Weiss RA; Chester Beatty Laboratories, Institute of Cancer Research,; London, United Kingdom.
The types of receptors on the surfaces of human and other mammalian cells for 13 C-type and 7 D-type retrovirus strains were determined by interference to the formation of syncytia and the plating of viral pseudotypes. All the D-type simian retroviruses (SRV-1-5, SMRV, PO-1-Lu) share a common receptor which is also ut
Biochemistry. 1990 Feb 20;29(7):1764-9. Unique Identifier : AIDSLINE MED/90234690
Luke W; Hoefer K; Moosmayer D; Nickel P; Hunsmann G; Jentsch KD; German Primate Centre, Gottingen.
The reverse transcriptase (RT) was partially purified by a newly developed procedure from the simian immunodeficiency virus TYO-7 isolated from an African green monkey (SIVagmTYO-7). The method comprised lysis of the virus with nonionic detergent followed by two centrifugations in isopycnic sucrose density gradients a
Kohsaka H; Yamamoto K; Fujii H; Miura H; Miyasaka N; Nishioka K; Miyamoto T; Department of Medicine and Physical Therapy, Faculty of Medicine,; University of Tokyo, Japan.
To analyze the autoepitopes on the SS-B/La protein, a cDNA covering the entire region coding the protein was isolated from a human cDNA library. The cDNA was subcloned into an expression plasmid vector, pEX, to express its protein product as a fusion protein with cro-beta-galactosidase in Escherichia coli. A recombina
A man with previous addiction to parenteral drugs presented infection by human immunodeficiency virus (HIV) with persistent generalised lymph node enlargement. Sixteen months after diagnosis, he developed intermediate-grade lymphoma of the stomach. Partial response was achieved by chemotherapy, and the patient present
Am Fam Physician. 1990 May;41(5):1521-8. Unique Identifier : AIDSLINE MED/90240161
Brogan KL; Zell SC; University of Nevada School of Medicine, Reno.
Zidovudine is now used extensively in an effort to control infection with the human immunodeficiency virus (HIV). The drug is associated with major hematologic toxicity, especially anemia and granulocytopenia. Conservative management of hematologic toxicity includes dosage reduction or cessation of therapy, diagnosis
Smith AJ; Cho MI; Hammarskjold ML; Rekosh D; Department of Microbiology, State University of New York, Buffalo; 14214.
Human immunodeficiency virus type 1 (HIV-1) gag and pol genes were expressed by using fragments of the BH10 clone of HIV inserted into a simian virus 40 late replacement vector. An initial construct containing the entire coding regions of gag, pol, and vif produced only minute amounts of the gag precursor, Pr55gag. Ho
Carbohydrate structures are often involved in the initial adhesion of pathogens to target cells. In the present study, a panel of anticarbohydrate monoclonal antibodies (MAbs) was tested for their ability to inhibit in vitro human immunodeficiency virus infectivity. MAbs against three different N- and O-linked carbohy
Am J Med. 1990 May 21;88(5B):2S-7S. Unique Identifier : AIDSLINE MED/90247433
Broder S; National Cancer Institute, National Institutes of Health,; Bethesda, Maryland 20892.
The incidence of acquired immunodeficiency syndrome and the number of people infected with the human immunodeficiency virus (HIV) is likely to increase into the 1990s and perhaps beyond. Zidovudine, a 2 ,3 -dideoxynucleoside approved for the treatment of acquired immunodeficiency syndrome, provides immunologic, virolo
Orenstein JM; Chiang J; Steinberg W; Smith PD; Rotterdam H; Kotler DP; Department of Pathology, George Washington University Medical; Center, Washington, DC 20037.
Chronic diarrhea accompanied by weight loss is a common and often debilitating problem associated with human immunodeficiency virus (HIV) infection. Enterocytozoon bieneusi, a newly identified species of the phylum of protozoa, Microspora, has been reported associated with chronic diarrhea and wasting in 11 acquired i
Schinella RA; Greco MA; Department of Pathology, Bellevue Hospital, New York University; School of Medicine, NY.
A patient with human immunodeficiency virus infection presented with a soft-tissue mass which histologically and clinically mimicked an angiosarcoma. Ultrastructural study, however, revealed bacteria identical to those seen in cutaneous bacillary angiomatosis , but the patient
Montefiori DC; Robinson WE; Modliszewski A; Rowland JM; Schuffman SS; Mitchell WM; Department of Pathology, Vanderbilt University, School of; Medicine, Nashville, Tennessee 37232.
Dextran sulphate (MW 5000 and 8000) and a polysulphated glycosaminoglycan (MW 10,000), at concentrations that provided complete protection in a homologous infection assay, failed to block syncytium formation and the resulting cytopathic effect when MT-2 cells were mixed with H9/HIV-1 cells. These substances also had n
Lisse IM; Whittle H; Aaby P; Normark M; Gyhrs A; Ryder LP; Department of Pathology, Hvidovre Hospital, Copenhagen, Denmark.
Immuno-alkaline phosphatase (AP) staining for T cell subsets (CD4 and CD8) of smears from fingerprick blood functioned well under tropical climatic conditions when smears were stored frozen with silica gel before being labelled. Unlabelled smears were stored for up to 12 months and could be transferred abroad without
AIDS Res Hum Retroviruses. 1990 Mar;6(3):287-98. Unique Identifier : AIDSLINE MED/90253917
De Rossi A; Calabro ML; Panozzo M; Bernardi D; Caruso B; Tridente G; Chieco-Bianchi L; Institute of Oncology, University of Padova, Italy.
To ascertain whether thymic lymphocytes represent suitable targets for HIV-1 infection, we infected thymic cell suspensions from normal donors with HIV-1 (HTLV-IIIB strain). We found that, in vitro, thymic lymphocytes are readily infected and highly permissive for HIV-1 replication. In addition, immature cells with th
AIDS Res Hum Retroviruses. 1990 Mar;6(3):317-27. Unique Identifier : AIDSLINE MED/90253920
Marcus-Sekura CJ; Woerner AM; Zhang PF; Klutch M; Division of Virology, Food and Drug Administration, Bethesda, MD; 20892.
Immune response to HIV infection is generally characterized by appearance of antibodies to the gag protein p24 early in infection, and by apparent loss of p24 antibodies accompanied by increases in p24 antigen levels with disease progression. Precise definition of the immunodominant epitopes present in gag gene protei
We engineered a prokaryotic expression vector encoding the HIV reverse transcriptase (RT). We grew Escherichia coli JM109 carrying the vector in a 250-liter stirred tank fermentor and purified RT (p66) under native conditions to apparent homogeneity. Purified p66 (greater than or equal to 5 mg/ml) was not stable, and
AIDS Res Hum Retroviruses. 1990 Mar;6(3):381-92. Unique Identifier : AIDSLINE MED/90253925
Teeuwsen VJ; Siebelink KH; Crush-Stanton S; Swerdlow B; Schalken JJ; Goudsmit J; van de Akker R; Stukart MJ; Uytdehaag FG; Osterhaus AD; National Institute of Public Health and Environmental Protection,; Laboratory of Immunobiology, Bilthoven, The Netherlands.
A human Epstein-Barr virus-transformed lymphoblastoid B-cell line was generated from peripheral blood mononuclear cells (PBMC) of an asymptomatic human immunodeficiency virus type I (HIV-1) seropositive donor, which produces a human monoclonal antibody K14 (IgG1), reactive with an epitope on the transmembrane part (gp
AIDS Res Hum Retroviruses. 1990 Mar;6(3):401-10. Unique Identifier : AIDSLINE MED/90253927
Uriel J; Lunardi-Iskandar Y; Laborda J; Torres JM; Naval J; Georgoulias V; Lowi R; Jasmin C; Mishal Z; Laboratoire de Chimie des Proteines, Institut de Recherches; Scientifiques sur le Cancer, Villejuif, France.
Serum alpha-fetoprotein (AFP) and transferrin (Tf) are actively endocytosed by many growing cells during ontogenic and neoplastic growth, but also by peripheral T lymphocytes upon mitogen activation. AFP and Tf uptake occurs through receptor-mediated endocytosis. The purpose of the present work was to assess whether t
AIDS Res Hum Retroviruses. 1990 Mar;6(3):411-6. Unique Identifier : AIDSLINE MED/90253928
Vasudevachari MB; Mast TC; Salzman NP; Division of Molecular Virology and Immunology, Georgetown; University School of Medicine, Washington, DC 20007.
The detection of HIV-1 in human peripheral blood lymphocytes is routinely carried out by cocultivation of test cells with normal peripheral blood mononuclear cells (PBMC). The presence of virus is evidenced by cytologic observation of syncytia or by detecting viral reverse transcriptase (RT) and/or p24 antigen in the
Studies on monitoring the immune response to viral structural proteins during human immunodeficiency virus (HIV-1) infection have established the significance of antibodies to the core protein p24 during the progression of the disease. We have studied the prevalence of antibodies to the core protein p17 in order to st
AIDS Res Hum Retroviruses. 1990 Apr;6(4):481-9. Unique Identifier : AIDSLINE MED/90253936
Tsai WP; Nara PL; Kung HF; Oroszlan S; Laboratory of Biochemical Physiology, NCI-Frederick Cancer; Research Facility, Frederick, MD 21701.
The effect of chloroquine, a drug known to affect intracellular exocytic pathways, was studied in two retroviral systems: human immunodeficiency virus (HIV-1) and avian reticuloendotheliosis virus (REV-A). With chloroquine treatment of virus-infected cells, significant size reduction of the cell- and virus-associated
AIDS Res Hum Retroviruses. 1990 Apr;6(4):491-501. Unique Identifier : AIDSLINE MED/90253937
Kucera LS; Iyer N; Leake E; Raben A; Modest EJ; Daniel LW; Piantadosi C; Department of Microbiology, Bowman Gray School of Medicine, Wake; Forest University, Winston-Salem, NC 27103.
A new class of membrane-active ether lipid (EL) analogs of platelet-activating factor were studied for in vitro anti-HIV-1 activity. Human T-cell (CEM-ss) monolayers or suspension cultures were used to determine effects of structural modifications of Type A phosphorus-containing and Type B nonphosphorus EL analogs on
Kedar PS; Abbotts J; Kovacs T; Lesiak K; Torrence P; Wilson SH; Laboratory of Biochemistry, National Cancer Institute, Bethesda,; Maryland 20892.
Primer and dNTP recognition by purified HIV reverse transcriptase have been investigated. Earlier kinetic studies suggested that the reaction pathway for DNA synthesis is ordered, with template-primer and free enzyme combining to form the first complex in the reaction sequence [Majumdar et al. (1988) J. Biol. Chem. 26
Adult C57BL/10 mice (H-2b Fv-1b) inoculated with LP-BM5 murine leukemia virus develop a disease which has many features in common with human acquired immunodeficiency syndrome (AIDS), in particular abnormal lymphoproliferation and severe immunodeficiency. In the present study, we examined the possibility that this mur
Mansuri MM; Hitchcock MJ; Buroker RA; Bregman CL; Ghazzouli I; Desiderio JV; Starrett JE; Sterzycki RZ; Martin JC; Department of Antiinfective Chemistry, Bristol-Myers Squibb; Company, Wallingford, Connecticut 06492-7660.
Three analogs of thymidine, D4T [2 ,3 -didehydro-2 ,3 -dideoxythymidine; 1-(2,3-dideoxy-beta-D-glyceropent-2-enofuranosyl)thymine], FddT (3 -fluoro-3 -deoxythymidine), and AZT (3 -azido-3 -deoxythymidine), were compared in biological tests designed to
This information is designed to support, not replace, the relationship that exists between you and your doctor.