Relationship of human cord blood mononuclear cell lines, established by the HTLV-I integration, to expression of T-cell phenotypes and the T-cell receptor gene rearrangement.

Important note: Information in this article was accurate in 1989. The state of the art may have changed since the publication date.

Relationship of human cord blood mononuclear cell lines, established by the HTLV-I integration, to expression of T-cell phenotypes and the T-cell receptor gene rearrangement.

J Mol Cell Immunol. 1987;3(1):43-8. Unique Identifier : AIDSLINE MED/89322721
Miwa H; Uchida T; Kobayashi T; Kita K; Shirakawa S; Koyanagi Y; Yamamoto N; Shimizu A; Honjo T; Hatanaka M; Institute for Virus Research, Kyoto University, Japan.

Abstract: The HTLV-I integrated cell lines originated from human cord blood mononuclear cells were examined for the T-cell receptor beta chain gene rearrangement in conjunction with immunological phenotype analysis. Five of seven cell lines contained the rearranged T-cell receptor beta chain gene. Interestingly, most of the rearranged lines failed to express the T cell differentiation antigens, OKT 4 and 8, though their positive expression of Leu 1 antigen indicated the commitment to the T-cell lineage. On the other hand, two cell lines retained the germ-line configuration of the beta chain gene, while phenotypical examination revealed that the two cell lines have distinct T-cell antigens. Non-rearranged cell lines have proved to be nearly monoclonal from HTLV-I integration pattern and/or surface marker clonality. Thus, the relationship between the immunological phenotypes and the T-cell receptor gene rearrangement observed in the T-cell lineage is not strict and often dissociates. A possible involvement of HTLV-I infection on the dissociation between phenotype and genotype is discussed from the viewpoint of phenotypical modulation.

Keywords: Antigens, Surface/GENETICS Cell Line, Transformed Fetal Blood/IMMUNOLOGY *Gene Rearrangement, beta-Chain T-Cell Antigen Receptor Human HTLV-I/GENETICS Leukocytes, Mononuclear/IMMUNOLOGY Phenotype Support, Non-U.S. Gov't T-Lymphocytes/*IMMUNOLOGY JOURNAL ARTICLE

891130
M89B0545

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