Relative importance of total versus external platelet-associated IgG. NLM AIDSLINE Important note: Information in this article was accurate in 1989. The state of the art may have changed since the publication date.

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Relative importance of total versus external platelet-associated IgG.

Blut. 1989 Jul;59(1):67-74. Unique Identifier : AIDSLINE MED/89323515
Nydegger UE; Buchs JP; Borradori L; Central Laboratory of Haematology, University of Bern,; Switzerland.

Abstract: We present measurements of total platelet-associated immunoglobulin G following platelet lysis (PAIgG tot) and surface-restricted IgG (PAIgG ext) on intact, gel-filtered platelets from 36 normal human donors and 9 patients with human immunodeficiency virus (HIV) infection (CDC stages IIb-IV). For this purpose, an indirect micro ELI-SA technique was developed involving competition between PAIgG and solid-phase absorbed IgG for fixation of conjugated anti-human IgG antibody. In normal donors, the mean values of PAIgG tot was 8.3 +/- 7.4 (mean +/- 2 SD) and of PAIgG ext 4.2 +/- 4.4 fg/platelet. In HIV-infected subjects, PAIgG tot was 37.2 +/- 62.8 and PAIgG ext 17.1 +/- 23 fg/platelet. In healthy subjects the comparison of individual levels of PAIgG tot and PAIgG ext revealed a significant correlation (r:0.763; p: 0.003). These results are compared to those which have been reported in the literature. In addition, the major immunopathological mechanisms considered responsible for immune-mediated idiopathic thrombocytopenic purpura are discussed.
Keywords: Blood Platelets/*IMMUNOLOGY Endocytosis Enzyme-Linked Immunosorbent Assay/METHODS Human HIV/*IMMUNOLOGY IgG/*IMMUNOLOGY Purpura, Thrombocytopenic/IMMUNOLOGY Support, Non-U.S. Gov't JOURNAL ARTICLE

KWDbloodplatelets/KWDimmunologyendocytosisenzyme-linkedimmunosorbentassay/methodshumanhiv/KWDimmunologyigg/KWDimmunologypurpura,thrombocytopenic/immunologysupport,non-uKWDsKWDgov'tjournalarticle
891130
M89B0532

Copyright © 1989 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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