PERSISTENT IN VITRO INFECTION OF MACROPHAGES BY THE HUMAN IMMUNODEFICIENCY VIRUS

Important note: Information in this article was accurate in 1989. The state of the art may have changed since the publication date.

PERSISTENT IN VITRO INFECTION OF MACROPHAGES BY THE HUMAN IMMUNODEFICIENCY VIRUS

Vaccines 88. New Chemical and Genetic Approaches to Vaccination: Prevention of AIDS and Other Viral, Bacterial, and Parasitic Diseases. Ginsberg H et al, eds. New York, Cold Spring Harbor Laboratory, p. 341-5, 1988.. Unique Identifier : AIDSLINE ICDB/89649779
Gendelman HE; Martin MA; Orenstein J; Phipps T; Meltzer MS; Lab. of Molecular Microbiology, NIAID, NIH, Bethesda, MD 20892

Abstract: HIV often leads to clinically overt disease following latency periods of months to years. Virus continually replicates in cells of the monocyte/macrophage lineage despite an often vigorous but ineffective host immune response. To study persistent HIV infection in macrophages, a system was developed for long-term maintenance of primary blood-derived monocytes. The blood monocytes were maintained for intervals of more than 2 mo in medium supplemented with human colony-simulating factor 1 (rCSF-1). The cells were susceptible to HIV infection, producing progeny virions for up to 50 days following infection. The treatment of macrophages isolated from peripheral blood with rCSF-1 allows both increased viability and efficient propagation of HIV. These stimulated macrophages efficiently produce large numbers of progeny virions that are sequestered into cytoplasmic vacuoles. The paucity of associated virus on the plasma membrane of cells suggests one way infected cells may escape a competent host immune response. In addition, the intracellular retention of HIV particles in macrophages likely explains the discordance between the low extracellular reverse transcriptase and the numbers of infected cells. The in vivo presence of HIV-infected macrophages that sequester virions may reflect aspects of latency and virus dissemination seen during the course of disease. (5 Refs)

Keywords: Cells, Cultured Human HIV/*PHYSIOLOGY/ULTRASTRUCTURE Inclusion Bodies, Viral/ULTRASTRUCTURE Macrophages/*MICROBIOLOGY Microscopy, Electron *Virus Replication MEETING PAPER

890330
M8930535

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1989. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1989. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .