Use of non-radioactive DNA probes for the characterization of adult T-cell leukemia cells. NLM AIDSLINE Important note: Information in this article was accurate in 1989. The state of the art may have changed since the publication date.

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Use of non-radioactive DNA probes for the characterization of adult T-cell leukemia cells.

Nucleic Acids Symp Ser. 1988;(19):77-80. Unique Identifier : AIDSLINE MED/89144970
Moriuchi T; Koji T; Nakane PK; Yoshida M; Moriuchi J; Arimori S; Department of Cell Biology, Tokai University School of Medicine,; Isehara, Japan.

Abstract: DNA fragments were labeled with dinitrophenyl (DNP) residues by the reaction with 2,4-dinitrobenzaldehyde in alkaline condition and the labeled DNA was used as a probe for non-radioactive in situ hybridization. DNP-labeled DNA probes for T cell receptor beta chain, c-myc and HTLV-1 were hybridized in situ to mRNA on cell specimens fixed with Carnoy's fixative. DNA-mRNA hybrids were detected immunohistochemically using anti-DNP antibodies. Cytoplasms of adult T cell leukemia cells were stained with varied intensity when these probes were used. More than 70% of cells were positively stained with T cell receptor probe. However, less than 30% of cells were stained with c-myc and HTLV-1 probes. The present study indicates that non-radioactive in situ hybridization can be used for the characterization and classification of leukemia.
Keywords: Animal DNA/*ANALYSIS *DNA Probes DNA, Viral/ANALYSIS Genes, Structural Human HTLV-I/GENETICS Leukemia, T-Cell/*GENETICS Mice Nucleic Acid Hybridization Plasmids *Proto-Oncogenes Receptors, Antigen, T-Cell/GENETICS Support, Non-U.S. Gov't JOURNAL ARTICLE

KWDanimaldna/KWDanalysisKWDdnaprobesdna,viral/analysisgenes,structuralhumanhtlv-i/geneticsleukemia,t-cell/KWDgeneticsmicenucleicacidhybridizationplasmidsKWDproto-oncogenesreceptors,antigen,t-cell/geneticssupport,non-uKWDsKWDgov'tjournalarticle
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M8960555

Copyright © 1989 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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