Simultaneous quantification of zidovudine and its glucuronide in serum by high-performance liquid chromatography. NLM AIDSLINE Important note: Information in this article was accurate in 1989. The state of the art may have changed since the publication date.

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Simultaneous quantification of zidovudine and its glucuronide in serum by high-performance liquid chromatography.

J Chromatogr. 1988 Sep 23;431(1):123-33. Unique Identifier : AIDSLINE MED/89175180
Good SS; Reynolds DJ; de Miranda P; Experimental Therapy Department, Wellcome Research Laboratories,; Research Triangle Park, NC 27709.

Abstract: A sensitive high-performance liquid chromatography (HPLC) assay has been developed to simultaneously determine levels of the anti human immunodeficiency virus agent, zidovudine (AZT), and its major metabolite (the 5'-O-glucuronide) in serum. Samples were first mixed with an internal standard (a stereoisomer of AZT), then prepared for analysis using solid-phase extraction columns and chromatographed using a reversed-phase analytical column. Isocratic elution with a mobile phase of 15% acetonitrile, buffered to pH 2.70 with ammonium phosphate, gave good resolution of the three analytes and endogenous serum components. The HPLC analysis time required per sample was 34 min and analyte recoveries were reproducibly high (greater than 93%). Replicate analyses of prepared standards gave satisfactory precision and accuracy, with coefficients of variation less than 15% and deviations from expected concentrations less than 10%.
Keywords: Acquired Immunodeficiency Syndrome/BLOOD/DRUG THERAPY AIDS-Related Complex/BLOOD/DRUG THERAPY Chromatography, High Pressure Liquid Glucuronates/BLOOD Human Hydrogen-Ion Concentration Indicators and Reagents Zidovudine/*BLOOD/THERAPEUTIC USE JOURNAL ARTICLE

KWDacquiredimmunodeficiencysyndrome/blood/drugtherapyaids-relatedcomplex/blood/drugtherapychromatography,highpressureliquidglucuronates/bloodhumanhydrogen-ionconcentrationindicatorsandreagentszidovudine/KWDblood/therapeuticusejournalarticle
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M8970720

Copyright © 1989 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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