High-performance liquid chromatographic determination of fusidic acid in plasma. NLM AIDSLINE Important note: Information in this article was accurate in 1989. The state of the art may have changed since the publication date.

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High-performance liquid chromatographic determination of fusidic acid in plasma.

J Chromatogr. 1988 Dec 9;433:159-66. Unique Identifier : AIDSLINE MED/89175210
Rahman A; Hoffman NE; Marquette University, Milwaukee, WI 53233.

Abstract: Fusidic acid was determined in plasma by a high-performance liquid chromatographic method. Fusidic acid was extracted from plasma with acetonitrile that was salted out with ammonium sulfate. Prior to salting out cadmium sulfate was mixed with the acetonitrile-plasma mixture to help remove interfering constituents. A 150 mm X 4.6 mm column packed with 5-microns cyanopropyl stationary phase was used for chromatography. The mobile phase was acetonitrile-20 mM sodium dihydrogenphosphate (pH 3.50) (39:61, v/v). An ultraviolet-visible detector was set at 204 nm. The presence of water in the injection solvent had a significant effect on the fusidic acid peak height. A number of clinically important acquired immunodeficiency syndrome drugs did not interfere with the fusidic acid determination. The relative standard deviation varied between 0.99 and 7.8%. A limit of detection of 200 ng/ml was obtained for a 80-microliters injection.
Keywords: Chromatography, High Pressure Liquid Fusidic Acid/*BLOOD Human Spectrophotometry, Ultraviolet JOURNAL ARTICLE

KWDchromatography,highpressureliquidfusidicacid/KWDbloodhumanspectrophotometry,ultravioletjournalarticle
890730
M8970719

Copyright © 1989 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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