Comparison of sensitivities and specificities of latex agglutination and an enzyme-linked immunosorbent assay for detection of antibodies to the human immunodeficiency virus in African sera. NLM AIDSLINE Important note: Information in this article was accurate in 1989. The state of the art may have changed since the publication date.

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Comparison of sensitivities and specificities of latex agglutination and an enzyme-linked immunosorbent assay for detection of antibodies to the human immunodeficiency virus in African sera.

J Clin Microbiol. 1988 Nov;26(11):2462-4. Unique Identifier : AIDSLINE MED/89175419
Francis HL; Kabeya M; Kafuama N; Riggins C; Colebunders R; Ryder R; Curran J; Izaley L; Quinn TC; Project SIDA, Blood Bank Center, Mama Yemo Hospital, Kinshasa,; Zaire.

Abstract: The sensitivities, specificities, and positive and negative predictive values of the Cambridge BioScience Corp. (Worcester, Mass.) human immunodeficiency virus latex agglutination assay were compared by using three different blood preparations. By using the manufacturer's standard test method with diluted sera, the sensitivity of latex agglutination was 100%, the specificity was 99.58%, and the positive and negative predictive values were 99.26 and 100%, respectively. Use of diluted whole blood or undiluted whole blood did not significantly affect the sensitivity (mean, 99.72%), specificity (mean, 99.47%), positive predictive value (mean, 99.07%), or negative predictive value (mean, 99.89%). The latex agglutination assay is a simple, rapid assay for the detection of human immunodeficiency virus that would be useful in Third World countries or other areas where enzyme-linked immunosorbent assays are not available or cannot be used.
Keywords: Acquired Immunodeficiency Syndrome/*DIAGNOSIS Blotting, Western Comparative Study Enzyme-Linked Immunosorbent Assay Human HIV Antibodies/*ANALYSIS Latex Fixation Tests Zaire JOURNAL ARTICLE

KWDacquiredimmunodeficiencysyndrome/KWDdiagnosisblotting,westerncomparativestudyenzyme-linkedimmunosorbentassayhumanhivantibodies/KWDanalysislatexfixationtestszairejournalarticle
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M8970717

Copyright © 1989 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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