STUDY OF NATURAL CELL-MEDIATED CYTOTOXICITY TO K562 CELL-LINE IN ACQUIRED IMMUNODEFICIENCY SYNDROME NLM AIDSLINE Important note: Information in this article was accurate in 1989. The state of the art may have changed since the publication date.

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STUDY OF NATURAL CELL-MEDIATED CYTOTOXICITY TO K562 CELL-LINE IN ACQUIRED IMMUNODEFICIENCY SYNDROME

Diss Abstr Int [B]; 48(10):2915 1988. Unique Identifier : AIDSLINE ICDB/89647816
Baron GC; Univ. of Miami

Abstract: Natural cell-mediated cytotoxicity (CYT) was determined using whole blood samples from patients (pts) with the acquired immunodeficiency syndrome (AIDS) and AIDS-related complexes (ARC) and correlated to the proportion and absolute number of natural killer (NK) cells as determined by monoclonal antibodies Leu 11 or NKH.1 and flow cytometry. Mean percent CYT was significantly lower in AIDS and ARC pts compared with that of normal controls, and the defect observed in these pts was not due to fewer NK cells or decreased efficiency in binding to target cells. Kinetic analysis of CYT showed that NK cells from AIDS or ARC pts lysed fewer cells than NK cells from normals. NK cells in these pts are in a less active state or exhibit decreased recycling. Blood samples from the majority of pts with AIDS or ARC were refractory to the stimulatory effects of in vitro additions of interferon alpha (IFNA), interferon gamma (IFNG), or interleukin 2 on CYT. Some AIDS/KS pts receiving in vivo IFNA had increased CYT after therapy, and pts receiving in vivo IFNG became responsive to IFNG. Humoral elements may be responsible in part for the reduced CYT observed in the pt groups. Elevated serum immunoglobulins and circulating immune complexes correlate inversely with CYT. Both factors inhibitory and stimulatory to CYT were present in fractionated plasma from an ARC pt. Capping studies suggest that there are alterations in membrane receptor mobility which correlate to diminished functional responses in AIDS and ARC pts. (Full text available from University Microfilms International, Ann Arbor, MI, as Order No: AAD87-29341)
Keywords: Acquired Immunodeficiency Syndrome/*IMMUNOLOGY AIDS-Related Complex/*IMMUNOLOGY Cell Line *Cytotoxicity, Immunologic Human Killer Cells, Natural/*IMMUNOLOGY THESIS

KWDacquiredimmunodeficiencysyndrome/KWDimmunologyaids-relatedcomplex/KWDimmunologycelllineKWDcytotoxicity,immunologichumankillercells,natural/KWDimmunologythesis
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Copyright © 1989 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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