Enzymatic amplification of HTLV-I viral sequences from peripheral blood mononuclear cells and infected tissues. NLM AIDSLINE Important note: Information in this article was accurate in 1989. The state of the art may have changed since the publication date.

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Enzymatic amplification of HTLV-I viral sequences from peripheral blood mononuclear cells and infected tissues.

Blood. 1988 Oct;72(4):1117-23. Unique Identifier : AIDSLINE MED/89001163
Kwok S; Ehrlich G; Poiesz B; Kalish R; Sninsky JJ; Cetus Corporation, Department of Diagnostic Research, Emeryville,; CA 94608.

Abstract: Human T-cell lymphotropic virus type I (HTLV-I) and human T-cell lymphotropic virus type II (HTLV-II) have been associated with adult T-cell leukemia/lymphoma (ATL) and a rare T-cell variant of hairy cell leukemia, respectively. Direct detection of viral nucleic acid in peripheral blood lymphocytes (PBLs) and infected tissues in carrier patients and those with chronic disease has proven refractory due to viral transcriptional dormancy and the small number of infected cells present. The investigators report here the successful application of the DNA amplification procedure, termed PCR, to the detection of these human oncoviruses. Judicious selection of specific oligonucleotides for primers and probes provides type-specific and simultaneous detection of these two retroviruses. The ability to amplify and detect highly conserved regions of these medically relevant viruses may facilitate the identification of, as yet, uncharacterized retroviruses.
Keywords: Base Sequence DNA Polymerases/*METABOLISM DNA, Viral/GENETICS/ISOLATION & PURIF/METABOLISM *Gene Amplification Human HTLV-I/*GENETICS HTLV-I Infections/*GENETICS Leukemia-Lymphoma, T-Cell, Acute, HTLV-I-Associated/*GENETICS Leukocytes, Mononuclear/*ANALYSIS Models, Genetic Molecular Sequence Data Oligonucleotide Probes Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE

KWDbasesequencednapolymerases/KWDmetabolismdna,viral/genetics/isolation&purif/metabolismKWDgeneamplificationhumanhtlv-i/KWDgeneticshtlv-iinfections/KWDgeneticsleukemia-lymphoma,t-cell,acute,htlv-i-associated/KWDgeneticsleukocytes,mononuclear/KWDanalysismodels,geneticmolecularsequencedataoligonucleotideprobessupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,non-pKWDhKWDsKWDsupport,uKWDsKWDgov't,pKWDhKWDsKWDjournalarticle
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M8910513

Copyright © 1989 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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