Expression, release, and characterization of soluble human T-lymphotropic virus-I (HTLV-I) antigens from an infected cell line. NLM AIDSLINE Important note: Information in this article was accurate in 1989. The state of the art may have changed since the publication date.

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Expression, release, and characterization of soluble human T-lymphotropic virus-I (HTLV-I) antigens from an infected cell line.

Cancer Detect Prev. 1988;12(1-6):31-7. Unique Identifier : AIDSLINE MED/89028478
Olsen RG; Lewis MG; Bauer RM; Tarr MJ; Mathes LE; Lafrado LJ; Blakeslee JR Jr; Department of Veterinary Pathobiology, College of Veterinary; Medicine, Ohio State University, Columbus 43210.

Abstract: The Human T-lymphotropic virus type I (HTLV-I) infected cell line MT-2 was studied to obtain HTLV-I or related proteins for the purpose of producing an effective vaccine for HTLV-I infection. The cells were characterized as to HTLV-I antigen expression during the cell cycle and antigens released into the culture fluids. MT-2 cell grown in fetal calf supplemented media produced more HTLV-I related antigens during the G2/M phase of the cell cycle. To determine the conditions for maximal release and harvest of HTLV-I associated proteins, the MT-2 cells were grown in RPMI 1640 medium supplemented with serum-free medium. Cell- and virus-free supernatants were collected on day 4, lyophilized, and concentrated 50-fold. The proteins in these supernatants were characterized using SDS-PAGE and western blot using rabbit anti-HTLV-I sera and human adult T-cell leukemia sera. The western-blot analysis indicated that the supernatants obtained from the MT-2 cells grown in serum-free supplemented medium contained detectable amounts of proteins which reacted with human ATL and rabbit anti-HTLV-I sera. The molecular weights of these proteins observed are 68kd, 46kd, 28kd, 24kd, 19kd, and 15kd indicating that gag, env, and pX gene products are present.
Keywords: Blotting, Western Cell Cycle Cell Line Electrophoresis, Polyacrylamide Gel Flow Cytometry Human HTLV-I Antigens/*ANALYSIS HTLV-I Infections/*IMMUNOLOGY Solubility Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE

KWDblotting,westerncellcyclecelllineelectrophoresis,polyacrylamidegelflowcytometryhumanhtlv-iantigens/KWDanalysishtlv-iinfections/KWDimmunologysolubilitysupport,uKWDsKWDgov't,pKWDhKWDsKWDjournalarticle
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Copyright © 1989 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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