Important note: Information in this article was accurate in 1989. The state of the art may have changed since the publication date.
IMMUNOHISTOCHEMICAL METHODS IN THE STUDIES OF MALIGNANT LYMPHOMAS AND IMMUNOPATHOLOGY OF HIV INFECTION
Diss Abstr Int [C]; 49(4):614 1989. Unique Identifier : AIDSLINE ICDB/89655154 Porwit A; Karolinska Institutet, Stockholm, Sweden
Abstract:
Monoclonal antibodies were applied in studies of benign lymphatic lesions, malignant lymphomas and HIV-related lymphadenopathy and also in HIV-encephalopathy using immunoenzymatic methods. In reactive lymph nodes and tonsils a distinct distribution of different subsets of T cells and NK-cells (Leu7+) was demonstrated, which possibly reflects their functional differences within the immunological system. In B cell lymphomas (NHL) a negative correlation was found between the percentage of cells in S-phase and the percentage of reactive T lymphocytes. A positive correlation was found between the proliferation index (percentage S-phase cells/percentage neoplastic cells in suspension) and reactivity for transferrin receptor. A high content of infiltrating T cells, particularly of the T suppressor/cytotoxic (Ts/c) phenotype, might be a favorable prognostic feature in highly malignant B-cell NHL. Immunohistochemical analysis of blood vessel distribution in B-cell derived NHL indicated that angiogenesis is usually related to the presence of nonmalignant T cells rather than to the lymphoma cell type and histological grade of malignancy. In addition to reactivity with endothelial cells, EN4 marker was found on neoplastic B cells of low-grade NHL (except CB/CC) and on numerous cells in perivascular cuffs. By double immunostaining, these EN4+ perivascular cells comprised a mixed population with a predominance of lymphocytes (mostly T-helper/inducer (Th/i)) and of some monocytes/macrophages. In HIV-related lymphadenopathy four stages were defined: follicular hyperplasia (FH); follicular involution with fragmentation (FF); follicular involution with atrophy (FA); and follicular depletion (FD). Significantly lower Th/i / Ts/c cell ratios in peripheral blood and lymph nodes were found in patients (pts) with FD by comparison with other histological groups. The majority of biopsies from pts with persistent generalized lymphadenopathy were morphologically classified as FH or FF, while the majority of biopsies from pts with manifest AIDS as FA or FD. In FH/FF a morphometric analysis of immunostained cryosections showed a significant increase in follicular dendritic cells in germinal centers, while a subsequent decrease was found in FA and FD. HIV antigens were also found in autopsy-brain biopsies from AIDS victims in micronodular areas characterized by an increased cellularity and the presence of multinuclear giant cells. (Abstract shortened by UMI.)
Keywords: Acquired Immunodeficiency Syndrome/*PATHOLOGY Antibodies, Monoclonal/DIAGNOSTIC USE AIDS-Related Complex/*PATHOLOGY B-Lymphocytes/PATHOLOGY Brain/PATHOLOGY Human HIV/IMMUNOLOGY HIV Antigens/ANALYSIS *Immunoenzyme Techniques Lymph Nodes/PATHOLOGY Lymphoma, Non-Hodgkin's/*PATHOLOGY THESIS 891230
M89C0818
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Important note: Information in this article was accurate in 1989. The state of the art may have changed since the publication date.
