High affinity interleukin 2 receptors in HTLV-1-infected T cells can mediate signals for gene expression. NLM AIDSLINE Important note: Information in this article was accurate in 1989. The state of the art may have changed since the publication date.

Click here to return to AIDSLINE main menu
DonateNow
Print this Article


High affinity interleukin 2 receptors in HTLV-1-infected T cells can mediate signals for gene expression.

Virus Genes. 1987 Nov;1(1):35-47. Unique Identifier : AIDSLINE MED/89224165
Sakitani M; Nakamura M; Fujii M; Sugamura K; Hinuma Y; Institute for Virus Research, Kyoto University, Japan.

Abstract: The expression of transcripts of the c-myb and c-myc protooncogenes and the interleukin 2 receptor (IL-2R) gene in human T cells infected with human T-cell leukemia virus type 1 (HTLV-1) after exposure to interleukin 2 (IL-2) were examined. Infection with HTLV-1 is known to be associated with constitutive expression of IL-2R, although infected cells do not require IL-2 for growth. Northern blot analysis showed that expression of the mRNAs of the c-myb, c-myc, and IL-2R genes were markedly increased by addition of IL-2 into the cultures, indicating that IL-2R transduced signals for gene expression in these cells as in normal T cells. Studies on distinct HTLV-1-infected T cell clones that differed in numbers of high-affinity IL-2R, showed that the extents of increase in mRNA expression by IL-2 were correlated with the number of high-affinity IL-2R. This correlation was confirmed by demonstration that the levels of mRNA expression were proportional to the numbers of IL-2-bound high-affinity but not low-affinity receptors. Thus, the signals induced by IL-2 for gene expression may be through high-affinity IL-2R.
Keywords: Blotting, Northern Cell Line *Gene Expression Regulation Human HTLV-I/*GENETICS Interleukin-2/METABOLISM Kinetics Receptors, Interleukin-2/GENETICS/*METABOLISM Recombinant Proteins/BIOSYNTHESIS RNA, Messenger/ANALYSIS/METABOLISM *Signal Transduction Support, Non-U.S. Gov't T-Lymphocytes/*METABOLISM/MICROBIOLOGY JOURNAL ARTICLE

KWDblotting,northerncelllineKWDgeneexpressionregulationhumanhtlv-i/KWDgeneticsinterleukin-2/metabolismkineticsreceptors,interleukin-2/genetics/KWDmetabolismrecombinantproteins/biosynthesisrna,messenger/analysis/metabolismKWDsignaltransductionsupport,non-uKWDsKWDgov'tt-lymphocytes/KWDmetabolism/microbiologyjournalarticle
890830
M8980813

Copyright © 1989 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1989. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1989. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .