Site-directed ELISA identifies a highly antigenic region of the simian immunodeficiency virus transmembrane glycoprotein. NLM AIDSLINE Important note: Information in this article was accurate in 1988. The state of the art may have changed since the publication date.

Click here to return to AIDSLINE main menu
DonateNow
Print this Article


Site-directed ELISA identifies a highly antigenic region of the simian immunodeficiency virus transmembrane glycoprotein.

AIDS Res Hum Retroviruses. 1988 Jun;4(3):159-64. Unique Identifier : AIDSLINE MED/88281277
Johnson PR; Parks DE; Norrby E; Lerner RA; Purcell RH; Chanock RM; Laboratory of Infectious Diseases, National Institute of Allergy; and Infectious Diseases, Bethesda, MD.

Abstract: The transmembrane glycoprotein (gp32) of the simian immunodeficiency virus (SIV) contains a highly antigenic region that includes amino acid residues 606-628. A synthetic peptide representing this region was highly immunoreactive with sera from SIV-infected primates in a site-directed enzyme-linked immunosorbent assay (ELISA). This reactivity extended across four primate species from three genera and identified infection with at least two distinct isolates of SIV. This site-directed ELISA represents a simple, accessible method with broad specificity for screening large numbers of primates for antibodies against SIV.
Keywords: Amino Acid Sequence Animal Antigens, Viral/*ANALYSIS Cercopithecus aethiops Enzyme-Linked Immunosorbent Assay Glycoproteins/*ANALYSIS/IMMUNOLOGY Macaca mulatta Monkey Diseases/*MICROBIOLOGY Retroviridae/*IMMUNOLOGY Viral Envelope Proteins/*ANALYSIS JOURNAL ARTICLE

KWDaminoacidsequenceanimalantigens,viral/KWDanalysiscercopithecusaethiopsenzyme-linkedimmunosorbentassayglycoproteins/KWDanalysis/immunologymacacamulattamonkeydiseases/KWDmicrobiologyretroviridae/KWDimmunologyviralenvelopeproteins/KWDanalysisjournalarticle
881130
M88B0568

Copyright © 1988 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1988. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1988. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .