Interactions of cellular proteins involved in the transcriptional regulation of the human immunodeficiency virus. NLM AIDSLINE Important note: Information in this article was accurate in 1988. The state of the art may have changed since the publication date.

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Interactions of cellular proteins involved in the transcriptional regulation of the human immunodeficiency virus.

EMBO J. 1987 Dec 1;6(12):3761-70. Unique Identifier : AIDSLINE MED/88111565
Garcia JA; Wu FK; Mitsuyasu R; Gaynor RB; Department of Medicine, UCLA School of Medicine 90024.

Abstract: The human immunodeficiency virus (HIV) is a human retrovirus which is the etiologic agent of the acquired immunodeficiency syndrome. To study the cellular factors involved in the transcriptional regulation of this virus, we performed DNase I footprinting of the viral LTR using partially purified HeLa cell extracts. Five regions of the viral LTR appear critical for DNA binding of cellular proteins. These include the negative regulatory, enhancer, SP1, TATA and untranslated regions. Deletion mutagenesis of these binding domains has significant effects on the basal level of transcription and the ability to be induced by the viral tat protein. Mutations of either the negative regulatory or untranslated regions affect factor binding to the enhancer region. In addition, oligonucleotides complementary to several of the binding domains specifically compete for factor binding. These results suggest that interactions between several distinct cellular proteins are required for HIV transcriptional regulation.
Keywords: Chromosome Deletion *Gene Expression Regulation *Genes, Structural *Genes, Viral Hela Cells/METABOLISM Human HIV/*GENETICS Mutation Neoplasm Proteins/*PHYSIOLOGY Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Transcription Factors/*GENETICS *Transcription, Genetic Transfection JOURNAL ARTICLE

KWDchromosomedeletionKWDgeneexpressionregulationKWDgenes,structuralKWDgenes,viralhelacells/metabolismhumanhiv/KWDgeneticsmutationneoplasmproteins/KWDphysiologysupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDtranscriptionfactors/KWDgeneticsKWDtranscription,genetictransfectionjournalarticle
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M8850455

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