Transmission and genetic shift of human immunodeficiency virus (HIV) in vivo. NLM AIDSLINE Important note: Information in this article was accurate in 1988. The state of the art may have changed since the publication date.

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Transmission and genetic shift of human immunodeficiency virus (HIV) in vivo.

Mol Biol Med. 1987 Dec;4(6):385-96. Unique Identifier : AIDSLINE MED/88142445
Yoshiyama H; Kobayashi N; Matsui T; Nakashima H; Kajii T; Yamato K; Kotani S; Miyoshi I; Yamamoto N; Department of Virology and Parasitology, Yamaguchi University; School of Medicine, Japan.

Abstract: HIVYU-6 and HIVYU-7 were isolated from an acquired immune deficiency syndrome patient (MK) and his asymptomatic sexual partner (MM), respectively. YU-6 readily infected not only peripheral lymphocytes from normal individuals but also human T-cell lines such as H9, HUT-78, MOLT-4 and MT-4; YU-7, on the other hand, could not infect H9 and MT-4 cells. Furthermore, although autologous serum failed to neutralize YU-6, it was neutralized by the heterologous serum from the partner. Restriction endonuclease analysis of YU-6 demonstrated that it was a mixture of viruses. We have isolated two clones from YU-6 (YU-6-a and YU-6-b) by a plaque assay method and showed that YU-6-a had one more KpnI site than YU-6-b. It was also evident that YU-7 derived from YU-6-a, but had already shifted genetically from YU-6-a. Transmission of human immunodeficiency virus through heterosexual contact and a possible genetic shift of YU-6-a, b and YU-7 from a common progenitor virus in vivo is discussed.
Keywords: Acquired Immunodeficiency Syndrome/MICROBIOLOGY/*TRANSMISSION Adult Antibodies, Viral/IMMUNOLOGY Cell Line Comparative Study Female Hemophilia/COMPLICATIONS Human HIV/*GENETICS/IMMUNOLOGY/ISOLATION & PURIF Male Mutation Neutralization Tests Sex Behavior Support, Non-U.S. Gov't T-Lymphocytes/MICROBIOLOGY Virus Cultivation JOURNAL ARTICLE

KWDacquiredimmunodeficiencysyndrome/microbiology/KWDtransmissionadultantibodies,viral/immunologycelllinecomparativestudyfemalehemophilia/complicationshumanhiv/KWDgenetics/immunology/isolation&purifmalemutationneutralizationtestssexbehaviorsupport,non-uKWDsKWDgov'tt-lymphocytes/microbiologyviruscultivationjournalarticle
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M8860429

Copyright © 1988 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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