A glycoprotein inhibitor of in vitro granulopoiesis associated with AIDS. NLM AIDSLINE Important note: Information in this article was accurate in 1988. The state of the art may have changed since the publication date.

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A glycoprotein inhibitor of in vitro granulopoiesis associated with AIDS.

Blood. 1987 Nov;70(5):1267-72. Unique Identifier : AIDSLINE MED/88025542
Leiderman IZ; Greenberg ML; Adelsberg BR; Siegal FP; Division of Clinical Immunology, Mount Sinai School of Medicine; (CUNY).

Abstract: Patients infected with the human immunodeficiency virus (HIV) often present with neutropenia. To elucidate the mechanism(s) of this HIV-related neutropenia, we assessed the proliferative capacity of the granulocyte-macrophage progenitor cell (CFU-GM) from the bone marrow (BM) of 78 patients within the AIDS spectrum manifesting symptoms or signs related to HIV infection. Of these, 70 had a significant deficit in the growth of this committed progenitor when compared with normal controls (P less than .01). Further analysis revealed that the nucleated bone marrow cells from AIDS and AIDS-related complex (ARC) patients inhibited the growth of CFU-GMs from normal individuals when cocultured in agar (P less than .001). Control CFU-GMs were also inhibited when they were cultured over feeder layers containing patients' BM cells (P less than .001). Conditioned media obtained from the liquid culture of patients' BM cells did not inhibit normal control CFU-GM growth to a degree different from that of the cells themselves (P greater than .4). Analysis of these conditioned media by polyacrylamide gel electrophoresis (PAGE) revealed a unique glycoprotein (gp) with a mol wt of 84 kd. Further studies revealed that this gp possessed the inhibitory activity. These data suggest that this gp may be an important factor in HIV-related neutropenia. The presence of gp84 was independent of drugs administered to the patients.
Keywords: Acquired Immunodeficiency Syndrome/*PHYSIOPATHOLOGY Bone Marrow/PATHOLOGY Cells, Cultured Colony-Forming Units Assay Female Glycoproteins/ISOLATION & PURIF/*PHYSIOLOGY Granulocytes/*CYTOLOGY *Hematopoiesis Human Leukopenia/ETIOLOGY Male Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE

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M8820356

Copyright © 1988 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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