Changes induced in antibodies by isolation methods.

Important note: Information in this article was accurate in 1988. The state of the art may have changed since the publication date.

Changes induced in antibodies by isolation methods.

Biotechnol Appl Biochem. 1988 Feb;10(1):63-71. Unique Identifier : AIDSLINE MED/88309401
McCue JP; Sasagawa PK; Hein RH; Department of Chemistry & Physics, Salem State College,; Massachusetts 01970.

Abstract: IgG was isolated from plasma at low temperatures using the Cohn fractionation method, and then processed to three different products in order to evaluate how chemical and physical manipulations affect antibodies. Evaluation of the antibodies was done by measuring their ability to bind human immunodeficiency virus, cytomegalovirus, Herpes simplex virus, and rubella virus. In addition, the IgG products were compared by crossed immunoelectrophoresis, and circular dichroism and ultra violet spectroscopy. It was found that exposure of purified IgG to physiological pH altered the molecular conformation of IgG and induced various degrees of irreversible loss in antibody binding to viruses. These observations indicate that more efficacious antibodies may be obtained for clinical use if isolation at their isoelectric point is avoided.

Keywords: Comparative Study Freeze Drying/METHODS Human Hydrogen-Ion Concentration IgG/IMMUNOLOGY/*ISOLATION & PURIF Precipitation/METHODS Ultrafiltration/METHODS Viruses/IMMUNOLOGY JOURNAL ARTICLE

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