Two distinct sequence elements mediate retroviral gene expression in embryonal carcinoma cells. NLM AIDSLINE Important note: Information in this article was accurate in 1987. The state of the art may have changed since the publication date.

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Two distinct sequence elements mediate retroviral gene expression in embryonal carcinoma cells.

J Virol. 1987 Sep;61(9):2742-6. Unique Identifier : AIDSLINE GENBANK/M17289
Weiher H; Barklis E; Ostertag W; Jaenisch R

Abstract: Moloney murine leukemia virus (M-MuLV) and M-MuLV-derived retroviral vectors are not expressed in early mouse embryos or in embryonal carcinoma cells. M-MuLV-derived mutants or M-MuLV-related variants which transduce the neomycin phosphotransferase gene can, however, induce drug resistance in embryonal carcinoma cells with high efficiency. In this study we investigated the sequences critical for retroviral gene expression in two different embryonal carcinoma cell lines, F9 and PCC4. We show that two synergistically acting sequence elements mediate expression in embryonal carcinoma cells. One of these is located within the U3 region of the viral long terminal repeat, and the second one is in the 5' untranslated region of the retrovirus. The latter element, characterized by a single point mutation, affects the level of stable RNA in infected cells, suggesting a regulatory mechanism similar to that of human immunodeficiency virus in human T cells.
Keywords: Animal Base Sequence Cells, Cultured *Gene Expression Regulation *Genes, Viral Mice Mutation Repetitive Sequences, Nucleic Acid Retroviridae/*GENETICS RNA, Viral/ANALYSIS Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Transduction, Genetic Tumor Stem Cells/*MICROBIOLOGY JOURNAL ARTICLE

KWDanimalbasesequencecells,culturedKWDgeneexpressionregulationKWDgenes,viralmicemutationrepetitivesequences,nucleicacidretroviridae/KWDgeneticsrna,viral/analysissupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDtransduction,genetictumorstemcells/KWDmicrobiologyjournalarticle
871130
M87B0338

Copyright © 1987 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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