Abundant synthesis of functional human T-cell leukemia virus type I p40x protein in eucaryotic cells by using a baculovirus expression vector. NLM AIDSLINE Important note: Information in this article was accurate in 1987. The state of the art may have changed since the publication date.

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Abundant synthesis of functional human T-cell leukemia virus type I p40x protein in eucaryotic cells by using a baculovirus expression vector.

J Virol. 1987 Mar;61(3):708-13. Unique Identifier : AIDSLINE MED/87112978
Jeang KT; Giam CZ; Nerenberg M; Khoury G

Abstract: The human T-cell leukemia virus type I (HTLV-I) p40x protein is a 40-kilodalton polypeptide encoded in the 3'-terminal region of the virus. This protein is responsible for positive transcriptional trans-activation of promoter elements located within the HTLV-I long terminal repeat. We introduced the protein-coding region of HTLV-I p40x into the genome of the baculovirus Autographa californica nuclear polyhedrosis virus. After infection of the insect Spodoptera frugiperda (SF9) cell line, this recombinant strain of baculovirus produced approximately 200 mg of intact p40x protein per 2.5 X 10(8) cells. The protein was biologically active in trans-activation of an HTLV-I long terminal repeat-human beta-globin construct. Biochemical analyses of the protein suggest that the p40x polypeptide underwent posttranslational modification in these eucaryotic SF9 cells.
Keywords: Biological Assay Cell Line Cloning, Molecular Gene Expression Regulation Genetic Vectors HTLV-BLV Viruses/*GENETICS Insect Viruses/*GENETICS Insects Isoelectric Point Molecular Weight Protein Processing, Post-Translational Support, Non-U.S. Gov't Transcription, Genetic Viral Proteins/*GENETICS JOURNAL ARTICLE

KWDbiologicalassaycelllinecloning,moleculargeneexpressionregulationgeneticvectorshtlv-blvviruses/KWDgeneticsinsectviruses/KWDgeneticsinsectsisoelectricpointmolecularweightproteinprocessing,post-translationalsupport,non-uKWDsKWDgov'ttranscription,geneticviralproteins/KWDgeneticsjournalarticle
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M8750080

Copyright © 1987 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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