Structure and antigenicity of the specific oligosaccharide hapten from the glycopeptidolipid antigen of Mycobacterium avium serotype 4, the dominant Mycobacterium isolated from patients with acquired immune deficiency syndrome.

Important note: Information in this article was accurate in 1987. The state of the art may have changed since the publication date.

J Biol Chem. 1987 Feb 25;262(6):2630-5. Unique Identifier : AIDSLINE MED/87137509
McNeil M; Tsang AY; Brennan PJ

Abstract: A large number of patients with acquired immune deficiency syndrome develop disseminated infections due to member serotypes of the Mycobacterium avium complex. Seroagglutination on 181 such isolates followed by enzyme-linked immunosorbent assay and thin layer chromatography of the type-specific glycopeptidolipid (GPL) antigens demonstrated that the majority of serotypes were M. avium serotype 4. The specific GPL of serotype 4 was isolated in both the native, acetylated, and the deacetylated forms and its oligosaccharide hapten released as the oligosaccharide alditol by reductive beta-elimination. A comprehensive structural analytical approach developed for more complex carbohydrates was applied to the oligosaccharide alditol in order to reveal glycosyl and glycosyl-linkage composition, sequence arrangements, ring forms, and enantiomeric and anomeric configurations. The structure of the triglycosyl alditol was established as, 4-O-Me-L-Rhap-(alpha 1----4)-2-O-Me-L-Fucp-(alpha 1----3)-L-Rhap- (alpha 1----2)-6-deoxytalitol, in which the nonreducing-end disaccharide unit is unique to serotype 4. The native GPL antigen is diacetylated, presumably at other than the terminal disaccharide, since the antigenicity of both the acetylated and deacetylated antigens are comparable. The structure of the epitope of the type-specific antigen of serotype 4 will serve as the basis for synthetic antigen probes and the target for the monoclonal antibodies required to trace the origins in the environment of the infectious agent and study the epidemiology of human infections.

Keywords: Acquired Immunodeficiency Syndrome/*IMMUNOLOGY/MICROBIOLOGY Antigenic Determinants/*ANALYSIS Antigens, Bacterial/ANALYSIS Chromatography, Thin Layer Enzyme-Linked Immunosorbent Assay Haptens/*IMMUNOLOGY Human Mass Fragmentography Mycobacterium avium/*IMMUNOLOGY Oligosaccharides/*IMMUNOLOGY Serotyping Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE

870630
M8760382

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