HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNA. NLM AIDSLINE Important note: Information in this article was accurate in 1987. The state of the art may have changed since the publication date.

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HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNA.

Cell. 1986 Sep 12;46(6):807-17. Unique Identifier : AIDSLINE GENBANK/K03455
Feinberg MB; Jarrett RF; Aldovini A; Gallo RC; Wong-Staal F

Abstract: The African green monkey nonlymphoid cell line cos-1 produces infectious HTLV-III virus following transfection with biologically active molecular clones of HTLV-III. Transfected cos-1 cells produce large amounts of viral RNA and protein. We have used this rapid transfection system to study the regulatory functions and synthetic capacity of the HTLV-III genome, as well as mutants derived from it. Analysis of transfected lymphoid and nonlymphoid cell lines suggests that tat-III-mediated trans-activation of viral gene expression is operative predominantly, if not exclusively, at a posttranscriptional level. We have also identified an additional HTLV-III-encoded gene that controls viral gene expression through regulation of the relative proportions of the various viral RNA transcripts and is required for viral replication.
Keywords: Animal Cell Line Gene Expression Regulation Genes, Viral HIV/*GENETICS Retroviridae Proteins/*GENETICS *RNA Splicing RNA, Viral/*GENETICS Transcription Factors/GENETICS Translation, Genetic Virus Replication JOURNAL ARTICLE

KWDanimalcelllinegeneexpressionregulationgenes,viralhiv/KWDgeneticsretroviridaeproteins/KWDgeneticsKWDrnasplicingrna,viral/KWDgeneticstranscriptionfactors/geneticstranslation,geneticvirusreplicationjournalarticle
870130
M8710183

Copyright © 1987 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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