Sensitive assay for neutralizing antibodies against AIDS-related viruses (HTLV-III/LAV). NLM AIDSLINE Important note: Information in this article was accurate in 1987. The state of the art may have changed since the publication date.

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Sensitive assay for neutralizing antibodies against AIDS-related viruses (HTLV-III/LAV).

J Immunol Methods. 1986 Sep 27;92(2):177-81. Unique Identifier : AIDSLINE MED/87009993
Harada S; Purtilo DT; Koyanagi Y; Sonnabend J; Yamamoto N

Abstract: A sensitive assay for neutralizing antibodies (NA) against AIDS-related viruses (HTLV-III and LAV) was developed, using human T-cell lymphotropic virus type-I (HTLV-I)-bearing and HTLV-III-susceptible MT-4 cells. NA to HTLV-III in 21 patients with acquired immune deficiency syndrome (AIDS), 10 individuals with AIDS-related complex (ARC), 20 healthy male homosexuals, and 10 healthy male controls were titrated. Antibodies to HTLV-III were also detected by indirect immunofluorescence (IF). The assay was sensitive up to a dilution of 1:10 000. Sera from patients with AIDS showed a geometric mean titer (GMT) of NA of 1:475, whereas much higher GMTs (1:1318 and 1:1009) were observed in patients with ARC and healthy male homosexuals, respectively. Moreover, titers of NA significantly correlated with the levels of anti-HTLV-III antibodies detected by IF.
Keywords: Antibodies, Viral/*ANALYSIS Antigen-Antibody Reactions Antigens, Viral/*IMMUNOLOGY Cell Line Human HIV/*IMMUNOLOGY Male Methods Neutralization Tests Support, Non-U.S. Gov't JOURNAL ARTICLE

KWDantibodies,viral/KWDanalysisantigen-antibodyreactionsantigens,viral/KWDimmunologycelllinehumanhiv/KWDimmunologymalemethodsneutralizationtestssupport,non-uKWDsKWDgov'tjournalarticle
870130
M8710180

Copyright © 1987 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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