Important note: Information in this article was accurate in 1987. The state of the art may have changed since the publication date.
HUMAN B CELL POPULATIONS DEFINED BY THE B1 AND B2 ANTIGENS
Leukocyte Typing II; 2:413-28 1986. Unique Identifier : AIDSLINE ICDB/87626871 Anderson KC; Boyd AW; Fisher DC; Daley JF; Schlossman SF; Nadler LM; Div. of Tumor Immunology, Dana-Farber Cancer Inst., Dept. of; Medicine, Harvard Medical Sch., Boston, MA 02115
Abstract:
Dual-fluorochrome staining and flow cytometry were used to identify two B cell populations in normal peripheral blood, lymph node, spleen, and tonsil, and in lymph node tissue from patients (pts) with acquired immunodeficiency syndrome (AIDS, 8) or AIDS-related complex (ARC, 9). The majority of B cells identified coexpressed both B1 and B2 (B1+,B2+), whereas a minority (5-15%) expressed only B1 (B1+,B2-). A small number of cells appeared to exhibit a B1-,B2+ phenotype, but could not be purified or analyzed in significant numbers. Virtually all B1+B2+ cells expressed IgM and IgD but lacked IgG and the plasma cell antigens PCA-1 and PC-1, whereas the B1+B2- cells more frequently expressed IgG, PCA-1, and PC-1. Both populations were noncycling and were composed of similar percentages of large and small cells. The B1+B2+ cells proliferate in response to anti-u, or to anti-u + phytohemagglutinin-stimulated, leukocyte-conditioned medium (PHA-LCM), but not to PHA-LCM alone. They require both T cells and pokeweed mitogen (PWM) to produce immunoglobulin. In contrast, B1+B2- cells do not significantly proliferate to anti-u, PHA-LCM, or anti-u + PHA-LCM. They produce Ig in response to T cells alone without PWM. These phenotypic and functional observations provide preliminary evidence that these populations are distinct and that the B1+B2+ cell may be a 'resting' cell whereas the B1+B2- cell appears to be 'differentiated.' In lymph nodes from pts with AIDS and ARC, who have elevated numbers of cells spontaneously secreting immunoglobulins and decreased B cell proliferative responses to T cell-independent B cell mitogens, there was a marked shift to predominantly B1+B2- cells. This observation supports the view that the B1+B2- phenotype may identify a more differentiated B cell phenotype and further suggests that analysis of the B cells may yield additional parameters that reflect disease activity (ie, the number of B1+B2- B cells). (38 Refs)
Keywords: Acquired Immunodeficiency Syndrome/IMMUNOLOGY Antibodies, Monoclonal/*IMMUNOLOGY Antibody Specificity Antigens, Surface/*IMMUNOLOGY B-Lymphocytes/*IMMUNOLOGY Cell Division Flow Cytometry Human Immunoglobulins/METABOLISM Leukemia, Lymphocytic/IMMUNOLOGY Lymph Nodes/IMMUNOLOGY Lymphoma/IMMUNOLOGY Phosphoproteins/IMMUNOLOGY Spleen/IMMUNOLOGY T-Lymphocytes, Helper-Inducer/IMMUNOLOGY Tonsil/IMMUNOLOGY MEETING PAPER
AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.
Important note: Information in this article was accurate in 1987. The state of the art may have changed since the publication date.
