A rapid, no-wash technic for immunophenotypic analysis by flow cytometry. NLM AIDSLINE Important note: Information in this article was accurate in 1987. The state of the art may have changed since the publication date.

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A rapid, no-wash technic for immunophenotypic analysis by flow cytometry.

Am J Clin Pathol. 1986 Nov;86(5):600-7. Unique Identifier : AIDSLINE MED/87045937
Caldwell CW; Taylor HM

Abstract: With the advent of fluorochrome-labeled monoclonal antibodies, it has now become possible to eliminate the removal of unbound antibodies during immunophenotypic analysis of lymphoid cells by flow cytometry. Suspensions of mononuclear cells from healthy controls and fluoresceinated monoclonal antibodies may be introduced directly into the flow cytometer. The percentage of nonwashed antibody-positive cells from healthy controls as compared with the conventional technic of cell washing shows a good correlation. However, the correlation is not as good when the lymphocytes from certain classes of diseased patients are analyzed. It appears that excessive washing in fact produces erroneous results for certain markers with cells from diseased patients. The no-wash technic is also applicable to various combinations of dual-fluorescence monoclonal antibody staining. This technic substantially reduces the processing time and, more importantly, eliminates some of the cell losses and artifacts induced during the process of cell washing. The shortened processing time has allowed rapid responsiveness in certain urgent clinical situations.
Keywords: Acquired Immunodeficiency Syndrome/BLOOD Adult Antibodies, Monoclonal/DIAGNOSTIC USE Autoimmune Diseases/BLOOD Female Flow Cytometry Fluoresceins/DIAGNOSTIC USE Human Infection/BLOOD Leukemia/BLOOD Lymphocytes/*IMMUNOLOGY Male JOURNAL ARTICLE

KWDacquiredimmunodeficiencysyndrome/bloodadultantibodies,monoclonal/diagnosticuseautoimmunediseases/bloodfemaleflowcytometryfluoresceins/diagnosticusehumaninfection/bloodleukemia/bloodlymphocytes/KWDimmunologymalejournalarticle
870228
M8720193

Copyright © 1987 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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