Important note: Information in this article was accurate in 1987. The state of the art may have changed since the publication date.
A MOLECULAR CHARACTERIZATION OF THE GENOME OF MASON-PFIZER MONKEY VIRUS
Diss Abstr Int (Sci); 47(1):74 1986. Unique Identifier : AIDSLINE ICDB/87638056 Barker CS; University of Alabama at Birmingham
Abstract:
Mason-Pfizer monkey virus (M-PMV) is the prototype of the D-type retroviruses. The DNA provirus of M-PMV has been shown to be 8.1 kilobase pairs in length, and has long terminal repeats that are 350 base pairs in length. DNA fragments representing the LTR-gag and env regions of the provirus have been molecularly cloned from an acutely infected human cell line. A detailed restriction map of the provirus was developed and oriented relative to viral transcription. Fourteen copies of integrated M-PMV proviruses were molecularly cloned from a chronically infected human cell line. Restriction analysis revealed that all clones were apparently full length and confirmed the previously derived restriction map. The clones were assayed for biological activity by transfection into three different human cell lines. Thirteen of 14 clones produced infectious virus, and one of these clones produced higher titers of virus than all of the other clones, suggesting that it contained a mutation in some stage of virus replication. The virus produced following transfection of these clones was compared to uncloned M-PMV and no differences in the virion protein profile were detected. Analysis of the rescued virus revealed that the molecularly cloned M-PMV was capable of inducing cell fusion. M-PMV was compared to 12 other molecularly cloned retroviruses. Two other D-type retroviruses were found to be very closely related to M-PMV, simian AIDS associated virus (SAIDS-D) and squirrel monkey retrovirus (SMRV). However the SAIDS-D virus was much more closely related to M-PMV than was SMRV. M-PMV was also found to be distantly related to mouse mammary tumor virus, a type-B retrovirus. Homology was also detected between the pol gene of M-PMV and a new class of human endogenous sequences. The chromosomal DNA from a variety of primates was examined for the presence of sequences related to subgenomic clones of the M-PMV provirus. It is concluded that the D-type and B-type viruses are more closely related than previously suspected and can be placed in a larger viral grouping. In addition, multiple copies of endogenous sequences related to M-PMV have been detected, suggesting that these sequences may serve as a pool for generation of novel primate viruses related to M-PMV. (Abstract shortened with permission of author.)
Keywords: Cell Line *Cloning, Molecular DNA, Viral/*GENETICS Human Polymorphism, Restriction Fragment Length Retroviridae/*GENETICS Sequence Homology, Nucleic Acid *Transcription, Genetic THESIS
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Important note: Information in this article was accurate in 1987. The state of the art may have changed since the publication date.
