Lymphocyte phenotyping by fluorescence microscopy and flow cytometry: results in homosexual men and heterosexual controls. NLM AIDSLINE Important note: Information in this article was accurate in 1986. The state of the art may have changed since the publication date.

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Lymphocyte phenotyping by fluorescence microscopy and flow cytometry: results in homosexual men and heterosexual controls.

AIDS Res. 1983-84;1(2):127-34. Unique Identifier : AIDSLINE MED/86242449
Newman JT; Nicodemus DS; Ordonez GA; Stone MJ

Abstract: Absolute numbers of peripheral blood T3, T4 and T8 positive cells from 15 homosexual men and concurrent controls were determined by fluorescence microscopy (FM) and by a fluorescence-activated cell sorter (FACS). A significant difference in the number of positive cells was observed between FM and FACS in the control group for all three monoclonal antibodies and for the T4/T8 ratio. FACS methodology yielded a lower number of T4 positive cells and a lower T4/T8 ratio in the homosexual subjects. Two homosexual men had normal T4/T8 ratios by FM but were found to have low ratios by FACS. The reasons for the disparate results obtained by the two methods are unclear, but such findings are important to bear in mind when evaluating male homosexuals for immunologic abnormalities.
Keywords: Acquired Immunodeficiency Syndrome/*IMMUNOLOGY Adult Aged Cell Separation Comparative Study Flow Cytometry *Homosexuality Human Male Microscopy, Fluorescence Middle Age Support, Non-U.S. Gov't T-Lymphocytes/*CLASSIFICATION JOURNAL ARTICLE

KWDacquiredimmunodeficiencysyndrome/KWDimmunologyadultagedcellseparationcomparativestudyflowcytometryKWDhomosexualityhumanmalemicroscopy,fluorescencemiddleagesupport,non-uKWDsKWDgov'tt-lymphocytes/KWDclassificationjournalarticle
861030
M86A0314

Copyright © 1986 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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