Effect of heat treatment on results for biochemical analysis of plasma and serum. NLM AIDSLINE Important note: Information in this article was accurate in 1986. The state of the art may have changed since the publication date.

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Effect of heat treatment on results for biochemical analysis of plasma and serum.

Clin Chem. 1985 Dec;31(12):2028-30. Unique Identifier : AIDSLINE MED/86053960
Houssein I; Wilcox H; Barron J

Abstract: Incubation of serum and plasma samples at 56 degrees C for 30 min inactivates the HTLV-III virus. We assessed the effect of this procedure on results of routine biochemical tests by dividing samples of serum and plasma into two, heat-treating one while the other remained at room temperature. Samples were then analyzed in an SMA 16/60, an Astra-8, an Analox glucose analyzer, a Cobas Bio centrifugal analyzer, and manually for salicylate and acetaminophen (paracetamol). Most of the differences produced by heat treatment were not clinically significant, although heated samples proved unsuitable for use in assay of some commonly measured enzymes. Serum evidently is preferable to plasma for this procedure, and heat-treated serum samples can validly be used for most routine analyses. Thus this procedure makes safer the analysis of samples from patients with suspected or proven acquired immune deficiency syndrome (AIDS).
Keywords: Acquired Immunodeficiency Syndrome/PREVENTION & CONTROL Autoanalysis Blood Chemical Analysis/*METHODS *Heat Human Laboratory Infection/*PREVENTION & CONTROL Plasma/ANALYSIS JOURNAL ARTICLE

KWDacquiredimmunodeficiencysyndrome/prevention&controlautoanalysisbloodchemicalanalysis/KWDmethodsKWDheathumanlaboratoryinfection/KWDprevention&controlplasma/analysisjournalarticle
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M8630185

Copyright © 1986 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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