Enzyme immunoassay detection of immunoglobulin M and G antibodies to Cryptosporidium in immunocompetent and immunocompromised persons. NLM AIDSLINE Important note: Information in this article was accurate in 1986. The state of the art may have changed since the publication date.

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Enzyme immunoassay detection of immunoglobulin M and G antibodies to Cryptosporidium in immunocompetent and immunocompromised persons.

J Infect Dis. 1986 Mar;153(3):570-8. Unique Identifier : AIDSLINE MED/86141988
Ungar BL; Soave R; Fayer R; Nash TE

Abstract: Cryptosporidium is a parasite of the human gastrointestinal tract and has a worldwide distribution. We developed a sensitive and reproducible enzyme immunoassay for detection of serum IgG or IgM to Cryptosporidium. For IgG, 13 of 15 patients with cryptosporidiosis and 26 of 26 patients with cryptosporidiosis and AIDS were positive, whereas 57 of 60 presumably uninfected individuals were negative. All three IgG-positive presumably uninfected individuals had been potentially exposed. Sensitivity and specificity of this assay was 95%. Patients without AIDS showed an early rise and fall of IgM and later elevation of IgG; some patients with AIDS produced IgM, and all produced IgG. Sera from 9 (20.9%) of 44 Ecuadorian children with diarrhea were positive for both IgM and IgG antibodies; 106 sera from persons with other parasitic illnesses showed a normal distribution for IgG antibody. These ELISA data show that patients without and with AIDS have serum antibody response to Cryptosporidium and suggest that exposure to or infection with Cryptosporidium is common.
Keywords: Acquired Immunodeficiency Syndrome/COMPLICATIONS Animal Antibody Formation Child Coccidia/*IMMUNOLOGY Cryptosporidiosis/COMPLICATIONS/DIAGNOSIS Cryptosporidium/*IMMUNOLOGY Diarrhea/COMPLICATIONS/PARASITOLOGY Enzyme-Linked Immunosorbent Assay Feces/PARASITOLOGY Human IgG/*ANALYSIS IgM/*ANALYSIS *Immunosuppression JOURNAL ARTICLE

KWDacquiredimmunodeficiencysyndrome/complicationsanimalantibodyformationchildcoccidia/KWDimmunologycryptosporidiosis/complications/diagnosiscryptosporidium/KWDimmunologydiarrhea/complications/parasitologyenzyme-linkedimmunosorbentassayfeces/parasitologyhumanigg/KWDanalysisigm/KWDanalysisKWDimmunosuppressionjournalarticle
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M8660205

Copyright © 1986 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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