Nucleotide sequence of the protease-coding region in an infectious DNA of simian retrovirus (STLV) of the HTLV-I family. NLM AIDSLINE Important note: Information in this article was accurate in 1986. The state of the art may have changed since the publication date.

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Nucleotide sequence of the protease-coding region in an infectious DNA of simian retrovirus (STLV) of the HTLV-I family.

Virology. 1986 Apr 15;150(1):187-95. Unique Identifier : AIDSLINE GENBANK/J02029
Inoue J; Watanabe T; Sato M; Oda A; Toyoshima K; Yoshida M; Seiki M

Abstract: A provirus clone of simian T-cell leukemia virus isolated from a pigtailed monkey (PT-STLV), which is 90% homologous to HTLV-I, was shown to be biologically active in transfection assay. In transfected cells, gp61env, Pr55gag, and the mature gag proteins p24, p21, and p15 were detected, and type C particles were produced. The virus could be transmitted from the transfectants to recipient cells by cocultivation. In this biologically active provirus clone, a coding frame, possibly for protease, was identified between the gag and pol genes. The corrected sequence of the protease region of HTLV-I was also found to have a single open reading frame overlapping the gag and pol genes, although it has an amber codon in the middle of the frame. Thus, a single coding frame, which is different from those of gag and pol, is common to proteases of the HTLV family including HTLV-I.
Keywords: Animal Base Sequence Cloning, Molecular Comparative Study Genes, Structural *Genes, Viral Macaca/*MICROBIOLOGY Macaca nemestrina/*MICROBIOLOGY Peptide Hydrolases/*GENETICS Retroviridae/ENZYMOLOGY/*GENETICS/IMMUNOLOGY Retroviridae Proteins/IMMUNOLOGY Sequence Homology, Nucleic Acid Support, Non-U.S. Gov't Viral Envelope Proteins/IMMUNOLOGY Virus Replication JOURNAL ARTICLE

KWDanimalbasesequencecloning,molecularcomparativestudygenes,structuralKWDgenes,viralmacaca/KWDmicrobiologymacacanemestrina/KWDmicrobiologypeptidehydrolases/KWDgeneticsretroviridae/enzymology/KWDgenetics/immunologyretroviridaeproteins/immunologysequencehomology,nucleicacidsupport,non-uKWDsKWDgov'tviralenvelopeproteins/immunologyvirusreplicationjournalarticle
860630
M8660165

Copyright © 1986 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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