[Antiglobulin test using an immunoenzyme method] NLM AIDSLINE Important note: Information in this article was accurate in 1986. The state of the art may have changed since the publication date.

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[Antiglobulin test using an immunoenzyme method]

Quad Sclavo Diagn. 1985 Mar;21(1):62-70. Unique Identifier : AIDSLINE MED/86043323
Galluccio L; Reverberi R; Castellani A; Ferrari L; Ferrari R; Moretti M; Menini C

Abstract: An enzyme-linked antiglobulin test (ELAT) is described. All reagents are commercially available. The antiglobulin reagent is a monoclonal anti-IgG conjugated with alkaline phosphatase. The substrate (p-nitrophenylphosphate) is stable and innocuous. The procedure requires long incubations, particularly between red cells and the enzyme-conjugated antibody. However most of the time actively spent by the technician is consumed by washing procedures (LAV). These may be avoided by using density gradient centrifugation (CSG). ELAT is significantly more sensitive than agglutination, using either washing or gradient centrifugation (P = 0.002 and P = 0.0005, respectively). Moreover ELAT-CSG is significantly more sensitive than ELAT-LAV (P = 0.03). CSG yields better reproducibility and probably avoids some of the spontaneous elution of the primary and/or secondary antibody which may occur during washing.
Keywords: Alkaline Phosphatase/METABOLISM *Antibodies, Monoclonal Blood Grouping and Crossmatching/*METHODS Centrifugation, Density Gradient Comparative Study English Abstract Enzyme-Linked Immunosorbent Assay Hemagglutination Tests Human IgG/*IMMUNOLOGY Nitrophenols/METABOLISM Organophosphorus Compounds/METABOLISM Support, Non-U.S. Gov't Time Factors JOURNAL ARTICLE

KWDalkalinephosphatase/metabolismKWDantibodies,monoclonalbloodgroupingandcrossmatching/KWDmethodscentrifugation,densitygradientcomparativestudyenglishabstractenzyme-linkedimmunosorbentassayhemagglutinationtestshumanigg/KWDimmunologynitrophenols/metabolismorganophosphoruscompounds/metabolismsupport,non-uKWDsKWDgov'ttimefactorsjournalarticle
860228
M8620168

Copyright © 1986 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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